WO2000047748A3 - Udp-glucose modifiers - Google Patents

Udp-glucose modifiers Download PDF

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Publication number
WO2000047748A3
WO2000047748A3 PCT/US2000/003513 US0003513W WO0047748A3 WO 2000047748 A3 WO2000047748 A3 WO 2000047748A3 US 0003513 W US0003513 W US 0003513W WO 0047748 A3 WO0047748 A3 WO 0047748A3
Authority
WO
WIPO (PCT)
Prior art keywords
udp
glucose pyrophosphorylase
udp glucose
relates
chimeric gene
Prior art date
Application number
PCT/US2000/003513
Other languages
French (fr)
Other versions
WO2000047748A2 (en
Inventor
Omolayo O Famodu
Richard W Pearlstein
Original Assignee
Du Pont
Omolayo O Famodu
Richard W Pearlstein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Omolayo O Famodu, Richard W Pearlstein filed Critical Du Pont
Priority to CA2359811A priority Critical patent/CA2359811C/en
Priority to US09/913,060 priority patent/US6982363B1/en
Priority to EP00910141A priority patent/EP1151111A2/en
Priority to AU32283/00A priority patent/AU3228300A/en
Publication of WO2000047748A2 publication Critical patent/WO2000047748A2/en
Publication of WO2000047748A3 publication Critical patent/WO2000047748A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a UDP glucose pyrophosphorylase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the UDP glucose pyrophosphorylase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the UDP glucose pyrophosphorylase in a transformed host cell.
PCT/US2000/003513 1999-02-10 2000-02-09 Udp-glucose modifiers WO2000047748A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA2359811A CA2359811C (en) 1999-02-10 2000-02-09 Udp-glucose modifiers
US09/913,060 US6982363B1 (en) 1999-02-10 2000-02-09 UDP-glucose modifiers
EP00910141A EP1151111A2 (en) 1999-02-10 2000-02-09 Udp-glucose modifiers
AU32283/00A AU3228300A (en) 1999-02-10 2000-02-09 Udp-glucose modifiers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11958899P 1999-02-10 1999-02-10
US60/119,588 1999-02-10

Publications (2)

Publication Number Publication Date
WO2000047748A2 WO2000047748A2 (en) 2000-08-17
WO2000047748A3 true WO2000047748A3 (en) 2000-12-14

Family

ID=22385197

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2000/003453 WO2000047755A2 (en) 1999-02-10 2000-02-09 Plant udp-glucose epimerases
PCT/US2000/003513 WO2000047748A2 (en) 1999-02-10 2000-02-09 Udp-glucose modifiers

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2000/003453 WO2000047755A2 (en) 1999-02-10 2000-02-09 Plant udp-glucose epimerases

Country Status (5)

Country Link
EP (2) EP1151115A2 (en)
AR (1) AR022567A1 (en)
AU (2) AU3228300A (en)
CA (3) CA2359811C (en)
WO (2) WO2000047755A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103725658A (en) * 2013-12-23 2014-04-16 内蒙古大学 Dahurian-larch-derived plant-development-related protein LgUGPase, and coding gene and application thereof

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002070649A2 (en) * 2001-02-01 2002-09-12 Ziyu Dai Epimerase gene and use thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07327679A (en) * 1994-06-06 1995-12-19 Toyota Motor Corp Dna sequence coding udp glucose synthetase
US5646023A (en) * 1993-04-15 1997-07-08 J.R. Simplot Company Modulation of sugar content in plants

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9710990D0 (en) * 1997-05-28 1997-07-23 Danisco Modification process

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646023A (en) * 1993-04-15 1997-07-08 J.R. Simplot Company Modulation of sugar content in plants
JPH07327679A (en) * 1994-06-06 1995-12-19 Toyota Motor Corp Dna sequence coding udp glucose synthetase

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DATABASE EMEST12 EMBL Heidelberg, Germany; 18 May 1999 (1999-05-18), WALBOT V: "Maize ESTs from various cDNA libraries sequenced at Stanford University", XP002141584 *
DATABASE EMEST9 EMBL Heidelberg, Germany; 8 February 1999 (1999-02-08), WALBOT V: "Maize ESTs from various cDNA libraries sequenced at Stanford University", XP002141583 *
DATABASE WPI Week 199608, Derwent World Patents Index; AN 1996-072337, XP002141585, "DNA sequence encoding cotton UDP glucose synthase" *
EIMERT K ET AL.: "Cloning and characterization of several cDNAs for UDP-glucose pyrophosphorylase from barley (Hordeum vulgare) tissues", GENE, vol. 170, 1996, pages 227 - 232, XP002141582 *
KATSUBE T ET AL.: "Expression in Escherichia coli of UDP-glucose pyrophosphorylase cDNA from potato tuber and functional assessment of the five lysyl residues located at the substrate binding site", BIOCHEMISTRY, vol. 30, 1991, pages 8546 - 8551, XP002141581 *
OZBUN J L ET AL.: "Starch synthetase, phosphorylase, ADPglucose pyrophosphorylase, and UDPglucose pyrophosphorylase in developing maize kernels", PLANT PHYSIOLOGY, vol. 51, 1973, pages 1 - 5, XP000915079 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103725658A (en) * 2013-12-23 2014-04-16 内蒙古大学 Dahurian-larch-derived plant-development-related protein LgUGPase, and coding gene and application thereof
CN103725658B (en) * 2013-12-23 2015-11-18 内蒙古大学 Derive from the plant development associated protein LgUGPase of larch in Xinanlin area and encoding gene thereof and application

Also Published As

Publication number Publication date
EP1151111A2 (en) 2001-11-07
EP1151115A2 (en) 2001-11-07
AU3227300A (en) 2000-08-29
CA2359811A1 (en) 2000-08-17
WO2000047755A3 (en) 2001-03-08
CA2716429A1 (en) 2000-08-17
CA2359811C (en) 2010-12-21
CA2359803A1 (en) 2000-08-17
AR022567A1 (en) 2002-09-04
WO2000047755A2 (en) 2000-08-17
CA2359803C (en) 2013-04-09
AU3228300A (en) 2000-08-29
WO2000047748A2 (en) 2000-08-17

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