WO2000063400A3 - Plant transformation process - Google Patents

Plant transformation process Download PDF

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Publication number
WO2000063400A3
WO2000063400A3 PCT/US2000/010704 US0010704W WO0063400A3 WO 2000063400 A3 WO2000063400 A3 WO 2000063400A3 US 0010704 W US0010704 W US 0010704W WO 0063400 A3 WO0063400 A3 WO 0063400A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
agrobacterium
transgene
transformation
plant transformation
Prior art date
Application number
PCT/US2000/010704
Other languages
French (fr)
Other versions
WO2000063400A2 (en
Inventor
Maria J Harrison
Stephen H Burliegh
Igor Kardailsky
Original Assignee
Samuel Roberts Noble Found Inc
Maria J Harrison
Stephen H Burliegh
Igor Kardailsky
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Samuel Roberts Noble Found Inc, Maria J Harrison, Stephen H Burliegh, Igor Kardailsky filed Critical Samuel Roberts Noble Found Inc
Priority to EP00923550A priority Critical patent/EP1171618A2/en
Priority to CA002370638A priority patent/CA2370638A1/en
Priority to AU43652/00A priority patent/AU4365200A/en
Publication of WO2000063400A2 publication Critical patent/WO2000063400A2/en
Publication of WO2000063400A3 publication Critical patent/WO2000063400A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

A method of Agrobacterium-mediated genetic transformation using plants at the time of flowering has been found which is applicable to dicots and monocots capable of being transformed by Agrobacterium. The transformation method utilizes vacuum-infiltration to introduce the Agrobacterium T-DNA carrying a gene of interest into the plants, preferably plants grown from vernalized seed. Upon maturity, seeds collected from the infiltrated plants are germinated, and progeny carrying the transgene are selected. This transformation method produces progeny exhibiting stable inheritance of the transgene without the need for regeneration methods such as somatic embryogenesis or organogenesis.
PCT/US2000/010704 1999-04-21 2000-04-20 Plant transformation process WO2000063400A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00923550A EP1171618A2 (en) 1999-04-21 2000-04-20 Plant transformation process
CA002370638A CA2370638A1 (en) 1999-04-21 2000-04-20 Plant transformation process
AU43652/00A AU4365200A (en) 1999-04-21 2000-04-20 Plant transformation process

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13053199P 1999-04-21 1999-04-21
US60/130,531 1999-04-21

Publications (2)

Publication Number Publication Date
WO2000063400A2 WO2000063400A2 (en) 2000-10-26
WO2000063400A3 true WO2000063400A3 (en) 2001-01-18

Family

ID=22445129

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/010704 WO2000063400A2 (en) 1999-04-21 2000-04-20 Plant transformation process

Country Status (5)

Country Link
EP (1) EP1171618A2 (en)
AU (1) AU4365200A (en)
CA (1) CA2370638A1 (en)
NZ (1) NZ513993A (en)
WO (1) WO2000063400A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1495670A4 (en) * 2002-04-15 2005-04-13 Phytoculture Control Co Ltd Plant transformation system
ES2299285B2 (en) * 2004-11-26 2009-12-07 Universidad De Vigo PROCEDURE FOR TRANSFORMING VEGETABLE MATERIAL FROM ADULT TREES.
KR20120093223A (en) 2009-09-22 2012-08-22 메디카고 인코포레이티드 Method of preparing plant-derived proteins
CN103179850B (en) 2010-06-02 2016-03-16 沃维公司 The recombinant production of steviol glycoside
US20130295609A1 (en) 2010-11-04 2013-11-07 Medicago Inc. Plant expression system
TWI620816B (en) 2011-03-23 2018-04-11 苜蓿股份有限公司 Method of recovering plant-derived proteins
BR122020019161B1 (en) 2011-08-08 2022-04-05 Evolva Sa recombinant host
MX369525B (en) 2013-02-06 2019-11-11 Evolva Sa Methods for improved production of rebaudioside d and rebaudioside m.
WO2014122328A1 (en) 2013-02-11 2014-08-14 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
AU2015234595B2 (en) 2014-03-27 2021-03-25 Medicago Inc. Modified CPMV enhancer elements
RU2731295C2 (en) 2014-07-11 2020-09-01 Медикаго Инк. Modification of protein production in plants
MX2017001859A (en) 2014-08-11 2017-04-11 Evolva Sa Production of steviol glycosides in recombinant hosts.
SG11201701677UA (en) 2014-09-09 2017-04-27 Evolva Sa Production of steviol glycosides in recombinant hosts
SG11201705606PA (en) 2015-01-30 2017-08-30 Evolva Sa Production of steviol glycosides in recombinant hosts
CN107567492B (en) 2015-03-16 2022-10-14 帝斯曼知识产权资产管理有限公司 UDP-glycosyltransferase
ES2857741T3 (en) 2015-07-02 2021-09-29 Medicago Inc Activator of the jasmonic acid pathway
MX2018001620A (en) 2015-08-07 2018-06-11 Evolva Sa Production of steviol glycosides in recombinant hosts.
MX2018012561A (en) 2016-04-13 2019-01-17 Evolva Sa Production of steviol glycosides in recombinant hosts.
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN110100006A (en) 2016-11-07 2019-08-06 埃沃尔瓦公司 The production of steviol glycoside in recombinant host
EP4351320A1 (en) 2021-06-09 2024-04-17 ImmunityBio, Inc. Methods and systems for producing a protein of interest in a plant

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BECHTOLD N ET AL: "IN PLANTA AGROBACTERIUM MEDIATED GENE TRANSFER BY INFILTRATION OF ADULT ARABIDOPSIS THALIANA PLANTS", COMPTES RENDUS DES SEANCES DE L'ACADEMIE DES SCIENCES. SERIE III: SCIENCES DE LA VIE,NL,ELSEVIER, AMSTERDAM, vol. 316, no. 10, 1993, pages 1194 - 1199, XP000907460, ISSN: 0764-4469 *
BECHTOLD N ET AL: "IN PLANTA AGROBACTERIUM-MEDIATED TRANSFORMATION OF ADULT ARABIDOPSIS THALIANA PLANTS BY VACUUM INFILTRATION", METHODS IN MOLECULAR BIOLOGY,US,HUMANA PRESS INC., CLIFTON, NJ, vol. 82, 1998, pages 259 - 266, XP000914126 *
PLANT CELL REPORTS, vol. 16, no. 1-2, 1996, pages 6 - 11 *
TRIEU ANTHONY T ET AL: "Approaches to improve the efficiency of transformation and regeneration of Medicago truncatula", PLANT PHYSIOLOGY,US,AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, vol. 108, no. 2 SUPPL, 29 July 1995 (1995-07-29), pages 57, XP002140335, ISSN: 0032-0889 *
TRIEU ANTHONY T ET AL: "Rapid transformation of Medicago truncatula: Regeneration via shoot organogenesis", BIOSIS, XP002140339 *

Also Published As

Publication number Publication date
WO2000063400A2 (en) 2000-10-26
CA2370638A1 (en) 2000-10-26
NZ513993A (en) 2001-09-28
EP1171618A2 (en) 2002-01-16
AU4365200A (en) 2000-11-02

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