WO2001014540A3 - Starch synthase isoform v - Google Patents

Starch synthase isoform v Download PDF

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Publication number
WO2001014540A3
WO2001014540A3 PCT/US2000/023231 US0023231W WO0114540A3 WO 2001014540 A3 WO2001014540 A3 WO 2001014540A3 US 0023231 W US0023231 W US 0023231W WO 0114540 A3 WO0114540 A3 WO 0114540A3
Authority
WO
WIPO (PCT)
Prior art keywords
starch synthase
synthase isoform
isoform
relates
chimeric gene
Prior art date
Application number
PCT/US2000/023231
Other languages
French (fr)
Other versions
WO2001014540A2 (en
Inventor
Stephen M Allen
Karen E Broglie
Catherine J Thorpe
Original Assignee
Du Pont
Stephen M Allen
Karen E Broglie
Catherine J Thorpe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Stephen M Allen, Karen E Broglie, Catherine J Thorpe filed Critical Du Pont
Priority to AU70685/00A priority Critical patent/AU7068500A/en
Publication of WO2001014540A2 publication Critical patent/WO2001014540A2/en
Publication of WO2001014540A3 publication Critical patent/WO2001014540A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

This invention relates to an isolated nucleic acid fragment encoding a starch synthase isoform V. The invention also relates to the construction of a chimeric gene encoding all or a portion of the starch synthase isoform V, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the starch synthase isoform V in a transformed host cell.
PCT/US2000/023231 1999-08-25 2000-08-24 Starch synthase isoform v WO2001014540A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU70685/00A AU7068500A (en) 1999-08-25 2000-08-24 Starch synthase isoform v

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15057999P 1999-08-25 1999-08-25
US60/150,579 1999-08-25

Publications (2)

Publication Number Publication Date
WO2001014540A2 WO2001014540A2 (en) 2001-03-01
WO2001014540A3 true WO2001014540A3 (en) 2001-12-06

Family

ID=22535157

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/023231 WO2001014540A2 (en) 1999-08-25 2000-08-24 Starch synthase isoform v

Country Status (2)

Country Link
AU (1) AU7068500A (en)
WO (1) WO2001014540A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6849781B2 (en) 2001-06-08 2005-02-01 E. I. Du Pont De Nemours And Company Starch synthase isoform V
EP1882742A1 (en) * 2006-07-28 2008-01-30 Institut National De La Recherche Agronomique (Inra) Plants defective for soluble starch synthase IV (SSIV) activity, methods for obtaining the same, and uses thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997026362A1 (en) * 1996-01-16 1997-07-24 Planttec Biotechnologie Gmbh Nucleic acid molecules from plants coding enzymes which participate in the starch synthesis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997026362A1 (en) * 1996-01-16 1997-07-24 Planttec Biotechnologie Gmbh Nucleic acid molecules from plants coding enzymes which participate in the starch synthesis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 1 April 1999 (1999-04-01), BHULLAR SS: "Vigna unguiculata mRNA for starch synthase, isoform V", XP002157769 *
KOSSMANN J ET AL: "Cloning and functional analysis of a cDNA encoding a starch synthase from potato (Solanum tuberosum L.) that is predominantly expressed in leaf tissue.", PLANTA, vol. 208, no. 4, June 1999 (1999-06-01), pages 503 - 511, XP000973305 *

Also Published As

Publication number Publication date
WO2001014540A2 (en) 2001-03-01
AU7068500A (en) 2001-03-19

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