WO2001018233A3 - Screening for inhibitors of lumazine synthase - Google Patents

Screening for inhibitors of lumazine synthase Download PDF

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Publication number
WO2001018233A3
WO2001018233A3 PCT/EP2000/007796 EP0007796W WO0118233A3 WO 2001018233 A3 WO2001018233 A3 WO 2001018233A3 EP 0007796 W EP0007796 W EP 0007796W WO 0118233 A3 WO0118233 A3 WO 0118233A3
Authority
WO
WIPO (PCT)
Prior art keywords
dimethyl
ribityllumazine
screening
level
mixture
Prior art date
Application number
PCT/EP2000/007796
Other languages
French (fr)
Other versions
WO2001018233A9 (en
WO2001018233A2 (en
Inventor
Adelbert Bacher
Simone Moertl
Original Assignee
Adelbert Bacher
Simone Moertl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Adelbert Bacher, Simone Moertl filed Critical Adelbert Bacher
Priority to EP00960438A priority Critical patent/EP1208221A2/en
Priority to AU72748/00A priority patent/AU7274800A/en
Publication of WO2001018233A2 publication Critical patent/WO2001018233A2/en
Publication of WO2001018233A3 publication Critical patent/WO2001018233A3/en
Publication of WO2001018233A9 publication Critical patent/WO2001018233A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method is described for screening for the presence or absence of inhibition of 6,7-dimethyl-8-ribityllumazine synthase activity comprising the following steps : (a) preparing a first aqueous mixture containing a protein having a plant-type 6,7-dimethyl-8-ribityllumazine synthase sequence, 5-amino-6-ribitylamino-2,4(1H,3H)pyrimidine-dione and 3,4-dihydroxy-2-butanone 4-phosphate; (b) reacting said first mixture during a predetermined period of time at a predetermined temperature and subsequently detecting the level of 6,7-dimethyl-8-ribityllumazine; (c) preparing a second aqueous mixture by including in said first mixture a predetermined amount of a chemical test sample; (d) reacting said second mixture during the predetermined period of time at the predetermined temperature and subsequently detecting the level of 6,7-dimethyl-8-ribityllumazine; (e) determining the presence of inhibition of 6,7-dimethyl-8-ribityllumazine synthase by observation of whether the level detected in step (d) is lower than the level detected in step (b).
PCT/EP2000/007796 1999-09-03 2000-08-10 Screening for inhibitors of lumazine synthase WO2001018233A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP00960438A EP1208221A2 (en) 1999-09-03 2000-08-10 Screening for inhibitors of lumazine synthase
AU72748/00A AU7274800A (en) 1999-09-03 2000-08-10 Method for screening for inhibitors of the biosynthesis of riboflavin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19942175.7 1999-09-03
DE1999142175 DE19942175A1 (en) 1999-09-03 1999-09-03 Method for finding inhibitors of riboflavin biosynthesis

Publications (3)

Publication Number Publication Date
WO2001018233A2 WO2001018233A2 (en) 2001-03-15
WO2001018233A3 true WO2001018233A3 (en) 2001-05-17
WO2001018233A9 WO2001018233A9 (en) 2002-09-06

Family

ID=7920749

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/007796 WO2001018233A2 (en) 1999-09-03 2000-08-10 Screening for inhibitors of lumazine synthase

Country Status (4)

Country Link
EP (1) EP1208221A2 (en)
AU (1) AU7274800A (en)
DE (1) DE19942175A1 (en)
WO (1) WO2001018233A2 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999038986A2 (en) * 1998-01-30 1999-08-05 Novartis Ag Riboflavin biosynthesis genes from plants and uses thereof
EP1010760A1 (en) * 1998-11-11 2000-06-21 E.I. Du Pont De Nemours And Company Lumazine synthase and riboflavin synthase
WO2000040744A1 (en) * 1998-12-15 2000-07-13 Adelbert Bacher Method for screening for inhibitors of riboflavin biosynthesis
WO2000044727A1 (en) * 1999-01-30 2000-08-03 Adelbert Bacher Inhibitors for the biosynthesis of vitamin b2 and method for producing same
US6146866A (en) * 1997-08-15 2000-11-14 E.I. Du Pont De Nemours And Company Lumazine synthase and riboflavin synthase

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6146866A (en) * 1997-08-15 2000-11-14 E.I. Du Pont De Nemours And Company Lumazine synthase and riboflavin synthase
WO1999038986A2 (en) * 1998-01-30 1999-08-05 Novartis Ag Riboflavin biosynthesis genes from plants and uses thereof
EP1010760A1 (en) * 1998-11-11 2000-06-21 E.I. Du Pont De Nemours And Company Lumazine synthase and riboflavin synthase
WO2000040744A1 (en) * 1998-12-15 2000-07-13 Adelbert Bacher Method for screening for inhibitors of riboflavin biosynthesis
WO2000044727A1 (en) * 1999-01-30 2000-08-03 Adelbert Bacher Inhibitors for the biosynthesis of vitamin b2 and method for producing same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL - TREMBL 1 November 1998 (1998-11-01), ROUNSLEY, S D ET AL., XP002160740 *
JORDAN DOUGLAS B ET AL: "Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 31, 30 July 1999 (1999-07-30), pages 22114 - 22121, XP002153695, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
AU7274800A (en) 2001-04-10
WO2001018233A9 (en) 2002-09-06
WO2001018233A2 (en) 2001-03-15
EP1208221A2 (en) 2002-05-29
DE19942175A1 (en) 2001-03-08

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