WO2000023597A3 - Tissue-specific promoters for gene expression - Google Patents

Tissue-specific promoters for gene expression Download PDF

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Publication number
WO2000023597A3
WO2000023597A3 PCT/GB1999/003442 GB9903442W WO0023597A3 WO 2000023597 A3 WO2000023597 A3 WO 2000023597A3 GB 9903442 W GB9903442 W GB 9903442W WO 0023597 A3 WO0023597 A3 WO 0023597A3
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WO
WIPO (PCT)
Prior art keywords
tissue
expression
gene expression
localised
specific promoters
Prior art date
Application number
PCT/GB1999/003442
Other languages
French (fr)
Other versions
WO2000023597A2 (en
Inventor
Gordon Cameron Machray
Diane Davidson
Peter Edward Hedley
Original Assignee
Scottish Crop Research Inst
Gordon Cameron Machray
Diane Davidson
Peter Edward Hedley
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9822490.0A external-priority patent/GB9822490D0/en
Priority claimed from GBGB9823481.8A external-priority patent/GB9823481D0/en
Application filed by Scottish Crop Research Inst, Gordon Cameron Machray, Diane Davidson, Peter Edward Hedley filed Critical Scottish Crop Research Inst
Priority to AU62215/99A priority Critical patent/AU6221599A/en
Publication of WO2000023597A2 publication Critical patent/WO2000023597A2/en
Publication of WO2000023597A3 publication Critical patent/WO2000023597A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8229—Meristem-specific, e.g. nodal, apical

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Expression control polynucleotides of an invertase gene are described which are tissue specific to vascular tissue and/or to nodal tissue. The expression control polynucleotides may be operably linked to a heterologous polynucleotide, for example in the form of a vector and/or used to produce transgenic plants. The heterologous polynucleotide may express polypeptides or proteins which are insecticidal or which promote or inhibit plant growth and expression thereof will be localised to the vascular tissue. Where the transgenic plant is a potato plant, stolon formation and/or tuber sprouting may be likewise controlled through localised expression of suitable heterologous gene products in nodal tissue.
PCT/GB1999/003442 1998-10-16 1999-10-15 Tissue-specific promoters for gene expression WO2000023597A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU62215/99A AU6221599A (en) 1998-10-16 1999-10-15 Tissue-specific promoters for gene expression

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB9822490.0 1998-10-16
GBGB9822490.0A GB9822490D0 (en) 1998-10-16 1998-10-16 Nucleic acid
GB9823481.8 1998-10-28
GBGB9823481.8A GB9823481D0 (en) 1998-10-28 1998-10-28 Nucleic acid

Publications (2)

Publication Number Publication Date
WO2000023597A2 WO2000023597A2 (en) 2000-04-27
WO2000023597A3 true WO2000023597A3 (en) 2000-07-27

Family

ID=26314519

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/003442 WO2000023597A2 (en) 1998-10-16 1999-10-15 Tissue-specific promoters for gene expression

Country Status (2)

Country Link
AU (1) AU6221599A (en)
WO (1) WO2000023597A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7365186B2 (en) 2002-11-22 2008-04-29 Arborgen, Llc Vascular-preferred promoter sequences and uses thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993004177A1 (en) * 1991-08-27 1993-03-04 Agricultural Genetics Company Limited Proteins with insecticidal properties against homopteran insects and their use in plant protection
DE4213444A1 (en) * 1992-04-18 1993-10-28 Inst Genbiologische Forschung Prodn. of potato plants with suppressed tuber sprouting - by genetic modification to reduce sucrose concn.
WO1997044471A2 (en) * 1996-05-17 1997-11-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Potato plants with reduced cytosolic starch phosphorylasis and modified germination
WO1998011240A2 (en) * 1996-09-09 1998-03-19 B.C. Research Inc. A process of increasing plant growth and yield and modifiying cellulose production in plants
WO1998041643A1 (en) * 1997-03-19 1998-09-24 Scottish Crop Research Institute Expression control polynucleotides
WO1999006578A2 (en) * 1997-07-30 1999-02-11 Zeneca Limited Genetic method for controlling sprouting
DE19734218A1 (en) * 1997-08-07 1999-02-11 Max Planck Gesellschaft Processes for increasing yield in plants

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993004177A1 (en) * 1991-08-27 1993-03-04 Agricultural Genetics Company Limited Proteins with insecticidal properties against homopteran insects and their use in plant protection
DE4213444A1 (en) * 1992-04-18 1993-10-28 Inst Genbiologische Forschung Prodn. of potato plants with suppressed tuber sprouting - by genetic modification to reduce sucrose concn.
WO1997044471A2 (en) * 1996-05-17 1997-11-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Potato plants with reduced cytosolic starch phosphorylasis and modified germination
WO1998011240A2 (en) * 1996-09-09 1998-03-19 B.C. Research Inc. A process of increasing plant growth and yield and modifiying cellulose production in plants
WO1998041643A1 (en) * 1997-03-19 1998-09-24 Scottish Crop Research Institute Expression control polynucleotides
WO1999006578A2 (en) * 1997-07-30 1999-02-11 Zeneca Limited Genetic method for controlling sprouting
DE19734218A1 (en) * 1997-08-07 1999-02-11 Max Planck Gesellschaft Processes for increasing yield in plants

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1995, ASTHIR BAVITA ET AL: "Invertase-mediated interconversion of sucrose and hexoses during their translocation in growing pearl millet plant.", XP002135167, Database accession no. PREV199598366192 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1996, TYMOWSKA-LALANNE ZUZANNA ET AL: "Expression and cis-acting elements of the At-beta-fruct1 gene from Arabidopsis thaliana encoding a cell wall invertase.", XP002135166, Database accession no. PREV199699006068 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, IVANA MACHACKOVA ET AL: "Growth pattern, tuber formation and hormonal balance in in vitro potato plants carrying ipt gene.", XP002135168, Database accession no. PREV199799555659 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, ZHANG LONG ET AL: "A pea cell-wall invertase gene (PsInv-1) with tissue-specific expression.", XP002135165, Database accession no. PREV199799814349 *
FU HONGYONG ET AL: "High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 5' and 3' flanking sequences and the leader intron.", PLANT CELL 1995, vol. 7, no. 9, 1995, pages 1387 - 1394, XP002135161, ISSN: 1040-4651 *
FU, H., ET AL.: "A potato Sus3 sucrose synthase gene contains a context-dependent 3' element and a leader intron with both positive and negative tissue-specific effects", THE PLANT CELL, vol. 7, no. 9, September 1995 (1995-09-01), pages 1395 - 1403, XP002135162 *
HEDLEY PETER E ET AL: "Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression.", GENE (AMSTERDAM) 1994, vol. 145, no. 2, 1994, pages 211 - 214, XP002071296, ISSN: 0378-1119 *
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 1995, vol. 4, no. 1, 1995, pages 23 - 28 *
LERCHL ET AL: "IMPAIRED PHOTOASSIMILATE PARTITIONING CAUSED BY PHLOEM-SPECIFIC REMOVAL OF PYROPHOSPHATE CAN BE COMPLEMENTED BY A PHLOEM-SPECIFIC CYTOSOLIC YEAST-DERIVED INVERTASE IN TRANSGENIC PLANTS", PLANT CELL,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 7, March 1995 (1995-03-01), pages 259 - 270, XP002086882, ISSN: 1040-4651 *
MADDISON, A.L., ET AL.: "Expression of tandem invertase genes associated with sexual and vegetative growth cycles in potato", PLANT MOLECULAR BIOLOGY, vol. 41, December 1999 (1999-12-01), pages 741 - 751, XP002135164 *
MEYER R ET AL: "Promoter deletion analysis of potato invertase gene expression", JOURNAL OF EXPERIMENTAL BOTANY,GB,OXFORD UNIVERSITY PRESS, vol. 45, no. SUPPL, 11 April 1994 (1994-04-11), XP002071293, ISSN: 0022-0957 *
OHTA S ET AL: "HIGH-LEVEL EXPRESSION OF A SWEET POTATO SPORAMIN GENE PROMOTER BETA GLUCURONIDASE GUS FUSION GENE IN THE STEMS OF TRANSGENIC TOBACCO PLANTS IS CONFERRED BY MULTIPLE CELL TYPE-SPECIFIC REGULATORY ELEMENTS", MOLECULAR & GENERAL GENETICS 1991, vol. 225, no. 3, 1991, pages 369 - 378, XP002135160, ISSN: 0026-8925 *
OOSUMI, T., ET AL.: "Solanum chacoense transposable element Sch2, sequence.", EMBL ACCESSION NO:U91993, 8 April 1997 (1997-04-08), XP002135188 *
PLANT GROWTH REGULATION 1997, vol. 21, no. 1, 1997, pages 27 - 36, ISSN: 0167-6903 *
PLANT PHYSIOLOGY AND BIOCHEMISTRY (PARIS) 1996, vol. 34, no. 3, 1996, pages 431 - 442, ISSN: 0981-9428 *
PLANT PHYSIOLOGY AND BIOCHEMISTRY (PARIS) 1997, vol. 35, no. 10, 1997, pages 751 - 760, ISSN: 0981-9428 *

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AU6221599A (en) 2000-05-08

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