WO2000037663A3 - Plant transformation process - Google Patents

Plant transformation process Download PDF

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Publication number
WO2000037663A3
WO2000037663A3 PCT/US1999/030972 US9930972W WO0037663A3 WO 2000037663 A3 WO2000037663 A3 WO 2000037663A3 US 9930972 W US9930972 W US 9930972W WO 0037663 A3 WO0037663 A3 WO 0037663A3
Authority
WO
WIPO (PCT)
Prior art keywords
agrobacterium
seedlings
transgene
plant transformation
transformation process
Prior art date
Application number
PCT/US1999/030972
Other languages
French (fr)
Other versions
WO2000037663A2 (en
Inventor
Maria J Harrison
Anthony T Trieu
Original Assignee
Samuel Roberts Noble Found Inc
Maria J Harrison
Anthony T Trieu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Samuel Roberts Noble Found Inc, Maria J Harrison, Anthony T Trieu filed Critical Samuel Roberts Noble Found Inc
Priority to AU25943/00A priority Critical patent/AU2594300A/en
Priority to EP99968550A priority patent/EP1141356A2/en
Priority to CA002352488A priority patent/CA2352488A1/en
Priority to JP2000589717A priority patent/JP2002533090A/en
Publication of WO2000037663A2 publication Critical patent/WO2000037663A2/en
Publication of WO2000037663A3 publication Critical patent/WO2000037663A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method of Agrobacterium-mediated genetic transformation using seedlings has been found which is applicable to dicots and monocots capable of being transformed by Agrobacterium. The transformation method utilizes vacuum-infiltration to introduce the Agrobacterium T-DNA carrying a gene of interest into the seedlings. Upon maturity, seeds collected from the infiltrated seedlings are germinated, and progeny carrying the transgene are selected. This transformation method produces progeny exhibiting stable inheritance of the transgene without the need for regeneration methods such as somatic embryogenesis or organogenesis.
PCT/US1999/030972 1998-12-23 1999-12-23 Plant transformation process WO2000037663A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU25943/00A AU2594300A (en) 1998-12-23 1999-12-23 Plant transformation process
EP99968550A EP1141356A2 (en) 1998-12-23 1999-12-23 Plant transformation process
CA002352488A CA2352488A1 (en) 1998-12-23 1999-12-23 Plant transformation process
JP2000589717A JP2002533090A (en) 1998-12-23 1999-12-23 Plant transformation method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11371798P 1998-12-23 1998-12-23
US60/113,717 1998-12-23
US14537399P 1999-07-23 1999-07-23
US60/145,373 1999-07-23

Publications (2)

Publication Number Publication Date
WO2000037663A2 WO2000037663A2 (en) 2000-06-29
WO2000037663A3 true WO2000037663A3 (en) 2000-11-09

Family

ID=26811389

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/030972 WO2000037663A2 (en) 1998-12-23 1999-12-23 Plant transformation process

Country Status (5)

Country Link
EP (1) EP1141356A2 (en)
JP (1) JP2002533090A (en)
AU (1) AU2594300A (en)
CA (1) CA2352488A1 (en)
WO (1) WO2000037663A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9574201B2 (en) 2010-06-09 2017-02-21 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering

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PT1306440E (en) * 2000-08-03 2007-02-28 Japan Tobacco Inc Method of improving gene transfer efficiency into plant cells
CN1273604C (en) * 2000-08-03 2006-09-06 日本烟草产业株式会社 Method of improving gene transfer efficiency into plant cells
CN1149918C (en) * 2001-02-26 2004-05-19 山西省农业生物技术研究中心 Method for transferring agrobacterium mediated plant germination seed gene
KR100859715B1 (en) * 2002-07-16 2008-09-23 독립행정법인농업생물자원연구소 Device for electroporation including the use of depressurization/pressurization
BR112012006415B1 (en) 2009-09-22 2020-04-28 Medicago Inc method for preparing plant-derived vlps
MX2012007681A (en) 2009-12-31 2013-01-29 Pioneer Hi Bred Int Engineering plant resistance to diseases caused by pathogens.
NZ708078A (en) 2010-06-02 2017-01-27 Evolva Nutrition Inc Recombinant production of steviol glycosides
ES2614527T3 (en) 2010-11-04 2017-05-31 Medicago Inc. Plant expression system
CN103328636A (en) * 2010-12-22 2013-09-25 菲利普莫里斯生产公司 Method and system for the vacuum infiltration of plants
TWI620816B (en) 2011-03-23 2018-04-11 苜蓿股份有限公司 Method of recovering plant-derived proteins
CN102212553A (en) * 2011-04-15 2011-10-12 山西省农业科学院生物技术研究中心 Ultrasonic-assisted agrobacterium-mediated plant germination seed gene transformation method
CA3128532A1 (en) 2011-08-08 2013-02-14 Evolva Sa Recombinant production of steviol glycosides
CA3171770A1 (en) 2013-02-06 2014-08-14 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m
US10017804B2 (en) 2013-02-11 2018-07-10 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
WO2015143567A1 (en) 2014-03-27 2015-10-01 Medicago Inc. Modified cpmv enhancer elements
EP3167057B1 (en) 2014-07-11 2020-03-25 Medicago Inc. Modifying protein production in plants
BR112017002783A2 (en) 2014-08-11 2017-12-19 Evolva Sa production of steviol glycosides in recombinant hosts
MY191735A (en) 2014-09-09 2022-07-13 Evolva Sa Production of steviol glycosides in recombinant hosts
EP4148137A1 (en) 2015-01-30 2023-03-15 Evolva SA Production of steviol glycosides in recombinant hosts
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
WO2017000074A1 (en) 2015-07-02 2017-01-05 Medicago Inc. Jasmonic acid pathway activator
WO2017025362A1 (en) 2015-08-07 2017-02-16 Evolva Sa Production of steviol glycosides in recombinant hosts
US10982249B2 (en) 2016-04-13 2021-04-20 Evolva Sa Production of steviol glycosides in recombinant hosts
CN109312378A (en) 2016-05-16 2019-02-05 埃沃尔瓦公司 Steviol glycoside is generated in the recombination host
CN110100006A (en) 2016-11-07 2019-08-06 埃沃尔瓦公司 The production of steviol glycoside in recombinant host
WO2022260849A1 (en) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Methods and systems for producing a protein of interest in a plant
CN114657206B (en) * 2022-03-17 2024-03-22 中国热带农业科学院热带作物品种资源研究所 Genetic transformation method of stylosanthes guianensis

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Publication number Priority date Publication date Assignee Title
WO1989005859A1 (en) * 1987-12-21 1989-06-29 The Upjohn Company Agrobacterium mediated transformation of germinating plant seeds
WO1994002620A2 (en) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
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WO1997048814A2 (en) * 1996-06-21 1997-12-24 Monsanto Company Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
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US5569597A (en) * 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
WO1989005859A1 (en) * 1987-12-21 1989-06-29 The Upjohn Company Agrobacterium mediated transformation of germinating plant seeds
WO1994002620A2 (en) * 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
WO1995028423A1 (en) * 1994-04-13 1995-10-26 The General Hospital Corporation Rps gene family, primers, probes, and detection methods
WO1997048814A2 (en) * 1996-06-21 1997-12-24 Monsanto Company Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
WO1998013503A1 (en) * 1996-09-23 1998-04-02 F.B. Investments Pty. Ltd. A plant and method of modification
WO2000022149A1 (en) * 1998-10-15 2000-04-20 Protein Research Trust Transformation process

Non-Patent Citations (11)

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Title
BECHTOLD NICOLE ET AL: "In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants.", COMPTES RENDUS DE L'ACADEMIE DES SCIENCES SERIE III SCIENCES DE LA VIE 1993, vol. 316, no. 10, 1993, pages 1194 - 1199, XP000907460, ISSN: 0764-4469 *
BIOLOGIA PLANTARUM (PRAGUE) 1997-1998, vol. 40, no. 1, 1997, pages 33 - 41, ISSN: 0006-3134 *
CHEE P P ET AL: "TRANSFORMATION OF SOYBEAN GLYCINE-MAX BY INFECTING GERMINATING SEEDS WITH AGROBACTERIUM-TUMEFACIENS", PLANT PHYSIOLOGY (BETHESDA) 1989, vol. 91, no. 3, 1989, pages 1212 - 1218, XP002140336, ISSN: 0032-0889 *
DANHOF LINDA R ET AL: "Transformation of Arabidopsis by vacuum infiltration: A demonstration.", ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS;SAN ANTONIO, TEXAS, USA; JULY 27-31, 1996, vol. 111, no. 2 SUPPL., 1996, Plant Physiology (Rockville) 1996, pages 164, XP002140332, ISSN: 0032-0889 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1995, BECHTOLD N ET AL: "In planta Agrobacterium-mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration.", XP002140338, Database accession no. PREV199698573015 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1996, TRIEU ANTHONY T ET AL: "Rapid transformation of Medicago truncatula: Regeneration via shoot organogenesis.", XP002140339, Database accession no. PREV199799329039 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, RAKOUSKY S ET AL: "Transient beta-glucuronidase activity after infiltration of Arabidopsis thaliana by Agrobacterium tumefaciens.", XP002140337, Database accession no. PREV199800165996 *
GENE TRANSFER TO PLANTS. 1995 SPRINGER-VERLAG;SPRINGER-VERLAG NEW YORK, INC. HEIDELBERGER PLATZ 3, D-1000 BERLIN, GERMANY; 175 FIFTH AVENUE, NEW YORK, NEW YORK 10010, USA, 1995, pages 19 - 23, ISBN: 3-540-58406-4 *
PLANT CELL REPORTS 1996, vol. 16, no. 1-2, 1996, pages 6 - 11, ISSN: 0721-7714 *
TRIEU ANTHONY T ET AL: "Approaches to improve the efficiency of transformation and regeneration of Medicago truncatula.", ANNUAL MEETING OF THE AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS;CHARLOTTE, NORTH CAROLINA, USA; JULY 29-AUGUST 2, 1995, vol. 108, no. 2 SUPPL., 1995, Plant Physiology (Rockville) 1995, pages 57, XP002140335, ISSN: 0032-0889 *
WENCK ALLAN ET AL: "Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation.", PLANT MOLECULAR BIOLOGY 1997, vol. 34, no. 6, 1997, pages 913 - 922, XP002140334, ISSN: 0167-4412 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9574201B2 (en) 2010-06-09 2017-02-21 Bayer Cropscience Nv Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering

Also Published As

Publication number Publication date
AU2594300A (en) 2000-07-12
WO2000037663A2 (en) 2000-06-29
JP2002533090A (en) 2002-10-08
EP1141356A2 (en) 2001-10-10
CA2352488A1 (en) 2000-06-29

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