WO2001059086A3 - Methods and constructs for agrobacterium-mediated plant transformation - Google Patents

Methods and constructs for agrobacterium-mediated plant transformation Download PDF

Info

Publication number
WO2001059086A3
WO2001059086A3 PCT/CA2001/000136 CA0100136W WO0159086A3 WO 2001059086 A3 WO2001059086 A3 WO 2001059086A3 CA 0100136 W CA0100136 W CA 0100136W WO 0159086 A3 WO0159086 A3 WO 0159086A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
constructs
agrobacterium
plant transformation
novel
Prior art date
Application number
PCT/CA2001/000136
Other languages
French (fr)
Other versions
WO2001059086A2 (en
Inventor
Steven F Fabijanski
Laurian Robert
Johann Schernthaner
Tong Wu
Original Assignee
Sakata Seed Corp
Steven F Fabijanski
Laurian Robert
Johann Schernthaner
Tong Wu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sakata Seed Corp, Steven F Fabijanski, Laurian Robert, Johann Schernthaner, Tong Wu filed Critical Sakata Seed Corp
Priority to AU31462/01A priority Critical patent/AU784571B2/en
Priority to EP01903550A priority patent/EP1261725A2/en
Priority to US10/182,616 priority patent/US7112721B2/en
Priority to CA2399469A priority patent/CA2399469C/en
Priority to JP2001558226A priority patent/JP2003521940A/en
Publication of WO2001059086A2 publication Critical patent/WO2001059086A2/en
Publication of WO2001059086A3 publication Critical patent/WO2001059086A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/743—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Agrobacterium; Rhizobium; Bradyrhizobium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to novel methods for the transformation of crop species, novel methods for the selection and identification of transformed plant cells and novel methods for recovery of regenerated whole plants. The method also relates to the development of plants with novel traits and plants that contain novel recombinant DNA constructs.
PCT/CA2001/000136 2000-02-08 2001-02-07 Methods and constructs for agrobacterium-mediated plant transformation WO2001059086A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU31462/01A AU784571B2 (en) 2000-02-08 2001-02-07 Novel methods and constructs for plant transformation
EP01903550A EP1261725A2 (en) 2000-02-08 2001-02-07 Methods and constructs for agrobacterium-mediated plant transformation
US10/182,616 US7112721B2 (en) 2000-02-08 2001-02-07 Methods and constructs for plant transformation
CA2399469A CA2399469C (en) 2000-02-08 2001-02-07 Novel methods and constructs for plant transformation
JP2001558226A JP2003521940A (en) 2000-02-08 2001-02-07 Novel methods and constructs for plant transformation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18106300P 2000-02-08 2000-02-08
US60/181,063 2000-02-08

Publications (2)

Publication Number Publication Date
WO2001059086A2 WO2001059086A2 (en) 2001-08-16
WO2001059086A3 true WO2001059086A3 (en) 2001-12-13

Family

ID=22662749

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/000136 WO2001059086A2 (en) 2000-02-08 2001-02-07 Methods and constructs for agrobacterium-mediated plant transformation

Country Status (5)

Country Link
EP (1) EP1261725A2 (en)
JP (1) JP2003521940A (en)
AU (1) AU784571B2 (en)
CA (1) CA2399469C (en)
WO (1) WO2001059086A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1264891A1 (en) * 2001-05-31 2002-12-11 Plant Research International B.V. Modification of plant genomes by inducible site-specific recombination of transgenes
JP2004275011A (en) * 2003-03-12 2004-10-07 National Institute Of Agrobiological Sciences Technique for excising marker gene by transient expression of site-specific recombinant enzyme gene
AU2005252598B8 (en) * 2004-06-08 2011-05-12 The New Zealand Institute For Plant And Food Research Limited Transformation vectors
KR101581039B1 (en) 2005-02-28 2016-01-05 22엔디 센츄리 리미티드, 엘엘씨 Reducing levels of nicotinic alkaloids in plants
WO2008020333A2 (en) 2006-06-19 2008-02-21 National Research Council Of Canada Nucleic acid encoding n-methylputrescine oxidase and uses thereof
PL2792750T3 (en) 2006-09-13 2020-02-28 22Nd Century Limited, Llc Increasing levels of nicotinic alkaloids
US9551003B2 (en) 2006-09-13 2017-01-24 22Nd Century Limited, Llc Increasing levels of nicotinic alkaloids in plants
US9102948B2 (en) 2006-11-17 2015-08-11 22Nd Century Limited, Llc Regulating alkaloids
WO2008074115A1 (en) 2006-12-20 2008-06-26 Alellyx S.A. Nucleic acid constructs and methods for altering plant fiber length and/or plant height
EP2097532B1 (en) 2006-12-20 2014-03-12 Monsanto do Brasil LTDA Nucleic acid molecules encoding plant proteins in the c3hc4 family and methods for the alteration of plant cellulose and lignin content
CA3114078A1 (en) 2007-05-25 2009-05-22 22Nd Century Limited, Llc Nucleic acid sequences encoding transcription factors regulating alkaloid biosynthesis and their use in modifying plant metabolism
EP2682403B1 (en) 2008-05-08 2016-04-20 Monsanto do Brasil LTDA Genes and methods for increasing disease resistance in plants
AU2009356195B2 (en) * 2009-12-04 2015-01-15 Temasek Life Sciences Laboratory Limited Improved media compositions, selection methods and Agrobacterium strains for transformation of plants
US9253952B2 (en) 2012-08-17 2016-02-09 University Of Copenhagen Agrobacterium rhizogenes transformation and expression of rol genes in Kalanchoë
WO2018057385A2 (en) 2016-09-20 2018-03-29 22Nd Century Limited, Llc Trichome specific promoters for the manipulation of cannabinoids and other compounds in glandular trichomes
WO2020071528A1 (en) * 2018-10-04 2020-04-09 株式会社カネカ Dna construct to be used in genome editing of plant
CN111199773B (en) * 2020-01-20 2023-03-28 中国农业科学院北京畜牧兽医研究所 Evaluation method for fine positioning character associated genome homozygous fragments

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0716147A2 (en) * 1994-11-09 1996-06-12 Nippon Paper Industries Co., Ltd. Methods for producing transgenic plants
US5635381A (en) * 1992-02-26 1997-06-03 Mogen International Nv Agrobacterium bacteria capable of site-specific recombination
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340766C (en) * 1984-12-24 1999-09-28 Clive Waldron Selectable marker for development of vectors and transformation systems in plants
ATE205253T1 (en) * 1987-07-10 2001-09-15 Syngenta Participations Ag INDUCABLE VIRUS RESISTANCE IN PLANTS

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5658772A (en) * 1989-12-22 1997-08-19 E. I. Du Pont De Nemours And Company Site-specific recombination of DNA in plant cells
US5635381A (en) * 1992-02-26 1997-06-03 Mogen International Nv Agrobacterium bacteria capable of site-specific recombination
EP0716147A2 (en) * 1994-11-09 1996-06-12 Nippon Paper Industries Co., Ltd. Methods for producing transgenic plants

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DALE E C ET AL: "GENE TRANSFER WITH SUBSEQUENT REMOVAL OF THE SELECTION GENE FROM THE HOST GENOME", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 88, 1 December 1991 (1991-12-01), pages 10558 - 10562, XP002043803, ISSN: 0027-8424 *
EBINUMA H ET AL: "Selection of marker-free transgenic plants using the isopentenyl transferase gene", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,NATIONAL ACADEMY OF SCIENCE. WASHINGTON,US, vol. 94, March 1997 (1997-03-01), pages 2117 - 2121, XP002102275, ISSN: 0027-8424 *
GLEAVE A P ET AL: "SELECTABLE MARKER-FREE TRANSGENIC PLANTS WITHOUT SEXUAL CROSSING: TRANSIENT EXPRESSION OF CRE RECOMBINASE AND USE OF A CONDITIONAL LETHAL DOMINANT GENE", PLANT MOLECULAR BIOLOGY,NIJHOFF PUBLISHERS, DORDRECHT,NL, vol. 40, May 1999 (1999-05-01), pages 223 - 225, XP000995792, ISSN: 0167-4412 *
LLOYD A M ET AL: "FUNCTIONAL EXPRESSION OF THE YEAST FLP/FRT SITE-SPECIFIC RECOMBINATION SYSTEM IN NICOTIANA TABACUM", MOLECULAR AND GENERAL GENETICS,DE,SPRINGER VERLAG, BERLIN, vol. 242, 1994, pages 653 - 657, XP002043804, ISSN: 0026-8925 *
SUGITA KOICHI ET AL: "A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency.", PLANT JOURNAL, vol. 22, no. 5, June 2000 (2000-06-01), pages 461 - 469, XP002173578, ISSN: 0960-7412 *
YODER J I ET AL: "TRANSFORMATION SYSTEMS FOR GENERATING MARKER-FREE TRANSGENIC PLANTS", BIO/TECHNOLOGY,US,NATURE PUBLISHING CO. NEW YORK, vol. 12, no. 3, 1 March 1994 (1994-03-01), pages 263 - 267, XP002043802, ISSN: 0733-222X *

Also Published As

Publication number Publication date
WO2001059086A2 (en) 2001-08-16
CA2399469C (en) 2010-12-14
EP1261725A2 (en) 2002-12-04
CA2399469A1 (en) 2001-08-16
JP2003521940A (en) 2003-07-22
AU784571B2 (en) 2006-05-04
AU3146201A (en) 2001-08-20

Similar Documents

Publication Publication Date Title
WO2001059086A3 (en) Methods and constructs for agrobacterium-mediated plant transformation
ATE365218T1 (en) IMPROVED METHOD FOR AGROBACTERIA-MEDIATED TRANSFORMATION OF COTTON
WO2006056590A3 (en) Plants having increased yield and a method for making the same
WO2005005643A3 (en) Chloroplast transformation of duckweed
WO2007044043A3 (en) Transgenic plants with enhanced agronomic traits
WO2003004659A8 (en) Recombination systems and a method for removing nucleic acid sequences from the genome of eukaryotic organisms
WO2005004585A3 (en) Precise breeding
WO2010125036A3 (en) Plants having enhanced yield-related traits and a method for making the same
HK1084415A1 (en) Hybrid spring oilseed rape and methods for producing same
WO2009099580A3 (en) Transgenic soy plants with enhanced agronomic traits
WO2008142163A3 (en) Plants having enhanced yield-related traits and a method for making the same
WO2003027247A3 (en) Modulation of stem cells using zinc finger proteins
WO2000037663A3 (en) Plant transformation process
ZA200703426B (en) Plants having improved growth characteristics and method for making the same
WO2004087755A3 (en) Plant haemoglobin
WO2003010319A3 (en) Transformation method for obtaining marker-free plants and plants obtained therewith
WO2006058897A3 (en) Plants having improved growth characteristics and method for making the same
WO2000028014A3 (en) Plastid transformation of solanaceous plants
WO2002006500A3 (en) Methods of transforming plants and identifying parental origin of a chromosome in those plants
WO2005085452A8 (en) Plants having improved yield and method for making the same
WO2001094393A3 (en) Synthetic spider silk proteins and the expression thereof in transgenic plants
WO2000075359A3 (en) Gene encoding short integuments and uses thereof
WO2002086112A3 (en) Nucleic acid molecules encoding starch degrading enzymes
WO2001070778A3 (en) Maize glycine rich protein promoter and use thereof
EP1116794A3 (en) Transgenic plants carrying neoxanthin cleavage enzyme gene

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 31462/01

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2001903550

Country of ref document: EP

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 558226

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2399469

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 10182616

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2001903550

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642