Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
  • A61K31/57—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
  • A61K9/0007—Effervescent
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0012—Galenical forms characterised by the site of application
  • A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/02—Suppositories; Bougies; Bases therefor; Ovules
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/20—Pills, tablets, discs, rods
  • A61K9/2004—Excipients; Inactive ingredients
  • A61K9/2022—Organic macromolecular compounds
  • A61K9/2027—Organic macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyvinyl pyrrolidone, poly(meth)acrylates
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/20—Pills, tablets, discs, rods
  • A61K9/2095—Tabletting processes; Dosage units made by direct compression of powders or specially processed granules, by eliminating solvents, by melt-extrusion, by injection molding, by 3D printing
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
  • A61P15/12—Drugs for genital or sexual disorders; Contraceptives for climacteric disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P5/00—Drugs for disorders of the endocrine system
  • A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P5/00—Drugs for disorders of the endocrine system
  • A61P5/24—Drugs for disorders of the endocrine system of the sex hormones
  • A61P5/34—Gestagens
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/20—Pills, tablets, discs, rods
  • A61K9/2004—Excipients; Inactive ingredients
  • A61K9/2009—Inorganic compounds
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/20—Pills, tablets, discs, rods
  • A61K9/2004—Excipients; Inactive ingredients
  • A61K9/2013—Organic compounds, e.g. phospholipids, fats
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/20—Pills, tablets, discs, rods
  • A61K9/2004—Excipients; Inactive ingredients
  • A61K9/2013—Organic compounds, e.g. phospholipids, fats
  • A61K9/2018—Sugars, or sugar alcohols, e.g. lactose, mannitol; Derivatives thereof, e.g. polysorbates

Definitions

• the invention relates to the preparation of pharmaceutical compositions containing progesterone, in particular to compositions for vaginal delivery of progesterone.
• HRT hormonal replacement therapy
• Natural progesterone is devoid of any androgenic activity that might compromise lipoprotein metabolism or induce teratogenicity. Moreover, it probably has a direct beneficial effect on blood vessels (Jiang et al., Eur. J. Pharmacol. 211
• Intramuscular injection assures reliable absorption, but is painful, can cause local irritation and cold abscesses (Devroey et al , Int J Fertil 34 (1989), 188-193). must be administered by trained medical personnel, and often suffers from low patient compliance For these reasons, the vaginal route has become the most established way in which to deliver natural progesterone The progesterone is easily administered to the vagina, which has a large potential of absorption, and also avoids liver first-pass metabolism when delivered to the vagina
• vaginal formulations have been assayed, mostly as suppositories (P ⁇ ce et al , Fertil Steril 39 (1983), 490-493, Norman et al , Fertil Steril 56 (1991), 1034- 1039, Archer et al , Am J Obstet Gynecol , 173 (1995), 471-478), gelatin capsules (Devroey et al , Int J Fertil 34 (1989), 188-193, Smitz et al , Hum Reprod 2 (1992), 309-314, Miles et al , Fertil Steril 62 (1994), 485-490), and recently as bio-adhesive gels (Fanchin et al , Obstet Gynecol 90 (1997), 396-401 , Ross et al , Am J Obstet Gynecol 177 (1997), 937-941)
• progesterone employs a wet granulation technique because commercially available progesterone has bulk properties which render it unsuitable for direct compaction in the concentrations necessary for use in ART (typically about 50-100 mg progesterone per 1000 mg tablet). Greco gives no suggestion as to how one might be able to tablet progesterone via a direct-compaction method, which is economically more desirable.
• the present invention seeks to provide a method for the production of a tablet for the vaginal delivery of progesterone as well as tablets containing progesterone.
• a method for preparing a tablet for the vaginal administration of progesterone for systemic use comprising the steps of: slowly mixing water with micronized progesterone, the total amount of water mixed with said micronized progesterone not exceeding the maximum wetting capacity of the micronized progesterone, whereby to obtain wetted micronized progesterone; drying said wetted micronized progesterone to a humidity content of substantially 0%, whereby to form substantially dry micronized progesterone; mixing said substantially dry micronized progesterone with other pharmaceutically acceptable excipients or diluents therefor; and forming a tablet by direct compaction of said substantially dry micronized progesterone which has been mixed with said other pharmaceutically acceptable excipients or diluents therefor.
• a method for preparing a tablet for the vaginal administration of progesterone for systemic use comprising the steps of: slowly mixing water with micronized progesterone, the total amount of water mixed with said micronized progesterone not exceeding the maximum wetting capacity of the micronized progesterone, whereby to obtain wetted micronized progesterone; drying said wetted micronized progesterone to a humidity content of substantially 0%, whereby to form substantially dry micronized progesterone; mixing said substantially dry micronized progesterone with other pharmaceutically acceptable excipients or diluents therefor, including an effervescent; and forming a tablet by direct compaction of said substantially dry micronized progesterone which has been mixed with said other pharmaceutically acceptable excipients or diluents therefor, including an effervescent.
• a method for preparing a tablet for the vaginal administration of progesterone for systemic use comprising the steps of: slowly mixing water with micronized progesterone, the total amount of water mixed with said micronized progesterone does not exceed the maximum wetting capacity of the amount of micronized progesterone, whereby to obtain wetted micronized progesterone; drying said wetted micronized progesterone to a humidity content of substantially 0%, whereby to form substantially dry micronized progesterone; sieving a first lubricant to obtain a sieved first lubricant; mixing said substantially dry micronized progesterone with said sieved first lubricant and a material selected from a first filler or a disintegrant to form a first mixture; mixing a binder which binds dry particles with said first mixture to form a second mixture; intimately mixing an effervescent and a first quantity of a second filler to
• the amount of water mixed with the micronized progesterone is between about 25 and 28 wt % of the amount of micronized progesterone
• the water is added to the micronized progesterone at rate of between about 6-9 ml per minute, at a mixing speed of between about 25-33 3 rpm
• the first lub ⁇ cant is sieved through sieves having a pore size of between about 400 and 450 microns, preferably about 425 microns
• the third mixture is sieved through sieves having a pore size of between about 400 and 450 microns, preferably about 425 microns prior to mixing with the second mixture
• said sieved second lubricant and said sieved third lub ⁇ cant are sieved through sieves having a pore size of between about 100 and 150 microns, preferably 125 microns prior to mixing with said fifth mixture.
• said drying of said wetted micronized progesterone is done at a temperature of between about 55°C and about 60°C.
• all of said mixing steps are carried out at a temperature of between about 15°C and 30°C.
• said first lubricant is silicon dioxide (colloidal anhydrous silica).
• said material selected from a first filler or a disintegrant is a starch exhibiting good flow properties, such as cornstarch 1500 or other starches derived from corn (maize), potatoes or wheat, as are well known in the art.
• the binder which binds dry particles is polyvinylpyrrolidone (povidone), e.g. Povidone 30.
• said second filler is derived from a natural source and is more preferably lactose or is composed principally of lactose (e.g. ludipress, which as is well known in the art is a commercially available mixture of polyvinylpyrrolidone and lactose).
• lactose e.g. ludipress, which as is well known in the art is a commercially available mixture of polyvinylpyrrolidone and lactose.
• said effervescent is a mixture of a pharmaceutically acceptable carboxylic or dicarboxylic acid, such as adipic acid or tartaric acid, and a pharmaceutically acceptable salt of HCO , such as sodium bicarbonate.
• a pharmaceutically acceptable carboxylic or dicarboxylic acid such as adipic acid or tartaric acid
• a pharmaceutically acceptable salt of HCO such as sodium bicarbonate.
• the acid and bicarbonate are present in an amount providing a molar excess of-COOH groups.
• said first portion and said second portion of said second filler are of generally the same size
• the effervescent is prepared prior to said intimate mixing of said first portion of said second filler with said effervescent In another preferred embodiment of the invention, said effervescent is prepared in situ as part of said intimate mixing of said first portion of said second filler with said effervescent
• said intimate mixing of said first portion of said second filler with said effervescent comprises non-intimately mixing said first portion of said second filler with said effervescent and passing the resultant non-intimately mixed mixture through a sieve having an average pore size between about 400 and 450 microns, preferably about 425 microns diameter to obtain said third mixture
• the effervescent comprises between about 6 and 10 wt %, preferably about 8 wt % of the tablet
• said intimate mixing of said second mixture with said third mixture to obtain said fourth mixture is accomplished by non-intimately mixing said second mixture with said third mixture to obtain a non- intimately mixed mixture and sifting said non-intimately mixed mixture through a sieve having an average pore size between about 400 and 450 microns, preferably about 425 microns diameter to obtain said fourth mixture
• said second lubricant is selected from magnesium stearate, talc, sodium lauryl sulfate, and phosphates known in the art to function as lub ⁇ cants
• said material selected from a saponificant or a third lubricant is sodium lauryl sulfate.
• a tablet prepared by the steps of: slowly mixing water with micronized progesterone, the total amount of water mixed -with said micronized progesterone not exceeding the maximum wetting capacity of the amount of micronized progesterone, whereby to obtain wetted micronized progesterone; drying said wetted micronized progesterone to a humidity content of substantially 0%, whereby to form substantially dry micronized progesterone; mixing said substantially dry micronized progesterone with other pharmaceutically acceptable excipients or diluents therefor; and forming a tablet by direct compaction of said substantially dry micronized progesterone which has been mixed with said other pharmaceutically acceptable excipients or diluents therefor.
• a tablet prepared by the steps of: slowly mixing water with micronized progesterone, the total amount of water mixed with said micronized progesterone not exceeding the maximum wetting capacity of the amount of micronized progesterone, whereby to obtain wetted micronized progesterone; drying said wetted micronized progesterone to a humidity content of substantially 0%, whereby to form substantially dry micronized progesterone; mixing said substantially dry micronized progesterone with other pharmaceutically acceptable excipients or diluents therefor, including an effervescent; and forming a tablet by direct compaction of said substantially dry micronized progesterone which has been mixed with said other pharmaceutically acceptable excipients or diluents therefor, including an effervescent.
• a tablet prepared by the steps of: slowly mixing water with micronized progesterone, the total amount of water mixed with said micronized progesterone not exceeding the maximum wetting capacity of the amount of micronized progesterone, whereby to obtain wetted micronized progesterone, drying said wetted micronized progesterone to a humidity content of substantially 0%, whereby to form substantially dry micronized progesterone, sieving a first lubricant to obtain a sieved first lub ⁇ cant, mixing said substantially dry micronized progesterone with said sieved first lub ⁇ cant and a mate ⁇ al selected from a first filler or a disintegrant to form a first mixture, mixing a binder which binds dry particles with said first mixture to form a second mixture, intimately mixing an effervescent and a first quantity of a second filler to form a third mixture, sieving said third mixture to obtain a sieved third mixture
• a tablet comprising between about 6 to 20 wt % progesterone and between about 5 to 12 wt % effervescent
• the tablet comp ⁇ ses between about 8 to 12 wt % progesterone
• the tablet comprises between about 6 to 8 wt % effervescent
• Example 1 Preparation of Tablets Step 1 To 1000 g of micronized progesterone were added 280 g of distilled water, with mixing using a planetary mixer, over a pe ⁇ od of 30 minutes After mixing, the wetted micronized progesterone was spread on pans to thickness of about
• step 2 the dried micronized progesterone was either used immediately in step 2 as described below, or was stored in dry, sealed containers for later use in step 2.
• Step 2 Colloidal anhydrous silica (Aerosil 380, 25 g) was sieved through a Russel sieve having pores of 425 micron size, and mixed for 10 minutes with 1000 g of micronized progesterone from Step 1 and 2100 g of maize 1500 starch, using an Angelsman mixer at 32 RPM, to form Mixture A. At the end of the 10 minutes of mixing, 490 g of povidone 30 were added to Mixture A, and mixing was continued for another ten minutes, to prepare "Mixture B".
• Step 3 Lactose (Ludipress, BASF, 3800 g), adipic acid (570 g) and sodium bicarbonate (430 g) were mixed for 10 minutes at room temperature using an Angelsman mixer at 32 RPM. Following mixing, these ingredients were sieved through a Russel sieve having pores of 425 microns to obtain "Mixture C”.
• Step 4 Mixtures B and C were mixed for 10 minutes at room temperature using an Angelsman mixer at 32 RPM to obtain "Mixture D”.
• Step 5 Mixture D (8415 g) was mixed with 3800 g of lactose (Ludipress) for 10 minutes at room temperature using an Angelsman mixer at 32 RPM, to obtain "Mixture E”.
• Step 6 Magnesium stearate (230 g) and sodium lauryl sulfate (50 g) were sieved through a Russel sieve (pore size 125 microns). The sieved magnesium stearate and sodium lauryl sulfate were then mixed for with mixture E for 20 minutes at room temperature using an Angelsman mixer, to obtain "Mixture F”.
• Step 7 Tablets were obtained from mixture F by direct compaction using an Eko Korsch Press. The amounts of ingredients listed in this example are suitable for production of 10,000 tablets each containing about 100 mg progesterone.
• Example 3 The process described in Example 1 was modified by doubling the amount of filler (Ludipress) to obtain tablets containing on average 50 mg progesterone.
• Example 4 The pharmacokinetics and clinical use of tablets prepared in accordance with the invention were evaluated as follows: 50 healthy, post-menopausal women with intact uteri, 39 of whom had suffered premature menopause and 1 1 who were truly postmenopausal, all of whom were undergoing hormone replacement therapy (HRT), submitted blood samples for determination of baseline profiles of hormones (progesterone and other hormones) and other biochemicals (bilirubin, cholesterol, etc.). The blood samples were taken at 8 AM on the first day of the evaluation (day 0) in a fasting state, by intravenous indwelling catheter which was introduced into the cubital vein.
• HRT hormone replacement therapy
• Non-estrogen primed postmenopausal women were chosen in order to avoid confusion with endogenous progesterone secretion and estrogen influence on vaginal mucosa absorption (Villanueva et al., Fertil. Steril. 35 (1981), 433-437).
• Body mass index was calculated as weight in kg divided by the square of height in meters.
• a single vaginal application of a 50 mg progesterone-containing tablet prepared in accordance with the invention resulted in the rapid increase of plasma progesterone concentration.
• the mean peak plasma level (T max ), mean elimination half-life (T 1 2 ), maximal serum concentration (C ma *), and AUC (area under the curve, i.e. total amount of plasma progesterone observed) derived from the blood samples taken on day 0 of the evaluation are summarized in Table II. Table II
• Part B Single-dose pharmacokmetics of micronized progesterone the form of a vaginal tablet according to the present invention After a washout period of 2 weeks, the same subjects as in Part A were again administered 4 mg or ethinyl estradiol (Estrofem) for 14 days On day 14 the same procedure as recited in Part A was repeated, except that this time the women were instructed to insert 100 mg of progesterone in the form of an effervescent tablet according to the present invention, using a plastic applicator Blood samples for progesterone levels were drawn at the same intervals as in Part A
• Table IV summarizes the baseline details of the of the thirteen women who participated in the study of Example 5 Table IV
• Body mass index was calculated as weight in kg divided by the square of height in meters
• T ma mean elimination half-life (T ) 2
• C md maximal serum concentration
• AUC area under the curve, i.e. total amount of plasma progesterone observed
• a single dose of 100 mg micronized progesterone in the form of both gelatin capsules and vaginally administrable tablets in accordance with the present invention resulted in a similar rapid increase in plasma progesterone levels within 2.5-3 hours after administration.
• the statistically significant difference of variance between the two groups indicates a more predictable T, ⁇ for the tablets of the present invention than for the prior art gelatin capsules.
• the amounts and proportions of ingredients recited in the foregoing examples are illustrative only, and that these amounts and proportions may be varied within the scope of the invention.
• the Example 1 the amount of effervescent recited is about 8 wt.% of the tablets which are the final product of the process described in Example 1.
• the effervescent may be omitted in the practice of the invention, or it may be included in an amount of up to about 12 wt.% of the tablet.
• the effervescent constitutes between about 5-12 wt.%, more preferably between about 6-8 wt.% of the tablet.
• progesterone may constitute up to about 20 wt.% of the tablet, preferably between about 6-20 wt.%, more preferably between about 8-12 wt.% of the tablet. It will be appreciated that various features of the invention which are, for clarity, described in the contexts of separate embodiments may also be provided in combination in a single embodiment. Conversely, various features of the invention which are, for brevity, described in the context of a single embodiment may also be provided separately or in any suitable subcombination.

Landscapes

• Health & Medical Sciences (AREA)
• Public Health (AREA)
• Animal Behavior & Ethology (AREA)
• Pharmacology & Pharmacy (AREA)
• Veterinary Medicine (AREA)
• Chemical & Material Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Medicinal Chemistry (AREA)
• Epidemiology (AREA)
• Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Endocrinology (AREA)
• Organic Chemistry (AREA)
• Chemical Kinetics & Catalysis (AREA)
• General Chemical & Material Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Reproductive Health (AREA)
• Diabetes (AREA)
• Gynecology & Obstetrics (AREA)
• Urology & Nephrology (AREA)
• Medicinal Preparation (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Priority Applications (11)

Applications Claiming Priority (2)

Related Child Applications (1)

Publications (1)

Family

ID=11072154

Family Applications (1)

Country Status (8)

Cited By (2)

Families Citing this family (9)

Citations (2)

Family Cites Families (23)

• 1998
  • 1998-11-18 IL IL12712998A patent/IL127129A/en not_active IP Right Cessation
• 1999
  • 1999-11-17 DK DK99972096.4T patent/DK1131052T3/da active
  • 1999-11-17 AU AU11762/00A patent/AU1176200A/en not_active Abandoned
  • 1999-11-17 WO PCT/IL1999/000619 patent/WO2000028970A1/en not_active Ceased
  • 1999-11-17 EP EP11166346A patent/EP2386291A1/en not_active Ceased
  • 1999-11-17 US US09/856,417 patent/US7300664B1/en not_active Expired - Lifetime
  • 1999-11-17 EP EP99972096A patent/EP1131052B1/en not_active Expired - Lifetime
  • 1999-11-17 ES ES99972096T patent/ES2390959T3/es not_active Expired - Lifetime
  • 1999-11-17 JP JP2000582018A patent/JP4667601B2/ja not_active Expired - Lifetime
• 2004
  • 2004-04-26 US US10/832,742 patent/US7393543B2/en not_active Expired - Lifetime
• 2005
  • 2005-01-12 US US11/039,062 patent/US7320800B2/en not_active Expired - Lifetime
• 2006
  • 2006-04-20 US US11/408,614 patent/US20060188571A1/en not_active Abandoned
• 2010
  • 2010-10-01 JP JP2010223566A patent/JP2011026335A/ja active Pending

Patent Citations (2)

Non-Patent Citations (2)

Also Published As

Similar Documents

Legal Events