WO2001044276A3 - Genes de mousse de physcomitrella patens codant pour des proteines impliquees dans la synthese de tocopherols et de carotenoides - Google Patents

Genes de mousse de physcomitrella patens codant pour des proteines impliquees dans la synthese de tocopherols et de carotenoides Download PDF

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Publication number
WO2001044276A3
WO2001044276A3 PCT/EP2000/012698 EP0012698W WO0144276A3 WO 2001044276 A3 WO2001044276 A3 WO 2001044276A3 EP 0012698 W EP0012698 W EP 0012698W WO 0144276 A3 WO0144276 A3 WO 0144276A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
physcomitrella patens
tocopherols
carotenoids
Prior art date
Application number
PCT/EP2000/012698
Other languages
English (en)
Other versions
WO2001044276A2 (fr
Inventor
Jens Lerchl
Andreas Renz
Thomas Ehrhardt
Andreas Reindl
Petra Cirpus
Friedrich Bischoff
Markus Frank
Annette Freund
Elke Duwenig
Ralf-Michael Schmidt
Ralf Reski
Ralf Badur
Original Assignee
Basf Plant Science Gmbh
Jens Lerchl
Andreas Renz
Thomas Ehrhardt
Andreas Reindl
Petra Cirpus
Friedrich Bischoff
Markus Frank
Annette Freund
Elke Duwenig
Schmidt Ralf Michael
Ralf Reski
Ralf Badur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh, Jens Lerchl, Andreas Renz, Thomas Ehrhardt, Andreas Reindl, Petra Cirpus, Friedrich Bischoff, Markus Frank, Annette Freund, Elke Duwenig, Schmidt Ralf Michael, Ralf Reski, Ralf Badur filed Critical Basf Plant Science Gmbh
Priority to BR0016432-1A priority Critical patent/BR0016432A/pt
Priority to EP00983319A priority patent/EP1244696A2/fr
Priority to AU20098/01A priority patent/AU2009801A/en
Priority to CA002394414A priority patent/CA2394414A1/fr
Publication of WO2001044276A2 publication Critical patent/WO2001044276A2/fr
Publication of WO2001044276A3 publication Critical patent/WO2001044276A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne des molécules d'acide nucléique isolées, dénommées molécules d'acide nucléique de protéines associées au métabolisme de tocophérols et de caroténoïdes, codant pour de nouvelles protéines associées au métabolisme de tocophérols et de caroténoïdes de Physcomitrella patens. L'invention concerne également des molécules d'acide nucléique anti-sens, des vecteurs d'expression de recombinaison contenant des molécules d'acide nucléique de protéines associées au métabolisme de tocophérols et de caroténoïdes, ainsi que des cellules hôtes dans lesquelles les vecteurs d'expression ont été introduits. L'invention concerne, enfin, des protéines associées au métabolisme de tocophérols et de caroténoïdes isolés, des protéines associées au métabolisme de tocophérols et de caroténoïdes mutés, des protéines fusionnées, des peptides antigéniques et des procédés destinés à améliorer la production d'un composé désiré à partir de cellules transformées, d'organismes ou de plantes fondées sur le génie génétique de gènes de protéines associées au métabolisme de tocophérols et de caroténoïdes dans ces organismes.
PCT/EP2000/012698 1999-12-16 2000-12-14 Genes de mousse de physcomitrella patens codant pour des proteines impliquees dans la synthese de tocopherols et de carotenoides WO2001044276A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
BR0016432-1A BR0016432A (pt) 1999-12-16 2000-12-14 Molécula de ácido nucleico isolada, vetor, célula hospedeira, descendentes, sementes ou material celular reproduzìvel, método de produção de um ou mais polipeptìdeo(s), tcmrp isolada, polipeptìdeo isolado, anticorpo, kit de teste, método para produzir um produto quìmico fino, produto quìmico fino, e, uso de um produto quìmico fino ou de polipeptìdeo(s).
EP00983319A EP1244696A2 (fr) 1999-12-16 2000-12-14 Genes de mousse de physcomitrella patens codant pour des proteines impliquees dans la synthese de tocopherols et de carotenoides
AU20098/01A AU2009801A (en) 1999-12-16 2000-12-14 Moss genes from physcomitrella patens encoding proteins involved in the synthesis of tocopherols carotenoids and aromatic amino acids
CA002394414A CA2394414A1 (fr) 1999-12-16 2000-12-14 Genes de mousse de physcomitrella patens codant pour des proteines impliquees dans la synthese de tocopherols et de carotenoides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17112199P 1999-12-16 1999-12-16
US60/171,121 1999-12-16

Publications (2)

Publication Number Publication Date
WO2001044276A2 WO2001044276A2 (fr) 2001-06-21
WO2001044276A3 true WO2001044276A3 (fr) 2001-11-08

Family

ID=22622617

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/012698 WO2001044276A2 (fr) 1999-12-16 2000-12-14 Genes de mousse de physcomitrella patens codant pour des proteines impliquees dans la synthese de tocopherols et de carotenoides

Country Status (6)

Country Link
EP (1) EP1244696A2 (fr)
AR (1) AR026983A1 (fr)
AU (1) AU2009801A (fr)
BR (1) BR0016432A (fr)
CA (1) CA2394414A1 (fr)
WO (1) WO2001044276A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1171610B1 (fr) 1999-04-15 2007-04-04 Calgene LLC Sequences d'acide nucleique et proteines intervenant dans la synthese de l'isoprenoide
US6872815B1 (en) 2000-10-14 2005-03-29 Calgene Llc Nucleic acid sequences to proteins involved in tocopherol synthesis
EP1356033B1 (fr) 2000-08-07 2009-10-28 Monsanto Technology LLC Genes de la voie methyl-d-erythritol phosphate
CN100372939C (zh) * 2000-10-06 2008-03-05 札幌啤酒株式会社 法尼焦磷酸合酶蛋白质、核酸及其启动子区域
DE60230608D1 (de) 2001-05-09 2009-02-12 Monsanto Technology Llc Tyra-gene und ihre verwendung
US7161061B2 (en) 2001-05-09 2007-01-09 Monsanto Technology Llc Metabolite transporters
EP1897952A3 (fr) * 2001-08-17 2009-12-09 Monsanto Technology, LLC Méthyltransférasegénes et leurs utilisations
EP1427832A4 (fr) * 2001-08-17 2006-09-06 Monsanto Technology Llc Genes de methyltransferase et leurs utilisations
BR0213499A (pt) 2001-10-25 2004-11-03 Monsanto Technology Llc Metiltransferases aromáticas e seu uso
BRPI0308740B1 (pt) 2002-03-19 2018-11-21 Monsanto Technology Llc molécula de ácido nucléico codificando uma homogentisato prenil transferase (“hpt”) e método de produzir planta tendo semente com nível de tocoferol aumentado
US7230165B2 (en) 2002-08-05 2007-06-12 Monsanto Technology Llc Tocopherol biosynthesis related genes and uses thereof
US7663021B2 (en) 2002-12-06 2010-02-16 Del Monte Fresh Produce Company Transgenic pineapple plants with modified carotenoid levels and methods of their production
WO2005014828A2 (fr) * 2003-08-01 2005-02-17 Basf Plant Science Gmbh Procede de production de composes chimiques fins
US8008545B2 (en) 2003-04-15 2011-08-30 Basf Plant Science Gmbh Process for the production of fine chemicals
UA94038C2 (ru) 2005-03-18 2011-04-11 Майкробиа, Инк. Продуцирование каротиноидов в маслянистых дрожжах и грибах
EP2078092A2 (fr) 2006-09-28 2009-07-15 Microbia, Inc. Production de caroténoïdes dans des levures et des champignons oléagineux

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 24 November 1993 (1993-11-24), EBERHARD ET AL.: "Lycopersicon esculentum chorismate mutase mRNA", XP002163767 *
DATABASE EMBL EBERHARD ET AL.: "A. thaliana mRNA for chorismate mutase", XP002163766 *
EBERHARD JENNY ET AL: "Cloning and expression in yeast of a higher plant chorismate mutase: Molecular cloning, sequencing of the cDNA and characterization of the Arabidopsis thaliana enzyme expressed in yeast.", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) LETTERS, vol. 334, no. 2, 1993, pages 233 - 236, XP000985166, ISSN: 0014-5793 *
EBERHARD JENNY ET AL: "Isolation of a cDNA from tomato coding for an unregulated, cytosolic chorismate mutase.", PLANT MOLECULAR BIOLOGY, vol. 31, no. 4, 1996, pages 917 - 922, XP000985279, ISSN: 0167-4412 *
HIRSCHBERG JOSEPH: "Production of high-value compounds: Carotenoids and vitamin E.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 10, no. 2, April 1999 (1999-04-01), pages 186 - 191, XP002162837, ISSN: 0958-1669 *
PUCHTA H: "Towards targeted transformation in plants", TRENDS IN PLANT SCIENCE, (MAR 1998) VOL. 3, NO. 3, PP. 77-78. ISSN: 1360-1385., XP002162373 *
RESKI R: "MOLECULAR GENETICS OF PHYSCOMITRELLA", PLANTA,SPRINGER VERLAG,DE, vol. 208, no. 3, May 1999 (1999-05-01), pages 301 - 309, XP000881434, ISSN: 0032-0935 *
RESKI R: "PHYSCOMITRELLA AND ARABIDOPSIS: THE DAVID AND GOLIATH OF REVERSE GENETICS", TRENDS IN PLANT SCIENCE,ELSEVIER SCIENCE, OXFORD,GB, vol. 3, no. 6, June 1998 (1998-06-01), pages 209 - 210-1385, XP000881435, ISSN: 1360-1385 *
RESKI, R. (1) ET AL: "Moss (Physcomitrella patens) expressed sequence tags include several sequences which are novel for plants.", BOTANICA ACTA, (APRIL, 1998) VOL. 111, NO. 2, PP. 143-149., XP000985074 *
RESKI, R. (1): "Development, genetics and molecular biology of mosses.", BOTANICA ACTA, (FEB., 1998) VOL. 111, NO. 1, PP. 1-15., XP000985073 *

Also Published As

Publication number Publication date
BR0016432A (pt) 2002-09-17
AR026983A1 (es) 2003-03-05
EP1244696A2 (fr) 2002-10-02
WO2001044276A2 (fr) 2001-06-21
AU2009801A (en) 2001-06-25
CA2394414A1 (fr) 2001-06-21

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