WO2001000785A3 - Regeneration of cotton plants - Google Patents

Regeneration of cotton plants Download PDF

Info

Publication number
WO2001000785A3
WO2001000785A3 PCT/GB2000/002288 GB0002288W WO0100785A3 WO 2001000785 A3 WO2001000785 A3 WO 2001000785A3 GB 0002288 W GB0002288 W GB 0002288W WO 0100785 A3 WO0100785 A3 WO 0100785A3
Authority
WO
WIPO (PCT)
Prior art keywords
protoplast
callus
cotton plant
regeneration
plant
Prior art date
Application number
PCT/GB2000/002288
Other languages
French (fr)
Other versions
WO2001000785A2 (en
Inventor
Robert David Hall
Dijk Magdalana Maria Gerar Van
Robert Edouard Sevenier
Original Assignee
Zeneca Ltd
Robert David Hall
Dijk Magdalana Maria Gerarda V
Robert Edouard Sevenier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd, Robert David Hall, Dijk Magdalana Maria Gerarda V, Robert Edouard Sevenier filed Critical Zeneca Ltd
Priority to AU56892/00A priority Critical patent/AU5689200A/en
Publication of WO2001000785A2 publication Critical patent/WO2001000785A2/en
Publication of WO2001000785A3 publication Critical patent/WO2001000785A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques

Abstract

The present invention provides methods for the regeneration of a cotton plant comprising culturing a protoplast prepared from somatic cotton plant tissue in a culture medium, wherein the protoplast is embedded in a solid medium; and regenerating a plant from the cultured protoplast. The invention also provides method for the genetic transformation of a cotton plant comprising culturing a protoplast prepared from somatic cotton plant tissue in a culture medium to form callus, wherein the protoplast is embedded in a solid medium; introducing foreign DNA into the callus to form transformed callus; and regenerating a plant from the transformed callus. The invention further provides plants produced according to the methods of the invention, and seeds and progeny of such plants.
PCT/GB2000/002288 1999-06-28 2000-06-13 Regeneration of cotton plants WO2001000785A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU56892/00A AU5689200A (en) 1999-06-28 2000-06-13 Regeneration of cotton plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9915071.6 1999-06-28
GBGB9915071.6A GB9915071D0 (en) 1999-06-28 1999-06-28 Regeneration of cotton plants

Publications (2)

Publication Number Publication Date
WO2001000785A2 WO2001000785A2 (en) 2001-01-04
WO2001000785A3 true WO2001000785A3 (en) 2001-11-29

Family

ID=10856187

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2000/002288 WO2001000785A2 (en) 1999-06-28 2000-06-13 Regeneration of cotton plants

Country Status (4)

Country Link
AR (1) AR024496A1 (en)
AU (1) AU5689200A (en)
GB (1) GB9915071D0 (en)
WO (1) WO2001000785A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102972297B (en) * 2012-12-05 2014-05-07 中国农业科学院生物技术研究所 Method for cultivating regeneration plants of cotton

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
ZA974176B (en) 1996-05-16 1998-09-01 Univ North Texas A rapid in-vitro regeneration scheme of cotton (gossypium hirsutum l.) plants compatible with agrobacterium-mediated transformation
CO4810305A1 (en) 1996-10-10 1999-06-30 Southplains Biotechnologies Inc TRANSFORMATION AND REGENERATION OF FERTILE COTTON PLANTS

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ACTA GENETICA SINICA, vol. 20, no. 6, 1993, pages 536 - 540, ISSN: 0379-4172 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1986, FIROOZABADY E: "THE EFFECTS OF CELL CYCLE PARAMETERS ON THE CELL WALL REGENERATION AND CELL DIVISION OF COTTON PROTOPLASTS GOSSYPIUM-HIRSUTUM", XP002149169, Database accession no. PREV198682106278 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1993, SHE JIANMING ET AL: "High frequency of plating efficiency and plant regeneration from protoplasts of cotton (Gossypium hirsutum L.).", XP002149170, Database accession no. PREV199497275317 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1994, KRENS F A ET AL: "The effect of n-propyl gallate on the formation of ethylene during protoplast isolation in sugarbeet (Beta vulgaris L.).", XP002149171, Database accession no. PREV199598176894 *
DATABASE CAB CAB INTERNATIONAL, WALLINGFORD, OXON, GB; 1987, SAKA, K. ET AL.: "Cell regeneration and sustained division of protoplasts from cotton (Gossypium hirsutum L.)", XP002149168 *
FIROOZABADY: "Isolation culture and cell division in cotyledon protoplasts of cotton Gossypium-hirsutum and Gossypium-barbadense", PLANT CELL REPORTS, vol. 5, 1986, pages 127 - 131, XP000951507 *
JOURNAL OF EXPERIMENTAL BOTANY, vol. 37, no. 181, 1986, pages 1211 - 1217, ISSN: 0022-0957 *
JOURNAL OF EXPERIMENTAL BOTANY, vol. 45, no. 281, 1994, pages 1899 - 1901, ISSN: 0022-0957 *
KHASANOV: "Cultivation of isolated protoplasts from cotyledons of cotton gossypum-hirsutum", SOVIET PLANT PHYSIOLOGY, vol. 26, 1979, pages 77 - 82, XP000951501 *

Also Published As

Publication number Publication date
AR024496A1 (en) 2002-10-02
GB9915071D0 (en) 1999-08-25
AU5689200A (en) 2001-01-31
WO2001000785A2 (en) 2001-01-04

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