BR0111907A - Construção para a expressão de um gene, vetor, célula hospedeira, processo de produção de uma proteìna recombinante de interesse em uma célula hospedeira e utilização de uma construção e de uma proteìna recombinante - Google Patents
Construção para a expressão de um gene, vetor, célula hospedeira, processo de produção de uma proteìna recombinante de interesse em uma célula hospedeira e utilização de uma construção e de uma proteìna recombinanteInfo
- Publication number
- BR0111907A BR0111907A BR0111907-9A BR0111907A BR0111907A BR 0111907 A BR0111907 A BR 0111907A BR 0111907 A BR0111907 A BR 0111907A BR 0111907 A BR0111907 A BR 0111907A
- Authority
- BR
- Brazil
- Prior art keywords
- recombinant protein
- host cell
- construct
- interest
- vector
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 7
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 7
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 238000010276 construction Methods 0.000 abstract 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 abstract 1
- 108091081024 Start codon Proteins 0.000 abstract 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
"CONSTRUçãO PARA A EXPRESSãO DE UM GENE, VETOR, CéLULA HOSPEDEIRA, PROCESSO DE PRODUçãO DE UMA PROTEìNA RECOMBINANTE DE INTERESSE EM UMA CéLULA HOSPEDEIRA E UTILIZAçãO DE UMA CONSTRUçãO E DE UMA PROTEìNA RECOMBINANTE". A invenção refere-se a uma construção para a expressão de um gene codificando para uma proteína recombinante de interesse colocada sob o controle de operon triptofano Ptrp em uma célula hospedeira procariota, compreendendo diretamente a jusante do códon de iniciação uma seq³ência nucleica de seq³ência SEQ ID NO: 1, e a jusante desta seq³ência um cassete de clonagem múltipla destinado a receber o gene codificando para a referida proteína recombinante de interesse, pelo menos um dos nucleotídeos da seq³ência SEQ ID NO: 1 sendo mudado ou deletado de modo a permitir uma super-expressão da referida proteína recombinante. A invenção tem ainda por objeto um vetor contendo uma tal construção, uma célula hospedeira procariota transformada pelo referido vetor, assim como um processo de produção de uma proteína recombinante de interesse usando uma construção de acordo com a invenção.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0008002A FR2810675B1 (fr) | 2000-06-22 | 2000-06-22 | Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes |
| PCT/FR2001/001952 WO2001098453A2 (fr) | 2000-06-22 | 2001-06-21 | Vecteurs d'expression comprenant un fragment modifie de l'operon tryptophane |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0111907A true BR0111907A (pt) | 2003-12-30 |
Family
ID=8851556
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0111907-9A BR0111907A (pt) | 2000-06-22 | 2001-06-21 | Construção para a expressão de um gene, vetor, célula hospedeira, processo de produção de uma proteìna recombinante de interesse em uma célula hospedeira e utilização de uma construção e de uma proteìna recombinante |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20040260060A1 (pt) |
| EP (1) | EP1315822A2 (pt) |
| JP (1) | JP2004500875A (pt) |
| CN (1) | CN1443242A (pt) |
| AU (1) | AU6922401A (pt) |
| BR (1) | BR0111907A (pt) |
| CA (1) | CA2413612A1 (pt) |
| FR (1) | FR2810675B1 (pt) |
| MX (1) | MXPA02012880A (pt) |
| WO (1) | WO2001098453A2 (pt) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7691989B2 (en) | 2004-12-22 | 2010-04-06 | Genentech, Inc. | Methods for producing soluble membrane-spanning proteins |
| EP2185704A1 (en) * | 2007-08-03 | 2010-05-19 | Pfenex, Inc. | Translation initiation region sequences for the optimal expression of heterologous proteins |
| KR100961565B1 (ko) * | 2008-03-10 | 2010-06-07 | 충남대학교산학협력단 | 목적하는 단백질 발현 수준을 제공하는 초기 코돈의발굴방법, 및 이를 이용한 재조합 단백질의 발현 조절 방법및 생산 방법 |
| WO2009135056A2 (en) * | 2008-04-30 | 2009-11-05 | Monsanto Technology Llc | Recombinant dna vectors for expression of human prolactin antagonists |
| AU2009266989B2 (en) * | 2008-07-03 | 2013-05-02 | Pfenex, Inc. | High throughput screening method and use thereof to identify a production platform for a multifunctional binding protein |
| CN110491447B (zh) * | 2019-08-05 | 2021-08-17 | 浙江省农业科学院 | 一种用于异源基因体外表达的密码子优化方法及应用 |
| CN115960934A (zh) * | 2022-08-24 | 2023-04-14 | 深圳柏垠生物科技有限公司 | 大肠杆菌表达外源基因优化方法及其序列 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4738921A (en) * | 1984-09-27 | 1988-04-19 | Eli Lilly And Company | Derivative of the tryptophan operon for expression of fused gene products |
| US4772555A (en) * | 1985-03-27 | 1988-09-20 | Genentech, Inc. | Dedicated ribosomes and their use |
| US5149792A (en) * | 1989-12-19 | 1992-09-22 | Amgen Inc. | Platelet-derived growth factor B chain analogs |
| US6121416A (en) * | 1997-04-04 | 2000-09-19 | Genentech, Inc. | Insulin-like growth factor agonist molecules |
| GB9716790D0 (en) * | 1997-08-07 | 1997-10-15 | Creative Peptides Sweden Ab | Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof |
-
2000
- 2000-06-22 FR FR0008002A patent/FR2810675B1/fr not_active Expired - Fee Related
-
2001
- 2001-06-21 WO PCT/FR2001/001952 patent/WO2001098453A2/fr not_active Application Discontinuation
- 2001-06-21 BR BR0111907-9A patent/BR0111907A/pt not_active IP Right Cessation
- 2001-06-21 MX MXPA02012880A patent/MXPA02012880A/es unknown
- 2001-06-21 CA CA002413612A patent/CA2413612A1/fr not_active Abandoned
- 2001-06-21 AU AU69224/01A patent/AU6922401A/en not_active Abandoned
- 2001-06-21 CN CN01812988.9A patent/CN1443242A/zh active Pending
- 2001-06-21 EP EP01947565A patent/EP1315822A2/fr not_active Withdrawn
- 2001-06-21 JP JP2002504602A patent/JP2004500875A/ja active Pending
- 2001-06-21 US US10/311,976 patent/US20040260060A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004500875A (ja) | 2004-01-15 |
| AU6922401A (en) | 2002-01-02 |
| WO2001098453A2 (fr) | 2001-12-27 |
| CN1443242A (zh) | 2003-09-17 |
| FR2810675A1 (fr) | 2001-12-28 |
| US20040260060A1 (en) | 2004-12-23 |
| WO2001098453A3 (fr) | 2002-08-01 |
| CA2413612A1 (fr) | 2001-12-27 |
| EP1315822A2 (fr) | 2003-06-04 |
| MXPA02012880A (es) | 2003-05-14 |
| FR2810675B1 (fr) | 2002-09-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 5A, 6A E 7A ANUIDADES. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1963 DE 19.08.2008. |