CA2381995A1 - Method for the production of proteinaceous substances - Google Patents

Method for the production of proteinaceous substances Download PDF

Info

Publication number
CA2381995A1
CA2381995A1 CA002381995A CA2381995A CA2381995A1 CA 2381995 A1 CA2381995 A1 CA 2381995A1 CA 002381995 A CA002381995 A CA 002381995A CA 2381995 A CA2381995 A CA 2381995A CA 2381995 A1 CA2381995 A1 CA 2381995A1
Authority
CA
Canada
Prior art keywords
production
culture medium
proteinaceous substances
plant material
group
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002381995A
Other languages
French (fr)
Other versions
CA2381995C (en
Inventor
Ralf Reski
Gilbert Gorr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eleva GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2381995A1 publication Critical patent/CA2381995A1/en
Application granted granted Critical
Publication of CA2381995C publication Critical patent/CA2381995C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

The invention relates to a new method for production of heterologous proteinaceous substances in plant material.
In the preferred method selected complete moss plants are cultivated and the desired target substances obtained from the culture medium essentially without disturbing the produced tissues and cells. The method allows a cost effective production of all manner of heterologous proteins in their respective active form under standardisable conditions.

Claims (6)

1. A method for the production of heterologous proteinaceous substances in plant material, characterized in that moss tissue is used as plant material and that the proteinaceous substances produced are obtained from the culture medium essentially without disrupting the producing tissues or cells.
2. The method according to claim 1, characterized in that the proteinaceous substance released into the culture medium is biologically active.
3. The method according to claim 1 or 2, characterized in that a culture medium is used which is essentially free from sugars, vitamins and phytohormones or functional fragments thereof.
4. The method according to any of claims 1 to 3, characterized in that the moss tissue is selected from the group of the mosses including liverworts.
5. The method according to claim 4, characterized in that the moss tissue is selected from mosses of the group consisting of Physcomitrella, Funaria, Sphagnum and Ceratodon.
6. The method according to claim 4, characterized in that the moss tissue is selected from liverworts of the group consisting of Marchantia and Sphaerocarpos.
CA002381995A 1999-10-01 2000-09-27 Method for the production of proteinaceous substances Expired - Lifetime CA2381995C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19947290.4 1999-10-01
DE19947290A DE19947290A1 (en) 1999-10-01 1999-10-01 Process for the production of proteinaceous substances
PCT/DE2000/003374 WO2001025456A2 (en) 1999-10-01 2000-09-27 Method for production of proteinaceous substances

Publications (2)

Publication Number Publication Date
CA2381995A1 true CA2381995A1 (en) 2001-04-12
CA2381995C CA2381995C (en) 2010-01-05

Family

ID=7924139

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002381995A Expired - Lifetime CA2381995C (en) 1999-10-01 2000-09-27 Method for the production of proteinaceous substances

Country Status (31)

Country Link
EP (1) EP1206561B1 (en)
JP (1) JP2003511035A (en)
KR (1) KR100671217B1 (en)
CN (1) CN1192111C (en)
AT (1) ATE232904T1 (en)
AU (1) AU781920B2 (en)
BG (1) BG65487B1 (en)
BR (1) BR0014671A (en)
CA (1) CA2381995C (en)
CZ (1) CZ293818B6 (en)
DE (2) DE19947290A1 (en)
DK (1) DK1206561T3 (en)
EE (1) EE05093B1 (en)
ES (1) ES2192545T3 (en)
HK (1) HK1054053B (en)
HR (1) HRP20020250B1 (en)
HU (1) HU228206B1 (en)
IL (2) IL148638A0 (en)
IS (1) IS6299A (en)
MX (1) MXPA02003359A (en)
NO (1) NO329774B1 (en)
NZ (1) NZ517996A (en)
PL (1) PL200252B1 (en)
PT (1) PT1206561E (en)
RS (1) RS50075B (en)
RU (1) RU2250264C2 (en)
SK (1) SK287740B6 (en)
TR (1) TR200200700T2 (en)
UA (1) UA72552C2 (en)
WO (1) WO2001025456A2 (en)
ZA (1) ZA200202007B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7538259B2 (en) 2003-08-11 2009-05-26 Greenovation Biotech Gmbh Moss expressing promoting regions

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1398383A1 (en) * 2002-09-12 2004-03-17 greenovation Biotech GmbH Protein production method
DE60333201D1 (en) * 2002-09-12 2010-08-12 Greenovation Biotech Gmbh PROCESS FOR THE PRODUCTION OF PROTEINS
DE60213626T2 (en) * 2002-12-20 2007-08-09 Greenovation Biotech Gmbh Process for the preparation of glycosylated proteins in bryophyte cells
US7781197B2 (en) 2002-12-20 2010-08-24 Greenovation Biotech Gmbh Transformed bryophyte cell having disrupted endogenous alpha 1,3-fucosyl and beta 1,2-xylosyl transferase encoding nucleotide sequences for the production of heterologous glycosylated proteins
EP1502954A1 (en) 2003-07-31 2005-02-02 greenovation Biotech GmbH Utilisation of constructs comprising recombination sequence motifes for enhancing gene expression in moss
DE602006015195D1 (en) 2005-07-12 2010-08-12 Greenovation Biotech Gmbh IMPROVEMENTS OF PROTEIN PRODUCTION OR IN CONNECTION WITH THIS
EP1905825A1 (en) 2006-09-29 2008-04-02 greenovation Biotech GmbH Galactosyltransferase
KR200451866Y1 (en) * 2008-07-28 2011-01-14 구동석 Safekeeping cabinet for cellular phone and valuables
JP5641232B2 (en) * 2010-11-24 2014-12-17 石川県公立大学法人 Ogonori-derived cyclooxygenase gene and method for producing prostaglandins using the gene
EP2653548A1 (en) 2012-04-17 2013-10-23 Greenovation Biotech GmbH Method for increasing the secretion of recombinant proteins
RU2663131C1 (en) * 2017-09-06 2018-08-01 федеральное государственное автономное образовательное учреждение высшего образования "Казанский (Приволжский) федеральный университет" (ФГАОУ ВО КФУ) Media for cultivation of human and animal cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8901932A (en) * 1989-07-26 1991-02-18 Mogen Int PRODUCTION OF heterologous PROTEINS IN PLANTS OR PLANTS.
AU6504796A (en) * 1995-07-20 1997-02-18 Washington State University Research Foundation Production of secreted foreign polypeptides in plant cell culture
DE19629402C2 (en) * 1996-07-20 1998-05-14 Dirk Dr Voeste Use of transformed vascular aquatic plants for the production of species-specific or alien substances
WO1998021348A1 (en) * 1996-11-12 1998-05-22 Battelle Memorial Institute Method of producing human growth factors from whole plants or plant cell cultures
JP2001512318A (en) * 1997-02-13 2001-08-21 アプライド フィトロジックス，インコーポレイテッド Production of mature proteins in plants
US6096546A (en) * 1998-01-30 2000-08-01 Board Of Trustees, Rutgers, The State University Of New Jersey Methods for recovering polypeptides from plants and portions thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7538259B2 (en) 2003-08-11 2009-05-26 Greenovation Biotech Gmbh Moss expressing promoting regions
US7834167B2 (en) 2003-08-11 2010-11-16 Greenovation Biotech Gmbh Moss expressing promotion regions

Also Published As

Publication number Publication date
CN1402791A (en) 2003-03-12
PT1206561E (en) 2003-06-30
EP1206561B1 (en) 2003-02-19
NZ517996A (en) 2003-06-30
NO329774B1 (en) 2010-12-13
CN1192111C (en) 2005-03-09
KR20020065478A (en) 2002-08-13
KR100671217B1 (en) 2007-01-18
NO20021201D0 (en) 2002-03-12
JP2003511035A (en) 2003-03-25
SK4152002A3 (en) 2003-01-09
ZA200202007B (en) 2003-09-23
TR200200700T2 (en) 2003-01-21
IL148638A0 (en) 2002-09-12
ES2192545T3 (en) 2003-10-16
ATE232904T1 (en) 2003-03-15
HK1054053A1 (en) 2003-11-14
HU228206B1 (en) 2013-01-28
EE200200159A (en) 2003-04-15
EE05093B1 (en) 2008-10-15
NO20021201L (en) 2002-05-08
RU2250264C2 (en) 2005-04-20
RU2002111666A (en) 2004-03-10
YU24602A (en) 2005-07-19
IL148638A (en) 2008-12-29
DE50001299D1 (en) 2003-03-27
PL354716A1 (en) 2004-02-09
AU1129501A (en) 2001-05-10
WO2001025456A2 (en) 2001-04-12
SK287740B6 (en) 2011-08-04
BR0014671A (en) 2002-07-23
HK1054053B (en) 2005-10-28
MXPA02003359A (en) 2002-10-04
CZ293818B6 (en) 2004-08-18
BG65487B1 (en) 2008-09-30
RS50075B (en) 2009-01-22
EP1206561A2 (en) 2002-05-22
UA72552C2 (en) 2005-03-15
HRP20020250A2 (en) 2004-02-29
AU781920B2 (en) 2005-06-23
BG106547A (en) 2003-04-30
HUP0202628A3 (en) 2004-10-28
CZ20021061A3 (en) 2002-07-17
HUP0202628A2 (en) 2002-12-28
IS6299A (en) 2002-03-13
WO2001025456A3 (en) 2001-12-27
DK1206561T3 (en) 2003-05-26
CA2381995C (en) 2010-01-05
PL200252B1 (en) 2008-12-31
DE19947290A1 (en) 2001-04-19
HRP20020250B1 (en) 2004-12-31

Similar Documents

Publication Publication Date Title
ES2110417T3 (en) METHODS AND COMPOSITIONS FOR THE PRODUCTION OF FERTILE CORN PLANTS, STABLELY TRANSFORMED AND THEIR CELLS.
KR890014734A (en) Manufacturing method of transgenic plant
CA2381995A1 (en) Method for the production of proteinaceous substances
CA2319714A1 (en) Plant having altered environmental stress tolerance
EP2283721A3 (en) Genetically engineered duckweed
JP2003503076A (en) Method for producing substrate for plant growth and block for plant growth
EP0328424A3 (en) Process for the production of somatic embryos
WO1998037173A3 (en) Sugar cane production
MD737F1 (en) Virusfree potato microclonal propagation method
CA2064761A1 (en) Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof
JP2684402B2 (en) Artificial seed germination accelerator
CN1264536A (en) Regeneration promoter for plant
DE68918269D1 (en) PLANT VIRUS CONTROLLERS AND METHOD FOR THE PRODUCTION THEREOF.
WO2002088310A3 (en) Cobra gene and uses thereof
WO2001000785A3 (en) Regeneration of cotton plants
Jager, AK and van Staden Cultured cycads
WO2001042448A3 (en) Artificial promoters for protein expression in plants
JPS61119116A (en) Culture and artificial culture of mushroom (matsutake) by gene synthesis
CA2429724A1 (en) Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
JPWO2008015790A1 (en) Artificial seed and method for promoting germination thereof

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20200928