WO2001016327A3 - Verfahren zur herstellung eines kanalbildenden proteins - Google Patents

Verfahren zur herstellung eines kanalbildenden proteins Download PDF

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Publication number
WO2001016327A3
WO2001016327A3 PCT/DE2000/002924 DE0002924W WO0116327A3 WO 2001016327 A3 WO2001016327 A3 WO 2001016327A3 DE 0002924 W DE0002924 W DE 0002924W WO 0116327 A3 WO0116327 A3 WO 0116327A3
Authority
WO
WIPO (PCT)
Prior art keywords
channel
forming protein
producing
protein
forming
Prior art date
Application number
PCT/DE2000/002924
Other languages
English (en)
French (fr)
Other versions
WO2001016327A2 (de
Inventor
Michael Niederweis
Stefan Bossmann
Original Assignee
Michael Niederweis
Stefan Bossmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19943520A external-priority patent/DE19943520A1/de
Application filed by Michael Niederweis, Stefan Bossmann filed Critical Michael Niederweis
Priority to EP00969208A priority Critical patent/EP1208207A2/de
Priority to JP2001520873A priority patent/JP2003508054A/ja
Priority to CA002381139A priority patent/CA2381139A1/en
Priority to AU79013/00A priority patent/AU7901300A/en
Priority to DE10082576T priority patent/DE10082576D2/de
Publication of WO2001016327A2 publication Critical patent/WO2001016327A2/de
Publication of WO2001016327A3 publication Critical patent/WO2001016327A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/35—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Mycobacteriaceae (F)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Herstellung eines in Gram-positiven Bakterien vorkommenden kanalbildenden Proteins, wobei das kanalbildende Protein gewonnen wird durch a) heterologe Überexpression oder b) Aufreinigung aus Mycobakterien, wobei die Extraktionstemperatur mehr als 50°C beträgt.
PCT/DE2000/002924 1999-08-31 2000-08-28 Verfahren zur herstellung eines kanalbildenden proteins WO2001016327A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP00969208A EP1208207A2 (de) 1999-08-31 2000-08-28 Verfahren zur herstellung eines kanalbildenden proteins
JP2001520873A JP2003508054A (ja) 1999-08-31 2000-08-28 チャンネル形成性タンパク質の製造方法
CA002381139A CA2381139A1 (en) 1999-08-31 2000-08-28 Method for the production of a channel forming protein
AU79013/00A AU7901300A (en) 1999-08-31 2000-08-28 Method for producing a channel-forming protein
DE10082576T DE10082576D2 (de) 1999-08-31 2000-08-28 Verfahren zur Herstellung eines Kanalbildenden Proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19941416 1999-08-31
DE19941416.5 1999-08-31
DE19943520.0 1999-09-11
DE19943520A DE19943520A1 (de) 1999-08-31 1999-09-11 Verfahren zur Herstellung eines kanalbildenden Proteins

Publications (2)

Publication Number Publication Date
WO2001016327A2 WO2001016327A2 (de) 2001-03-08
WO2001016327A3 true WO2001016327A3 (de) 2001-05-31

Family

ID=26054779

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/002924 WO2001016327A2 (de) 1999-08-31 2000-08-28 Verfahren zur herstellung eines kanalbildenden proteins

Country Status (6)

Country Link
EP (1) EP1208207A2 (de)
JP (1) JP2003508054A (de)
AU (1) AU7901300A (de)
CA (1) CA2381139A1 (de)
DE (1) DE10082576D2 (de)
WO (1) WO2001016327A2 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3029467B1 (de) 2008-09-22 2020-01-08 University of Washington Msp-nanoporen und entsprechende verfahren
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
AU2015208919B9 (en) 2014-01-22 2021-04-01 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
EP3978516A1 (de) 2014-04-16 2022-04-06 The UAB Research Foundation Msp-nanoporen und verwendungen davon
WO2015166276A1 (en) 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
WO2016034591A2 (en) 2014-09-01 2016-03-10 Vib Vzw Mutant pores
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016166232A1 (en) 2015-04-14 2016-10-20 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
JP7237586B2 (ja) 2015-12-08 2023-03-13 カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント 修飾ナノポア、それを含む組成物およびその使用
EP4019542A1 (de) 2016-03-02 2022-06-29 Oxford Nanopore Technologies plc Mutante poren
US11104709B2 (en) 2016-04-06 2021-08-31 Oxford Nanopore Technologies Ltd. Mutant pore
CN110914290A (zh) 2017-06-30 2020-03-24 弗拉芒区生物技术研究所 新颖蛋白孔

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0655505A1 (de) * 1993-11-16 1995-05-31 Becton, Dickinson and Company Verfahren zum Lysieren von Mycobakterien
WO1999032634A2 (en) * 1997-12-23 1999-07-01 Genesis Research & Development Corporation Limited Compositions derived from mycobacterium vaccae and methods for their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0655505A1 (de) * 1993-11-16 1995-05-31 Becton, Dickinson and Company Verfahren zum Lysieren von Mycobakterien
WO1999032634A2 (en) * 1997-12-23 1999-07-01 Genesis Research & Development Corporation Limited Compositions derived from mycobacterium vaccae and methods for their use

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE CHEMABS [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; HOFFNER, S. E. ET AL: "Improved extraction of mycobacterial ATP", XP002159558, retrieved from DIALOG Database accession no. 125:52837 HCA *
HEINZ CHRISTIAN ET AL: "Selective extraction and purification of a mycobacterial outer membrane protein.", ANALYTICAL BIOCHEMISTRY, vol. 285, no. 1, 1 October 2000 (2000-10-01), pages 113 - 120, XP000979243, ISSN: 0003-2697 *
MICROBIOLOGY AND TUMOR BIOLOGY CENTER, KAROLINSKA INSTITUTE, STOCKHOLM, SWED. *
MUKHOPADHYAY SANJAY ET AL: "Characterization of a porin from Mycobacterium smegmatis.", JOURNAL OF BACTERIOLOGY, vol. 179, no. 19, 1997, pages 6205 - 6207, XP000979544, ISSN: 0021-9193 *
NIEDERWEIS MICHAEL ET AL: "Cloning of the mspA gene encoding a porin from Mycobacterium smegmatis.", MOLECULAR MICROBIOLOGY, vol. 33, no. 5, September 1999 (1999-09-01), pages 933 - 945, XP000979299, ISSN: 0950-382X *
SENARATNE RYAN H ET AL: "Expression of a gene for a porin-like protein of the OmpA family from Mycobacterium tuberculosis H37Rv.", JOURNAL OF BACTERIOLOGY, vol. 180, no. 14, July 1998 (1998-07-01), pages 3541 - 3547, XP000979245, ISSN: 0021-9193 *
TRIAS JOAQUIM ET AL: "Porins in the cell wall of mycobacteria.", SCIENCE (WASHINGTON D C), vol. 258, no. 5087, 1992, pages 1479 - 1481, XP002159352, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
CA2381139A1 (en) 2001-03-08
EP1208207A2 (de) 2002-05-29
AU7901300A (en) 2001-03-26
DE10082576D2 (de) 2002-12-19
WO2001016327A2 (de) 2001-03-08
JP2003508054A (ja) 2003-03-04

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