WO2000023597A2 - Tissue-specific promoters for gene expression - Google Patents
Tissue-specific promoters for gene expression Download PDFInfo
- Publication number
- WO2000023597A2 WO2000023597A2 PCT/GB1999/003442 GB9903442W WO0023597A2 WO 2000023597 A2 WO2000023597 A2 WO 2000023597A2 GB 9903442 W GB9903442 W GB 9903442W WO 0023597 A2 WO0023597 A2 WO 0023597A2
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- WO
- WIPO (PCT)
- Prior art keywords
- polynucleotide
- expression control
- heterologous
- protein
- plant
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
- C12N15/8223—Vegetative tissue-specific promoters
- C12N15/8229—Meristem-specific, e.g. nodal, apical
Definitions
- This invention relates to the fields of plant biotechnology and plant genetic engineering.
- the invention relates to transgenic plant production and tissue-specific expression of heterologous gene sequences in plants.
- a promoter is a non-coding nucleotide sequence which controls the transcription of an adjacent nucleotide sequence.
- a number of promoters have been isolated from a wide variety of sources, including plants.
- the promoter is only active in particular tissue types expression of the heterologous sequence is restricted accordingly and this may be especially desirable in some circumstances.
- heterologous genes may render the plant resistant to pest or pathogen attack, for example if the heterologous gene encodes a protein toxic to the pest or which inhibits the normal processes of infection by the pathogen.
- Tissue-specific promoters can also be used to activate the expression of heterologous genes encoding proteins which will enhance the nutritional value of the specific tissues where the promoter is active. Such promoters may also be used to modify the level of endogenous gene products in the specific tissues concerned, thereby modulating the biochemistry, physiology or development of the plant.
- tissue-specific promoters which have been well characterised is limited and different promoters exhibit a range of activities which cannot be predicted a priori and are difficult to quantify.
- the activity of a promoter isolated from one species of plant may also differ when it is utilised in a heterologous species - such differences may be both in the tissue specificity and strength of the promoter and are more likely to occur with greater taxonomic distance between plant species.
- different promoters may be required to control expression of multiple genes since gene silencing effects can occur if duplicate copies of the same promoter are used. The choice of promoter is therefore limited and has to be experimentally verified for each system under study.
- an invertase gene expression control polynucleotide a derivative, a functional equivalent, or a part thereof, which is cell-specific to vascular tissue or to nodal tissue .
- cell-specific to vascular tissue we mean that the expression control polynucleotide exhibits a distinct level of activity (or lack of activity) in cells of vascular tissue compared to the other tissue types of the transformed plant.
- cell-specific to nodal tissue we mean that the expression control polyncleotide exhibits a distinct level of activity (or lack of activity) in cells of the node between the stem and the petiole (including the equivalent "eyes" of tubers) compared to other tissue types of the transformed plant .
- control polynucleotide we mean any polynucleotide which is capable of affecting the expression of a gene.
- the term is intended to include promoters, enhancers and suppressors.
- derivative we mean a modified version of the expression control polynucleotide which exhibits substantial sequence homology (for example 70% sequence homology) to the original polynucleotide, for example which include nucleotide substitutions which have no effect on biological function.
- part we mean a deleted version of the expression control polynucleotide, which comprises at least a substantial portion of the original polynucleotide (for example at least 50%, more usually 70%, for example 80% or even 85 to 90%, of said polynucleotide) .
- the preferred type of expression control polynucleotide is a promoter.
- the invertase gene promoter is preferably derived from a dicotyledon, especially a member of the Solanaceae, for example potato.
- the expression control polynucleotide of the invention may comprise double- or single-stranded DNA or RNA.
- the invention also provides the use of the expression control polynucleotide described above to control expression of heterologous sequences.
- the expression control polynucleotide is used to drive cell -specific expression of protein-encoding heterologous genes in plants eg monocotyledons or dicotyledons. Use of the expression control polynucleotide in this way in dicotyledons is preferred.
- the invention also provides a recombinant expression control polynucleotide comprising at least a part of a cell -specific expression control polynucleotide as described above.
- the recombinant expression control polynucleotide of the invention is capable of expression of a heterologous sequence in specific cells.
- the heterologous sequence expressed may encode a protein.
- RNA sequences which do not code for protein eg ribosomal RNA or anti-sense RNA may instead be transcribed from the heterologous sequence.
- the invention also provides a polynucleotide having the sequence set out in SEQ ID Nos 2, 3 and 4, including derivatives, functional equivalents or parts thereof.
- the present invention also provides a recombinant polynucleotide construct comprising an expression control polynucleotide according to the invention.
- the construct may be operably linked to a heterologous (preferably protein-encoding) polynucleotide.
- activation of the expression control polynucleotide may drive the expression of the heterologous polynucleotide, enabling production of the encoded protein. Since the expression control polynucleotide is tissue-specific, production of the protein will be limited to those tissues where the expression control polynucleotide is active.
- the present invention also provides a recombinant vector containing an expression control polynucleotide or a recombinant polynucleotide construct as defined above .
- a method of producing a recombinant vector comprising ligating an expression control polynucleotide as described above into a suitable vector.
- a method of producing a transformed cell by transfecting a host cell using said recombinant vector forms another aspect of the invention. Suitable vectors and genetic modifications thereof are well- known in the art.
- the present invention also provides a transformed host cell containing a recombinant polynucleotide construct or vector as defined above.
- the present invention also provides a transgenic organism (for example a transgenic plant) containing a recombinant polynucleotide construct or a vector as defined above.
- a transgenic organism for example a transgenic plant
- the progeny (and seeds or tubers) of such transgenic organisms forms a further part of the invention.
- the present invention also provides a method for controlling the expression of a protein, said method comprising operably linking a polynucleotide sequence encoding said protein to an expression control polynucleotide of the invention.
- the method is especially useful for the expression of proteins in vascular tissue, for example to suppress or promote budding of potato tubers; to develop "bushier" or non- branching varieties of tomato plants; to express an insecticide (since vascular tissue is a target site for all sap-sucking insects) ; or to promote or reduce growth of the plant (i.e. to obtain taller or dwarf varieties of plants) .
- the invention also provides a method of controlling the expression of a heterologous polynucleotide in vascular tissue, said method comprising operably linking said heterologous polynucleotide to an expression control polynucleotide of the invention.
- the invertase gene promoter described in pGE-GUS is expressed in potatoes to delay or promote stolon formation (i.e. to obtain "early” or “late” varieties of potatoes) .
- the promoter could be expressed in potatoes to delay or promote potato tuber sprouting (i.e. to allow for prolonged storage or to initiate potato plant growth) .
- the present invention encompasses the tubers of such transgenic potato plants.
- the invertase gene promoter described in pCDl-GUS or pCD4-GUS is expressed in a plant (especially a plant of the species of the Solanaceae, for example potatoes or tomatoes) to express an insecticide or insect repelling compound or to express a protein to promote or inhibit plant growth.
- Fig. 1 GUS staining of stems of transgenic potato plants containing the invGE promoter fragment fused to uidA .
- Fig. 2 GUS staining of tuber (under eyes) of transgenic potato plants containing the invGE promoter fragment fused to uidA.
- Fig. 3 GUS staining of stems of transgenic potato plants containing the invCDl promoter fragment fused to uidA in longitudinal (A) and cross (B) section.
- Fig. 4 GUS staining of stems of transgenic potato plants containing the invCD4 promoter fragment fused to uidA in longitudinal (A) and cross (B) section.
- a potato (Solanum tuberosum L . ) cv. Saturna genomic library consisting of a partial Sau3AI digest of genomic DNA cloned into XEMBL3 , was plated to yield 1 x 10 5 pfu which were screened with a radiolabelled carrot invertase cDNA fragment generated by reverse transcription-polymerase chain reaction (RT-PCR) using primers derived from a sequence of carrot cDNA (Sturm and Chrispeels, 1990, The Plant Cell, 2:1107-1119).
- Hybridisation conditions of 5xSSC at 65 °C were utilised with subsequent low stringency washing of filters in 2xSSC at 65 °C. After three rounds of screening two positive clones were obtained plaque pure.
- DNA was purified from one positive clone XGF5 , which was shown to contain an insert of approximately 23 kb of potato DNA.
- This cloned potato DNA was digested with Xfoal and Sail , and fragments cloned into pUC19.
- SEQ ID No 1 A complete DNA sequence of this fragment is presented (SEQ ID No 1) . It was determined, by homology to known invertase gene sequences, that the 5.4 kb of potato DNA carried partial sequences of two invertase genes with the intergenic region constituting the promoter of the downstream gene. This promoter (invGF) forms the basis of a previous patent application (WO 98/41643) .
- the upstream gene (invGE, bp 1-3407) was incomplete in this clone since the 5' end of the clone terminated within intron I and lacked exon I and promoter sequences further upstream.
- invGE The upstream gene
- bp 1-3407 The upstream gene (invGE, bp 1-3407) was incomplete in this clone since the 5' end of the clone terminated within intron I and lacked exon I and promoter sequences further upstream.
- an approach involving nested direct PCR followed by nested inverse PCR was used. Firstly, using potato cv. Saturna DNA as template and primers based on known sequences (Tables 1 and 2), nested PCR generated a DNA fragment of 590 bp which, on cloning and sequencing, was shown to include the remainder of intron I and 50 bp of exon I of invGE .
- a 794 bp fragment from the BarriH.1 site at the 5' end of the promoter sequence to an Alul site 20 bp into the first exon of invGE was isolated, BamHI linkers added, and after digestion with BamHI, the fragment was cloned into pBIlOl to give plasmid pGE-GUS which was used as a vector for stable plant transformation.
- pGE-GUS the promoter fragment is fused to the uidA gene from Escheri chia coli and, when the promoter is active in plants, drives the transcription of this gene to produce the bacterial enzyme /3-glucuronidase (GUS) .
- GUS /3-glucuronidase
- Desiree plants was generated by Agrobacterium tumefaciens- mediated transformation using pGE-GUS as a vector. Plants derived from the use of pGE-GUS as a vector were passed through one cycle of tuberisation then grown in a controlled environment until flowering occurred. A range of tissues including leaf, stem, root, tuber and floral tissues were analysed by a GUS histochemical assay to detect the activity derived form the uidA gene activated by the invertase promoter.
- PCR bp 9-916 of this sequence constituting the invCDlll promoter sequence used further and designated CD1 was amplified using primers containing BarriRI and HindiII sites. After digestion with _3a_nHI and Hindi11 the purified PCR product was cloned into similarly-digested pBIlOl to generate plasmid pCDl-GUS.
- PCR bp 28-1421 of this sequence constituting the invCD141 promoter sequence used further and designated CD4 , was amplified using primers containing Ba ⁇ iH.1 and Hindi11 sited. After digestion with BamHI and HindiI I the purified PCR product was cloned into similarly-digested pBIlOl to generate plasmid pCD4-GUS.
- Plasmids pCDl-GUS and pCD4-GUS were used as vectors for stable plant transformation.
- the promoter fragment is fused to the uidA gene from Escheri chia coli and when the promoter is active in plants would drive the transcription of this gene to produce the bacterial enzyme 3-glucuronidase (GUS) .
- GUS 3-glucuronidase
- a series of transgenic lines of potato (cv. Desiree) plants was generated by Agrobacterium tuwefaciens- mediated transformation using pCDl-GUS as a vector and a second series using pCD4-GUS as a vector.
- Transgenic plants derived from their use as a vector were passed through one cycle of tuberisation then grown in a controlled environment.
- a range of tissues including leaf, stem, root and floral tissues were analysed by a GUS histochemical assay to detect the activity derived from the uidA gene activated by the invertase promoters.
- CD1-GUS transgenic plants strong blue staining was observed in the stem of the plant extending into the petioles and base of the major vein of leaves ( Figures 3a and b) . This staining was consistent with staining in both large and small vascular bundles. A similar pattern of staining was observed in CD4-GUS transgenic plants ( Figures 4a and b) but the staining was less intense indicating a possible lower level of activity of this promoter. Detailed histochemical analysis of the staining region from both sets of transgenics confirmed that the staining was localised in the phloem elements of the vascular bundles. The recombinant DNA procedures utilised were as described by Sambrook et al .
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Abstract
Description
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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AU62215/99A AU6221599A (en) | 1998-10-16 | 1999-10-15 | Tissue-specific promoters for gene expression |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
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GB9822490.0 | 1998-10-16 | ||
GBGB9822490.0A GB9822490D0 (en) | 1998-10-16 | 1998-10-16 | Nucleic acid |
GBGB9823481.8A GB9823481D0 (en) | 1998-10-28 | 1998-10-28 | Nucleic acid |
GB9823481.8 | 1998-10-28 |
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WO2000023597A2 true WO2000023597A2 (en) | 2000-04-27 |
WO2000023597A3 WO2000023597A3 (en) | 2000-07-27 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1576175A2 (en) * | 2002-11-22 | 2005-09-21 | Arborgen LLC | Vascular-preferred promoters |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993004177A1 (en) * | 1991-08-27 | 1993-03-04 | Agricultural Genetics Company Limited | Proteins with insecticidal properties against homopteran insects and their use in plant protection |
DE4213444A1 (en) * | 1992-04-18 | 1993-10-28 | Inst Genbiologische Forschung | Prodn. of potato plants with suppressed tuber sprouting - by genetic modification to reduce sucrose concn. |
WO1997044471A2 (en) * | 1996-05-17 | 1997-11-27 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Potato plants with reduced cytosolic starch phosphorylasis and modified germination |
WO1998011240A2 (en) * | 1996-09-09 | 1998-03-19 | B.C. Research Inc. | A process of increasing plant growth and yield and modifiying cellulose production in plants |
WO1998041643A1 (en) * | 1997-03-19 | 1998-09-24 | Scottish Crop Research Institute | Expression control polynucleotides |
WO1999006578A2 (en) * | 1997-07-30 | 1999-02-11 | Zeneca Limited | Genetic method for controlling sprouting |
DE19734218A1 (en) * | 1997-08-07 | 1999-02-11 | Max Planck Gesellschaft | Processes for increasing yield in plants |
-
1999
- 1999-10-15 AU AU62215/99A patent/AU6221599A/en not_active Abandoned
- 1999-10-15 WO PCT/GB1999/003442 patent/WO2000023597A2/en active Application Filing
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1993004177A1 (en) * | 1991-08-27 | 1993-03-04 | Agricultural Genetics Company Limited | Proteins with insecticidal properties against homopteran insects and their use in plant protection |
DE4213444A1 (en) * | 1992-04-18 | 1993-10-28 | Inst Genbiologische Forschung | Prodn. of potato plants with suppressed tuber sprouting - by genetic modification to reduce sucrose concn. |
WO1997044471A2 (en) * | 1996-05-17 | 1997-11-27 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Potato plants with reduced cytosolic starch phosphorylasis and modified germination |
WO1998011240A2 (en) * | 1996-09-09 | 1998-03-19 | B.C. Research Inc. | A process of increasing plant growth and yield and modifiying cellulose production in plants |
WO1998041643A1 (en) * | 1997-03-19 | 1998-09-24 | Scottish Crop Research Institute | Expression control polynucleotides |
WO1999006578A2 (en) * | 1997-07-30 | 1999-02-11 | Zeneca Limited | Genetic method for controlling sprouting |
DE19734218A1 (en) * | 1997-08-07 | 1999-02-11 | Max Planck Gesellschaft | Processes for increasing yield in plants |
Non-Patent Citations (12)
Title |
---|
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US1995 ASTHIR BAVITA ET AL: "Invertase-mediated interconversion of sucrose and hexoses during their translocation in growing pearl millet plant." Database accession no. PREV199598366192 XP002135167 & JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 1995, vol. 4, no. 1, 1995, pages 23-28, * |
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US1996 TYMOWSKA-LALANNE ZUZANNA ET AL: "Expression and cis-acting elements of the At-beta-fruct1 gene from Arabidopsis thaliana encoding a cell wall invertase." Database accession no. PREV199699006068 XP002135166 & PLANT PHYSIOLOGY AND BIOCHEMISTRY (PARIS) 1996, vol. 34, no. 3, 1996, pages 431-442, ISSN: 0981-9428 * |
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US1997 IVANA MACHACKOVA ET AL: "Growth pattern, tuber formation and hormonal balance in in vitro potato plants carrying ipt gene." Database accession no. PREV199799555659 XP002135168 & PLANT GROWTH REGULATION 1997, vol. 21, no. 1, 1997, pages 27-36, ISSN: 0167-6903 * |
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US1997 ZHANG LONG ET AL: "A pea cell-wall invertase gene (PsInv-1) with tissue-specific expression." Database accession no. PREV199799814349 XP002135165 & PLANT PHYSIOLOGY AND BIOCHEMISTRY (PARIS) 1997, vol. 35, no. 10, 1997, pages 751-760, ISSN: 0981-9428 * |
FU HONGYONG ET AL: "High-level tuber expression and sucrose inducibility of a potato Sus4 sucrose synthase gene require 5' and 3' flanking sequences and the leader intron." PLANT CELL 1995, vol. 7, no. 9, 1995, pages 1387-1394, XP002135161 ISSN: 1040-4651 * |
FU, H., ET AL.: "A potato Sus3 sucrose synthase gene contains a context-dependent 3' element and a leader intron with both positive and negative tissue-specific effects" THE PLANT CELL, vol. 7, no. 9, September 1995 (1995-09), pages 1395-1403, XP002135162 * |
HEDLEY PETER E ET AL: "Potato (Solanum tuberosum) invertase-encoding cDNAs and their differential expression." GENE (AMSTERDAM) 1994, vol. 145, no. 2, 1994, pages 211-214, XP002071296 ISSN: 0378-1119 * |
LERCHL ET AL: "IMPAIRED PHOTOASSIMILATE PARTITIONING CAUSED BY PHLOEM-SPECIFIC REMOVAL OF PYROPHOSPHATE CAN BE COMPLEMENTED BY A PHLOEM-SPECIFIC CYTOSOLIC YEAST-DERIVED INVERTASE IN TRANSGENIC PLANTS" PLANT CELL,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 7, March 1995 (1995-03), pages 259-270, XP002086882 ISSN: 1040-4651 * |
MADDISON, A.L., ET AL.: "Expression of tandem invertase genes associated with sexual and vegetative growth cycles in potato" PLANT MOLECULAR BIOLOGY, vol. 41, December 1999 (1999-12), pages 741-751, XP002135164 * |
MEYER R ET AL: "Promoter deletion analysis of potato invertase gene expression" JOURNAL OF EXPERIMENTAL BOTANY,GB,OXFORD UNIVERSITY PRESS, vol. 45, no. SUPPL, 11 April 1994 (1994-04-11), XP002071293 ISSN: 0022-0957 * |
OHTA S ET AL: "HIGH-LEVEL EXPRESSION OF A SWEET POTATO SPORAMIN GENE PROMOTER BETA GLUCURONIDASE GUS FUSION GENE IN THE STEMS OF TRANSGENIC TOBACCO PLANTS IS CONFERRED BY MULTIPLE CELL TYPE-SPECIFIC REGULATORY ELEMENTS" MOLECULAR & GENERAL GENETICS 1991, vol. 225, no. 3, 1991, pages 369-378, XP002135160 ISSN: 0026-8925 * |
OOSUMI, T., ET AL.: "Solanum chacoense transposable element Sch2, sequence." EMBL ACCESSION NO:U91993, 8 April 1997 (1997-04-08), XP002135188 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1576175A2 (en) * | 2002-11-22 | 2005-09-21 | Arborgen LLC | Vascular-preferred promoters |
EP1576175A4 (en) * | 2002-11-22 | 2007-11-14 | Arborgen Llc | Vascular-preferred promoters |
US7999149B2 (en) | 2002-11-22 | 2011-08-16 | Arborgen, Llc | Vascular-preferred promoter sequences and uses thereof |
Also Published As
Publication number | Publication date |
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AU6221599A (en) | 2000-05-08 |
WO2000023597A3 (en) | 2000-07-27 |
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