WO2001092477A3 - Dreidimensionales hautmodell - Google Patents

Dreidimensionales hautmodell Download PDF

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Publication number
WO2001092477A3
WO2001092477A3 PCT/EP2001/006074 EP0106074W WO0192477A3 WO 2001092477 A3 WO2001092477 A3 WO 2001092477A3 EP 0106074 W EP0106074 W EP 0106074W WO 0192477 A3 WO0192477 A3 WO 0192477A3
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WO
WIPO (PCT)
Prior art keywords
vitro
methods
equivalent
dimensional
skin model
Prior art date
Application number
PCT/EP2001/006074
Other languages
English (en)
French (fr)
Other versions
WO2001092477A2 (de
Inventor
Michaela Noll
Thomas Graeve
Original Assignee
Fraunhofer Ges Forschung
Michaela Noll
Thomas Graeve
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10026789A external-priority patent/DE10026789B4/de
Priority claimed from DE10062623A external-priority patent/DE10062623B4/de
Priority to AU76348/01A priority Critical patent/AU7634801A/en
Priority to CA2410956A priority patent/CA2410956C/en
Priority to JP2002500671A priority patent/JP4751005B2/ja
Priority to DE50107916T priority patent/DE50107916D1/de
Application filed by Fraunhofer Ges Forschung, Michaela Noll, Thomas Graeve filed Critical Fraunhofer Ges Forschung
Priority to AT01953961T priority patent/ATE308610T1/de
Priority to US10/296,967 priority patent/US7553664B2/en
Priority to EP01953961A priority patent/EP1290145B1/de
Publication of WO2001092477A2 publication Critical patent/WO2001092477A2/de
Publication of WO2001092477A3 publication Critical patent/WO2001092477A3/de
Priority to US12/484,810 priority patent/US8222031B2/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3813—Epithelial cells, e.g. keratinocytes, urothelial cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
    • A61L27/24—Collagen
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A61L27/3852—Cartilage, e.g. meniscus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C12N5/0698—Skin equivalents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5082—Supracellular entities, e.g. tissue, organisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
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    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C12N2503/06—Screening or testing on artificial skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Dermatology (AREA)
  • Biotechnology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Vascular Medicine (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
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Abstract

Die vorliegende Erfindung betrifft Verfahren zur Kultivierung dermaler Fibroblasten, Verfahren zur Herstellung von in vitro-Dermisäquivalenten, Verfahren zur Herstellung von dreidimensionalen in vitro-Hautäquivalenten, ein in vitro-Dermisäquivalent, ein dreidimensionales in vitro-Hautäquivalent und Verfahren zur Bestimmung der Wirkung einer chemischen Substanz oder eines Agens auf menschliche Hautzellen unter Verwendung des in vitro-Dermisäquivalents und/oder des in vitro-Hautäquivalents.
PCT/EP2001/006074 2000-05-31 2001-05-29 Dreidimensionales hautmodell WO2001092477A2 (de)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP01953961A EP1290145B1 (de) 2000-05-31 2001-05-29 Dreidimensionales hautmodell
US10/296,967 US7553664B2 (en) 2000-05-31 2001-05-29 Three-dimensional skin model
CA2410956A CA2410956C (en) 2000-05-31 2001-05-29 Three-dimensional skin model
JP2002500671A JP4751005B2 (ja) 2000-05-31 2001-05-29 三次元皮膚モデル
DE50107916T DE50107916D1 (de) 2000-05-31 2001-05-29 Dreidimensionales hautmodell
AU76348/01A AU7634801A (en) 2000-05-31 2001-05-29 Three-dimensional skin model
AT01953961T ATE308610T1 (de) 2000-05-31 2001-05-29 Dreidimensionales hautmodell
US12/484,810 US8222031B2 (en) 2000-05-31 2009-06-15 Three-dimensional skin model

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10026789.O 2000-05-31
DE10026789A DE10026789B4 (de) 2000-05-31 2000-05-31 Knorpelersatz und Verfahren zu dessen Herstellung
DE10062623A DE10062623B4 (de) 2000-05-31 2000-12-15 Dreidimensionales Hautmodell
DE10062623.8 2000-12-15

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10296967 A-371-Of-International 2001-05-29
US12/484,810 Division US8222031B2 (en) 2000-05-31 2009-06-15 Three-dimensional skin model

Publications (2)

Publication Number Publication Date
WO2001092477A2 WO2001092477A2 (de) 2001-12-06
WO2001092477A3 true WO2001092477A3 (de) 2002-05-10

Family

ID=26005890

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006074 WO2001092477A2 (de) 2000-05-31 2001-05-29 Dreidimensionales hautmodell

Country Status (10)

Country Link
US (2) US7553664B2 (de)
EP (1) EP1290145B1 (de)
JP (1) JP4751005B2 (de)
AT (1) ATE308610T1 (de)
AU (1) AU7634801A (de)
CA (1) CA2410956C (de)
DE (1) DE50107916D1 (de)
DK (1) DK1290145T3 (de)
ES (1) ES2252259T3 (de)
WO (1) WO2001092477A2 (de)

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US7741116B2 (en) 2002-03-06 2010-06-22 University Of Cincinnati Surgical device for skin therapy or testing
DE102004039537A1 (de) * 2004-08-13 2006-02-23 Phenion Gmbh & Co. Kg Vernetzte Kollagenmatrix zur Herstellung eines Hautäquivalentes
US7247423B2 (en) * 2004-09-10 2007-07-24 Synergy Biosystems Ltd Method to measure the effect of topically applied agents using skin maintained in organ culture
DE102005028899B4 (de) * 2005-06-17 2007-09-20 Gkss-Forschungszentrum Geesthacht Gmbh Hautäquivalent und Verfahren zur Erzeugung desselben
US20070249044A1 (en) * 2006-01-30 2007-10-25 University Of Illinois At Chicago Microstructures in three dimensional gel suspensions for growth of cells
US8591933B2 (en) * 2007-07-18 2013-11-26 The Board Of Trustees Of The University Of Illinois Temporal release of growth factors from 3D micro rod scaffolds for tissue regeneration
KR101739330B1 (ko) * 2010-10-29 2017-05-24 (주)아모레퍼시픽 진피 모사체를 이용한 피부 탄력 증진력 평가법
DE102011121556B4 (de) * 2011-12-20 2015-12-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. In vitro 3D-Reporter-Hautmodell
US9878071B2 (en) * 2013-10-16 2018-01-30 Purdue Research Foundation Collagen compositions and methods of use
WO2015166455A1 (en) 2014-04-30 2015-11-05 Fondazione Istituto Italiano Di Tecnologia Method for producing a totally endogenous bioengineered tissue and tissue obtained thereby
DE102014214944A1 (de) 2014-07-30 2016-02-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zur Erzeugung reproduzierbarer Schnitte und/oder Ausnehmung in zumindest einer oberflächenelastischen Probe
EP3072535B1 (de) 2015-03-26 2017-03-01 Université de Bordeaux Verfahren zur rekonstruktion von haut
WO2016172365A1 (en) 2015-04-21 2016-10-27 Purdue Research Foundation Office Of Technology Commercialization Cell-collagen-silica composites and methods of making and using the same
US10839509B2 (en) 2015-07-10 2020-11-17 3Scan Inc. Spatial multiplexing of histological stains
KR20170055791A (ko) * 2015-11-12 2017-05-22 한국과학기술연구원 인 비트로 3ｄ 피부 진피 모델 및 그를 사용하여 약물을 스크리닝하는 방법
EP3615568A4 (de) 2017-04-25 2021-01-20 Purdue Research Foundation Dreidimensionaler (3d) gewebegezüchteter muskel zur geweberestauration
WO2019143498A1 (en) * 2018-01-22 2019-07-25 ClearIt, LLC Methods and compositions for simulation of the dermal compartment
FR3083246B1 (fr) * 2018-06-29 2023-12-08 Oreal Modeles cellulaires et tissulaires comprenant des fibroblastes de la jonction dermo-hypodermique et ses applications
US11911090B2 (en) 2018-12-19 2024-02-27 Clear Intradermal Technologies, Inc. Systems and methods for tattoo removal using an applied electric field
CN109602958B (zh) * 2019-01-11 2021-04-06 湖北中部医疗科技有限公司 一种人工皮肤及其制备方法
CA3205754A1 (en) 2021-01-22 2022-07-28 Biranchi Patra Topical human milk formulations
DE102021105932B4 (de) 2021-03-11 2023-02-16 Leica Biosystems Nussloch Gmbh Verfahren zur Gewebeprozessierung und Gewebeprozessor
CN113667632B (zh) * 2021-09-14 2023-10-17 上海艾济生物科技有限公司 细胞培养基添加剂、细胞培养基及细胞体外扩增的方法
GB202201229D0 (en) * 2022-01-31 2022-03-16 Alcyomics Ltd An innervated cellular composite
LU502391B1 (en) 2022-06-28 2024-01-09 Univerza V Mariboru A complex in vitro model of human skin, a process for preparation and use thereof

Citations (5)

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WO1991016010A1 (en) * 1990-04-24 1991-10-31 Mark Eisenberg Composite living skin equivalents
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AU7634801A (en) 2001-12-11
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JP4751005B2 (ja) 2011-08-17
ATE308610T1 (de) 2005-11-15
ES2252259T3 (es) 2006-05-16
EP1290145B1 (de) 2005-11-02
CA2410956A1 (en) 2001-12-06
US8222031B2 (en) 2012-07-17
US20040018149A1 (en) 2004-01-29
WO2001092477A2 (de) 2001-12-06
DK1290145T3 (da) 2006-03-13
US20090298042A1 (en) 2009-12-03
EP1290145A2 (de) 2003-03-12
JP2004505614A (ja) 2004-02-26
CA2410956C (en) 2012-12-04

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