WO2001055353A3 - Recombinant attenuation of prrsv - Google Patents

Recombinant attenuation of prrsv Download PDF

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Publication number
WO2001055353A3
WO2001055353A3 PCT/EP2001/000865 EP0100865W WO0155353A3 WO 2001055353 A3 WO2001055353 A3 WO 2001055353A3 EP 0100865 W EP0100865 W EP 0100865W WO 0155353 A3 WO0155353 A3 WO 0155353A3
Authority
WO
WIPO (PCT)
Prior art keywords
orf
viruses
prrsv
prrs
attenuation
Prior art date
Application number
PCT/EP2001/000865
Other languages
French (fr)
Other versions
WO2001055353A2 (en
Inventor
Knut Elbers
Stefan Pesch
Heike Dreier
Original Assignee
Boehringer Ingelheim Vetmed
Knut Elbers
Stefan Pesch
Heike Dreier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE2000103373 external-priority patent/DE10003373A1/en
Priority claimed from DE2000103371 external-priority patent/DE10003371A1/en
Priority claimed from DE2000103372 external-priority patent/DE10003372A1/en
Priority to AU2001228502A priority Critical patent/AU2001228502A1/en
Priority to JP2001554384A priority patent/JP2003520601A/en
Priority to EP01946891A priority patent/EP1254213A2/en
Application filed by Boehringer Ingelheim Vetmed, Knut Elbers, Stefan Pesch, Heike Dreier filed Critical Boehringer Ingelheim Vetmed
Priority to CA002396454A priority patent/CA2396454A1/en
Priority to SK1093-2002A priority patent/SK10932002A3/en
Priority to MXPA02007198A priority patent/MXPA02007198A/en
Priority to EA200200743A priority patent/EA200200743A1/en
Priority to HU0204176A priority patent/HUP0204176A3/en
Priority to BR0107827-5A priority patent/BR0107827A/en
Publication of WO2001055353A2 publication Critical patent/WO2001055353A2/en
Publication of WO2001055353A3 publication Critical patent/WO2001055353A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/04—Inactivation or attenuation; Producing viral sub-units
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/522—Bacterial cells; Fungal cells; Protozoal cells avirulent or attenuated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5254—Virus avirulent or attenuated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/10011—Arteriviridae
    • C12N2770/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/10011—Arteriviridae
    • C12N2770/10061—Methods of inactivation or attenuation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Surface Acoustic Wave Elements And Circuit Networks Thereof (AREA)

Abstract

The present invention relates to live PRRS viruses which are attenuated by amino acid mutations in a specific site of the viral protein coded by the open reading frame (ORF) selected from the group of ORF 1a, ORF 1b and/or ORF 2. The invention also pertains to nucleotide sequences coding said viruses, methods of generating such viruses and their use for the preparation of a pharmaceutical composition for the prophylaxis and treatment of PRRS infections.
PCT/EP2001/000865 2000-01-26 2001-01-26 Recombinant attenuation of prrsv WO2001055353A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
BR0107827-5A BR0107827A (en) 2000-01-26 2001-01-26 Recombinant attenuation of prrsv
HU0204176A HUP0204176A3 (en) 2000-01-26 2001-01-26 Recombinant attenuation of prrsv
EA200200743A EA200200743A1 (en) 2000-01-26 2001-01-26 RECOMBINANT REDUCTION OF THE VIRUS REPRODUCTIVE AND RESPIRATORY SYNDROME OF PIGS (PRRSV)
JP2001554384A JP2003520601A (en) 2000-01-26 2001-01-26 Recombinant attenuation of PRRSV
EP01946891A EP1254213A2 (en) 2000-01-26 2001-01-26 Recombinant attenuation of prrsv
AU2001228502A AU2001228502A1 (en) 2000-01-26 2001-01-26 Recombinant attenuation of prrsv
CA002396454A CA2396454A1 (en) 2000-01-26 2001-01-26 Recombinant attenuation of prrsv
SK1093-2002A SK10932002A3 (en) 2000-01-26 2001-01-26 Live attenuated prrs virus containing orf 1a, 1b and 2, a nucleotide sequence coding said virus, method of generating such virus, pharmaceutical composition comprising same and use thereof
MXPA02007198A MXPA02007198A (en) 2000-01-26 2001-01-26 Recombinant attenuation of prrsv.

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE2000103373 DE10003373A1 (en) 2000-01-26 2000-01-26 Novel live porcine reproductive and respiratory syndrome virus attenuated by mutations in specific site of viral protein coded by specified open reading frames, useful for prophylaxis/treatment of viral infections
DE10003371.7 2000-01-26
DE10003372.5 2000-01-26
DE2000103372 DE10003372A1 (en) 2000-01-26 2000-01-26 Novel live porcine reproductive and respiratory syndrome virus attenuated by mutations in specific site of viral protein coded by specified open reading frames, useful for prophylaxis/treatment of viral infections
DE10003373.3 2000-01-26
DE2000103371 DE10003371A1 (en) 2000-01-26 2000-01-26 Novel live porcine reproductive and respiratory syndrome virus attenuated by mutations in specific site of viral protein coded by specified open reading frames, useful for prophylaxis/treatment of viral infections

Publications (2)

Publication Number Publication Date
WO2001055353A2 WO2001055353A2 (en) 2001-08-02
WO2001055353A3 true WO2001055353A3 (en) 2001-12-27

Family

ID=27213605

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/000865 WO2001055353A2 (en) 2000-01-26 2001-01-26 Recombinant attenuation of prrsv

Country Status (15)

Country Link
EP (1) EP1254213A2 (en)
JP (1) JP2003520601A (en)
KR (1) KR20030032910A (en)
CN (1) CN1406277A (en)
AR (1) AR027291A1 (en)
AU (1) AU2001228502A1 (en)
BR (1) BR0107827A (en)
CA (1) CA2396454A1 (en)
CZ (1) CZ20022554A3 (en)
EA (1) EA200200743A1 (en)
HU (1) HUP0204176A3 (en)
MX (1) MXPA02007198A (en)
PL (1) PL356274A1 (en)
SK (1) SK10932002A3 (en)
WO (1) WO2001055353A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0207988A (en) * 2001-03-09 2004-10-26 Boehringer Ingelheim Vetmed Live attenuated strains of prrs virus
US6841364B2 (en) * 2002-01-22 2005-01-11 Protatek International, Inc. Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and expression vectors thereof
MXPA05010260A (en) 2003-03-27 2006-03-17 Ottawa Health Research Inst Mutant vesicular stomatitis viruses and use thereof.
KR100996105B1 (en) * 2005-02-25 2010-11-22 화이자 프로덕츠 인크. N Protein Mutants of Porcine Reproductive and Respiratory Syndrome Virus
CN100439494C (en) * 2007-03-14 2008-12-03 中国动物疫病预防控制中心 Swine reproduction and respiration syndrome vaccine and its prepn process and application
US7666585B2 (en) 2007-06-15 2010-02-23 Protatek International, Inc. Construction of chimera PRRSV, compositions and vaccine preparations
CN101307305B (en) * 2008-04-30 2010-06-16 中国动物疫病预防控制中心 Low virulent strain of porcine reproductive and respiratory syndrome virus, immunogenicity immunogenicity material and vaccine
UA108902C2 (en) * 2010-11-10 2015-06-25 NORTH AMERICAN REPRODUCTIVE AND RESPIRATORY PIG SYNDROME (PRRS) VIRUS
MX2016012276A (en) * 2014-03-21 2017-06-12 Nutech Ventures A non-naturally occuring porcine reproductive and respiratory syndrome virus (prrsv) and methods of using.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996004010A1 (en) * 1994-08-05 1996-02-15 Regents Of The University Of Minnesota Vr-2332 viral nucleotide sequence and methods of use
WO1996040932A1 (en) * 1995-06-07 1996-12-19 Paul Prem S Vaccine for porcine reproductive and respiratory syndrome virus
EP0839912A1 (en) * 1996-10-30 1998-05-06 Instituut Voor Dierhouderij En Diergezondheid (Id-Dlo) Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996004010A1 (en) * 1994-08-05 1996-02-15 Regents Of The University Of Minnesota Vr-2332 viral nucleotide sequence and methods of use
WO1996040932A1 (en) * 1995-06-07 1996-12-19 Paul Prem S Vaccine for porcine reproductive and respiratory syndrome virus
EP0839912A1 (en) * 1996-10-30 1998-05-06 Instituut Voor Dierhouderij En Diergezondheid (Id-Dlo) Infectious clones of RNA viruses and vaccines and diagnostic assays derived thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALLENDE R. ET AL.: "Mutations in the genome of porcine reproductive and respiratory syndrome virus responsible for the attenuation phenotype", ARCHIVES OF VIROLOGY, vol. 145, no. 6, June 2000 (2000-06-01), VIENNA AT, pages 1149 - 1161, XP000926571 *
SNIJDER E J ET AL: "THE MOLECULAR BIOLOGY OF ARTERIVIRUSES", JOURNAL OF GENERAL VIROLOGY READING, vol. 79, 1998, GB, pages 961 - 979, XP000791110, ISSN: 0022-1317 *
YANG S X ET AL: "Comparative sequence analysis of open reading frames 2 to 7 of the modified live vaccine virus and other North American isolates of the porcine reproductive and respiratory syndrome virus.", ARCHIVES OF VIROLOGY, vol. 143, no. 3, 1998, pages 601 - 612, XP000926565, ISSN: 0304-8608 *
YUAN SHISHAN ET AL: "Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains.", VIRUS RESEARCH, vol. 74, no. 1-2, April 2001 (2001-04-01), pages 99 - 110, XP000926563, ISSN: 0168-1702 *

Also Published As

Publication number Publication date
CA2396454A1 (en) 2001-08-02
KR20030032910A (en) 2003-04-26
SK10932002A3 (en) 2002-11-06
AU2001228502A1 (en) 2001-08-07
AR027291A1 (en) 2003-03-19
CZ20022554A3 (en) 2002-10-16
WO2001055353A2 (en) 2001-08-02
EP1254213A2 (en) 2002-11-06
HUP0204176A2 (en) 2003-04-28
MXPA02007198A (en) 2002-12-09
HUP0204176A3 (en) 2003-12-29
CN1406277A (en) 2003-03-26
JP2003520601A (en) 2003-07-08
BR0107827A (en) 2002-11-05
EA200200743A1 (en) 2002-12-26
PL356274A1 (en) 2004-06-28

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