WO2001046393A3 - Aspartate kinase - Google Patents

Aspartate kinase Download PDF

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Publication number
WO2001046393A3
WO2001046393A3 PCT/US2000/034396 US0034396W WO0146393A3 WO 2001046393 A3 WO2001046393 A3 WO 2001046393A3 US 0034396 W US0034396 W US 0034396W WO 0146393 A3 WO0146393 A3 WO 0146393A3
Authority
WO
WIPO (PCT)
Prior art keywords
aspartate kinase
relates
chimeric gene
aspartate
kinase
Prior art date
Application number
PCT/US2000/034396
Other languages
French (fr)
Other versions
WO2001046393A2 (en
Inventor
Saverio Carl Falco
Omolayo O Famodu
Catherine J Thorpe
Original Assignee
Du Pont
Saverio Carl Falco
Omolayo O Famodu
Catherine J Thorpe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Saverio Carl Falco, Omolayo O Famodu, Catherine J Thorpe filed Critical Du Pont
Priority to AU22767/01A priority Critical patent/AU2276701A/en
Priority to US09/890,813 priority patent/US6861575B2/en
Priority to EP00986545A priority patent/EP1203085A2/en
Publication of WO2001046393A2 publication Critical patent/WO2001046393A2/en
Publication of WO2001046393A3 publication Critical patent/WO2001046393A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8253—Methionine or cysteine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine

Abstract

This invention relates to an isolated nucleic acid fragment encoding an aspartate kinase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the aspartate kinase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the aspartate kinase in a transformed host cell.
PCT/US2000/034396 1999-12-21 2000-12-19 Aspartate kinase WO2001046393A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU22767/01A AU2276701A (en) 1999-12-21 2000-12-19 Aspartate kinase
US09/890,813 US6861575B2 (en) 1999-12-21 2000-12-19 Aspartate kinase
EP00986545A EP1203085A2 (en) 1999-12-21 2000-12-19 Aspartate kinase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US17294499P 1999-12-21 1999-12-21
US60/172,944 1999-12-21

Publications (2)

Publication Number Publication Date
WO2001046393A2 WO2001046393A2 (en) 2001-06-28
WO2001046393A3 true WO2001046393A3 (en) 2002-02-14

Family

ID=22629853

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/034396 WO2001046393A2 (en) 1999-12-21 2000-12-19 Aspartate kinase

Country Status (4)

Country Link
US (4) US6861575B2 (en)
EP (1) EP1203085A2 (en)
AU (1) AU2276701A (en)
WO (1) WO2001046393A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0304860A (en) * 2002-11-11 2004-08-31 Ajinomoto Kk Method for producing a target substance by use of a bacterium belonging to the genus Escherichia
ES2560449T3 (en) 2004-05-28 2016-02-19 Oryxe A mixture for transdermal administration of low and high molecular weight compounds
WO2017048807A1 (en) 2015-09-17 2017-03-23 Jrx Biotechnology, Inc. Approaches for improving skin hydration or moisturization

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451516A (en) * 1991-08-16 1995-09-19 Us Agriculture Bifunctional protein from carrots (Daucus carota) with aspartokinase and homoserine dehydrogenase activities

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5258300A (en) * 1988-06-09 1993-11-02 Molecular Genetics Research And Development Limited Partnership Method of inducing lysine overproduction in plants
EP0485970A3 (en) 1990-11-13 1992-07-01 Yeda Research And Development Company Limited Transgenic plants overproducing threonine and lysine
US5773691A (en) * 1992-03-19 1998-06-30 E. I. Du Pont De Nemours And Company Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants
US5850016A (en) * 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451516A (en) * 1991-08-16 1995-09-19 Us Agriculture Bifunctional protein from carrots (Daucus carota) with aspartokinase and homoserine dehydrogenase activities

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ESAU ET AL.: "Glycine max precursor monofunctional aspartokinase mRNA, complete cds.", EMBL SEQUENCE DATABASE, 1 July 1999 (1999-07-01), HEIDELBERG DE, XP002168270 *
ESAU ET AL.: "Precursor monofunctional aspartokinase", SWISSPROT SEQUENCE DATA BASE, 1 November 1999 (1999-11-01), XP002168271 *
FRANKARD ET AL.: "A. thaliana mRNA for aspartate kinase", EMBL SEQUENCE DATABASE, 2 July 1997 (1997-07-02), HEIDELBERG DE, XP002168264 *
FRANKARD ET AL.: "Aspartate kinase precursor (EC 2.7.2.4)", SWISSPROT SEQUENCE DATA BASE, 1 January 1998 (1998-01-01), XP002168265 *
FRANKARD ET AL.: "Molecular characterization of an Arabidopsis thaliana cDNA coding for a monofunctional aspartate kinase", PLANT MOL. BIOL., vol. 34, 1997, pages 233 - 242, XP001002447 *
KARCHI H ET AL: "SEED-SPECIFIC EXPRESSION OF A BACTERIAL DESENSITIZED ASPARTATE KINASE INCRASES THE PRODUCTION OF SEED THREONINE AND METHIONINE IN TRANSGENIC TOBACCO", PLANT JOURNAL,GB,BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, vol. 3, no. 5, 1993, pages 721 - 727, XP002015695, ISSN: 0960-7412 *
KIYOTA: "Aspartate kinase (fragment)", SWISSPROT SEQUENCE DATA BASE, 1 October 2000 (2000-10-01), XP002168273 *
KIYOTA: "Oryza stiva mRNA for aspartate kinase, partial cds.", EMBL SEQUENCE DATABASE, 12 May 2000 (2000-05-12), HEIDELBERG DE, XP002168272 *
TANG ET AL.: "Arabidopsis thaliana lysine-sensitive aspartate kinase mRNA, complete cds.", EMBL SEQUENCE DATABASE, vol. 34, no. 2, 1997, HEIDELBERG DE, pages 287 - 294, XP002168267 *
TANG ET AL.: "Cloning and expression of an Arabidopsis thaliane cDNA encoding a monofunctional aspartate kinase homologous to the lysine-sensitive enzyme of Escherischia coli", PLANT MOL. BIOL., vol. 34, no. 2, 1997, pages 287 - 294, XP001002446 *
TANG ET AL.: "Lysine-sensitive aspartate kinase", SWISSPROT SEQUENCE DATA BASE, 1 January 1998 (1998-01-01), XP002168268 *

Also Published As

Publication number Publication date
US20050251876A1 (en) 2005-11-10
AU2276701A (en) 2001-07-03
WO2001046393A2 (en) 2001-06-28
EP1203085A2 (en) 2002-05-08
US6861575B2 (en) 2005-03-01
US20020183486A1 (en) 2002-12-05
US20080178347A1 (en) 2008-07-24
US20060156441A1 (en) 2006-07-13
US7227054B2 (en) 2007-06-05
US7345220B2 (en) 2008-03-18
US7723572B2 (en) 2010-05-25

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