WO2001091154A2 - Probenträger für massenspektrometer - Google Patents
Probenträger für massenspektrometer Download PDFInfo
- Publication number
- WO2001091154A2 WO2001091154A2 PCT/DE2001/002079 DE0102079W WO0191154A2 WO 2001091154 A2 WO2001091154 A2 WO 2001091154A2 DE 0102079 W DE0102079 W DE 0102079W WO 0191154 A2 WO0191154 A2 WO 0191154A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- biological samples
- sample
- sample carrier
- carrier according
- conductive material
- Prior art date
Links
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
Definitions
- the present invention describes' a sample holder for the mass spectrometer, especially for MALDI-TOF mass spectrometer.
- MALDI-TOF Matrix-assisted laser desorption / ionization time-of-flight mass spectrometry
- sample carriers are known in the prior art which are also suitable for MALDI-TOF mass spectrometry.
- US-A 4,384,193 describes a specimen slide device which is suitable for holding specimen slides and is used for incubating samples.
- MALDI-TOF mass spectrometry was not known at the time of publication of this publication.
- EP-A 0964427 describes holders for MALDI-TOF mass spectrometry in general.
- the supports for the analytes must always be made conductive. From DE-A 19712195 and ÜS-A 5770860 it is known that microtiter plates can also be used for MALDI-TOF mass spectrometry.
- sample carriers for MALDI-TOF mass spectrometry can also be made from hydrophobic material, but that care must always be taken to ensure that the surface becomes electrically conductive (for example through the incorporation of graphite).
- the metal sample carriers usually used for MALDI-TOF mass spectrometry which are designed for individual samples, often 26 or 100 samples or recently also for 384 samples, are usually of such a nature that the sample together with a matrix, usually an organic see acid, is applied directly to its position on the sample holder. They are essentially flat metal plates.
- the sample carriers with 384 sample positions recently sold by Bruker correspond in their grid and their dimensions to a microtiter plate. These sample carriers are not suitable for the direct use of biochips (oligomer arrays) in the mass spectrometer, since immobilization of oligonucleotides is required, which is only possible with considerable effort on a sample carrier.
- Specimen carriers used for immobilization on a surface have a metallic, glass-coated or chemically modified surface (WO 99/29898).
- the disadvantage of these surfaces is that the coating for highly sensitive analytics is not particularly uniform. Furthermore, the coating is usually either very labor-intensive and / or cost-intensive.
- Metal supports with a glass coating are known, but are more used to create a particularly smooth surface in order to solve critical calibration problems.
- biochips are often used in slide format.
- a few chemical modification steps are sufficient to activate the glass surface of a conventional slide, as is also used for microscopy, so that preferably a covalent chemical bond to applied probe oligonucleotides is possible, which lie in a predetermined grid on the surface of the slide ,
- the technical problem underlying the present invention was therefore that there is no technological compatibility in terms of material and shape for the use of biochips in the mass spectrometer.
- the object of the present invention is to provide a sample carrier which overcomes the disadvantages of the prior art.
- the invention relates to a sample carrier, which
- the object of the invention is achieved in that a sample carrier for mass spectrometers, in particular for MALDI-TOF mass spectrometers, consisting of metal or another conductive material is created, which is characterized in that this sample carrier has at least one recess for receiving carriers for biological samples having.
- the dimensions of the recesses correspond to commercially available specimen slides. It is particularly preferred that the dimensions are between 75 and 130 mm in length, between 25 and 100 mm in width and between 2 and 20 mm in height.
- a sample carrier according to the invention is preferred, at least one carrier for biological samples being attached to it. is arranged. It is preferred that the slides for biological samples correspond to commercially available slides. Furthermore, it is preferred that the dimensions of the carrier for biological samples 72-78 mm in length, 22 to '28 mm in width and 0.5 to 2 mm are located in the height. It is also preferred that the support for biological samples consists of metal.
- the support for biological samples consists of a non-conductive material and / or glass.
- DNA fragments or oligonucleotides in a rectangular or hexagonal pattern are applied to the support for biological samples.
- the attachment between the sample holder and the holder for biological samples is carried out by means of double-sided adhesive tape, screws, springs, snap locks, wire clips and / or rails.
- sample carrier according to the invention corresponds in format to a microtiter plate.
- a sample carrier with dimensions of approximately 123 mm ⁇ 82 mm ⁇ 9.5 mm is particularly preferred.
- Another object of the present invention is the use of a sample carrier according to one of the preceding claims for the analysis of polymorphisms and / or DNA methylation in DNA samples.
- the present invention thus describes a sample carrier for MALDI mass spectrometers.
- This sample holder consists of an electrically conductive material, preferred wise steel or aluminum.
- the invention is also based on the surprising finding that conventional specimen slides can be introduced into such a mass spectrometer without the lack of conductivity of the glass slide being significantly detrimental to the measurement result.
- the samples are on the plates that can be inserted in the sample holder.
- sample carriers on which supports for biological samples are applied or fastened which consist of non-metallic and / or non-conductive materials.
- Carriers for biological samples made of glass are very particularly preferred.
- a sample holder for a MALDI-TOF spectrometer preferably made of metal, which has cutouts for holding 1-5 plates, the dimensions of which preferably correspond to commercially available object holders.
- the dimensions of the sample carrier are preferably between 75 and 130 mm in length, between 25 and 100 mm in width and between 2 and 20 mm in height.
- the dimensions of the plates, which can be made of glass, silicon or metal, are particularly preferably 72-78 mm ⁇ 22-28 mm ⁇ 0.5-2 mm. This corresponds to the dimensions of commercial slides, the exact dimensions of which are variable even in international comparison.
- the plates are attached to the sample carrier with double-sided adhesive tape.
- fastening is carried out using screws, springs, snap locks, wire clips or rails.
- the sample carrier according to the invention thus combines the area of sample production and that of measuring the samples in an incredibly simple manner.
- the subject of the present invention and a particularly preferred embodiment is a sample carrier according to the invention for mass spectrometers, in particular for MALDI-TOF mass spectrometers, consisting of metal or another conductive material, this sample carrier having at least one recess for receiving carriers for biological samples and at least one arranged thereon
- This sample carrier having at least one recess for receiving carriers for biological samples and at least one arranged thereon
- Has supports for biological samples characterized in that the supports for biological samples consist of non-conductive material and / or glass. It is further preferred that the supports for biological samples consist of non-conductive material and / or glass
- the sample carrier is preferably used for the analysis of polymorphisms and / or DNA methylation in DNA samples.
- FIG. 1 shows a preferred embodiment of the sample holder according to the invention.
- the sample carrier P according to the invention made of metal or another conductive material for the MALDI-TOF device (BIFLEX III BT 1903, Bruker Analytical Systems) can preferably be loaded with 1 to 3 glass carriers G. These have a size of 76 mm x 25 mm x 1 mm.
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Electron Tubes For Measurement (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
Claims
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01949232A EP1284008B1 (de) | 2000-05-23 | 2001-05-23 | Probenträger für massenspektrometer |
DE50101992T DE50101992D1 (de) | 2000-05-23 | 2001-05-23 | Probenträger für massenspektrometer |
JP2001587458A JP2003534634A (ja) | 2000-05-23 | 2001-05-23 | 質量分析計の試料支持台 |
AT01949232T ATE264545T1 (de) | 2000-05-23 | 2001-05-23 | Probenträger für massenspektrometer |
AU2001270461A AU2001270461A1 (en) | 2000-05-23 | 2001-05-23 | Sample support for mass spectrometers |
CA002409840A CA2409840A1 (en) | 2000-05-23 | 2001-05-23 | Sample support for mass spectrometers |
US10/296,490 US6888131B2 (en) | 2000-05-23 | 2001-05-23 | Sample support for mass spectrometers |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10027120A DE10027120A1 (de) | 2000-05-23 | 2000-05-23 | Probenträger für Massenspektrometer |
DE10027120.0 | 2000-05-23 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2001091154A2 true WO2001091154A2 (de) | 2001-11-29 |
WO2001091154A3 WO2001091154A3 (de) | 2002-08-08 |
Family
ID=7644308
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DE2001/002079 WO2001091154A2 (de) | 2000-05-23 | 2001-05-23 | Probenträger für massenspektrometer |
Country Status (8)
Country | Link |
---|---|
US (1) | US6888131B2 (de) |
EP (1) | EP1284008B1 (de) |
JP (1) | JP2003534634A (de) |
AT (1) | ATE264545T1 (de) |
AU (1) | AU2001270461A1 (de) |
CA (1) | CA2409840A1 (de) |
DE (2) | DE10027120A1 (de) |
WO (1) | WO2001091154A2 (de) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2376465A (en) * | 2001-03-15 | 2002-12-18 | Bruker Daltonik Gmbh | Mass spectrometric analysis of multiple DNA chips |
JP2007508664A (ja) * | 2003-10-10 | 2007-04-05 | アプレラ コーポレイション | 取り外し可能な磁性挿入物を備えるmaldiプレート |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10027120A1 (de) * | 2000-05-23 | 2001-12-06 | Epigenomics Ag | Probenträger für Massenspektrometer |
WO2005070089A2 (en) * | 2004-01-13 | 2005-08-04 | Waters Investments Limited | Substrate adapter for use in mass spectroscopy analysis |
JP2007108015A (ja) * | 2005-10-13 | 2007-04-26 | Biologica:Kk | 分析用の保持体及びその利用 |
JP5020742B2 (ja) * | 2007-08-27 | 2012-09-05 | 日本電子株式会社 | Maldiイオン源を備えた質量分析装置およびmaldiイオン源用サンプルプレート |
WO2010095677A1 (ja) * | 2009-02-18 | 2010-08-26 | 日本電気株式会社 | ターゲットプレートおよびその製造方法 |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3096435A (en) * | 1960-12-09 | 1963-07-02 | Combustion Eng | Ion generating and focusing mechanism |
US3886358A (en) * | 1974-05-23 | 1975-05-27 | Us Energy | Specimen transfer container for ion microprobe mass analyzer |
GB2156073A (en) * | 1984-03-15 | 1985-10-02 | Prutec Ltd | Mass spectrometer sample introduction system |
GB2178534A (en) * | 1985-07-30 | 1987-02-11 | Univ Pasteur | Device and process for the direct introduction of samples into a mass spectrometer |
US5498545A (en) * | 1994-07-21 | 1996-03-12 | Vestal; Marvin L. | Mass spectrometer system and method for matrix-assisted laser desorption measurements |
US5770860A (en) * | 1996-07-12 | 1998-06-23 | Franzen; Jochen | Method for loading sample supports for mass spectrometers |
WO1999000657A1 (en) * | 1997-06-26 | 1999-01-07 | Perseptive Biosystems, Inc. | High density sample holder for analysis of biological samples |
WO2000067293A1 (en) * | 1999-04-29 | 2000-11-09 | Ciphergen Biosystems, Inc. | Sample holder with hydrophobic coating for gas phase mass spectrometers |
WO2001090761A2 (en) * | 2000-05-19 | 2001-11-29 | Imago Scientific Instruments | Methods of sampling specimens for microanalysis |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4384193A (en) * | 1981-06-09 | 1983-05-17 | Immulok, Inc. | Incubating device for specimen mounted on glass slides in immunoassays |
US5605798A (en) * | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
KR19990064358A (ko) * | 1995-10-18 | 1999-07-26 | 다윈 몰레큘러 코포레이션 | 하이브리드화용 고체 지지체의 제조 방법 및 비-특이적 배경의 감소 방법 |
DE19712195B4 (de) * | 1997-03-22 | 2007-12-27 | Friedrich-Schiller-Universität Jena | Verfahren und Vorrichtung zur Bereitstellung von Proben für den off-line Nachweis von Analyten nach der MALDI-Massenspektrometrie |
US6175112B1 (en) * | 1998-05-22 | 2001-01-16 | Northeastern University | On-line liquid sample deposition interface for matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectroscopy |
WO1999029898A2 (de) * | 1997-12-05 | 1999-06-17 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zur identifikation von nucleinsäuren durch matrix-assistierte laser desorptions/ionisations massenspektrometrie |
US6849847B1 (en) * | 1998-06-12 | 2005-02-01 | Agilent Technologies, Inc. | Ambient pressure matrix-assisted laser desorption ionization (MALDI) apparatus and method of analysis |
DE19834070B4 (de) * | 1998-07-29 | 2007-02-15 | Bruker Daltonik Gmbh | Ionisierung hochmolekularer Substanzen durch Laserdesorption aus flüssigen Matrices |
DE10027120A1 (de) * | 2000-05-23 | 2001-12-06 | Epigenomics Ag | Probenträger für Massenspektrometer |
CA2425476C (en) * | 2000-10-10 | 2011-02-01 | Biotrove, Inc. | Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof |
-
2000
- 2000-05-23 DE DE10027120A patent/DE10027120A1/de not_active Withdrawn
-
2001
- 2001-05-23 WO PCT/DE2001/002079 patent/WO2001091154A2/de active IP Right Grant
- 2001-05-23 CA CA002409840A patent/CA2409840A1/en not_active Abandoned
- 2001-05-23 AU AU2001270461A patent/AU2001270461A1/en not_active Abandoned
- 2001-05-23 AT AT01949232T patent/ATE264545T1/de not_active IP Right Cessation
- 2001-05-23 US US10/296,490 patent/US6888131B2/en not_active Expired - Fee Related
- 2001-05-23 JP JP2001587458A patent/JP2003534634A/ja active Pending
- 2001-05-23 DE DE50101992T patent/DE50101992D1/de not_active Expired - Fee Related
- 2001-05-23 EP EP01949232A patent/EP1284008B1/de not_active Expired - Lifetime
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3096435A (en) * | 1960-12-09 | 1963-07-02 | Combustion Eng | Ion generating and focusing mechanism |
US3886358A (en) * | 1974-05-23 | 1975-05-27 | Us Energy | Specimen transfer container for ion microprobe mass analyzer |
GB2156073A (en) * | 1984-03-15 | 1985-10-02 | Prutec Ltd | Mass spectrometer sample introduction system |
GB2178534A (en) * | 1985-07-30 | 1987-02-11 | Univ Pasteur | Device and process for the direct introduction of samples into a mass spectrometer |
US5498545A (en) * | 1994-07-21 | 1996-03-12 | Vestal; Marvin L. | Mass spectrometer system and method for matrix-assisted laser desorption measurements |
US5770860A (en) * | 1996-07-12 | 1998-06-23 | Franzen; Jochen | Method for loading sample supports for mass spectrometers |
WO1999000657A1 (en) * | 1997-06-26 | 1999-01-07 | Perseptive Biosystems, Inc. | High density sample holder for analysis of biological samples |
WO2000067293A1 (en) * | 1999-04-29 | 2000-11-09 | Ciphergen Biosystems, Inc. | Sample holder with hydrophobic coating for gas phase mass spectrometers |
WO2001090761A2 (en) * | 2000-05-19 | 2001-11-29 | Imago Scientific Instruments | Methods of sampling specimens for microanalysis |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2376465A (en) * | 2001-03-15 | 2002-12-18 | Bruker Daltonik Gmbh | Mass spectrometric analysis of multiple DNA chips |
GB2376465B (en) * | 2001-03-15 | 2005-03-02 | Bruker Daltonik Gmbh | Mass spectrometric genotyping |
JP2007508664A (ja) * | 2003-10-10 | 2007-04-05 | アプレラ コーポレイション | 取り外し可能な磁性挿入物を備えるmaldiプレート |
Also Published As
Publication number | Publication date |
---|---|
DE50101992D1 (de) | 2004-05-19 |
WO2001091154A3 (de) | 2002-08-08 |
EP1284008A2 (de) | 2003-02-19 |
ATE264545T1 (de) | 2004-04-15 |
AU2001270461A1 (en) | 2001-12-03 |
US6888131B2 (en) | 2005-05-03 |
DE10027120A1 (de) | 2001-12-06 |
CA2409840A1 (en) | 2002-11-22 |
EP1284008B1 (de) | 2004-04-14 |
JP2003534634A (ja) | 2003-11-18 |
US20040113066A1 (en) | 2004-06-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1618592B1 (de) | Massenspektrometer mit einer probenplatte für matrix-unterstützte laserdesorption und ionisation | |
DE10140499B4 (de) | Probenträgerplatten für Massenspektrometrie mit Ionisierung durch matrixunterstützte Laserdesorption | |
Jespersen et al. | Attomole detection of proteins by matrix‐assisted laser desorption/ionization mass spectrometry with the use of picolitre vials | |
US6287872B1 (en) | Sample support plates for Maldi mass spectrometry including methods for manufacture of plates and application of sample | |
EP1166328B1 (de) | Gaseinlass zur erzeugung eines gerichteten und gekühlten gasstrahls | |
US7030373B2 (en) | MALDI plate construction with grid | |
DE112015002731B4 (de) | Zweidimensionale MS/MS-Erfassungsmodi | |
US20110056311A1 (en) | Method of Scanning a Sample Plate Surface Mask in an Area Adjacent to a Conductive Area Using Matrix-Assisted Laser Desorption and Ionization Mass Spectrometry | |
WO2001091154A2 (de) | Probenträger für massenspektrometer | |
DE69418063T2 (de) | Zykloidisches massenspektrometer | |
EP3686588A1 (de) | Laser-desorptions-/ionisationsverfahren und massenspektrometrieverfahren | |
DE19834070B4 (de) | Ionisierung hochmolekularer Substanzen durch Laserdesorption aus flüssigen Matrices | |
CN111051874A (zh) | 激光解吸电离法、质量分析方法、试样支撑体及试样支撑体的制造方法 | |
DE10112387B4 (de) | Massenspektrometrische Genotypisierung | |
EP2808887A1 (de) | Messplatte für MALDI-Massenspektrometrie | |
DE112005000150B4 (de) | Substratadapter für die Verwendung bei der massenspektrometrischen Analyse | |
EP1573776B1 (de) | Verfahren zur herstellung eines probenträgers für die maldi-massenspektrometrie | |
DE19543065C2 (de) | Verfahren zur Genomanalyse und Mittel zur Durchführung des Verfahrens | |
EP4137448A1 (de) | Probenträger | |
DE102020113580A1 (de) | Verbesserte Elektrodenanordnung | |
EP1846165B1 (de) | Maldi-chiphalter | |
DD286667A5 (de) | Glimmentladungslampe zur schichtanalyse nichtebener flaechen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
AK | Designated states |
Kind code of ref document: A3 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A3 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2001949232 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2409840 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 10296490 Country of ref document: US Ref document number: 2001270461 Country of ref document: AU |
|
WWP | Wipo information: published in national office |
Ref document number: 2001949232 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 2001949232 Country of ref document: EP |