GB2380202A - Method for the genetic reconstruction of human organs - Google Patents

Method for the genetic reconstruction of human organs Download PDF

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GB2380202A
GB2380202A GB0228588A GB0228588A GB2380202A GB 2380202 A GB2380202 A GB 2380202A GB 0228588 A GB0228588 A GB 0228588A GB 0228588 A GB0228588 A GB 0228588A GB 2380202 A GB2380202 A GB 2380202A
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cell
reconstruction
haploid
chromosomes
chromosome
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Dieter Vogl
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TROSTNER JENS
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TROSTNER JENS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor

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  • Genetics & Genomics (AREA)
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Abstract

The invention relates to a microbiological method for the genetic reconstruction of human organs using endogenous cell and the hereditary information contained therein.

Description

- 1 - PATENT CLAIM 1 - DESCRIPTION
Microbiological method for genetic reconstruction of human organs using endogenous cells and the genetic information contained therein.
Cloning of organisms is known. Any body cells of a primary organism and unfertilized oocytes or egg cells of the same species are cultured in a Petri dish containing a nutrient solution. The cells come from the organism that is to be cloned. Then using a pointed hollow needle, all the genetic information is drawn out of an oocyte. Next, the oocyte is held securely by a vacuum through a larger blunt hollow needle. Following this, an endogenous cell with intact genetic information is taken up with another hollow needle and injected as a whole into the evacuated oocyte. The endogenous cell penetrates into the oocyte and then the process of fusion begins. To promote this process, the oocyte must also be placed between two thin current-
carrying wires. Consequently, according to the information currently available, the process of new life begins, the result of which is a living creature which is completely identical to the primary organism. However, it has not previously been possible for genetic engineering to duplicate individual organs of living creatures that are identical in their specific form and function.
The invention characterized in the patent claim is based on the problem of influencing the genetic information in human cells genetically so that organs develop in their specific form and function without having to clone an organism as a whole. The solution to this problem: first, a cell is taken from a human organism for which a certain organ is to be cloned (in addition to somatic cells, germ cells or gametes are best suited for this method). The cell removed is then i i i
- 2 placed in a nutrient solution and stimulated to m- osis by means of a method which is conventional today. Since an entire organism would be created by this method, but this is to be prevented in genetic reconstruction of Individual organs, the chromosomes that are not responsible for a certain organ are removed from the cell at the mist cell division at the prophase time, when compress of of the chromosome strands occurs and these become visible. The chromosomes to be removed are determined accoroi. to the internationally standardized karyogram (Coerce ion of Denver 1960 - 22 autosomal pairs; 1 pair of heterochromosomes; in 7 groups according to sin_).
The formula for the microbiological process o genetic reconstruction of organs is given as follows: ((3)+1)
The three basic chromosomes are the autosomal pairs 1, 13 and 21. They must always be present only as haploid cells (with three exceptions, which are described below). The additional chromosome required, depending on the organ, is left in either a haploid or diploid form in the cell.
After this genetic engineering procedure, the cell is placed in a container with circulating nutrient solution and is stimulated to cell division with an input of energy according to traditional methods, which then leads to cell replication. During this process, a specific differentiation of the cells occurs, resulting ultimately in development of the form and function of the respective organ. Marketing of this invention is ensured because the organs to be reconstructed genetically are cloned by assessing an À appropriate fee and may be used as part of traditional transplantation technology, for example.
..
- 3 The advantages achieved with this invention consist in particular of the fact that these organs, which have been reconstructed identically on the basis of the genetic method of selective cloning, are available for transplantation within an extremely short period of time.
This eliminates the previous dependence on organs of the most genetically compatible foreign donor possible. The high risk of rejection of transplanted foreign organs due to the endogenous immune system is ruled out with the new microbiological method of genetic reconstruction, in particular because each cell of an organism is capable of differentiating between endogenous (self) and exogenous (not self). It is thus also unnecessary to use medications that suppress the endogenous immune system. Therefore, this eliminates life-threatening side effects and drug interactions for the person receiving the transplant.
À Microbiological method of reconstruction of the liver According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 17, 18, 19, 20 and 22 as well as the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 16, which is responsible for specific reconstruction of the liver, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the spleen According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 15, 16, 17, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 11, which is responsible for specific reconstruction of the spleen, is also left in haploid form in the cell. The remaining procedure follows the : (. . c
description of Patent Claim 1.
À Microbiological method of reconstruction of the gallbladder According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 14, 15, 16, 17, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 12, which is responsible for the specific reconstruction of the gallbladder, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the intestine According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 15, 16, 17, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 14, which is responsible for specific reconstruction of the intestine, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right hand According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 8, which is responsible for the specific reconstruction of the right hand, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
... i i.. -.
. ..
À Microbiological method of reconstruction of the left hand According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15, 16, 17, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 10, which is responsible for the specific reconstruction of the left hand, also remains in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the left kidney According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 9, which is responsible for the specific reconstruction of the left kidney, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right kidney According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 6, which is responsible for the specific reconstruction of the right kidney, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right leg According to the basic formula ((3)+1), chromosome pairs 2, i.
- 6 - 3, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 5, which is responsible for specific reconstruction of the right leg, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the left leg According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 7, which is responsible for the specific reconstruction of the left leg, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right eye According to the basic formula ((3)+1), chromosome pairs 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 2, which is responsible for specific reconstruction of the right eye, is left in diploid form in the cell. The remaining procedure follows the description
of Patent Claim 1.
À Microbiological method of reconstruction of the left eye According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the : ... ,.
- 7 cell. Chromosome 19, which is responsible for the specific reconstruction of the left eye, is left in diploid form in the cell. The remaining procedure follows the description
of Patent Claim 1.
À Microbiological method of reconstruction of the right ear According to the basic formula ((3)+1), chromosome pairs 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 3, which is responsible for specific reconstruction of the right ear, is left in diploid form in the cell. The remaining procedure follows the description
of Patent Claim 1.
À Microbiological method of reconstruction of the left ear According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 17, which is responsible for the specific reconstruction of the left ear, is left in diploid form in the cell. The remaining procedure follows the description
of Patent Claim 1.
À Microbiological method of reconstruction of the right half of the nose According to the basic formula ((3)+1), chromosome pairs 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 4, which is responsible for the specific reconstruction of the right half of the nose, is left in diploid form in the cell. The remaining procedure follows . ( (, ; (.
; ( c .
- 8 the description of Patent Claim 1.
À Microbiological method of reconstruction of the left half of the nose According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 20, which is responsible for the specific reconstruction of the left half of the nose, is left in diploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the mouth According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 and 20 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 22, which is responsible for the specific reconstruction of the mouth, is left in diploid form in the cell. The remaining procedure follows the description of
Patent Claim 1.
À Microbiological method of reconstruction of the organs in the chest According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 13 and 21 are left in haploid form in the cell.
Chromosome 1, which is responsible for the specific reconstruction of the chest, is left in diploid form in the cell. The remaining procedure follows the description of
Patent Claim 1.
..... .. i . (
(.. . to.
_ 9 _ Microbiological method of reconstruction of the organs in the abdomen According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1 and 21 are left in haploid form in the cell.
Chromosome 13, which is responsible for the specific reconstruction of the organs in the abdomen, is left in diploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the organs in the head According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the ceil, which is still in the prophase stage.
Chromosomes 1 and 13 are left in haploid form in the cell.
Chromosome 21, which is responsible for the specific reconstruction of the organs in the head, is left in diploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À, . . , -,
, . . .
PATENT CLAIM 2 - DESCRIPTION
Microbiological method of genetic reconstruction of human organs using endogenous cells and the genetic information contained therein.
Cloning of organisms is known. Any body cells of a primary organism and unfertilized oocytes of the same species are cultured in a Petri dish containing a nutrient solution.
The cells come from the organism to be cloned. Then all the genetic information is drawn out of an oocyte by using a pointed hollow needle. Next, the oocyte is held securely by a vacuum through a larger blunt hollow needle. Following this, another hollow needle is used to pick up an endogenous cell with intact genetic information and inject it as a whole into the evacuated oocyte. The endogenous cell penetrates into the oocyte, and then the process of fusion begins. To promote this process, the oocyte must also be placed between two thin current-carrying wires.
Consequently, according to the information currently available, the process of new life begins, resulting in a living creature that is completely identical to the primary organism. However, it has not previously been possible through genetic engineering to duplicate individual organs of living creatures that are identical in their specific form and function.
The invention characterized in the patent claim is based on the problem of genetically influencing the genetic information in human germ cells so that organs develop in their specific form and function without having to clone an organism as a whole.
The solution to this problem: first, a cell is taken from a human organism for which a certain organ is to be cloned (in addition to somatic cells, germ cells or gametes are best suited for this method). The cell removed is then . .. .....
. . . .. I. (... ...
< c c c
placed in a nutrient solution and stimulated to mitosis by means of a method which is conventional today. An entire organism would be created by this method, but this is to be prevented in genetic reconstruction of individual organs, so the chromosomes that are not responsible for a certain organ are removed from the cell at the first cell division at the prophase, when the chromosome strands are compressed and become visible. The chromosomes to be removed are determined according to the internationally standardized karyogram (Convention of Denver 1960 - 22 autosomal pairs) 1 pair of heterochromosomes; in 7 groups according to size). Then the chromosomes needed for the reconstruction according to the following formula: ((3)+1)
are added to the cell. The three basic chromosomes are the autosomal pairs 1, 13 and 21. They must always be present only as haploid cells (with three exceptions, which are described below). The additional chromosome required, depending on the organ, is restored to either haploid or diploid form. After this genetic engineering procedure, the cell is left in a container with circulating nutrient solution and is stimulated to cell division with an input of energy by traditional methods, which then leads to cell replication. During this process, a specific differentiation of the cells occurs, so that ultimately the form and function of the respective organ can develop.
Marketing of this invention is ensured because the organs to be reconstructed genetically are cloned by assessing an appropriate fee and may be used as part of traditional transplantation technology, for example.
The advantages achieved with this invention consist in particular of the fact that these organs, which have been reconstructed identically on the basis of the genetic : ( À. ; ; c,. c
- 1z method of selective cloning, are available for transplantation within an extremely short period of time.
This eliminates the previous dependence on organs of a foreign donor having the greatest possible genetic compatibility. The high risk of rejection of transplanted foreign organs by the endogenous immune system is ruled out with the new microbiological method of genetic reconstruction, in particular because each cell of an organism is capable of differentiating between endogenous (self) and exogenous (not self). Therefore, it is not necessary to use medication to suppress the endogenous immune system. Therefore, this eliminates life threatening side effects and drug interactions for those receiving such a transplant.
À Microbiological method of reconstruction of the liver According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the liver, chromosome 16, also haploid, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the spleen According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the spleen, chromosome 11, also haploid, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the gallbladder According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the gallbladder, chromosome 12, also haploid, is added to the germ cell or the cell, which is still in the .. . .
i "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the intestine According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the intestine, chromosome 14, also haploid, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure to lows the description of Patent Claim 2.
À Microbiological method of reconstruction of -he right hand According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific recons ruction of the right hand, chromosome 8, also haploid, Is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the left hand According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the left hand, chromosome 10, also haploid, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the left kidney According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the left kidney, chromosome 9, also haploid, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
. ....
.. : ;. .. . i.
- 14 À Microbiological method of reconstruction of the right kidney According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the right kidney, chromosome 6, also haploid, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the right leg According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the right leg, chromosome 5, also haploid, is added to the germ cell or the cell which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the left leg According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the left leg, chromosome 7, also haploid, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the right eye According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the right eye, chromosome 2, also haplo d, is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the left eye According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the left eye, chromosome 19 in diploid form is added to À i <.; ..
. ' ( t. ' t; ..; t. t t t t
- 15 the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the right ear According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the right ear, chromosome 3 in diploid form is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the left ear According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the left ear, chromosome 17 in diploid form is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the right half of the nose According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the right half of the nose, chromosome 4 in diploid form is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the left half of the nose According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the left half of the nose, chromosome 20 in diploid form is added to the germ cell or the cell, which is still in the '"retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
' ' '. . , C!!
. ;. C ' ' ';
i ';. i. ( i
- 16 À Microbiological method of reconstruction of the mouth According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added and, for specific reconstruction of the mouth, chromosome 22 in diploid form is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the organs in the chest According to the basic formula ((3)+1), chromosomes 13 and 21 (haploid) are added and, for specific reconstruction of the organs in the chest, chromosome 1 in diploid form is added to the germ cell or the cell which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the organs in the abdomen According to the basic formula ((3)+1), chromosomes 1 and 21 (haploid) are added and, for specific reconstruction of the organs in the abdomen, chromosome 13 in diploid form is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
À Microbiological method of reconstruction of the organs in the head According to the basic formula ((3)+1), chromosomes 1 and 13 (haploid) are added and, for specific reconstruction of the organs in the head, chromosome 21 in diploid form is added to the germ cell or the cell, which is still in the "retarded" prophase stage. The remaining procedure follows the description of Patent Claim 2.
i i. ; j . . . .. c;
PATENT CLAIM 1
Microbiological method for genetic reconstruction of human organs using endogenous cells and the genetic information contained therein.
First a cell is removed from a human organism for which a certain organ is to be cloned (in addition to somatic cells, germ cells are most suitable for this process). The cell thus removed is then placed into a culture medium and stimulated to mitosis by means of one of the methods currently available. An entire organism would be created by this method, but this isto be prevented in genetic reconstruction of individual organs, so the chromosomes that are not responsible for a certain organ are removed from the cell at the first cell division at the prophase time, when compression of the chromosome strands occurs and these become visible. The chromosomes to be removed are determined according to the internationally standardized karyogram (Convention of Denver 1960 - 22 autosomal pairs; 1 pair of heterochromosomes; in 7 groups according to size). Then the chromosomes required for the reconstruction according to the following formula: ((3)+1)
are added back to the cell. The three basic chromosomes are the autosomal pairs 1, 13 and 21. They must always be present only as haploid cells (with three exceptions, which are described below). The additional chromosome required, depending on the organ, is restored to either haploid or diploid form.
After this genetic engineering procedure, the cell is placed in a container with circulating culture medium and is stimulated to cell division with an input of energy according to traditional methods, which then leads to cell :: :..:. I.:.: I: r :i.. r . . i. , it,; C _ À C:
replication. During this process, a specific differentiation of the cells occurs, so that ultimately the form and function of the respective organ can develop.
À Microbiological method of reconstruction of the liver According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 17, 18, 19, 20 and 22 as well as the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 16, which is responsible for specific reconstruction of the liver, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the spleen According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 15, 16, 17, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 11, which is responsible for specific reconstruction of the spleen, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the gallbladder According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 14, 15, 16, 17, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 12, which is responsible for the specific reconstruction of the gallbladder, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
.; (,. , , i, (; r ( C.
À Microbiological method of reconstruction of the intestine According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5/ 6, 7, 8, 9, lOr 11, 12, 15, 16, 17, 18' lP' 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 14, which is responsible for specific reconstruction of the intestine, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right hand According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5r 6r 7r 9' 10, 11' 12, 14, 15, 16, 17, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 8 which is responsible for the specific reconstruction of the right hand, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the left hand According to the basic formula ((3)+1), chromosome pairs 2, 3; 4, 5' 6' 7, 8' 9, 11, 12; 14, 15; 16; 17; 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 10, which is responsible for the specific reconstruction of the left hand, also remains in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the left kidney According to the basic formula ((3)+1), chromosome pairs 2, C r;; i i. '...
C C i ' ( C C C., C C 2
C C C C C
- 20 3, 4, 5, 6, 7, 8, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 9, which is responsible for the specific reconstruction of the left kidney, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right kidney According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 6, which is responsible for the specific reconstruction of the right kidney, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right leg According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 5, which is responsible for specific reconstruction of the right leg, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
O Microbiological method of reconstruction of the left leg According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
. c i ,., c <. (; ( ( (
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 7, which is responsible for the specific reconstruction of the left leg, is also left in haploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
À Microbiological method of reconstruction of the right eye According to the basic formula ((3)+1), chromosome pairs 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 2, which is responsible for specific reconstruction of the right eye, is left in diploid form in the cell. The remaining procedure follows the description
of Patent Claim 1.
À Microbiological method of reconstruction of the left eye According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 19, which is responsible for the specific reconstruction of the left eye, is left in diploid form in the cell. The remaining procedure follows the description
of Patent Claim 1.
À Microbiological method of reconstruction of the right ear According to the basic formula ((3)+1), chromosome pairs 2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 3, which is responsible for specific reconstruction of the right ear, is left in diploid form in the cell. The remaining procedure follows the description
... ;..
c I ( '..
t. C; '. ' ' t 2.

Claims (2)

  1. - 22 of Patent Claim 1.
    À Microbiological method of reconstruction of the left ear According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 18, 19, 20 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
    Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 17, which is responsible for the specific reconstruction of the left ear, is left in diploid form in the cell. The remaining procedure follows the description
    of Patent Claim 1.
    À Microbiological method of reconstruction of the right half of the nose According to the basic formula ((3)+1), chromosome pairs 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
    and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
    Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 4, which Its responsible for the specific reconstruction of the right half of the nose, is left in diploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
    Microbiological method of reconstruction of the left half of the nose According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
    Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 20, which is responsible for the specific reconstruction of the left half of the nose, is left in diploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
    . ... . c c. <. c
    - 23 À Microbiological method of reconstruction of the mouth According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19 and 20 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
    Chromosomes 1, 13 and 21 are left in haploid form in the cell. Chromosome 22, which is responsible for specific reconstruction of the mouth, is left in diploid form in the cell. The remaining procedure follows the description of
    Patent Claim 1.
    À Microbiological method of reconstruction of the organs in the chest According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
    and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
    Chromosomes 13 and 21 are left in haploid form in the cell.
    Chromosome 1, which is responsible for the specific reconstruction of the chest, is left in diploid form in the cell. The remaining procedure follows the description of
    Patent Claim 1.
    À Microbiological method of reconstruction of the organs in the abdomen According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
    and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
    Chromosomes 1 and 21 are left in haploid form in the cell.
    Chromosome 13, which is responsible for the specific reconstruction of the organs in the abdomen, is left in diploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
    À Microbiological' method of reconstruction of the organs in the head . À. .... .
    c ".
    - 24 According to the basic formula ((3)+1), chromosome pairs 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20
    and 22 and the sex chromosomes are removed from the cell, which is still in the prophase stage.
    Chromosomes 1 and 13 are left in haploid form in the cell.
    Chromosome 21, which is responsible for the specific reconstruction of the organs in the head, is left in diploid form in the cell. The remaining procedure follows the description of Patent Claim 1.
    .. c. c... ..
    - 25 PATENT CLAIM 2
    Microbiological method of genetic reconstruction of human organs using endogenous cells and the genetic information contained therein.
    First, a cell is removed from a human organism for which a certain organ is to be cloned (in addition to somatic cells, germ cells are most suitable for this process). The cell thus removed is then placed in a culture medium and stimulated to mitosis by means of one of the methods currently available. According to this method, an entire organism would be created, and yet this is to be prevented in genetic reconstruction of individual organs, so the chromosomes that are not responsible for a certa n organ are removed from the cell at the first cell division at the prophase time, when compression of the chromosome strands occurs and these become visible. The chromosomes to be removed are determined according to the internationally standardized karyogram (Convention of Denver 1960 - 22 autosomal pairs; 1 pair of heterochromosomes; in 7 groups according to size).
    The formula for the microbiological method of genetic reconstruction of organs is given as follows: ((3)+1)
    The three basic chromosomes are the autosomal pairs 1, 13 and 21. They must always be present only as haploid cells (with three exceptions, which are described below). The additional chromosome required, depending on the organ, is left in either haploid or diploid form in the cell. After this genetic engineering procedure, the cell is placed in a container with circulating nutrient solution and is stimulated to cell division with an input of energy according to traditional methods, which then leads to cell (.-. .. c. À ( c (., c (.....
    replication. During this process, a specific differentiation of the cells occurs, so that ultimately the form and function of the respective organ can develop.
    À Microbiological method of reconstruction of the liver According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 16, also haploid, is also added for specific reconstruction of the liver. The remaining procedure follows the description of Patent Claim
    2. À Microbiological method of reconstruction of the spleen According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 11, also haploid, is also added for specific reconstruction of the spleen. The remaining procedure follows the description of Patent Claim
    2. À Microbiological method of reconstruction of the gallbladder According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 12, also haploid, is also added for specific reconstruction of the gallbladder.
    The remaining procedure follows the description of Patent
    Claim 2.
    Microbiological method of reconstruction of the intestine According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 14, also haploid, is also added for specific reconstruction of the intestine.
    The remaining procedure follows the description of Patent
    Claim 2.
    ,; ; c; At.
    À.
    - 27 À Microbiological method of reconstruction of the right hand According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 8, also haploid, is also added for specific reconstruction of the right hand. The remaining procedure follows the description of Patent Claim
    2. À Microbiological method of reconstruction of the left hand According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 10, also haploid, is also added for specific reconstruction of the left hand.
    The remaining procedure follows the description of Patent
    Claim 2.
    À Microbiological method of reconstruction of the left kidney According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 9, also haploid, is also added for specific reconstruction of the left kidney. The remaining procedure follows the description of Patent Claim
    2. À Microbiological method of reconstruction of the right kidney According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 6, also haploid, is also added for specific reconstruction of the right kidney. The remaining procedure follows the description of Patent Claim
    2. À Microbiological method of reconstruction of the right leg According to the basic formula ((3)+1), chromosomes 1, 13
    - 28 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 5, also haploid, is also added for specific reconstruction of the right leg. The remaining procedure follows the description of Patent Claim
    2. À Microbiological method of reconstruction of the left leg According to the basic formula ((3)+1), chromosomes l, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 7, also haplo d, is also added for specific reconstruction of the left '.eg. The remaining procedure follows the description of ?a-.ent Claim
    2. À Microbiological method of reconstruction of the right eye According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 2 in diploid form is also added for specific reconstruction of the r ght eye.
    The remaining procedure follows the description of Patent
    Claim 2.
    À Microbiological method of reconstruction of the left eye According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 19 in diploid form is also added for specific reconstruction of the left eye. The remaining procedure follows the description of Patent Claim
    2. À Microbiological method of reconstruction of the Light ear According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 3 in diploid form is also added for specific reconstruction of the right ear.
    The remaining procedure follows the description of Patent
    Claim 2.
    :: ( 1:: 1;, ...
    c, (.; c
    - 29 À Microbiological method of reconstruction of the left ear According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 17 in diploid form is also added for specific reconstruction of the left ear. The remaining procedure follows the description of Patent Claim
  2. 2. À Microbiological method of reconstruction of the organs in the chest According to the basic formula ((3)+1), chromosomes 13 and ... . . :. À. c
    - 30 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 1 in diploid form is also added for specific reconstruction of the organs in the chest. The remaining procedure follows the description of
    Patent Claim 2.
    À Microbiological method of reconstruction of the organs in the abdomen According to the basic formula ((3)+1), chromosomes 1 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 13 in diploid form is also added for specific reconstruction of the organs in the abdomen. The remaining procedure follows the description of
    Patent Claim 2.
    À Microbiological method of reconstruction of the organs in the head According to the basic formula ((3)+1), chromosomes 1 and 13 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 21 in diploid form is also added for specific reconstruction of the organs in the head. The remaining procedure follows the description of
    Patent Claim 2.
    . i ( ( c. .
    2. À Microbiological method of reconstruction of the right half of the nose According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 4 in diploid form is also added for specific reconstruction of the right half of the nose. The remaining procedure follows the description
    of Patent Claim 2.
    À Microbiological method of reconstruction of the left half of the nose According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 20 in diploid form is also added for specific reconstruction of the left half of the nose. The remaining procedure follows the description
    of Patent Claim 2.
    À Microbiological method of reconstruction of the mouth According to the basic formula ((3)+1), chromosomes 1, 13 and 21 (haploid) are added to the cell, which is still in the prophase stage, and chromosome 22 in diploid form is also added for specific reconstruction of the mouth. The remaining procedure follows the description of Patent Claim
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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999001163A1 (en) * 1997-07-03 1999-01-14 University Of Massachusetts Cloning using donor nuclei from non-serum starved, differentiated cells

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1195699A (en) * 1997-09-22 1998-10-14 任春严 Production of transplanting organ, and method and device for repairing organ and reproduction

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999001163A1 (en) * 1997-07-03 1999-01-14 University Of Massachusetts Cloning using donor nuclei from non-serum starved, differentiated cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WPI Abstract Accession No. 1999-096571 & CN 1195699 A *

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