WO2001020016A3 - Procede d'augmentation du rendement de proteines de recombinaison dans des processus de fermentation microbiens - Google Patents

Procede d'augmentation du rendement de proteines de recombinaison dans des processus de fermentation microbiens Download PDF

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Publication number
WO2001020016A3
WO2001020016A3 PCT/EP2000/008984 EP0008984W WO0120016A3 WO 2001020016 A3 WO2001020016 A3 WO 2001020016A3 EP 0008984 W EP0008984 W EP 0008984W WO 0120016 A3 WO0120016 A3 WO 0120016A3
Authority
WO
WIPO (PCT)
Prior art keywords
yield
recombinant proteins
increasing
carbon
microbial fermentation
Prior art date
Application number
PCT/EP2000/008984
Other languages
German (de)
English (en)
Other versions
WO2001020016A2 (fr
Inventor
Peter Neubauer
Hong Ying Lin
Original Assignee
Univ Halle Wittenberg
Peter Neubauer
Hong Ying Lin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Halle Wittenberg, Peter Neubauer, Hong Ying Lin filed Critical Univ Halle Wittenberg
Priority to KR1020027003256A priority Critical patent/KR20020048934A/ko
Priority to JP2001523787A priority patent/JP2003530823A/ja
Priority to CA002383831A priority patent/CA2383831A1/fr
Priority to IL14857500A priority patent/IL148575A0/xx
Priority to EP00967674A priority patent/EP1212450A2/fr
Priority to AU77763/00A priority patent/AU775301B2/en
Priority to NZ517547A priority patent/NZ517547A/en
Publication of WO2001020016A2 publication Critical patent/WO2001020016A2/fr
Publication of WO2001020016A3 publication Critical patent/WO2001020016A3/fr
Priority to HK03104274A priority patent/HK1052029A1/xx

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Lors de la production de protéines de recombinaison dans des fermentations à écoulement discontinu, les procédés connus entraînent souvent, après induction de la synthèse de produits de recombinaison, une croissance trop forte de la culture due aux cellules exemptes de plasmides et une réduction du rendement du produit spécifique. Selon la présente invention, le rendement de protéines de recombinaison est augmenté du fait que la concentration de la source d'énergie/de carbone dans la culture est augmentée ou diminuée constamment sur un court laps de temps. Les oscillations sont produites par la modification de la cadence d'addition des solutions nutritives contenant la source d'énergie/de carbone. Ledit procédé est approprié pour tous les micro-organismes qui sont cultivés par écoulement discontinu avec limitation du carbone.
PCT/EP2000/008984 1999-09-14 2000-09-13 Procede d'augmentation du rendement de proteines de recombinaison dans des processus de fermentation microbiens WO2001020016A2 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
KR1020027003256A KR20020048934A (ko) 1999-09-14 2000-09-13 미생물 발효 공정에서 재조합 단백질의 수율을 증진시키는방법
JP2001523787A JP2003530823A (ja) 1999-09-14 2000-09-13 微生物発酵工程において組換え蛋白質の収率を高めるための方法
CA002383831A CA2383831A1 (fr) 1999-09-14 2000-09-13 Procede d'augmentation du rendement de proteines de recombinaison dans des processus de fermentation microbiens
IL14857500A IL148575A0 (en) 1999-09-14 2000-09-13 Method for increasing the yield of recombinant proteins in microbial fermentation processes
EP00967674A EP1212450A2 (fr) 1999-09-14 2000-09-13 Procede d'augmentation du rendement de proteines de recombinaison dans des processus de fermentation microbiens
AU77763/00A AU775301B2 (en) 1999-09-14 2000-09-13 Method for increasing the yield of recombinant proteins in microbial fermentation processes
NZ517547A NZ517547A (en) 1999-09-14 2000-09-13 Method for increasing the yield of recombinant proteins in microbial fermentation processes
HK03104274A HK1052029A1 (en) 1999-09-14 2003-06-16 Method for increasing the yield of recombinant proteins in microbial fermentation processes.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19943919A DE19943919B4 (de) 1999-09-14 1999-09-14 Verfahren zur Steigerung der Ausbeute von rekombinanten Proteinen in mikrobiellen Fermentationsprozessen
DE19943919.2 1999-09-14

Publications (2)

Publication Number Publication Date
WO2001020016A2 WO2001020016A2 (fr) 2001-03-22
WO2001020016A3 true WO2001020016A3 (fr) 2001-05-17

Family

ID=7921925

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/008984 WO2001020016A2 (fr) 1999-09-14 2000-09-13 Procede d'augmentation du rendement de proteines de recombinaison dans des processus de fermentation microbiens

Country Status (11)

Country Link
EP (1) EP1212450A2 (fr)
JP (1) JP2003530823A (fr)
KR (1) KR20020048934A (fr)
CN (1) CN1175113C (fr)
AU (1) AU775301B2 (fr)
CA (1) CA2383831A1 (fr)
DE (1) DE19943919B4 (fr)
HK (1) HK1052029A1 (fr)
IL (1) IL148575A0 (fr)
NZ (1) NZ517547A (fr)
WO (1) WO2001020016A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10011728B4 (de) * 2000-03-10 2005-02-10 Andreas Schmid Verfahren zur Leistungssteigerung mikrobieller Systeme
AU3944002A (en) * 2000-11-03 2002-05-27 Genentech Inc Metabolic rate shifts in fermentations expressing recombinant proteins
WO2002059233A2 (fr) 2000-11-17 2002-08-01 Microbes, Inc. Craquage modulable d'alcanes normaux et ramifies dans des huiles induit par des bacteries
CN1304560C (zh) * 2001-10-01 2007-03-14 诺维信公司 采用循环脉冲－暂停补料方式的发酵法
EP1885847B1 (fr) * 2005-05-26 2011-07-06 Cytos Biotechnology AG Procédé de fermentation extensible
FI20065762A0 (fi) * 2006-11-30 2006-11-30 Oulun Yliopisto Menetelmä soluviljelmän kasvun kontrolloimiseksi
CN106222152A (zh) * 2016-08-09 2016-12-14 苏州开元民生科技股份有限公司 一种产(‑)γ‑内酰胺酶重组大肠杆菌的发酵方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0397097A1 (fr) * 1989-05-08 1990-11-14 Research Association For Utilization Of Light Oil Culture de micro-organismes transformés

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0397097A1 (fr) * 1989-05-08 1990-11-14 Research Association For Utilization Of Light Oil Culture de micro-organismes transformés

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
IMPOOLSUP A ET AL: "STABILIZATION OF A RECOMBINANT YEAST PLASMID IN NON-SELECTIVE MEDIUM BY CYCLIC GROWTH RATE CHANGES", BIOTECHNOLOGY LETTERS, vol. 11, no. 9, 1989, pages 605 - 608, XP000982590, ISSN: 0141-5492 *
KERNS G ET AL: "OSCILLATING-FED-BATCH-TECHNIQUE IN OBTAINING CELLULASE", ACTA BIOTECHNOLOGICA, vol. 8, no. 3, 1988, pages 285 - 289, XP000982457, ISSN: 0138-4988 *
NEUBAUER P ET AL: "Response of guanosine tetraphosphate to glucose fluctuations in fed-batch cultivations of Escherichia coli", JOURNAL OF BIOTECHNOLOGY,NL,ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, vol. 43, no. 3, 15 December 1995 (1995-12-15), pages 195 - 204, XP004036875, ISSN: 0168-1656 *
TORNKVIST M ET AL: "Protein release and foaming in Escherichia coli cultures grown in minimal medium.", BIOPROCESS ENGINEERING, vol. 15, no. 5, 1996, pages 231 - 237, XP000982521, ISSN: 0178-515X *
YAZDANI SYED SHAMS ET AL: "Overexpression of streptokinase using a fed-batch strategy.", BIOTECHNOLOGY LETTERS, vol. 20, no. 10, October 1998 (1998-10-01), pages 923 - 927, XP000982533, ISSN: 0141-5492 *
YING LIN H ET AL: "Influence of controlled glucose oscillations on a fed-batch process of recombinant Escherichia coli", BRAUWELT,NUERNBERG,DE, vol. 79, no. 1, 14 April 2000 (2000-04-14), pages 27 - 37, XP004222563, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
CN1175113C (zh) 2004-11-10
AU7776300A (en) 2001-04-17
DE19943919A1 (de) 2001-03-22
CN1391614A (zh) 2003-01-15
EP1212450A2 (fr) 2002-06-12
JP2003530823A (ja) 2003-10-21
IL148575A0 (en) 2002-09-12
CA2383831A1 (fr) 2001-03-22
WO2001020016A2 (fr) 2001-03-22
DE19943919B4 (de) 2004-05-27
AU775301B2 (en) 2004-07-29
KR20020048934A (ko) 2002-06-24
NZ517547A (en) 2004-03-26
HK1052029A1 (en) 2003-08-29

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