WO2000078275A2 - Antibacterial compositions - Google Patents
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- WO2000078275A2 WO2000078275A2 PCT/US2000/015729 US0015729W WO0078275A2 WO 2000078275 A2 WO2000078275 A2 WO 2000078275A2 US 0015729 W US0015729 W US 0015729W WO 0078275 A2 WO0078275 A2 WO 0078275A2
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0208—Tissues; Wipes; Patches
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/347—Phenols
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/46—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur
- A61K8/466—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing sulfur containing sulfonic acid derivatives; Salts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/02—Local antiseptics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/02—Anionic compounds
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/38—Cationic compounds
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/38—Cationic compounds
- C11D1/65—Mixtures of anionic with cationic compounds
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/83—Mixtures of non-ionic with anionic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/835—Mixtures of non-ionic with cationic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/86—Mixtures of anionic, cationic, and non-ionic compounds
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/43—Solvents
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
Definitions
- the present invention is directed to antibacterial compositions, like personal care compositions, including hand sanitizer gels, having improved antibacterial effectiveness. More particularly, the present invention is directed to antibac- terial compositions comprising an antibacterial agent and a surfactant or a relatively low amount of a disinfecting alcohol, and that provide a substantial reduction, e.g., greater than 99%, in Gram positive and Gram negative bacterial populations within one minute.
- Antibacterial personal care compositions are known in the art. Especially useful are antibacterial cleansing compositions, which typically are used to cleanse the skin and to destroy bacteria and other microorganisms present on the skin, especially the hands, arms, and face of the user.
- Another class of antibacterial personal care compositions is the hand sanitizer gels. This class of compositions is used primarily by medical personnel to disinfect the hands and fingers. The hand sanitizer gel is applied to, and rubbed into, the hands and fingers, and the composition is allowed to evaporate from the skin. Wiping of the composition from the skin is not necessary because the high alcohol content of present-day hand sani- tizer gels leads to a fast and essentially complete evaporation of the composition from the skin.
- Antibacterial compositions in general are used, for example, in the health care industry, food service industry, meat processing industry, and in the private sector by individual consumers.
- the widespread use of antibacterial compositions indicates the importance consumers place on controlling bacteria and other microorganism populations on skin. It is important, however, that antibacterial compositions provide a substantial and broad spectrum reduction in microorganism populations quickly and without problems associated with toxicity and skin irritation.
- antibacterial cleansing compositions typically contain an active antibacterial agent, a surfactant, and various other ingredients, for example, dyes, fragrances, pH adjusters, thickeners, skin conditioners, and the like, in an aqueous carrier.
- Several different classes of antibacterial agents have been used in antibacterial cleansing compositions.
- antibacterial agents examples include a bisguanidine (e.g., chlorhexidine digluconate) , diphenyl compounds, benzyl alcohols, trihalocarbanilides, quaternary ammonium compounds, ethoxylated phenols, and phenolic compounds, such as halo-substituted phenolic compounds, like PCMX (i.e., p-chloro-m-xylenol) and triclosan (i.e., 2 , 4 , 4 ' -trichloro-2 ' hydroxy-diphenylether) .
- PCMX i.e., p-chloro-m-xylenol
- triclosan i.e., 2 , 4 , 4 ' -trichloro-2 ' hydroxy-diphenylether
- Hand sanitizer gels contain a high percentage of an alcohol, like ethanol. At the high percent of alcohol present in the gel, the alcohol itself acts as a disinfectant. In addition, the alcohol quickly evaporates to obviate wiping or rinsing skin treated with the sanitizer gel.
- Hand sanitizer gels containing a high percentage of an alcohol, i.e., about 40% or greater by weight of the composition have a tendency to dry and irritate the skin.
- Antibacterial compositions generally offer a low to moderate antibacterial activity. Antibacterial activity is assessed against a broad spectrum of microorganisms, including both Gram positive and Gram negative microorganisms.
- the log reduction, or alternatively the percent reduction, in bacterial populations provided by the antibacterial composition correlates to antibacterial activity.
- a log reduction of 3-5 is most preferred, a 1-3 reduction is preferred, whereas a log reduction of less than 1 is least preferred, for a particular contact time, generally ranging from 15 seconds to 5 minutes.
- a highly preferred antibacterial composition exhibits a 3-5 log reduction against a broad spectrum of microorganisms in a short contact time.
- Prior disclosures illustrate attempts to provide such anti- bacterial compositions, which, to date, do not provide the rapid, broad range control of microorganisms desired by consumers. It should be noted that high log reductions have been achieved at pH values of 4 and 9, but such log reductions are attributed at least in part to these relatively extreme pH values. Compo- sitions having such pH values can irritate the skin and other surfaces, and, therefore, typically are avoided. This is especially the case for hand sanitizer compositions which typically are not wiped or rinsed from the skin after use. It has been difficult to impossible to achieve a high log reduction using an antibacterial composition having a neutral pH of about 5 to about 8, and especially about 6 to about 8, without simultaneously incorporating a high percentage of an alcohol.
- WO 98/01110 discloses compositions comprising triclosan, surfactants, solvents, chelating agents, thickeners, buffering agents, and water.
- WO 98/01110 is directed to reducing skin irritation by employing a reduced amount of surfactant.
- U.S. 5,635,462 discloses compositions comprising PCMX and selected surfactants.
- the compositions disclosed therein are devoid of anionic surfactants and nonionic surfac- tants.
- WO 97/46218 and WO 96/06152 disclose compositions based on triclosan, organic acids or salts, hydrotropes, and hydric solvents.
- EP 0 505 935 discloses compositions con- taining PCMX in combination with nonionic and anionic surfactants, particularly nonionic block copolymer surfactants.
- WO 95/32705 discloses a mild surfactant combination that can be combined with antibacterial compounds, like triclosan.
- WO 95/09605 discloses antibacterial compo- sitions containing anionic surfactants and alkylpolyglycoside surfactants.
- WO 98/55096 discloses antimicrobial wipes having a porous sheet impregnated with an antibacterial composition containing an active antimicrobial agent, an anionic surfactant, an acid, and water, wherein the composition has a pH of about 3.0 to about 6.0.
- A.G. Mitchell, J “ . Pharm . Pharmacol . , Vol . 16, pp. 533-537, (1964) discloses compositions con- taining PCMX and a nonionic surfactant that exhibit antibacterial activity.
- the compositions disclosed in the Mitchell publication exhibit antibacterial activity in at least 47 minutes contact time, thus the compositions are not highly effective.
- hand sanitizer gels With respect to hand sanitizer gels,
- U.S. Patent No. 5,776,430 discloses a topical antimicrobial cleaner containing chlorhexi- dine and an alcohol.
- the compositions contain about 50% to 60%, by weight, denatured alcohol and about 0.65 to 0.85%, by weight, chlorhexidine .
- the composition is applied to the skin, scrubbed into the skin, then rinsed from the skin.
- European Patent Application 0 604 848 discloses a gel-type hand disinfectant containing an antimicrobial agent, 40% to 90% by weight of an alcohol, and a polymer and a thickening agent in a combined weight of not more than 3% by weight. The gel is rubbed into the hands and allowed to evaporate to provide disinfected hands.
- the amount and identity of the antibacterial agent is not considered important because the hand sanitizer gels contain a high percentage of an alcohol to provide antibacterial activity.
- the disclosed compositions often do not provide immediate sanitization and do not provide residual antibacterial efficacy.
- Prior disclosures have not addressed the issue of which composition ingredient in an antibacterial composition provides bacterial control.
- Prior compositions also have not provided an effective, fast, and broad spectrum control of bacteria at a neutral pH of about 5 to about 8, and especially at about 6 to about 8.
- An efficacious antibacterial composition has been difficult to achieve because of the properties of the antibacterial agents and the effects of a surfactant on an antibacterial agent.
- a surfactant for example, several active antibacterial agents, like phenols, have an exceedingly low solubility in water, e.g., triclosan solubility in water is about 5 to 10 ppm (parts per million) .
- the solubility of the antibacterial agent is increased by adding surfactants to the composition.
- an increase in solubility of the antimicrobial agent, and in turn, the amount of antibacterial agent in the composi- tion does not necessarily lead to an increased antibacterial efficacy.
- a surfactant increases antimicrobial agent solubility, but also typically reduces the availability of the antibacterial agent because a surfactant in water forms micelles above the critical micelle concentration of the surfactant.
- the critical micelle con- centration varies from surfactant to surfactant.
- micelles have a lipophilic region that attracts and solubilizes the antibacterial agent, and thereby renders the antibacterial agent unavailable to imme- diately contact bacteria, and thereby control bacteria in short time period (i.e., one minute or less) .
- the antibacterial agent solubilized in the surfactant micelles will control bacteria, but in relatively long time frames.
- the antibacterial agent if free in the aqueous solution and not tied up in the surfactant micelle (i.e., is activated), is attracted to the lipophilic membrane of the bacteria and performs its function quickly. If the antibacterial agent is tied up in the surfactant micelle (i.e., is not activated), the antibacterial agent is only slowly available and cannot perform its function in a time frame that is practical for cleaning the skin.
- antibacterial agent that is solubilized in the micelle is readily washed from the skin during the rinsing process, and is not available to deposit on the skin to provide a resid- ual antibacterial benefit. Rather, the antibacterial agent is washed away and wasted.
- hand sanitizer gels typically contain: (a) at least 60% by weight ethanol or a combination of lower alcohols, such as ethanol and isopropanol, (b) water, (c) a gelling polymer, such as a crosslinked polyacrylate material, and (d) other ingredients, such as skin conditioners, fragrances, and the like.
- Hand sanitizer gels are used by consumers to effectively sanitize the hands, without, or after, washing with soap and water, by rubbing the hand sanitizer gel on the surface of the hands.
- Current commercial hand sanitizer gels rely on high levels of alcohol for disinfection and evaporation, and thus suffer from disadvantages.
- compositions containing less than 60% alcohol an additional antibacterial compound must be present to provide antibacterial activity.
- Prior disclosures have not addressed the issue of which com- position ingredient in such an antibacterial composition provides bacterial control. Therefore, for formulations containing a reduced alcohol concentration, the selection of an antibacterial agent that provides both a rapid antibacterial effect and a persistent antibacterial benefit is difficult.
- Prior compositions also have not provided an effective, fast, and broad spectrum control of bacteria at a neutral pH of about 5 to about 8, and especially at about 6 to about 8.
- an antibacterial composition that is highly efficacious against a broad spectrum of Gram positive and Gram negative bacteria in a short time period, and wherein the antibacterial activity is attributed primarily, or solely, to the presence of the active antibacterial agent in the composition.
- the present invention is directed to such antibacterial composi- tions.
- the present invention relates to antibac- terial compositions that provide a substantial reduction in Gram positive and Gram negative bacteria in less than about one minute. More particularly, in one embodiment, the present invention relates to antimicrobial compositions containing an active antibacterial agent, a surfactant, and water, wherein the antibacterial agent is present in the continuous aqueous phase (in contrast to being present in micelles) , in an amount of at least 50% of saturation, when measured at room temperature. The present invention also relates to antimicrobial compositions containing an active antibacterial agent, a surfactant, water, and a hydric solvent and/or a hydrotrope, wherein the antibacterial agent is present in an amount of at least 25% of saturation, when measured at room temperature.
- the present invention relates to antimicrobial compositions contain- ing an active antibacterial agent, a disinfecting alcohol, a gelling agent, and water, wherein the antibacterial agent is present in an amount of at least 50% of saturation, when measured at room temperature.
- the present invention also relates to antimicrobial compositions containing an active antibacterial agent, a disinfecting alcohol, a gelling agent, a hydrotrope, and water, wherein the antibacterial agent is present in an amount of at least 25% of saturation, when measured at room tem- perature.
- one aspect of the present invention is to provide a liquid, antibacterial composition
- a liquid, antibacterial composition comprising: (a) about 0.001% to about 10%, by weight, of an antibacterial agent; (b) about 0.1% to about 40%, by weight, of a surfactant selected from the group consisting of a C 8 -C 18 alkyl sulfate, a C 8 -C 18 fatty acid salt, a C 8 -C 18 alkyl ether sulfate having one or two moles of ethoxylation, a C 8 -C 18 alkamine oxide, a C 8 -C 18 alkyl sarcosinate, a C 8 -C 18 sulfoacetate, a C a -C 18 sulfosuccinate, a C 8 -C 18 alkyl diphenyl oxide disulfonate, a C 8 -C 18 alkyl carbonate, a C 8 -C 18 alpha-olefin sul
- a surfactant selected from the group consisting of an anionic surfactant, a cationic surfactant, a nonionic surfactant, an ampholytic surfactant, and mixtures thereof;
- composition contains at least one of the hydrotrope and hydric solvent, and wherein the antimicrobial agent is present in the composition in an amount of at least 25% of saturation concentration, when measured at room temperature .
- composition comprises:
- a surfactant selected from the group consisting of an anionic surfactant, a cationic surfactant, a nonionic surfactant, an ampholytic surfactant, and mixtures thereof;
- a hydrotrope 0 to about 10%, by weight, of a surfactant selected from the group consisting of an anionic surfactant, a cationic surfactant, a nonionic surfactant, an ampholytic surfactant, and mixtures thereof;
- composition contains at least one of the hydrotrope and hydric solvent in an amount sufficient to solubilize the antimicrobial agent, and wherein the antimicrobial agent is pres- ent in the composition in an amount of at least 25% of the saturation concentration, when measured at room temperature .
- Another aspect of the present invention is to provide a liquid, antibacterial composition com- prising: (a) about 0.05% to about 5%, by weight, of an antibacterial agent; (b) about 1% to about 40%, by weight, of a disinfecting alcohol, like a alcohol; (c) about 0.01% to about 5% by weight of a gelling agent, like a colloidal or a polymeric gel- ling agent; and (d) water, wherein the antibacterial agent is present in the composition in an amount of at least 50% of saturation concentration, when measured at room temperature.
- Still another aspect of the present inven- tion is to provide an alternative embodiment of the antibacterial composition, wherein the composition comprises :
- composition comprises:
- composition contains the disinfecting alcohol and an optional polyhydric solvent in an amount sufficient to solubilize the antimi- crobial agent, and wherein the antimicrobial agent is present in the composition in an amount of at least 25% of the saturation concentration, when measured at room temperature .
- Yet another aspect of the present inven- tion is to provide an antibacterial composition that exhibits a log reduction against Gram positive bacteria (i.e., S . aureus) of at least 2 after 30 seconds of contact .
- Gram positive bacteria i.e., S . aureus
- Still another aspect of the present inven- tion is to provide an antibacterial composition that exhibits a log reduction against Gram negative bacteria (i.e., E. coli ) of at least 2.5 after 30 seconds of contact .
- Another aspect of the present invention is to provide an antibacterial composition that exhibits a substantial log reduction against Gram positive and Gram negative bacteria, and has a pH of about 5 to about 8.
- Another aspect of the present invention is to provide consumer products based on an antibacterial composition of the present invention, for example, a skin cleanser, a body splash, a surgical scrub, a wound care agent, a hand sanitizer gel, a disinfectant, a mouth wash, a pet shampoo, a hard surface sanitizer, and the like.
- an antibacterial composition of the present invention for example, a skin cleanser, a body splash, a surgical scrub, a wound care agent, a hand sanitizer gel, a disinfectant, a mouth wash, a pet shampoo, a hard surface sanitizer, and the like.
- a further aspect of the present invention is to provide a method of reducing the Gram positive and/or Gram negative bacteria populations on animal tissue, including human tissue, by contacting the tissue, like the dermis, with a composition of the present invention for a sufficient time, such as about 15 seconds to 5 minutes, to reduce the bacte- ria level to a desired level, and to provide a residual control of bacteria levels.
- the composition can be wiped or rinsed from the skin. In some embodiments, the composition is allowed to remain on the skin until the volatile components of the composition evaporate.
- an antibacterial composition should provide a high log reduction against a broad spectrum of organisms in as short a contact time as possible. It also would be beneficial if the antibacterial compositions provided a residual bacterial control .
- Antibacterial hand sanitizer compositions typically do not contain a surfactant and rely upon a high concentration of an alcohol to control bacteria.
- the alcohols evaporate and, therefore, cannot provide residual bacterial control.
- the alcohols also can dry and irritate the skin.
- present invention is directed to antibacterial compositions having an exceptionally high broad spectrum antibacterial efficacy, as measured by a rapid kill of bacteria (i.e., time kill) , which is to be distinguished from per- sistent kill.
- the present antibacterial compositions provide significantly improved time kill efficacy compared to prior compositions, for example, prior sanitizer compositions that incorporate a high per- centage of an alcohol, i.e., 40% or greater, by weight.
- the basis of this improved time kill is the discovery that the antimicrobial efficacy of an active agent can be correlated to the rate at which the agent has access to an active site on the mi- crobe .
- the driving force that determines the rate of agent transport to the site of action is the difference in chemical potential between the site at which the agent acts and the external aqueous phase.
- the microbicidal activity of an active agent is proportional to its thermodynamic activity in the external phase. Accordingly, thermodynamic activity, as opposed to concentration, is the more important variable with respect to antimicrobial efficacy.
- thermodynamic activity is conveniently correlated to the percent saturation of the active antibacterial agent in the continuous aqueous phase of the composition.
- the concentration of a compound in aqueous solution can be increased over the saturation concentration in water by the addition of com- pounds like surfactants, solvents, and hydrotropes .
- Surfactants not only increase the solubility of compounds in the continuous aqueous phase of the composition, but also form micelles, and can solubilize compounds in the micelles.
- the % saturation of an active antibacterial agent in any composition, including a surfactant-containing composition ideally can be expressed as :
- % saturation [C/C xlOO% wherein C is the concentration of antibacterial agent in the composition and C s is the saturation concentration of the antibacterial agent in the composition at room temperature.
- C the concentration of antibacterial agent in the composition
- C s the saturation concentration of the antibacterial agent in the composition at room temperature.
- thermodynamic activities of the active antibacterial agent between the composition and the target organism is maximized (i.e., when the composition is more "saturated” with the active ingredient) .
- a second factor affecting anti- bacterial activity is the total amount of available antibacterial agent present in the composition, which can be thought of as the "critical dose.” It has been found that the total amount of active agent in the continuous aqueous phase of a composition greatly influences the time in which a desired level of antibacterial efficacy is achieved, given equal thermodynamic activities.
- the two key factors affecting the antibacterial efficacy of an active agent in a composition are: (1) its availability, as dictated by its thermodynamic activity, i.e., percent saturation in the continuous aqueous phase of a composition, and (2) the total amount of available active agent in the solution.
- An important ingredient in antibacterial cleansing compositions is a surfactant, which acts as a solubilizer, cleanser, and foaming agent. Surfactants affect the percent saturation of an antibacterial agent in solution, or more importantly, affect the percent saturation of the active agent in the continuous aqueous phase of the composition.
- the ratio of surfactant to antibacterial agent directly determines the amount of active agent present in the surfactant micelles, which in turn affects the percent saturation of the active agent in the continuous aqueous phase. It has been found that as the surfactant : active agent ratio increases, the number of micelles relative to active molecules also increases, with the micelles being proportionately less saturated with active agent as the ratio increases. Since the active agent in the continuous phase is in equilibrium with active agent in the micellar pseudophase, as the saturation of antibacterial agent in the micellar phase decreases, so does the saturation of the antibacterial agent in the continuous phase. The converse is also true.
- Active agent solubilized in the micellar pseudophase is not immediately available to contact the microoganisms, and it is the percent saturation of active agent in the continuous aqueous phase that determines the antibacterial activity of the composition.
- the active agent present in the surfactant micelles can serve as a reservoir of active agent to replenish the continuous aqueous phase as the active agent is depleted.
- the thermodynamic activity, or percent saturation, of an antibacterial agent in the continuous aqueous phase of a composition drives antibacterial activity. Further, the total amount of available active agent determines the ultimate extent of efficacy.
- the active agent present in surfactant micelles is not directly available for antibacterial activity.
- the percent saturation of the active agent in the composition or alternatively the percent saturation of the active agent in the continuous aqueous phase of the composition, determines antibacterial efficacy.
- an antibacterial composition of the present invention comprises: (a) about 0.001% to about 10%, by weight, of an antibacterial agent; (b) about 0.1% to about 40%, by weight, of a surfactant; (c) an optional hydric solvent; (d) an optional hydrotrope; and (e) water.
- an antibacterial composition of the present invention comprises: (a) about 0.05% to about 5%, by weight, of an antibacterial agent; (b) about 1% to about 40%, by weight, of a disinfecting alcohol; (c) about 0.01% to about 5%, by weight, of a gelling agent; (d) an optional hydrotrope; and (e) water.
- the present compositions also can contain an optional polyhydric solvent.
- the compositions can further include a hydrotrope and additional optional ingredients disclosed hereafter, like polyhydric solvents, pH adjusters, dyes, skin conditioners, vitamins, and perfumes.
- the present compositions are free of surfactants, i.e., contain 0% to about 0.5%, by weight, of compounds that exhibit surface activity.
- the compositions also are mild, and provide a persistent kill because it is not necessary to rinse or wipe the compositions from the skin.
- compositions of these embodiments, and all other embodiments have a percent saturation of antibacterial agent in the continuous aqueous phase of at least about 25%, when measured at room temperature.
- the compositions exhibit a log reduction against Gram positive bacteria of about 2 after 30 seconds contact.
- the compositions exhibit a log reduction against Gram negative bacteria of about 2.5 after 30 seconds contact.
- Antibacterial Compositions Containing an Antibacterial Agent and a Surfactant Containing an Antibacterial Agent and a Surfactant
- the antibacterial compositions comprise an active antibacterial agent, a surfactant, and water.
- the compositions of embodiment A exhibit a rapid bacteria kill even in the absence of a hydric sol- vent and a hydrotrope.
- the presence of a hydric solvent and/or a hydrotrope does not adversely affect the antimicrobial properties of the composition, but such optional ingredients are not necessary ingredients.
- the compositions can further include additional optional ingredients disclosed hereafter, like pH adjusters, dyes, and perfumes.
- An antibacterial agent is present in a composition of the present invention in an amount of about 0.001% to about 10%, and preferably about 0.01% to about 5%, by weight of the composition. To achieve the full advantage of the present invention, the antibacterial agent is present in an amount of about 0.05% to about 2%, by weight, of the composition.
- the antibacterial compositions can be ready to use compositions, which typically contain 0.001% to about 2%, preferably 0.01% to about 1.5%, and most preferably about 0.05% to about 1%, of an antibacterial agent, by weight of the composition.
- the antibacterial compositions also can be formulated as concentrates that are diluted before use with one to about 100 parts water to provide an end use composition.
- the concentrated compositions typically contain greater than about 0.1% and up to about 10%, by weight, of the antibacterial agent. Applications also are envisioned wherein the end use composition contains greater than 2%, by weight, of the antibacterial agent.
- the absolute amount of antibacterial agent present in the composition is not as important as the amount of available antibacterial agent in the composition.
- the amount of available antibacterial agent in the composition is related to the identity of the surfactant in the composition, the amount of surfactant in the compo- sition, and the presence of optional ingredients in the composition.
- the continuous aqueous phase of the composition contains an amount of antibacterial agent that is at least about 50%, and preferably at least about 75%, of the saturation concentration of the antibacterial agent in water, when measured at room temperature.
- the continuous aqueous phase is about 95% to 100% saturated with the antibacterial agent.
- the amount of antibacterial agent present in the continuous aqueous phase can be defined as the total amount of antibacterial agent in the composition, less any antibacterial agent present in surfactant micelles. The method of determining percent saturation of antibacterial agent in the composition is disclosed hereafter.
- the antimicrobial agents useful in the present invention are phenolic compounds exemplified by the following classes of compounds:
- Y is chlorine or bromine
- Z is S0 2 H, N0 2 , or alkyl
- r is 0 to 3
- o is 0 to 3
- p is 0 or 1
- m is 0 or 1
- n is 0 or 1.
- Y is chlorine or bromine
- m is 0, n is 0 or 1, o is 1 or 2, r is 1 or 2, and p is 0.
- Y is chlorine, m is 0, n is 0, o is 1, r is 2, and p is 0.
- a particularly useful 2-hydroxydiphenyl compound has the structure:
- 2-hydroxydiphenyl compound having the adopted name, triclosan, and available commercially under the tradename IRGASAN DP300, from Ciba Specialty Chemicals Corp., Greensboro, NC .
- Another useful 2-hydroxydiphenyl compound is 2,2' dihydroxy-5, 5 ' -dibromo-diphenyl ether.
- R ⁇ is hydro, hydroxy, C 1 -C 4 alkyl, chloro, nitro, phenyl, or benzyl
- R 2 is hydro, hydroxy, alkyl, or halo
- R 3 is hydro, alkyl, hydroxy, chloro, nitro, or a sulfur in the form of an alkali metal salt or ammonium salt
- R 4 is hydro or methyl
- R 5 is hydro or nitro.
- Halo is bromo or, prefera- bly, chloro.
- phenol derivatives include, but are not limited to, chlorophenols (o-, m- , p-), 2 , 4-dichlorophenol , p-nitrophenol , picric acid, xylenol, p-chloro-m-xylenol , cresols (o-, m- , P ⁇ )/ p-chloro-m-cresol, pyrocatechol, resorcinol, 4- n-hexylresorcinol, pyrogallol, phloroglucin, carvacrol, thymol, p-chlorothymol , o-phenylphenol , o-benzylphenol , p-chloro-o-benzylphenol , phenol, 4- ethylphenol, and 4-phenolsulfonic acid.
- Other phe- nol derivatives are listed in WO 98/55096, incorporated herein by reference.
- R_ and R'- are hydroxy, and R 2 , R' 2 , R 3 , R' 3 , R 4 , R' 4 , R 5 , and R' 5 , independent of one another, are hydro or halo.
- diphenyl compounds are hexachlorophene, tetrachlorophene, dichloro- phene, 2 , 3-dihydroxy-5, 5 ' -dichlorodiphenyl sulfide, 2,2' -dihydroxy-3 , 3 ' , 5, 5 ' -tetrachlorodiphenyl sulfide, 2,2' -dihydroxy-3 ,5', 5, 5', 6, 6' -hexachlorodi- phenyl sulfide, and 3 , 3 ' -dibromo-5 , 5 ' -dichloro-2 , 2 ' - dihydroxydiphenylamine .
- Other diphenyl compounds are listed in WO 98/55096, incorporated herein by reference .
- a present antimicrobial composition also contains a surfactant.
- the surfactant is present in an amount of about 0.1% to about 40%, and preferably about 0.3% to about 20%, by weight, of the composition.
- the antibacterial composition contains about 0.5% to about 15%, by weight, of the surfactant.
- Ready-to-use compositions typically contain about 0.1% to about 10%, preferably about 0.3% to about 5%, and most preferably, 0.5% to about 3%, by weight, of the composition.
- Concentrated compo- sitions suitable for dilution typically contain greater than about 5%, by weight, of a surfactant.
- the amount of surfactant present in the composition is related to the amount and identity of the antibacterial agent in the composition and to the identity of the surfactant .
- the amount of surfactant is determined such that the percent saturation of the antibacterial agent in the continuous aqueous phase of the composition is at least about 50%, preferably at least about 75%, and most prefer- ably at least about 95%.
- surfactants useful in this embodiment of the invention include anionic surfactants and selected cationic surfactants.
- Nonionic surfactants and anionic surfactants containing a relatively high amount of ethoxylation are not useful in this embodiment .
- Ethoxylated surfactants containing more than two moles of ethylene oxide have a strong affinity for the antibacterial agent, and in this embodiment substantially reduce the efficacy of the antibacterial agent.
- the surfactant is selected from the following classes of surfactants: a C 8 -C 18 alkyl sulfate, a C 8 -C 18 fatty acid salt, a C 8 -C 18 alkyl ether sulfate having one or two moles of ethoxylation, a C 8 -C ⁇ a alkamine oxide, a C 8 -C 18 alkoyl sarcosinate, a C 8 -C 18 sulfoacetate, a C 8 - C 18 sulfosuccinate, a C 8 -C 18 alkyl diphenyl oxide disulfonate, a C 8 -C 18 alkyl carbonate, a C 8 -C 18 alpha- olefin sulfonate, a methyl ester sulfonate, and mixtures thereof.
- a C 8 -C 18 alkyl sulfate a C 8 -C 18 fatty acid salt
- the C 8 -C 18 alkyl group contains eight to sixteen carbon atoms, and can be straight chain (e.g., lauryl) or branched (e.g., 2 -ethyl - hexyl) .
- the cation of the anionic surfactant can be an alkali metal (preferably sodium or potassium) , ammonium, C x -C 4 alkylammonium (mono-, di-, tri) , or C x -C 3 alkanolammonium (mono-, di-, tri-) .
- Lithium and alkaline earth cations e.g., magnesium
- Specific surfactants that can be used in this embodiment include, but are not limited to, lauryl sulfates, octyl sulfates, 2-ethylhexyl sul- fates, lauramine oxide, decyl sulfates, tridecyl sulfates, cocoates, lauroyl sarcosinates, lauryl sulfosuccinates, linear C 10 diphenyl oxide disulfo- nates, lauryl sulfosuccinates, lauryl ether sulfates (1 and 2 moles ethylene oxide), myristyl sulfates, oleates, stearates, tallates, cocamine oxide, decylamine oxide, myristamine oxide, ricinoleates, cetyl sulfates, and similar surfactants.
- CTFA Cosmetic Ingredient Handbook J.M. Nikitakis, ed., The Cosmetic, Toiletry and Fragrance Association, Inc., Washington, D.C. (1988) (hereafter CTFA Handbook), pages 10-13, 42-46, and 87-94, incorporated herein by reference. 3 .
- Carrier
- the carrier in this embodiment comprises water.
- An antibacterial composition of the present invention also can contain optional ingredients well known to persons skilled in the art.
- the composition can contain a hydric solvent and/or a hydrotrope. These particular optional ingredients and the amount that can be present in the composition are discussed hereafter.
- the compositions also can contain other optional ingredients, such as dyes and fragrances, that are present in a sufficient amount to perform their intended function and do not adversely affect the antibacterial efficacy of the composition.
- Such optional ingredients typically are present, individually, from 0% to about 5%, by weight, of the composition, and, collectively, from 0% to about 20%, by weight, of the composition.
- Classes of optional ingredients include, but are not limited to, dyes, fragrances, pH adjusters, thickeners, viscosity modifiers, buffering agents, foam stabilizers, antioxidants, foam enhancers, chelating agents, opacifiers, and similar classes of optional ingredients known to persons skilled in the art.
- alkanolamides as foam boosters and stabilizers
- gums and polymers as thickening agents
- inor- ganic phosphates, sulfates, and carbonates as buffering agents
- EDTA and phosphates as chelating agents
- acids and bases as pH adjusters.
- Examples of preferred classes of basic pH adjusters are ammonia; mono-, di-, and tri -alkyl amines; mono-, di-, and tri-alkanolamines; alkali metal and alkaline earth metal hydroxides; and mixtures thereof.
- the identity of the basic pH adjuster is not limited, and any basic pH ad- juster known in the art can be used.
- Specific, nonlimiting examples of basic pH adjusters are ammonia; sodium, potassium, and lithium hydroxide; monoethanolamine; triethylamine; isopropanolamine; diethanolamine; and triethanolamine .
- Examples of preferred classes of acidic pH adjusters are the mineral acids and polycarboxylic acids.
- Nonlimiting examples of mineral acids are hydrochloric acid, nitric acid, phosphoric acid, and sulfuric acid.
- Nonlimiting examples of polycar- boxylic acids are citric acid, glycolic acid, and lactic acid.
- the identity of the acidic pH adjuster is not limited and any acidic pH adjuster known in the art, alone or in combination, can be used.
- An alkanolamide to provide composition thickening, foam enhancement, and foam stability can be, but are not limited to, cocamide MEA, cocamide DEA, soyamide DEA, lauramide DEA, oleamide MIPA, stearamide MEA, myristamide MEA, lauramide MEA, capramide DEA, ricinoleamide DEA, myristamide DEA, stearamide DEA, oleylamide DEA, tallowamide DEA, lauramide MIPA, tallowamide MEA, isostearamide DEA, isostearamide MEA, and mixtures thereof.
- the antibacterial compositions comprise an active antibacterial agent, a surfactant, and a hydric solvent and/or a hydrotrope.
- the compositions of embodiment B exhibit a rapid bacteria kill, and are essentially unlimited in the identity of the surfactant in the composition.
- the solvent and/or hydrotrope assists in solubilizing the antibacterial agent, and reduces the affinity of the antibacterial agent to enter surfactant micelles. Accordingly, at least 25% saturation of the antibacterial agent in the continuous aqueous phase can be achieved regardless of the identity of the surfactant.
- the continuous aqueous phase of the composition contains an amount of antibacterial agent that is at least about 25%, and preferably at least about 50, and more preferably at least about 75%, of the saturation concentration of the antibacterial agent in water, when measured at room temperature.
- the continuous aqueous phase is about 95% to 100% saturated with the antibacterial agent.
- the amount of surfactant present in this embodiment of the present invention is identical to the amount disclosed above in A.2. However, due to the presence of a hydric solvent and/or a hydro- trope, the identity of the surfactant is not limited as in A.2.
- a hydric solvent and/or hydrotrope reduces the affinity of the antibacterial agent to enter surfactant micelles. Accordingly, a sufficient amount of the antibacterial agent is present in the continuous aqueous phase to quickly and effectively kill a broad spectrum of bacteria regardless of the identity of the surfactant.
- various surfactants like ethoxylated nonionic surfactants, have such a strong affinity for the antibacterial agent that the antibacterial agent is not available for a rapid bacteria kill.
- the surfactant can be an anionic surfactant, a cationic surfactant, a nonionic surfactant, or a compatible mixture of surfactants.
- the surfactant also can be an ampholytic or amphoteric surfactant, which have anionic or cationic properties depending upon the pH of the composition.
- the antibacterial compositions can contain an anionic surfactant disclosed above in A.2., and more generally can contain any anionic surfactant having a hydrophobic moiety, such as a carbon chain including about 8 to about 30 carbon atoms, and particularly about 12 to about 20 carbon atoms, and further has a hydrophilic moiety, such as sulfate, sulfonate, carbonate, phosphate, or carboxylate.
- the hydrophobic carbon chain is etherified, such as with ethylene oxide or propylene oxide, to impart a particular physical property, such as increased water solubility or reduced surface tension to the anionic surfactant .
- suitable anionic surfactants include, but are not limited to, compounds in the classes known as alkyl sulfates, alkyl ether sul- fates, alkyl ether sulfonates, sulfate esters of an alkylphenoxy polyoxyethylene ethanol, alpha-olefin sulfonates, beta-alkoxy alkane sulfonates, alkylaryl sulfonates, alkyl monoglyceride sulfates, alkyl monoglyceride sulfonates, alkyl carbonates, alkyl ether carboxylates, fatty acids, sulfosuccinates, sarcosinates , oxtoxynol or nonoxynol phosphates, taurates, fatty taurides, fatty acid amide polyoxyethylene sulfates, isethionates, or mixtures thereof. Additional anionic surfactants are listed in McCutcheon's Em
- the antibacterial compositions also can contain nonionic surfactants.
- a nonionic surfactant has a hydrophobic base, such as a long chain alkyl group or an alkylated aryl group, and a hydrophilic chain comprising a sufficient number (i.e., 1 to about 30) of ethoxy and/or propoxy moi- eties.
- nonionic surfactants examples include ethoxylated alkylphenols, ethoxylated and propoxylated fatty alcohols, polyethylene glycol ethers of methyl glucose, polyethylene glycol ethers of sorbitol, ethylene oxide-propylene oxide block copolymers, ethoxylated esters of fatty (C 8 -C 18 ) acids, condensation products of ethylene oxide with long chain amines or amides, and mixtures thereof.
- nonionic surfactants include ethoxylated alkylphenols, ethoxylated and propoxylated fatty alcohols, polyethylene glycol ethers of methyl glucose, polyethylene glycol ethers of sorbitol, ethylene oxide-propylene oxide block copolymers, ethoxylated esters of fatty (C 8 -C 18 ) acids, condensation products of ethylene oxide with long chain amines or amides, and mixtures thereof.
- nonionic surfactants include, but are not limited to, methyl gluceth-10, PEG-20 methyl glucose distearate, PEG-20 methyl glucose sesquistearate, C 11 _ 15 pareth-20, ceteth-8, ceteth-12, dodoxynol-12, laureth-15, PEG-20 castor oil, poly- sorbate 20, steareth-20, polyoxyethylene- 10 cetyl ether, polyoxyethylene- 10 stearyl ether, polyoxy- ethylene-20 cetyl ether, polyoxyethylene- 10 oleyl ether, polyoxyethylene-20 oleyl ether, an ethoxylated nonylphenol, ethoxylated octylphenol, ethoxylated dodecylphenol , or ethoxylated fatty (C 6 -C 22 ) alcohol, including 3 to 20 ethylene oxide moieties, polyoxyethylene-20 isohexadecyl ether, polyoxy- ethylene-23 glycerol
- anionic and nonionic surfactants cationic, ampholytic, and amphoteric surfactants can be used in the antimicrobial compositions.
- Cationic surfactants include amine oxides, for example.
- Ampholytic surfactants can be broadly described as derivatives of secondary and tertiary amines having aliphatic radicals that are straight chain or branched, and wherein one of the aliphatic substituents contains from about 8 to 18 carbon atoms and at least one of the aliphatic substituents contains an anionic water-solubilizing group, e.g., carboxy, sulfonate, or sulfate.
- Examples of compounds falling within this description are sodium 3- (dodecylamino) propionate, sodium 3 - (dodecylamino) - propane-1-sulfonate, sodium 2- (dodecylamino) ethyl sulfate, sodium 2- (dimethylamino) octadecanoate, disodium 3- (N-carboxymethyl -dodecylamino) propane-1- sulfonate, disodium octadecyliminodiacetate, sodium l-carboxymethyl-2-undecylimidazole, and sodium N,N- bis (2-hydroxyethyl) -2-sulfato-3 -dodecoxypropylamine . More particularly, one class of ampholytic surfactants include sarcosinates and taurates having the general structural formula
- R 1 is C n through C 21 alkyl
- R 2 is hydrogen or C 1 -C 2 alkyl
- Y is C0 2 M or S0 3 M
- M is an alkali metal
- n is a number 1 through 3.
- ampholytic surfactants is the amide sulfosuccinates having the structural formula
- ampholytic surfactants also can be used:
- ampholytic surfactants include the phosphobetaines and the phosphitaines .
- ampholytic surfactants useful in the present invention are sodium coconut N-methyl taurate, sodium oleyl N- methyl taurate, sodium tall oil acid N-methyl taurate, sodium palmitoyl N-methyl taurate, cocodi- methylcarboxymethylbetaine, lauryldimethylcarboxy- methylbetaine , lauryldimethylcarboxyethylbetaine , cetyldimethylcarboxymethylbetaine, lauryl -bis- (2- hydroxyethyl) carboxymethylbetaine, oleyldimethyl- gammacarboxypropylbetaine, lauryl-bis- (2 -hydroxy- propyl) -carboxyethylbetaine, cocoamidodimethylpro- pylsultaine, stearylamidodimethylpropylsultaine, laurylamido-bis- (2 -hydroxyethyl) propylsultaine, disodium oleamide PEG
- the carrier in this embodiment comprises water.
- This embodiment of the present invention contains 0% to about 25%, by weight, of a hydric solvent, and 0% to about 30%, by weight, of a hydrotrope, wherein the antibacterial composition contains at least one of the hydric solvent and hydrotrope.
- Preferred embodiments contain both a hydric solvent and a hydrotrope.
- Preferred embodiments contain about 2% to about 20%, by weight, of a hydric solvent and/or about 2% to about 25%, by weight, of a hydrotrope.
- Most preferred embodiments contain about 5% to about 15%, by weight, of a hydric solvent and/or about 5% to about 20%, by weight, of a hydrotrope.
- hydrocarbon solvent is a water-soluble organic compound containing one to six, and typically one to three, hydroxyl groups.
- the term “hydric solvent” therefore encompasses water-soluble alcohols, diols, triols, and polyols.
- hydric solvents include, but are not limited to, methanol, ethanol, isopropyl alcohol, n-butanol, n-propyl alcohol, ethylene glycol, propylene glycol, glycerol, diethylene glycol, dipropylene glycol, tripropylene glycol, hexylene glycol, butylene glycol, 1,2,6- hexanetriol, sorbitol, PEG-4, and similar hydroxyl - containing compounds .
- a hydrotrope is a compound that has the ability to enhance the water solubility of other compounds.
- a hydrotrope utilized in the present invention lacks surfactant properties, and typically is a short -chain alkyl aryl sulfonate.
- hydrotropes includes, but are not limited to, sodium cumene sulfonate, ammonium cumene sulfonate, ammonium xylene sulfonate, potassium toluene sulfonate, sodium toluene sulfonate, sodium xylene sulfonate, toluene sulfonic acid, and xylene sulfonic acid.
- Other useful hydrotropes include sodium polynaphthalene sulfonate, sodium polystyrene sulfonate, sodium methyl naphthalene sulfonate, and disodium succinate.
- the antibacterial compositions comprise an active antibacterial agent, and a hydric solvent and/or a hydrotrope.
- the compositions of embodiment C exhibit a rapid bacteria kill, and also are essentially unlimited in the identity of the surfactant in the composition.
- the solvent and/or hydrotrope assists in solubil- izing the antibacterial agent. Accordingly, at least 25% saturation of the antibacterial agent in the continuous aqueous phase can be achieved even in the absence of a surfactant .
- the continuous aqueous phase of the composition contains an amount of antibacterial agent that is at least about 25%, and preferably at least about 50%, and more preferably at least about 75%, of the saturation concentration of the antibacterial agent in water, when measured at room temperature.
- the continuous aqueous phase is about 95% to 100% saturated with the antibacterial agent.
- the surfactant is an optional ingredient in this embodiment. However, if present, the amount of surfactant present in this embodiment of the present invention is 0% to about 10% by weight, preferably 0% to about 5%, by weight. To achieve the full advantage of the present invention, the surfactant is present in an amount of 0% to about 2%, by weight. Due to the presence of a hydric solvent and/or a hydrotrope, the identity of the surfactant in this embodiment is identical to the surfactants disclosed in B.2.
- the carrier in this embodiment comprises water.
- the hydric solvent and hydrotrope dis- cussed in B.5. also can be utilized in this embodiment of the invention, for the same purpose.
- the amount of hydric solvent and/or hydrotrope present in this embodiment can be greater than the amount disclosed in B.5., above, because an additional amount of solvent and/or hydrotrope may be necessary to solubilize the antibacterial agent in the absence of a surfactant .
- the compositions can contain 0% to about 60%, by weight, of a hydric solvent, and 0% to about 40%, by weight, of a hydrotrope.
- the composition contains at least one of the hydrotrope and hydric solvent .
- Preferred embodiments contain about 2% to about 20%, by weight, of a hydric solvent and/or about 2% to about 25%, by weight, of a hydrotrope. Highly preferred embodiments contain about 5% to about 15%, by weight, of a hydric solvent and/or about 5% to about 20%, by weight, of a hydrotrope. Most preferred embodiments contain both a hydric solvent and a hydrotrope.
- the antibacterial compositions comprise an active antibacterial agent, a disinfecting alcohol, and a gelling agent.
- the compositions of embodiment D exhibit a rapid bacteria kill.
- the compositions of embodiment D are excellent hand sanitizers.
- the antibacterial agent in this embodiment of the invention is discussed above in A.l.
- the antibacterial agent is present in an amount of about 0.05% to about 5%, and preferably about 0.1% to about 4%, by weight of the composition.
- the antibacterial agent is present in an amount of about 0.25% to about 2%, by weight, of the composition.
- the carrier in the present composition comprises water.
- Antibacterial compositions of the present invention contain about 1% to about 40%, by weight, of a disinfecting alcohol. Preferred embodiments contain about 2% to about 38%, by weight, of a dis- infecting alcohol. Most preferred embodiments contain about 5% to about 30%, by weight, of a disinfecting alcohol.
- Disinfecting alcohol is a water-soluble alcohol containing one to six carbon atoms. Disinfecting alcohols include, but are not limited to, methanol, ethanol, propanol, and isopropyl alcohol. 4. Gelling Agent
- the present antibacterial compositions also contain about 0.01% to about 5%, by weight, and preferably 0.10% to about 3%, by weight, of a gelling agent.
- the antibacterial compositions contain about 0.25% to about 2.5%, by weight, of a gelling agent.
- the antibacterial compositions typi- cally contain a sufficient amount of gelling agent such that the composition is a viscous liquid, gel, or semisolid that can be easily applied to, and rubbed on, the skin. Persons skilled in the art are aware of the type and amount of gelling agent to include in the composition to provide the desired composition viscosity or consistency.
- gelling agent refers to a compound capable of increasing the viscosity of a water-based composition, or capa- ble of converting a water-based composition to a gel or semisolid.
- the gelling agent therefore, can be organic in nature, for example, a natural gum or a synthetic polymer, or can be inorganic in nature.
- the present compo- sitions are free of a surfactant.
- a surfactant is not intentionally added to a present antibacterial composition, but may be present in an amount of 0% to about 0.5%, by weight, because a surfactant may be present in a commercial form of a gelling agent to help dispense the gelling agent in water.
- a surfactant also may be present as an additive or byproduct in other composition ingredients. Surfactants are omitted from the present compositions to help avoid micelle formation, which in turn solubilize the active antibacterial compound and reduce its effectiveness.
- preferred gelling agents are those that do not form micelles in particular, and do not complex or bind with the active antibacterial agents, or otherwise adversely effect the antibacterial properties of the antibacterial agent. Regardless of the identity of the gelling agent, the amount of gelling agents and other composition ingredients is selected such that the antibacterial agent is present in an amount of at least 25% of saturation, when measured at room temperature . The following are nonlimiting examples of gelling agents that can be used in the present invention.
- the following compounds act primarily by thickening or gelling the aqueous portion of the compo- sition: acacia, acrylates/steareth-20 methacrylate copolymer, agar, algin, alginic acid, ammonium acrylate copolymers, ammonium alginate, ammonium chloride, ammonium sulfate, amylopectin, attapul- gite, bentonite, C9-15 alcohols, calcium acetate, calcium alginate, calcium carrageenan, calcium chloride, caprylic alcohol, carbomer 910, carbomer 934, carbomer 934P, carbomer 940, carbomer 941, carboxymethyl hydroxyethylcellulose, carboxymethyl hydroxy- propyl guar, carrageenan, cellulose, cellulose gum, cetearyl alcohol, cetyl alcohol, corn starch, damar, dextrin, dibenzylidine
- gelling agents act primarily by thickening the nonaqueous portion of the composition: abietyl alcohol, acrylinoleic acid, aluminum behenate, aluminum caprylate, aluminum dilin- oleate, aluminum distearate, aluminum isostearates/- laurates/palmitates or stearates, aluminum isostear- ates/myristates, aluminum isostearates/palmitates, aluminum isostearates/stearates , aluminum lanolate, aluminum myristates/palmitates, aluminum stearate, aluminum stearates, aluminum tristearate, beeswax, behenamide, behenyl alcohol, butadiene/acrylonitrile copolymer, C29-70 acid, calcium behenate, calcium stearate, candelilla wax, carnauba, ceresin, cholesterol, cholesteryl hydroxystearate, coconut alcohol, copal, diglyceryl stearate malate, dihydro
- a polyhydric solvent if present at all, is present in an amount of about 0.1% to about 50%, and preferably about 5% to about 50%, by weight of the composition. To achieve the full advantage of the present invention, the polyhydric solvent is present in an amount of about 10% to about 50% by weight of the composition. In contrast to a disinfecting alcohol, a polyhydric solvent contributes little, if at all, to the antibacterial efficacy of the present composition.
- polyhydric solvent is a water-soluble organic compound containing two to six, and typically two or three, hydroxyl groups.
- water-soluble means that the polyhydric solvent has a water solubility of at least 0.1 g of polyhydric solvent per 100 g of water at 25°C. There is no upper limit to the water solubility of the polyhydric solvent, e.g., the polyhydric solvent and water can be soluble in all proportions .
- polyhydric solvent therefore encompasses water-soluble diols, triols, and polyols.
- hydric solvents include, but are not limited to, ethylene glycol, propylene glycol, glycerol, diethylene glycol, di- propylene glycol, tripropylene glycol, hexylene glycol, butylene glycol, 1 , 2 , 6-hexanetriol , sorbi- tol, PEG-4, and similar polyhydroxy compounds.
- a hydrotrope if present at all, is present in an amount of about 0.1% to about 30%, and preferably about 0.5% to about 25%, by weight of the composition. To achieve the full advantage of the present invention, the hydrotrope is present in an amount of about 1% to about 20%, by weight of the composition.
- the identity of the hydrotropes is discussed in B.5., above, and is used in this em- bodiment of the invention for the same purpose.
- skin conditioners include emollients, such as, cetyl myristate, glyceryl dioleate, isopropyl myristate, lanolin, methyl laurate, PPG- 9 laurate, soy stearyl, octyl palmitate, and PPG-5 lanoate, for example.
- the skin conditioner also can be a humectant, for example, glucamine and pyridoxine glycol, for example.
- Occlusive skin conditioners for example, aluminum lanolate, corn oil, methicone, coconut oil, stearyl stearate, phenyl trimethicone, trimyristin, olive oil, and synthetic wax, also can be used.
- Combinations of the classes of skin conditioners, in addition to miscellaneous skin conditioners known to persons skilled in the art, alone or in combination can be used.
- Nonlimiting examples of miscellaneous skin conditioners include aloe, cholesterol, cystine, keratin, lecithin, egg yolk, glycine, PPG-12, retinol, salicylic acid, orotic acid, vegetable oil, and soluble animal collagen.
- the skin conditioners can be used alone, or in com- bination with a skin protectant, like petroleum, cocoa butter, calamine, and kaolin, for example.
- a skin protectant also can be used alone. Additional examples of skin conditioners and protectants can be found in "CTFA Cosmetic Ingredient Handbook, " J.M.
- Antibacterial compositions of the present invention comprising an active antibacterial agent, a disinfecting alcohol, and a hydrotrope exhibit a rapid bacteria kill.
- the alcohol and hydrotrope assist in solubilizing the antibacterial agent. Accordingly, at least 25% saturation of the antibacterial agent in the composition can be achieved even in the absence of a surfactant .
- antibacterial compositions of the present invention do not rely upon a low pH or a high pH to provide a rapid reduction in bacterial populations.
- Antibacterial compositions of the present invention can have a pH of about 4 to about 9, but at the two extremes of this pH range, the compositions can be irritating to the skin or damaging to other surfaces contacted by the composition. Accordingly, antibacterial compositions of the present invention preferably have a pH of about 5 to about 8, and more preferably about 6 to about 8. To achieve the full advantage of the present invention, the antibacterial compositions have a pH of about 6.5 to about 7.5. In addition, the antibacterial compositions of the present invention also do not rely upon a high concentration of disinfecting alcohol.
- the following Examples and Comparative Examples were prepared, and the ability of the compositions to control Gram positive and Gram negative bacteria was determined.
- the weight percentage listed in each of the following examples represents the actual, or active, weight amount of each ingredient present in the composition.
- the compositions were prepared by blending the ingredients, as understood by those skilled in the art and as described below. The following materials were used as ingredients in the examples.
- GLY Glycerin
- IPA Isopropanol
- MEA Monoethanolamine
- the following methods were used in the preparation and testing of the examples: a) Determination of Rapid Germicidal (Time Kill) Activity of Antibacterial Products.
- the activity of antibacterial compositions was measured by the time kill method, whereby the survival of challenged organisms exposed to an antibacterial test composition is determined as a function of time.
- a diluted aliquot of the compo- sition is brought into contact with a known population of test bacteria for a specified time period at a specified temperature.
- the test composition is neutralized at the end of the time period, which arrests the antibacterial activity of the composi- tion.
- the percent or, alternatively, log reduction from the original bacteria population is calculated.
- the time kill method is known to those skilled in the art.
- composition can be tested at any con- centration from 0-100%.
- concentration to use is at the discretion of the investigator, and suitable concentrations are readily determined by those skilled in the art.
- viscous samples usually are tested at 50% dilution, whereas nonviscous samples are not diluted.
- the test sample is placed in a sterile 250 ml beaker equipped with a magnetic stirring bar and the sample volume is brought to 100 ml, if needed, with sterile deionized water. All testing is performed in trip- licate, the results are combined, and the average log reduction is reported.
- the choice of contact time period also is at the discretion of the investigator. Any contact time period can be chosen. Typical contact times range from 15 seconds to 5 minutes, with 30 seconds and 1 minute being typical contact times.
- the contact temperature also can be any temperature, typically room temperature, or about 25 degrees Celsius.
- the bacterial suspension, or test inoculum is prepared by growing a bacterial culture on any appropriate solid media (e.g., agar) . The bacterial population then is washed from the agar with sterile physiological saline and the population of the bacterial suspension is adjusted to about 10 8 colony forming units per ml (cfu/ml) .
- the table below lists the test bacterial cultures used in the following tests and includes the name of the bacteria, the ATCC (American Type Culture Collection) identification number, and the abbreviation for the name of the organism used hereafter.
- S . aureus is a Gram positive bacteria
- E. coli , K. pneum, and S . choler. are Gram negative bacteria.
- the beaker containing the test composition is placed in a water bath (if constant temperature is desired) , or placed on a magnetic stirrer (if ambient laboratory temperature is desired) .
- the sample then is inoculated with 1.0 ml of the test bacteria suspension.
- the inoculum is stirred with the test composition for the predetermined contact time.
- 1.0 ml of the test composition/bacteria mixture is transferred into 9.0 ml of Tryptone-Histidine-Tween Neutralizer Solution (THT) .
- TAT Tryptone-Histidine-Tween Neutralizer Solution
- TSA+ Trypticase Soy Agar with Lecithin and Polysorbate 80
- the plates then are incubated for 25+2 hours, and the colonies are counted for the number of survivors and the calculation of percent or log reduction.
- the control count (numbers control) is determined by conducting the procedure as described above with the exception that THT is used in place of the test composition.
- the plate counts are converted to cfu/ml for the numbers control and samples, respectively, by standard microbiological methods.
- TCS solution was refiltered at room temperature before use in the study.
- a small amount of crystalline TCS was allowed to remain in the test container to ensure saturation in the event of a temperature change. It was assumed that TCS crystals present in the time kill test vessel would not affect test results because crystalline TCS is unavailable to act on the bacteria (i.e., is not solubilized) .
- TCS concentrations were calcu- lated using a linear regression line fit (Microsoft EXCEL ® software) to TCS/IPA standards included on the same HPLC run.
- aqueous TCS/surfactant compositions A French square bottle was charged with a solution containing a variable concentration of a surfactant and 0.3%, by weight, TCS.
- TCS seed crystals (about 1 mg) were added to the solution, and the mixture was allowed to stand at about 20°C. In a few days, crystals were observed on the bottom of solution containers in which the maximum solubility of TCS was exceeded.
- the approximate concentration of surfactant necessary to almost completely solubilize the 0.3% TCS was determined by use of an experimental design in which the concentration of surfactant was serially reduced by a factor of two over a series of test samples until the approximate saturation point of TCS in the surfactant was observed. Then the difference in concentration (saturated vs. just solubilized) was halved until a close endpoint for TCS saturation could be determined.
- the saturation point of TCS/surfactant compositions could be effectively estimated with small-scale (15 to 100 mL) samples, but about 600-800 g samples were required to obtain reliable final results. The initial ranges, therefore, were established with small-scale samples, and the final concentrations were determined using larger-scale samples.
- compositions containing TCS and a solvent or solvent/hydrotrope combina- tion TCS first was dissolved in the solvent used in the composition. Water then was added to the TCS/solvent composition, followed by the addition of about 1 mg of TCS seed crystals, and the resulting mixture was allowed to stand at about 20°C to crys- tallize. In compositions containing a solvent, hydrotrope, and surfactant, the TCS was dissolved in the solvent as above, and then the hydrotrope and surfactant were added to the TCS/solvent solution. The resulting mixture then was diluted to the batch total with water. Adjustment of pH also was performed, if required.
- compositions of the present invention contain a surfactant, which potentially can reduce the efficacy of the antibacterial agent.
- the following examples show the unexpected benefits achieved by compositions of the present invention.
- a composition of the present invention was compared to three commercially available antibacterial cleansing compositions in a time kill test using a contact time of 5 minutes.
- a composition of the present invention (Product A) was a saturated solution containing 0.3% triclosan in a 1.5% aqueous sodium lauryl sulfate (SLS) .
- the three commercially available antibacterial compositions having unknown triclosan concentrations were Jergens Antibacterial (JA) Hand Soap, a product of Andrew Jergens Inc.; Clean and Smooth (CS) , a prod- uct of Benckiser; and Soft Soap (SSp) , a product of Colgate Palmolive.
- JA Jergens Antibacterial
- CS Clean and Smooth
- SSp Soft Soap
- M % saturation means percent saturation of TCS in the continuous aqueous phase.
- Example 1 demonstrates the surprising improvement in log reduction of bacteria populations provided by an inventive composition compared to currently available commercial antibacterial compo- sitions.
- an aqueous composition containing triclosan in SLS, at 100% saturation offers significantly greater antibacterial efficacy than any of the three commercial products tested, against Gram positive and against Gram negative microorganisms, both of which can present a significant health threat to consumers .
- Test compositions A-l and A-2 were prepared.
- Composition A-l is a solu- tion containing 0.3% triclosan, 1.35% ammonium lauryl sulfate, with the balance being water.
- Composition A-l is 100% saturated with triclosan.
- Composition A-2 is a "placebo," i.e., an aqueous 1.35% ammonium lauryl sulfate solution that is free of the active antibacterial agent.
- composition A-l clearly provided an excellent, broad spectrum antibacterial activity, whereas the "placebo" composition A-2 exhibited an extremely limited spectrum of activity.
- Composition A-2 has especially poor efficacy against Gram negative organisms. Control of Gram negative organisms is of particular concern to consumers because such organisms present a significant health threat.
- the excellent broad spectrum activity of composition A-l clearly shows that the antibacterial activity is unambiguously attributed to the presence of the antibacterial agent in the continuous aqueous phase .
- composition A-3 contained 0.0872% by weight triclosan, 47.5% aqueous PG, and the balance being water. Composition A-3 was 100% saturated with triclosan and is a composition of the present invention.
- Test composition A-4 was a "placebo" consisting of 47.5% PG, by weight, and the balance water. This example illustrates an added advantage of including an optional hydric solvent in the composition.
- composition of the present invention provide an acceptable sani- tization efficacy even though the compositions contain a relatively low concentration of disinfecting alcohol.
- Examples B-l, B-3, and B-5 contain 0.15%, by weight, triclosan, at 100% saturation.
- Examples B-2, B-4, and B-6 are comparative examples containing 0% triclosan.
- compositions of the present invention provide a persistent antibacterial benefit because of the nonvolatile nature of the active ingredient, triclosan, whereas presently marketed compositions do not provide a persistent antibacterial activity.
- Examples B-3 through B-6 demonstrate that the rapid antibacterial activity of the present compositions is attributable mainly to the antibacterial agent, e.g., triclosan, as opposed to a disinfecting alcohol .
- the antibacterial agent e.g., triclosan
- composition B-3 contains only 28% ethanol, yet exhibits excellent broad-spectrum antibacterial activity at 15 seconds.
- Composition B-5 contains no alcohol, yet exhibits excellent antibacterial activity against S . aureus and E. coli .
- Prior art teachings rely on a high alcohol concentration (i.e., >40%) to achieve a fast, broad-spectrum antibacterial activity.
- This example illustrates the effect of the identity of the surfactant on the antibacterial activity of the composition.
- the test results summarized below were performed on a wide variety of compositions containing either an anionic surfactant or representative cationic, anionic/nonionic, amphoteric, and nonionic surfactants.
- the percent saturation of TCS in the compositions of this example is at least about 90%.
- the efficacy with respect to the cation also is unexpected (i.e., sodium, ammonium, and triethanolammonium lauryl sulfates provided high efficacy formulas, whereas lithium and magnesium lauryl sulfates did not) .
- the following table summarizes the effect of surfactant identity on the antibacterial activity of the composition. This example expands upon the data provided in Example 5.
- the table includes results of tests performed on a wide variety of compositions containing either anionic surfactants or representative examples containing cationic, anionic/nonionic, amphoteric, and nonionic surfactants .
- surfactants shows that these compositions exhibit moderate to low activity, with the exception of lauramine oxide.
- the portion of high activity of LAO is attributed to the surfactant alone because of its quasi -cationic character.
- the remaining surfactant/TCS compositions in Series V showed varied activity vs. S . aureus (Gram positive) and very little activity vs. E. coli (Gram negative) .
- This example illustrates the effect of % saturation of TCS in surfactant compositions (i.e., compositions free of a hydric solvent and hydrotrope) .
- the data summarized in the following table illustrate the effect of % saturation of TCS on the efficacy of TCS in TCS/surfactant/water compositions.
- Two sections of the table i.e., TCS/ALS compositions vs. E. coli and TCS/SOS compositions vs. S . aureus
- 100% saturated samples 0.15%TCS/0.67%ALS
- 0.15%TCS/4.0%SOS have an antibacterial activity approaching that of 100% saturated samples containing 0.3% TCS.
- the effects are seen clearly for organisms wherein the surfactant does not show a strong placebo kill effect.
- composition A-5 contains, by weight, 0.3% triclosan, 0.5% ammonium lauryl sulfate, 20% propylene glycol, and 10% sodium xylene sulfonate, with the balance water.
- Composition A-6 by weight, contains 0.1% triclosan, 0.125% ammonium xylene sulfonate, 20% propylene glycol, and 10% sodium xylene sulfonate the balance being water.
- Compositions A-5 and A-6 were 100% saturated with triclosan.
- Composition A-7 was a "placebo" containing, by weight, 0.5% ammonium lauryl sulfate, 20% propylene glycol, 10% sodium xylene sulfate, and the balance being water.
- composition A-6 (containing 0.10% triclosan) was at least as effective as composition A-5 (containing 0.3% triclosan) .
- the important feature is that both compositions were 100% saturated with triclosan.
- Example 5 also clearly showed that the active antibacterial agent is responsible for the excellent broad spectrum antibacterial activity.
- Compositions A-5 and A-6 of the invention clearly outperformed the "placebo" composition A-7, which did not contain an active antibacterial agent.
- composition B contains 1.35% ammonium lauryl sulfate (ALS) and 0.3% triclosan (TCS).
- Composition C contains 1.35% ALS and 0.0% TCS.
- Composition D contains 0.25% ALS, 14.4% DPG, 10.0% SXS, and 0.3% TCS, and Composition E contains 0.25% ALS, 14.4% DPG, 10.0% SXS with 0.0% TCS.
- Compound F contains 2.5% alkyl polyglucoside (APGTM) with 0.3% TCS.
- Compound G contains 0.3% APG, 14.4% dipropylene glycol (DPG) , 10% sodium xylene sulfonate (SXS), and 0.3% TCS.
- Compound H contains 0.3% APG with 14.4% DPG, 10% SXS, and 0.0% TCS.
- Composition I contains 1.25% sodium cocoamphoacetate (SCA) and 0.3% TCS.
- Composition J contains 0.25% SCA, 14.4% DPG, 10.0% SXS, and 0.3% TCS.
- Composition K contains 0.25% SCA, 14.4% DPG, 10.0% SXS, and 0.0% TCS.
- Composition L contains 1.75% cocamidopropyl betaine (CAPB) and 0.3% TCS.
- CAPB cocamidopropyl betaine
- Composition M contains 0.25% CAPB, 14.4% DPG, 10% SXS, and 0.3% TCS.
- Composition N contains 0.25% CAPB, 14.4% DPG, 10% SXS, and 0.0% TCS.
- Composition O contains 4% octoxynol-9 (TRITON X-100TM, TX100) .
- Composition P contains 0.75% TX100, 14.4% DPG, 10.0% SXS, and 0.3% TCS.
- Composition Q contains 1.25% sodium lauryl ether sulfate (1 EO, SLES-1) and 0.3% TCS.
- Composition R contains 0.25% SLES-1, 14.4% DPG, 10.0% SXS, and 0.3% TCS.
- compositions containing a hydric solvent and hydrotrope This example demonstrates the importance of % saturation in compositions containing a hydric solvent and hydrotrope.
- the relative % saturation of the antibacterial agent in the continuous aqueous phase of the composition also greatly influences the antibacterial activity of compositions containing a hydric solvent and hydrotrope.
- this influence on antibac- terial activity is especially apparent with respect to the Gram negative bacterium, K. pneum . o
- compositions containing an antibacterial agent, surfactant, hydric solvent, and hydrotrope are effective when a high % saturation of active antibacterial agent is maintained.
- Example 10 illustrates the effect of % saturation of TCS in compositions containing a hydric solvent, hydrotrope, and surfactant.
- the relative % saturation of the antibacterial agent in the composition also influences the antibacterial activity of a composition containing a hydric solvent and/or a hydrotrope. From the data summarized in the table of Example 10 and the following table, it is clear that a substantial gain in antibacterial efficacy (as measured by a time kill test) is associated with an increasing % saturation of the antibacterial agent in a given type of composition. The tables demonstrate this effect from two different perspectives.
- Example 10 shows the effect of changing the concentration of surfactant while main- taining the amount of other composition components constant.
- the following table shows the effect of varying the concentration of TCS while the concentration of all other components is kept constant.
- the information relating to % saturation is relative because % saturation is difficult to directly calculate. Even using this qualitative data, the effect of % saturation of TCS is clear from both tables for all organisms tested.
- compositions S, T, and U the antibacterial activity against S . aureus and K. pneum . increases, especially, with a decreasing wt% of ALS surfactant (i.e., an increase in % saturation of TCS) .
- Compositions CC, HH, MM, and RR demonstrate that about 15% SXS, or more, is preferred to exhibit high activity against K. pneum. in compositions containing a hydric solvent and a hydrotrope. This observation suggests that the hydrotrope may be acting as an adjuvant for the TCS because the time required for a substantial antibacterial kill, i.e., log reduction of at least 2, is reduced.
- the data summarized in the following table support a theory that the two primary factors for improved antibacterial efficacy are the relative amounts of surfactant and hydrotrope to the amount of antibacterial agent in compositions containing a surfactant, hydric solvent, and antibacterial agent.
- a higher percentage of surfactant can reduce the % saturation, and thereby decrease the antimicrobial activity of the composition.
- a higher percentage of hydrotrope appears to provide a higher activity against certain organisms, like K. pneum . and S . choler. It is theorized that the higher percentage of hydrotrope in the composition provides a greater amount of active antibacterial compound in the aqueous (i.e., nonmicellar) phase of the composition, thereby providing a higher time kill activity.
- the solvent therefore, may be act- ing as both an additive to enhance antimicrobial activity and to provide better physical stability in these compositions.
- products JJJJ through OOOO illustrate another effect of relative saturation of antibacterial agent in the system.
- composition KKKK has one-third the amount of TCS as composition JJJJ solubilized in the same level of ALS (0.5%), and compositions LLLL contains 0% TCS.
- composition TTTT contains slightly less ALS (0.9% vs. 1.0% for IIII), the same amount of PG (10.0%), and one-half the amount of SXS (5.0% vs. 10.0% for IIII).
- compositions 15 -A through 15 -D were prepared to demonstrate the superior germ kill provided by compositions of the present invention compared to control compositions (i.e., compo- sitions free of an antibacterial agent) , even when very low amounts of disinfecting alcohol are present.
- Compositions 15A-15D were prepared using standard mixing techniques known in the art . Table 4 below lists the composition ingredients.
- Table 5 below summarizes the antibacterial efficacy of compositions 15 -A through 15 -D, as measured in a time kill test.
- Example 15 illustrates the surprisingly high efficacy of compositions of the present invention (15-B and 15-D), wherein high log reductions are observed against both Gram positive and Gram negative bacteria, even for compositions containing less than 26% ethanol.
- the results are in contrast to compositions described in prior disclosures, wherein high alcohol concentrations (i.e., greater than about 40%) are relied upon to achieve a high, broad spectrum antibacterial activity.
- Example 16 shows that compositions of the present invention provide excellent, broad spectrum antibacterial activity, even at further reduced alcohol concentrations. Accordingly, composition 16-A containing 0.15% TCS, 11.18% ethanol, 25.71% DPG, the balance being water (as weight percent of active compounds) , was prepared. For comparison, an identical control composition 16-B was prepared, except composition 16-B was free of TCS.
- TCS time kill test
- Example 16 further demonstrates that the concentration of alcohol in the present compositions can be reduced to very low levels without sacrificing antibacterial activity. Accordingly, compositions that provide excellent antibacterial efficacy, and that do not dry the skin, can be prepared. Prior compositions that relied on a high alcohol concentration for antibacterial activity dried the skin, and often caused skin irritation.
- Example 17 demonstrates that highly effective compositions of the present invention can in- corporate p-chloro-m-xylenol (PCMX) as the antibacterial active agent.
- Composition 17 -A was prepared by admixing 0.1% PCMX, 13.42% ethanol, and the balance water (as weight percent of active compounds) .
- the antibacterial efficacy of composition 17 -A was evaluated by a time kill test and exhibited log reductions against S . aureus, E. coli , K. pneum. , and S . chol . , at 30 seconds contact time, of 4.16, >4.34, 3.99, and >4.04, respectively.
- composition 17-A is a highly effective antibacterial composition, even though the composition contained a very low concentration of ethanol.
- Example 18 illustrates a composition of the present invention containing a cationic gelling agent, CELQUAT CS-230M.
- Composition 18-A was prepared by admixing 0.15% TCS, 28% ethanol, 11.18% DPG, and 2% CELQUAT CS-230M, and the balance was water (as weight percent of active compounds, except CELQUAT, which is "as-is") .
- the antibacterial efficacy of composition 18-A was evaluated by a time kill test.
- Composition 18-A demonstrated the foilowing log reductions against S . aureus, E. coli , K. pneum. , and S . chol . , at 30 seconds contact time of >3.83, 4.33, >4.43, and >3.55, respectively.
- composition 18-A is a highly effective antibacterial composition, even though the composition contained a very low concentration of ethanol.
- compositions of the present invention can contain a wide variety of gelling agents, hydric solvents, and antibacterial active agents, illustrated by the following examples.
- Table 6 all weight percentages are as active material, except where indicated by a "*,” which indicates an "as-is” weight.
- the compositions were prepared by mixing and gel preparation techniques well known to persons skilled in the art. The compositions exhibited acceptable clarity, stability, and performance. I- 1 to
- % saturation of antibacterial agent in the aqueous phase of the composition can be directly correlated to a log reduction of bacteria.
- a composition having 50% saturation of TCS in the aqueous phase demonstrates a log reduction versus S. aureus of 1.96 (30 seconds) and 3.05 (60 seconds) and a log reduction versus E. coli of 2.45 (30 seconds) and greater than 3.81 (60 seconds) .
- a 75% saturated and a 100% saturated composition exhibited a log reduction of greater than 4.55 (30 and 60 seconds) vs. S. aureus (i.e., a log reduction in excess of the detection limit of the assay) .
- the 75% and 100% satu- rated compositions exhibited a log reduction of 3.40 (30 seconds) and greater than 3.81 (60 seconds) and greater than 3.81 (30 and 60 seconds) vs. E. coli , respectively.
- the present antibacterial compositions can be characterized as exhibiting a log reduction of at least about 2 (after 30 seconds) or at least about 3 (after 60 seconds) vs. S . aureus, or of at least about 2.5 (after 30 seconds) or at least about 3.5 (after 60 seconds) vs. E. coli .
- the antibacterial compositions of the present invention have several practical end uses, including hand cleansers, mouthwashes, surgical scrubs, body splashes, hand sanitizer gels, and similar personal care products.
- compositions include foamed compositions, such as creams, mousses, and the like, and compositions containing organic and inorganic filler materials, such as emulsions, lotions, creams, pastes, and the like.
- the compositions further can be used as an antibacterial cleanser for hard surfaces, for example, sinks and countertops in hospitals, food service areas, and meat processing plants.
- the present antibacterial compositions can be manufactured as dilute ready-to-use compositions, or as concentrates that are diluted prior to use.
- compositions also can be incorporated into a web material to provide an antibacterial wiping article.
- the wiping article can be used to clean and sanitize skin or inanimate surfaces.
- the present antimicrobial compositions provide the advantages of a broad spectrum kill of Gram positive and Gram negative bacteria in short contact times.
- the short contact time for a substantial log reduction of bacteria is important in view of the typical 15 to 60 second time frame used to cleanse and sanitize the skin and inanimate surfaces .
- the present compositions are effective in short contact time because the antibacterial agent is present in the aqueous continuous phase of the composition, as opposed to surfactant micelles.
- the antibacterial agent therefore, is available to immediately begin reducing bacterial populations, and further is available to deposit on the skin to provide residual antibacterial efficacy.
- the antibacterial agent is in solution as opposed to surfactant micelles, the absolute amount of antimicrobial agent in the composition can be reduced without adversely affecting efficacy, and the antibacterial agent is not rinsed from the skin with the surfactant prior to performing its antibac- terial function.
- the amount of surfactant in the present antibacterial compositions typically is low, thereby providing additional environmental benefits.
- composition in accordance with the instant invention suitable for use as a hand wash, was prepared.
- the composition contained the following components in the indicated weight percentages:
- the composition was prepared by admixing the dipropylene glycol, TCS, and fragrance until homogeneous (about 5 minutes) . After the triclosan was completely dissolved, as evidenced by the absence of undissolved solid material, the sodium xylene sulfonate was added to the solution. The resulting mixture then was stirred to completely dissolve the sodium xylene sulfonate (about 5 minutes) . Finally, the ammonium lauryl sulfate and water were added to the resulting solution, and the composition was stirred until homogeneous (about 5 minutes) .
- the composition had a weight ratio of surfactant : triclosan of 2.5:1, and was at least about 90% saturated with triclosan.
- the composition was evaluated for antibacterial efficacy against S. aureus and E. coli using a time kill test. Against S . aureus, the composition exhibited a log reduction of >4.07 in 30 seconds, while against E. coli the composition exhibited a log reduction of 3.90 in 30 seconds. Thus, the composition exhibited an excellent broad spectrum antibacterial activity. Also, the composition was an excellent hand wash composi- tion in an actual use test, providing both good cleansing and a smooth feel to the hands .
- composition in accordance with the present invention suitable for use as a body splash, is prepared using the following ingredients in the following weight percentages:
- the composition is prepared by combining the triclosan, propylene glycol, fragrance, and ethanol, and admixing the components until all the triclosan is dissolved, as evidenced by the absence of undissolved solid material.
- the sodium xylene sulfonate then is added, and the resulting mixture is stirred until the sodium xylene sulfonate is completely dissolved.
- the alkyl polyglycoside and water are added, and the mixture again is stirred until homogeneous.
- the resulting composition forms an excellent and refreshing body splash that provides a desirable level of bacterial reduction on the skin of the user.
- composition in accordance with the pres- ent invention, suitable for use as a mouthwash is prepared using the following ingredients in the following weight percentages:
- the composition is prepared by combining the triclosan, propylene glycol, flavor, and denatured alcohol, and admixing the components by any conventional means until all the triclosan is dissolved, as evidenced by the absence of undissolved solid material. Then, the sodium xylene sulfonate is added, and the resulting mixture is stirred until the sodium xylene sulfonate is completely dissolved. Finally, the alkyl polyglycoside and water are added, and the mixture again is stirred until homogeneous. The resulting composition forms an excellent and refreshing mouthwash that provides a desirable level of bacterial reduction on the teeth, gums, and tongue of the user.
- composition in accordance with the present invention suitable for impregnating a nonwoven material for the preparation of a wet wipe article, was prepared using the following ingredients in the following weight percentages:
- the composition was prepared by combining the triclosan and dipropylene glycol, and admixing the components until all the triclosan was dissolved, as evidenced by the absence of undissolved solid material.
- the sodium xylene sulfonate then was added, and the resulting mixture was stirred until the sodium xylene sulfonate was completely dissolved.
- the ammonium lauryl sulfate and water were added, and the mixture was again stirred until homogeneous.
- a piece of nonwoven cellulosic web material i.e., a commercial paper towel
- the article formed an excellent wet wipe and the impregnated antibacterial composition was freely expressed from the web to provide a broad spectrum antibacterial activity.
- composition in accordance with the present invention suitable for use as a hand wash, was prepared.
- the composition comprised the following components at the indicated weight percentages:
- the composition was prepared by first admixing the triclosan and dipropylene glycol until homogeneous (about 5 minutes) . After the triclosan was completely dissolved, as evidenced by the absence of undissolved solid material, the sodium xylene sulfonate was added to the solution. The mixture then was stirred to completely dissolve the sodium xylene sulfonate (about 5 minutes) . Finally, the ammonium lauryl sulfate and water were added to the resulting solution, and the composition was stirred until homogeneous (about 5 minutes) .
- the composition had a weight ratio of surfactant : triclosan of 2.5:1 and was at least about 90% saturated with triclosan.
- the composition was evaluated for its antibacterial efficacy against S . aureus, E. coli , K. pneum. , and S . choler. using a time kill test, and a contact time of 30 seconds.
- the composition exhibited log reductions of >3.59, >4.49, >3.20, and >4.27 against the four test organisms, respectively.
- the composition exhibited an excellent broad spectrum antibacterial activity.
- the composition was an excellent hand wash composition in an actual use test, providing both good cleansing and a smooth feel to the hands.
- composition of Example 24 was compared to the sole example disclosed in WO 98/01110.
- the active antibacterial agent was triclosan (TCS) .
- TCS triclosan
- Both compositions were evaluated for antibacterial efficacy in a time kill test against S . aureus, E. coli , K. pneum . , and S . choler.
- the example of WO 98/01110 was tested at 50% dilution, in accordance with the test procedure for viscous compositions.
- test dilution 100% for the composition of Example 24 and 50% for the example of WO 98/01110
- log reduction observed in the time kill test at a contact time of 30 seconds summarizes the percent of active antibacterial agent in each composition at the test dilution (i.e., test dilution is 100% for the composition of Example 24 and 50% for the example of WO 98/01110)
- composition of the present invention demonstrates the superior time kill performance of a composition of the present invention compared to a prior composition, especially against Gram negative bacteria. This superiority is demonstrated even through the comparative composition contained substantially more active antibacterial agent compared to the inventive composition.
- an inventive composition utilizes the active agent more efficiently, as illustrated in a higher log reduction using a reduced concentration of antibacterial agent.
- WO 96/06152 discloses effective compositions comprising TCS, an anionic surfactant, a hydrotrope, a hydric solvent, and further comprising an organic acid, specifically citric acid.
- WO 96/06152 contains additional pH adjusting agents, such as monoethanolamine and so- dium hydroxide. Further, the examples disclosed in WO 96/06152 all have a pH of 4 or 9.1, with no examples having a desirable, neutral pH of about 7.
- composition 26-A A pH of about 7 is desired for compositions contacting skin or inanimate surfaces because compositions of pH substantially different from 7, such as 4 or 9.1, have a greater potential to damage the surfaces they contact. Accordingly, the composition of Example 1 of WO 96/06152 (hereafter referred to as composition 26-A) was prepared. For comparison, composition
- composition 26 -A was prepared as above, except that the pH was adjusted to 7 by the addition of further monoethanolamine (this composition hereafter referred to as composition 26-B) .
- composition 26-C the composition of Example 3 of WO 96/06152 was prepared, except that it was prepared at a pH of 7 by the addition of further monoethanolamine (this composition is hereafter referred to as composition 26-C) .
- the table below summarizes the results of a time kill test on the compositions of this example against the bacteria indicated at a contact time of 30 seconds.
- PCMX p-chloro-m- xylenol
- the composition was prepared by first mixing the PCMX and ethanol to completely solubilize the PCMX (about 5 minutes) . After the PCMX was completely dissolved, as evidenced by the absence of undissolved solid material, the water was added, and the composition was stirred until homogeneous (about 5 minutes) .
- the composition was at least about 90% saturated with PCMX.
- the composition was evaluated for antibacterial efficacy against S . aureus, E. col , K. pneum . , and S . choler. using a time kill test.
- S . aureus the composition exhibited a log reduction of 4.16 in 30 seconds; against E. coli the composition exhibited a log reduction of >4.34 in 30 seconds; against K. pneum. the composition exhibited a log reduction of 3.99 in 30 seconds; and against S. choler. the composition exhibited a log reduction of >4.04 in 30 seconds.
- the composition exhibited an excellent broad spectrum antibacterial activity.
- composition in accordance with the present invention incorporating p-chloro-m-xylene as the active antibacterial ingredient was prepared.
- the composition contained the following components in the indicated weight percentages :
- the composition was prepared by first combining the PCMX and water, then adding the ammonium lauryl sulfate and mixing the components for such time as to completely admix the components and dissolve the PCMX (about 2 hours) .
- the composition was at least about 90% saturated with PCMX.
- the composition was evaluated for its antibacterial efficacy against S. aureus and E. coli using a time kill test. Against S . aureus, the composition exhibited a log reduction of >3.57 in 30 seconds; and against E. coli the composition exhibited a log reduction of >4.17 in 30 seconds. Thus, the composition exhibited an excellent broad spectrum antibacterial activity.
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- Medicinal Preparation (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR0011860-5A BR0011860A (en) | 1999-06-23 | 2000-06-08 | Antibacterial compositions and process for reducing a population of bacteria on a surface |
MXPA01013312A MXPA01013312A (en) | 1999-06-23 | 2000-06-08 | Antibacterial compositions. |
EP00939667A EP1191843A2 (en) | 1999-06-23 | 2000-06-08 | Antibacterial compositions |
AU54720/00A AU777059B2 (en) | 1999-06-23 | 2000-06-08 | Antibacterial compositions |
JP2001504341A JP2003502353A (en) | 1999-06-23 | 2000-06-08 | Antimicrobial composition |
CA002371925A CA2371925C (en) | 1999-06-23 | 2000-06-08 | Antibacterial compositions |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/338,654 US6107261A (en) | 1999-06-23 | 1999-06-23 | Compositions containing a high percent saturation concentration of antibacterial agent |
US09/338,654 | 1999-06-23 | ||
US09/425,521 | 1999-10-22 | ||
US09/425,521 US6136771A (en) | 1999-06-23 | 1999-10-22 | Compositions containing a high percent saturation concentration of antibacterial agent |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2000078275A2 true WO2000078275A2 (en) | 2000-12-28 |
WO2000078275A3 WO2000078275A3 (en) | 2001-09-27 |
Family
ID=26991292
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2000/015729 WO2000078275A2 (en) | 1999-06-23 | 2000-06-08 | Antibacterial compositions |
Country Status (8)
Country | Link |
---|---|
EP (1) | EP1191843A2 (en) |
JP (1) | JP2003502353A (en) |
AU (1) | AU777059B2 (en) |
BR (1) | BR0011860A (en) |
CA (1) | CA2371925C (en) |
MX (1) | MXPA01013312A (en) |
TW (1) | TWI284020B (en) |
WO (1) | WO2000078275A2 (en) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004004677A1 (en) * | 2002-07-10 | 2004-01-15 | The Dial Corporation | Compositions having enhanced deposition of a topically active on a surface |
WO2004080179A1 (en) * | 2003-03-10 | 2004-09-23 | Xantech Pharmaceuticals, Inc. | Surface sanitizing compositions with improved antimicrobial performance |
WO2006062835A2 (en) * | 2004-12-09 | 2006-06-15 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
WO2006062897A2 (en) * | 2004-12-09 | 2006-06-15 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
WO2006062846A2 (en) * | 2004-12-09 | 2006-06-15 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
EP2705105A4 (en) * | 2012-06-13 | 2015-08-19 | Green On Ind Inc | Solvent-free oil dispersant |
WO2017063848A1 (en) * | 2015-10-12 | 2017-04-20 | Unilever N.V. | Sanitising and disinfecting compositions |
CN112898696A (en) * | 2021-01-21 | 2021-06-04 | 南亚塑胶工业(南通)有限公司 | Preparation method of mildew-proof antibacterial adhesive tape |
US11419797B2 (en) | 2017-12-27 | 2022-08-23 | Kao Corporation | Method for producing composition containing hardly water-soluble aromatic compound |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005029532A (en) * | 2003-07-09 | 2005-02-03 | Asahi Kasei Chemicals Corp | Skin-cleansing agent |
US7268165B2 (en) * | 2004-08-20 | 2007-09-11 | Steris Inc. | Enhanced activity alcohol-based antimicrobial compositions |
GB0802189D0 (en) * | 2008-02-07 | 2008-03-12 | Reckitt Benckiser Inc | Topical antimicrobial compositions |
US9451763B2 (en) | 2008-04-08 | 2016-09-27 | Laboratoire M2 | Disinfectant formulation |
US10111425B2 (en) | 2008-04-08 | 2018-10-30 | Laboratoire M2 | Disinfectant formulation |
BRPI0911317B1 (en) * | 2008-04-08 | 2019-06-04 | Laboratoire M2 | AQUOSA DISINFECTANT SOLUTION FORMULATION, ITS PREPARATION METHOD AND METHOD OF DISINFECTING A SURFACE |
US9609864B2 (en) | 2008-04-08 | 2017-04-04 | Laboratoire M2 | Disinfectant formulation |
US10405544B2 (en) | 2008-04-08 | 2019-09-10 | Laboratoire M2 | Disinfectant formulation |
EP2436754A1 (en) * | 2011-09-30 | 2012-04-04 | Basf Se | Antimicrobial cleaning compound |
JP6956462B2 (en) * | 2015-10-07 | 2021-11-02 | 花王株式会社 | Absorbent article |
JP6800585B2 (en) * | 2015-01-19 | 2020-12-16 | 花王株式会社 | Water-absorbent resin composition and its manufacturing method |
CN107206353A (en) * | 2015-01-19 | 2017-09-26 | 花王株式会社 | Absorbent commodity material, its manufacture method and use its absorbent commodity |
EP3258782A4 (en) * | 2015-02-16 | 2018-07-25 | Laboratoire M2 | Aqueous compositions for controlling pests or regulating plant growth, said compositions comprising thymol or carvacrol, a surfactant, and a solvent |
JP6785600B2 (en) * | 2015-08-31 | 2020-11-18 | アース製薬株式会社 | Sterilizing agent for hard surface and sterilizing method |
JP2019071853A (en) * | 2017-10-18 | 2019-05-16 | 北海道公立大学法人 札幌医科大学 | Methods for screening antimicrobial agents |
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GB743984A (en) * | 1952-09-03 | 1956-01-25 | Monsanto Chemicals | Improvements relating to germicides |
US3326808A (en) * | 1965-08-25 | 1967-06-20 | Pfizer & Co C | Antiseptic detergent composition |
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FR2629095A1 (en) * | 1988-03-22 | 1989-09-29 | Reckitt Colman | Cleaning composition for hard surfaces |
EP0505935A1 (en) * | 1991-03-25 | 1992-09-30 | Becton, Dickinson and Company | Ph controlled antimicrobial formulation |
US5288486A (en) * | 1985-10-28 | 1994-02-22 | Calgon Corporation | Alcohol-based antimicrobial compositions |
EP0604848A2 (en) * | 1992-12-28 | 1994-07-06 | Toko Yakuhin Kogyo Kabushiki Kaisha | Quick-drying gel-type disinfectant for hands and fingers |
US5403864A (en) * | 1993-04-01 | 1995-04-04 | John A. Manfuso, Jr. | Rapidly-acting topical antimicrobial composition |
WO1995009605A1 (en) * | 1993-10-06 | 1995-04-13 | Henkel Corporation | Improving phenolic disinfectant cleaning compositions with alkylpolyglucoside surfactants |
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EP0855440A1 (en) * | 1997-01-24 | 1998-07-29 | The Procter & Gamble Company | Antibacterial liquid dishwashing detergent compositions |
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JPS6345217A (en) * | 1986-07-23 | 1988-02-26 | チバ−ガイギ アクチエンゲゼルシヤフト | Fungicidal composition |
JPS63222766A (en) * | 1987-03-12 | 1988-09-16 | 株式会社ハッチ | Preparatory sanitation treatment of beddings |
AU3001889A (en) * | 1988-02-17 | 1989-08-17 | Ciba-Geigy Ag | Antimicrobial soap composition |
HUT76688A (en) * | 1994-08-25 | 1997-10-28 | Ciba Geigy Ag | Surface-active formulations and useing them |
EP0996419A1 (en) * | 1997-06-04 | 2000-05-03 | The Procter & Gamble Company | Liquid antimicrobial cleansing compositions which provide residual benefit versus gram negative bacteria |
-
2000
- 2000-06-08 BR BR0011860-5A patent/BR0011860A/en not_active Application Discontinuation
- 2000-06-08 CA CA002371925A patent/CA2371925C/en not_active Expired - Fee Related
- 2000-06-08 EP EP00939667A patent/EP1191843A2/en not_active Ceased
- 2000-06-08 MX MXPA01013312A patent/MXPA01013312A/en active IP Right Grant
- 2000-06-08 JP JP2001504341A patent/JP2003502353A/en active Pending
- 2000-06-08 AU AU54720/00A patent/AU777059B2/en not_active Ceased
- 2000-06-08 WO PCT/US2000/015729 patent/WO2000078275A2/en active IP Right Grant
- 2000-06-23 TW TW089112409A patent/TWI284020B/en not_active IP Right Cessation
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US3326808A (en) * | 1965-08-25 | 1967-06-20 | Pfizer & Co C | Antiseptic detergent composition |
US5288486A (en) * | 1985-10-28 | 1994-02-22 | Calgon Corporation | Alcohol-based antimicrobial compositions |
EP0259249A2 (en) * | 1986-07-23 | 1988-03-09 | Ciba-Geigy Ag | Microbicidal preparations |
FR2629095A1 (en) * | 1988-03-22 | 1989-09-29 | Reckitt Colman | Cleaning composition for hard surfaces |
EP0505935A1 (en) * | 1991-03-25 | 1992-09-30 | Becton, Dickinson and Company | Ph controlled antimicrobial formulation |
EP0604848A2 (en) * | 1992-12-28 | 1994-07-06 | Toko Yakuhin Kogyo Kabushiki Kaisha | Quick-drying gel-type disinfectant for hands and fingers |
US5403864A (en) * | 1993-04-01 | 1995-04-04 | John A. Manfuso, Jr. | Rapidly-acting topical antimicrobial composition |
WO1995009605A1 (en) * | 1993-10-06 | 1995-04-13 | Henkel Corporation | Improving phenolic disinfectant cleaning compositions with alkylpolyglucoside surfactants |
US5635462A (en) * | 1994-07-08 | 1997-06-03 | Gojo Industries, Inc. | Antimicrobial cleansing compositions |
WO1996006152A2 (en) * | 1994-08-25 | 1996-02-29 | Ciba Specialty Chemicals Holding Inc. | Surface-active formulations |
DE19530833A1 (en) * | 1994-08-25 | 1996-02-29 | Ciba Geigy Ag | Surfactant mixts. for use as disinfectants |
WO1997046218A2 (en) * | 1996-06-04 | 1997-12-11 | Ciba Specialty Chemicals Holding Inc. | Concentrated liquid formulations comprising a microbicidally active ingredient |
EP0855440A1 (en) * | 1997-01-24 | 1998-07-29 | The Procter & Gamble Company | Antibacterial liquid dishwashing detergent compositions |
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Title |
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CHEMICAL ABSTRACTS, vol. 122, no. 2, 9 January 1995 (1995-01-09) Columbus, Ohio, US; abstract no. 16825, KJAERHEIM, V. ET AL: "Biological activities of different triclosan-detergent combinations" XP002150726 & COLLOIDS SURF., B (1994), 3(1/2), 19-24 , 1994, * |
DATABASE WPI Section Ch, Week 198946 Derwent Publications Ltd., London, GB; Class D21, AN 1989-292819 XP002164623 -& JP 01 249897 A (CIBA GEIGY AG), 5 October 1989 (1989-10-05) & AU 30018 89 A (CIBA-GEIGY) 17 August 1989 (1989-08-17) * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004004677A1 (en) * | 2002-07-10 | 2004-01-15 | The Dial Corporation | Compositions having enhanced deposition of a topically active on a surface |
AU2003247720B2 (en) * | 2002-07-10 | 2009-03-26 | The Dial Corporation | Compositions having enhanced deposition of a topically active on a surface |
WO2004080179A1 (en) * | 2003-03-10 | 2004-09-23 | Xantech Pharmaceuticals, Inc. | Surface sanitizing compositions with improved antimicrobial performance |
WO2006062897A3 (en) * | 2004-12-09 | 2007-01-18 | Dial Corp | Compositions having a high antiviral and antibacterial efficacy |
WO2006062846A2 (en) * | 2004-12-09 | 2006-06-15 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
WO2006062835A3 (en) * | 2004-12-09 | 2007-01-04 | Dial Corp | Compositions having a high antiviral and antibacterial efficacy |
WO2006062897A2 (en) * | 2004-12-09 | 2006-06-15 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
WO2006062846A3 (en) * | 2004-12-09 | 2007-01-18 | Dial Corp | Compositions having a high antiviral and antibacterial efficacy |
WO2006062835A2 (en) * | 2004-12-09 | 2006-06-15 | The Dial Corporation | Compositions having a high antiviral and antibacterial efficacy |
EP2705105A4 (en) * | 2012-06-13 | 2015-08-19 | Green On Ind Inc | Solvent-free oil dispersant |
WO2017063848A1 (en) * | 2015-10-12 | 2017-04-20 | Unilever N.V. | Sanitising and disinfecting compositions |
US11419797B2 (en) | 2017-12-27 | 2022-08-23 | Kao Corporation | Method for producing composition containing hardly water-soluble aromatic compound |
CN112898696A (en) * | 2021-01-21 | 2021-06-04 | 南亚塑胶工业(南通)有限公司 | Preparation method of mildew-proof antibacterial adhesive tape |
Also Published As
Publication number | Publication date |
---|---|
AU777059B2 (en) | 2004-09-30 |
MXPA01013312A (en) | 2003-09-04 |
TWI284020B (en) | 2007-07-21 |
CA2371925A1 (en) | 2000-12-28 |
WO2000078275A3 (en) | 2001-09-27 |
BR0011860A (en) | 2002-04-30 |
JP2003502353A (en) | 2003-01-21 |
AU5472000A (en) | 2001-01-09 |
EP1191843A2 (en) | 2002-04-03 |
CA2371925C (en) | 2009-02-17 |
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