WO2001059086A3 - Methods and constructs for agrobacterium-mediated plant transformation - Google Patents

Methods and constructs for agrobacterium-mediated plant transformation Download PDF

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Publication number
WO2001059086A3
WO2001059086A3 PCT/CA2001/000136 CA0100136W WO0159086A3 WO 2001059086 A3 WO2001059086 A3 WO 2001059086A3 CA 0100136 W CA0100136 W CA 0100136W WO 0159086 A3 WO0159086 A3 WO 0159086A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
constructs
agrobacterium
plant transformation
novel
Prior art date
Application number
PCT/CA2001/000136
Other languages
French (fr)
Other versions
WO2001059086A2 (en
Inventor
Steven F Fabijanski
Laurian Robert
Johann Schernthaner
Tong Wu
Original Assignee
Sakata Seed Corp
Steven F Fabijanski
Laurian Robert
Johann Schernthaner
Tong Wu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sakata Seed Corp, Steven F Fabijanski, Laurian Robert, Johann Schernthaner, Tong Wu filed Critical Sakata Seed Corp
Priority to JP2001558226A priority Critical patent/JP2003521940A/en
Priority to CA2399469A priority patent/CA2399469C/en
Priority to EP01903550A priority patent/EP1261725A2/en
Priority to AU31462/01A priority patent/AU784571B2/en
Priority to US10/182,616 priority patent/US7112721B2/en
Publication of WO2001059086A2 publication Critical patent/WO2001059086A2/en
Publication of WO2001059086A3 publication Critical patent/WO2001059086A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/743—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Agrobacterium; Rhizobium; Bradyrhizobium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Abstract

The present invention relates to novel methods for the transformation of crop species, novel methods for the selection and identification of transformed plant cells and novel methods for recovery of regenerated whole plants. The method also relates to the development of plants with novel traits and plants that contain novel recombinant DNA constructs.
PCT/CA2001/000136 2000-02-08 2001-02-07 Methods and constructs for agrobacterium-mediated plant transformation WO2001059086A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2001558226A JP2003521940A (en) 2000-02-08 2001-02-07 Novel methods and constructs for plant transformation
CA2399469A CA2399469C (en) 2000-02-08 2001-02-07 Novel methods and constructs for plant transformation
EP01903550A EP1261725A2 (en) 2000-02-08 2001-02-07 Methods and constructs for agrobacterium-mediated plant transformation
AU31462/01A AU784571B2 (en) 2000-02-08 2001-02-07 Novel methods and constructs for plant transformation
US10/182,616 US7112721B2 (en) 2000-02-08 2001-02-07 Methods and constructs for plant transformation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18106300P 2000-02-08 2000-02-08
US60/181,063 2000-02-08

Publications (2)

Publication Number Publication Date
WO2001059086A2 WO2001059086A2 (en) 2001-08-16
WO2001059086A3 true WO2001059086A3 (en) 2001-12-13

Family

ID=22662749

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/000136 WO2001059086A2 (en) 2000-02-08 2001-02-07 Methods and constructs for agrobacterium-mediated plant transformation

Country Status (5)

Country Link
EP (1) EP1261725A2 (en)
JP (1) JP2003521940A (en)
AU (1) AU784571B2 (en)
CA (1) CA2399469C (en)
WO (1) WO2001059086A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1264891A1 (en) * 2001-05-31 2002-12-11 Plant Research International B.V. Modification of plant genomes by inducible site-specific recombination of transgenes
JP2004275011A (en) * 2003-03-12 2004-10-07 National Institute Of Agrobiological Sciences Technique for excising marker gene by transient expression of site-specific recombinant enzyme gene
WO2005121346A1 (en) * 2004-06-08 2005-12-22 New Zealand Institute For Crop & Food Research Transformation vectors
US8791329B2 (en) 2005-02-28 2014-07-29 22Nd Century Limited Llc Reducing levels of nicotinic alkaloids in plants
US8410341B2 (en) 2006-06-19 2013-04-02 National Research Council Of Canada Nucleic acid encoding N-methylputrescine oxidase and uses thereof
US9551003B2 (en) 2006-09-13 2017-01-24 22Nd Century Limited, Llc Increasing levels of nicotinic alkaloids in plants
PT2450446E (en) 2006-09-13 2015-09-15 22Nd Century Ltd Llc Increasing levels of nicotinic alkaloids
US9102948B2 (en) 2006-11-17 2015-08-11 22Nd Century Limited, Llc Regulating alkaloids
CN101578370B (en) 2006-12-20 2015-11-25 孟山都巴西有限公司 The nucleic acid molecule of coding C3HC4 family plant protein and the method for change plant cellulose and lignin content
EP2094852B1 (en) 2006-12-20 2014-01-22 Monsanto do Brasil LTDA Nucleic acid constructs and methods for altering plant fiber length and/or plant height
CN110872589A (en) 2007-05-25 2020-03-10 22世纪有限责任公司 Nucleic acid sequences encoding transcription factors regulating alkaloid synthesis and their use for improving plant metabolism
WO2009136293A2 (en) 2008-05-08 2009-11-12 Alellyx Applied Genomics Genes and methods for increasing disease resistance in plants
MY164892A (en) * 2009-12-04 2018-01-30 Temasek Life Sciences Laboratory Ltd Improved media compositions,selection methods and agrobacterium strains for transformation of plants
US9253952B2 (en) 2012-08-17 2016-02-09 University Of Copenhagen Agrobacterium rhizogenes transformation and expression of rol genes in Kalanchoë
JP7220145B2 (en) 2016-09-20 2023-02-09 トゥエンティセカンド センチュリー リミテッド、 エルエルシー A trichome-specific promoter for the manipulation of cannabinoids and other compounds in the glandular trichome
CN112867794A (en) * 2018-10-04 2021-05-28 株式会社钟化 DNA constructs for genome editing in plants
CN111199773B (en) * 2020-01-20 2023-03-28 中国农业科学院北京畜牧兽医研究所 Evaluation method for fine positioning character associated genome homozygous fragments

Citations (3)

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EP0716147A2 (en) * 1994-11-09 1996-06-12 Nippon Paper Industries Co., Ltd. Methods for producing transgenic plants
US5635381A (en) * 1992-02-26 1997-06-03 Mogen International Nv Agrobacterium bacteria capable of site-specific recombination
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells

Family Cites Families (2)

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CA1340766C (en) * 1984-12-24 1999-09-28 Clive Waldron Selectable marker for development of vectors and transformation systems in plants
EP0298918B1 (en) * 1987-07-10 2001-09-05 Syngenta Participations AG Inducible virus resistance in plants

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5635381A (en) * 1992-02-26 1997-06-03 Mogen International Nv Agrobacterium bacteria capable of site-specific recombination
EP0716147A2 (en) * 1994-11-09 1996-06-12 Nippon Paper Industries Co., Ltd. Methods for producing transgenic plants

Non-Patent Citations (6)

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Title
DALE E C ET AL: "GENE TRANSFER WITH SUBSEQUENT REMOVAL OF THE SELECTION GENE FROM THE HOST GENOME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 88, 1 December 1991 (1991-12-01), pages 10558 - 10562, XP002043803, ISSN: 0027-8424 *
EBINUMA H ET AL: "Selection of marker-free transgenic plants using the isopentenyl transferase gene", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,NATIONAL ACADEMY OF SCIENCE. WASHINGTON,US, vol. 94, March 1997 (1997-03-01), pages 2117 - 2121, XP002102275, ISSN: 0027-8424 *
GLEAVE A P ET AL: "SELECTABLE MARKER-FREE TRANSGENIC PLANTS WITHOUT SEXUAL CROSSING: TRANSIENT EXPRESSION OF CRE RECOMBINASE AND USE OF A CONDITIONAL LETHAL DOMINANT GENE", PLANT MOLECULAR BIOLOGY,NIJHOFF PUBLISHERS, DORDRECHT,NL, vol. 40, May 1999 (1999-05-01), pages 223 - 225, XP000995792, ISSN: 0167-4412 *
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SUGITA KOICHI ET AL: "A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency.", PLANT JOURNAL, vol. 22, no. 5, June 2000 (2000-06-01), pages 461 - 469, XP002173578, ISSN: 0960-7412 *
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Also Published As

Publication number Publication date
EP1261725A2 (en) 2002-12-04
WO2001059086A2 (en) 2001-08-16
AU784571B2 (en) 2006-05-04
CA2399469A1 (en) 2001-08-16
JP2003521940A (en) 2003-07-22
AU3146201A (en) 2001-08-20
CA2399469C (en) 2010-12-14

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