WO2001094554A1 - Procede de reconstruction genetique d'organes humains - Google Patents

Procede de reconstruction genetique d'organes humains Download PDF

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Publication number
WO2001094554A1
WO2001094554A1 PCT/EP2000/005311 EP0005311W WO0194554A1 WO 2001094554 A1 WO2001094554 A1 WO 2001094554A1 EP 0005311 W EP0005311 W EP 0005311W WO 0194554 A1 WO0194554 A1 WO 0194554A1
Authority
WO
WIPO (PCT)
Prior art keywords
zeee
haploid
chromosome
reconstruction
chromosomes
Prior art date
Application number
PCT/EP2000/005311
Other languages
German (de)
English (en)
Inventor
Dieter Vogl
Original Assignee
Trostner, Jens
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trostner, Jens filed Critical Trostner, Jens
Priority to AU2000250770A priority Critical patent/AU2000250770A1/en
Priority to GB0228588A priority patent/GB2380202A/en
Priority to PCT/EP2000/005311 priority patent/WO2001094554A1/fr
Priority to DE10084883T priority patent/DE10084883D2/de
Publication of WO2001094554A1 publication Critical patent/WO2001094554A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

Definitions

  • Cloning organisms is known.
  • any body cells of a primary Oiganism and un-fertilized egg cells of the same type are -k-i-seeded.
  • the cells come from the organism to be cloned.
  • the entire I ⁇ -formation is then sucked out of an egg cell with a pointed hollow needle.
  • the egg cell is held in place by a larger blunt hollow needle by vacuum.
  • a body cell with intact genetic information is picked up and injected as a whole into the emptied egg cell.
  • the body cell penetrates the egg cell and then the process of fusion begins.
  • the egg cell must also be placed between two thin wires that conduct electricity.
  • the preciseness of the new life begins, the result of which is a living being that is completely identical to the primary organism.
  • genetic engineering has so far not been possible to use individual organs of living beings in their specific form and Duplicate function identically.
  • the invention specified in the patent claim is based on the problem of genetically influencing the erbirifomiations in human cells in such a way that organs develop in their specific form and function, without an organism as a whole having to be cloned.
  • a cell is removed from a human organism for which a particular organ is to be cloned (in addition to somatic cells, germ cells are best suited for the procedure).
  • the removed cell is then placed in a nutrient solution and stimulated to divide using one of the currently usual methods of division. After this process, an entire organism would be formed, but this should be prevented during the genetic reconstruction of individual organs at the time of prophase, when the chromosome threads become denser and become visible, the cell contains those C chromosomes that are not responsible for a particular organ.
  • the ones to be removed Chromosomes are determined according to the internationally standardized karyogram (Denver Convention 1960 - 22 autosome pairs; 1 pair of heterochromosomes; their size in 7 groups)
  • the three basic chromosomes are autosome pairs 1, 13 and 21. They may only be haploid (with three exceptions, which are described below).
  • the chromosome which is additionally required and dependent on the oigan, must either be left diploid or haploid in the cell.
  • the marketing of the invention is given because the organs to be genetically reconstructed can be cloned at a reasonable fee and can be used, for example, in the context of traditional translational technology.
  • the advantages obtained with the invention are, in particular, that the organs, which are identically reproduced on the basis of the genetic method of selective toilet, are available for a transplant within a very short time.
  • the previous dependency on organs from a genetically compatible foreign donor no longer exists.
  • the high risk of rejection of transplanted foreign organs by the body's own immune system especially because every cell in an organism is able to differentiate between the body's own (soapy) and body-internal (not self), is excluded in the new microbiological process of genetic reconstruction , This means that no drugs suppressing the body's immune system are required.
  • the transplant's life-threatening side effects and drug interactions are consequently eliminated. •
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14 are removed from the cell, which is still in the prophase stage , 15, 17, 18, 19, 20 and 22 as well as the gender echo chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 16, which is responsible for the specific reconstruction of the liver, is also left haploid in the ZeEe. The further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the sex chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell.
  • the chromosome 11 responsible for the speafic reconstruction of the spleen is also left haploid in the cell.
  • the further implementation follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 12, which is responsible for the specific reconstruction of the biliary nucleus, is also left haploid in the cell. The further execution follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the GescMecfotsc chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell.
  • Chromosome 14, which is responsible for the specific reconstruction of the intestines, is also left haploid in the cell. The further embodiment follows the description of claim 1.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 8, which is responsible for the specific reconstruction of the right hand, is also left haploid in the cell. The further execution follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the sex chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 10, which is responsible for the specific reconstruction of the left hand, is also left haploid in the cell. The further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the G - bad chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 9, which is responsible for the speafic reconstruction of the left kidney, is also left haploid in the cell. The further execution follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the GescWec-chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell.
  • the further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 and the sex chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell.
  • Chromosome 5, which is responsible for the specific construction of the right leg, is also left haploid in the cell.
  • the further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 and the sex chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 7, which is responsible for the specific reconstruction of the left leg, is also left haploid in the cell. The further embodiment follows the description of claim 1.
  • the chromosome pairs 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the Gesc-M - cht - chr ⁇ moso ⁇ en.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 2, which is responsible for the specific reconstruction of the right eye, is left diploid in the cell. The further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 are removed from the cell, which is still in the stage of the prophase. 12, 14, 15, 16, 17, 18, 20 and 22 as well as the G ⁇ sch --- ht-schromosomen.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 19, which is responsible for the specific reconstruction of the left eye, is left diploid in the cell. The further implementation follows the description of patent claim 1.
  • the chromosome pairs 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 and the sex chromosomes.
  • the chromosomes 1, 13 and 21 are left haploid in the cell.
  • the chromosome 3 responsible for the specific construction of the right ear is left diploid in the Cell. The further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14 are removed from the cell, which is still in the prophase stage , 15, 16, 18, 19, 20 and 22 as well as the Ge - CM-5cht Schromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 17, which is responsible for the specific reconstruction of the left ear, is left diploid in the cell. The further execution follows the description of claim 1. " ⁇ "
  • the chromosome pairs 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15 are removed from the cell, which is still in the prophase stage , 16, 17, 18, 19, 20 and 22 as well as the Gesc-hl-x ⁇ ⁇ tschromosomen.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 4, which is responsible for the specific reconstruction of the right half of the nose, is left diploid in the cell. The further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14 are removed from the cell, which is still in the prophase stage , 15, 16, 17, 18, 19, and 22 as well as the Geschl-x-htechromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell.
  • the chromosome 20 responsible for the specific reconstruction of the left half of the nose is left diploid in the cell.
  • the further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14 are removed from the cell, which is still in the prophase stage , 15, 16, 17, 18, 19 and 20 as well as the sex chromosomes.
  • Chromosomes 1, 13 and 21 are left haploid in the cell. Chromosome 22, which is responsible for the specific reconstruction of the mouth, is left diploid in the cell.
  • the further embodiment follows the description of claim 1. • Microbiological procedure for the reconstruction of the organs in the breast
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14 are removed from the cell, which is still in the prophase stage , 15, 16, 17, 18, 19, 20 and 22 as well as the sex chromosomes.
  • Chromosomes 13 and 21 are left haploid in the cell. Chromosome 1, which is responsible for the specific reconstruction of the breast, is left diploid in the cell. The further embodiment follows the description of claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14 are removed from the cell, which is still in the prophase stage , 15, 16, 17, 18, 19, 20 and 22 as well as the sex chromosomes.
  • Chromosomes 1 and 21 are left haploid in the cell. Chromosome 13, which is responsible for the specific reconstruction of the organs in the abdomen, is left diploid in the cell. The further execution follows the description of F&T claim 1.
  • the chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14 are removed from the cell, which is still in the prophase stage , 15, 16, 17, 18, 19, 20 and 22 as well as the sex chromosomes.
  • Chromosomes 1 and 13 are left haploid in the cell. Chromosome 21, which is responsible for the specific reconstruction of the organs in the head, is left diploid in the cell. The further embodiment follows the description of claim 1.
  • Cloning organisms is known. In a Petri dish with a nutrient solution, any body cells of a Pr- ⁇ -närorganism and unhumidified egg cells of the same kind are cultivated. The cells come from the organism that is to be cloned to graze. The entire genetic information is then sucked out of an egg cell with a pointed hollow needle. The egg cell is held in place by a larger blunt hollow needle by vacuum. Then, with another hollow needle, a body cell with intact genetic information is taken up and injected as a whole into the empty egg cell. The body parts penetrate into the egg cell and then the process of fusion begins. In order to promote this process, the egg cell must also be placed between two thin wires carrying st-rom.
  • the ----- kfindung specified in the claim is based on the problem of genetically influencing the genetic information in human germ cells and cells in such a way that organs develop in their specific form and function without an organism as a whole having to be cloned.
  • a cell is removed from a human organism for which a particular organ is to be cloned (in addition to such atic cells, germ cells are best suited for the procedure).
  • the removed cell is then placed in a nutrient solution and stimulated to divide using one of the currently usual methods of division.
  • an entire organism would arise, but this should be prevented during the genetic reconstruction of individual organs at the time of prophase, when the cbromosome threads become denser and become visible, all the chromosomes in the cell and are sized according to the internationally standardized one Karyogram (Denver Convention 1960 - 22 autosome pairs; 1 pair of heterochrornosomes; according to their size - in 7 groups).
  • the chromosomes required for the reconstruction are then carried out according to the formula
  • the three basic chromosomes are the autosome pairs 1, 13 and 21. They may only be haploid (with three exceptions, which are described below).
  • the additional chromosome, which is required depending on the organ, is either diploid or haploid.
  • the invention is marketed because the organs to be genetically reconstructed can be used for a reasonable fee and can be used, for example, in the context of traditional transplantation techniques.
  • the advantages achieved by the invention are, in particular, that the organs which are reconstructed identically on the basis of the genetic method of selective cloning are available for a transplant within a very short time.
  • the previous dependency on organs from a genetically compatible foreign donor no longer exists.
  • the high risk of rejection of trans- ⁇ lanted foreign organs by the body's own immune system, especially because every cell in an organism is able to differentiate between the body's own (soapy) and foreign (not self), is the result of the new molecular biological process of genetic reconstruction ausg- ⁇ chlossen. This means that no drugs that defend the body's immune system are needed. The transplant's life-threatening side effects and drug interactions are therefore eliminated. • MoleJ- ⁇ i-l-arbiolcgi- ⁇ hesVei ⁇
  • the germ cell or the cell which is still in the "slowed down" stage of prophase, has chromosomes 1, 13 and 21 (haploid) and the chromosome for the specific reconstruction of the liver 16 also haploid too.
  • the further embodiment follows the description of claim 2.
  • the germ cell or cell which is still in the "slowed down" stage of prophase, has chromosomes 1, 13 and 21 (haploid) and chromosome 14 for the specific reconstruction of the intestines also haploid too.
  • the further embodiment follows the description of claim 2.
  • the germ cell or cell which is still in the "slowed down" stage of prophase, has chromosomes 1, 13 and 21 (haploid) and the chromosome for the specific reconstruction of the left kidney 9 also haploid too.
  • the further embodiment follows the description of claim 2.
  • the germ cell or cell which is still in the "slowed down" stage of prophase, has chromosomes 1, 13 and 21 (haploid) and the chromosome for the specific reconstruction of the right kidney 6 also haploid too.
  • the further embodiment follows the description of claim 2.
  • the germ cell or cell which is still in the "slowed down" stage of prophase, has chromosomes 1, 13 and 21 (haploid) and the chromosome for the specific reconstruction of the right eye 2 diploid too.
  • the further execution follows the -Eksdireibi-ing des -Patent - ⁇ nspruchs 2.
  • the germ cell or cell which is still in the "slowed down" stage of prophase, has chromosomes 1, 13 and 21 (haploid) and the chromosome for the specific reconstruction of the right half of the nose 4 diploid too.
  • the further execution follows the description of the rate claim 2.
  • the germ cell or cell which is still in the "slowed down" stage of prophase, has chromosomes 1, 13 and 21 (haploid) and the chromosome for the specific reconstruction of the left half of the nose 20 diploid too.
  • the rest of the execution follows the description of the claim 2.
  • the germ cell or cell which is still in the "slowed down” stage of prophase, has chromosomes 1, 13 and 21 (haploid) and chromosome 22 for the specific reconstruction of the mouth diploid too.
  • the further execution follows the description of claim 2. • Molecular biological method for the reconstruction of the organs in the breast According to the basic formula ((3) + 1), the germ cell or cell, which is still in the "decelerated” stage of the prophase, is led to chromosomes 13 and 21 (haploid) and for specific reconstruction of organs in a breast chromosome 1 diploid too.
  • the further extension follows the description of patent claim 2.

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  • Life Sciences & Earth Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
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Abstract

L'invention concerne un procédé microbiologique de reconstruction génétique d'organes humains, selon lequel on utilise des cellules endogènes et les informations héréditaires qu'elles contiennent.
PCT/EP2000/005311 2000-06-08 2000-06-08 Procede de reconstruction genetique d'organes humains WO2001094554A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2000250770A AU2000250770A1 (en) 2000-06-08 2000-06-08 Method for the genetic reconstruction of human organs
GB0228588A GB2380202A (en) 2000-06-08 2000-06-08 Method for the genetic reconstruction of human organs
PCT/EP2000/005311 WO2001094554A1 (fr) 2000-06-08 2000-06-08 Procede de reconstruction genetique d'organes humains
DE10084883T DE10084883D2 (de) 2000-06-08 2000-06-08 Verfahren zur genetischen Rekonstruktion von menschlichen Organen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2000/005311 WO2001094554A1 (fr) 2000-06-08 2000-06-08 Procede de reconstruction genetique d'organes humains

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WO2001094554A1 true WO2001094554A1 (fr) 2001-12-13

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AU (1) AU2000250770A1 (fr)
DE (1) DE10084883D2 (fr)
GB (1) GB2380202A (fr)
WO (1) WO2001094554A1 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1195699A (zh) * 1997-09-22 1998-10-14 任春严 移植所需器官的生产和器官修复·再造的方法和装置
WO1999001163A1 (fr) * 1997-07-03 1999-01-14 University Of Massachusetts Clonage a l'aide de noyaux donneurs a partir de cellules differentiees ne presentant pas de carence serique

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999001163A1 (fr) * 1997-07-03 1999-01-14 University Of Massachusetts Clonage a l'aide de noyaux donneurs a partir de cellules differentiees ne presentant pas de carence serique
CN1195699A (zh) * 1997-09-22 1998-10-14 任春严 移植所需器官的生产和器官修复·再造的方法和装置

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199909, Derwent World Patents Index; Class B04, AN 1999-096571, XP002166993 *

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AU2000250770A1 (en) 2001-12-17
GB0228588D0 (en) 2003-01-15
GB2380202A (en) 2003-04-02
DE10084883D2 (de) 2004-04-29

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