WO1995005471A3 - Method for stable transformation of plants - Google Patents

Method for stable transformation of plants Download PDF

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Publication number
WO1995005471A3
WO1995005471A3 PCT/EP1994/002566 EP9402566W WO9505471A3 WO 1995005471 A3 WO1995005471 A3 WO 1995005471A3 EP 9402566 W EP9402566 W EP 9402566W WO 9505471 A3 WO9505471 A3 WO 9505471A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
plants
stable transformation
plant material
protein complex
Prior art date
Application number
PCT/EP1994/002566
Other languages
French (fr)
Other versions
WO1995005471A2 (en
Inventor
Barbara Hohn
Fabrice Schoumacher
Erich Lanka
Werner Pansegrau
Peter Rolf Scheiffele
Original Assignee
Ciba Geigy Ag
Max Planck Gesellschaft
Barbara Hohn
Fabrice Schoumacher
Erich Lanka
Werner Pansegrau
Peter Rolf Scheiffele
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy Ag, Max Planck Gesellschaft, Barbara Hohn, Fabrice Schoumacher, Erich Lanka, Werner Pansegrau, Peter Rolf Scheiffele filed Critical Ciba Geigy Ag
Priority to AU75345/94A priority Critical patent/AU7534594A/en
Publication of WO1995005471A2 publication Critical patent/WO1995005471A2/en
Publication of WO1995005471A3 publication Critical patent/WO1995005471A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria

Abstract

The present invention relates to a method for producing stably transformed plant material using a specifically designed DNA/protein complex comprising in operable linkage to an expressible DNA a VirD2 protein. The invention further relates to the said DNA/protein complex itself and to the plant material transformed therewith.
PCT/EP1994/002566 1993-08-13 1994-08-03 Method for stable transformation of plants WO1995005471A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU75345/94A AU7534594A (en) 1993-08-13 1994-08-03 Method for stable transformation of plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93113028 1993-08-13
EP93113028.0 1993-08-13

Publications (2)

Publication Number Publication Date
WO1995005471A2 WO1995005471A2 (en) 1995-02-23
WO1995005471A3 true WO1995005471A3 (en) 1995-06-01

Family

ID=8213176

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1994/002566 WO1995005471A2 (en) 1993-08-13 1994-08-03 Method for stable transformation of plants

Country Status (2)

Country Link
AU (1) AU7534594A (en)
WO (1) WO1995005471A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU227410B1 (en) * 1995-09-25 2011-05-30 Syngenta Participations Ag Improved integration of exogenous dna delivered to plant cells
US6051409A (en) * 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
EP1080197A2 (en) * 1998-05-22 2001-03-07 Pioneer Hi-Bred International, Inc. Cell cycle genes, proteins and uses thereof
GB9816138D0 (en) * 1998-07-24 1998-09-23 Ciba Geigy Ag Organic compounds
CN1253568C (en) * 2002-09-02 2006-04-26 大连科源农业生物工程有限公司 Biosafety operating procedure of plant gene engineering
JP2005080570A (en) * 2003-09-09 2005-03-31 Univ Osaka Method for introducing biological material, and transformation method using the method for introducing the biological material
JP5684473B2 (en) 2006-07-19 2015-03-11 モンサント テクノロジー エルエルシー Use of multiple transformation enhancer sequences to improve plant transformation efficiency

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009776A1 (en) * 1988-04-14 1989-10-19 Regents Of The University Of Minnesota VirE OPERON-ENCODED POLYPEPTIDES THAT BIND SINGLE-STRANDED DNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989009776A1 (en) * 1988-04-14 1989-10-19 Regents Of The University Of Minnesota VirE OPERON-ENCODED POLYPEPTIDES THAT BIND SINGLE-STRANDED DNA

Non-Patent Citations (13)

* Cited by examiner, † Cited by third party
Title
BIOLOGICAL ABSTRACTS, vol. 89, 1990, Philadelphia, PA, US; abstract no. 104025, SRINIVASAN, M.F.Y., ET AL.: "25-bp long border sequence of T-DNA is sufficient for cleavage by vird endonuclease of Agrobacterium" *
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; VOGEL, A.M., ET AL.: "Mutational analysis of Agrobacterium tumefaciens pTiA6 virD1: identification of functionally important residues" *
FILICHKIN, S.A., ET AL.: "Formation of a putative relaxation intermediate during T-DNA processing directed by the Agrobacterium tumefaciens VirD1,D2 endonuclease", MOLECULAR MICROBIOLOGY, vol. 8, no. 5, May 1993 (1993-05-01), pages 915 - 926, XP002982170, DOI: doi:10.1111/j.1365-2958.1993.tb01637.x *
GHAI, J., ET AL.: "The VirD operon of Agrobacterium tumefaciens Ti plasmid encodes a DNA relaxing enzyme", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 86, May 1989 (1989-05-01), WASHINGTON US, pages 3109 - 3113, XP000007956, DOI: doi:10.1073/pnas.86.9.3109 *
HERREA-ESTRELLA, A., ET AL.: "VirD proteins of Agrobacterium tumefaciens are required for the formation of a covalent DNA - protein complex at the 5' terminus of T-strand molecules", EMBO JOURNAL, vol. 7, no. 13, 1988, EYNSHAM, OXFORD GB, pages 4055 - 4062 *
HOWARD, E.A., ET AL.: "Activation of the T-DNA transfer process in Agrobacterium results in the generation of a T-strand-protein complex: Tight association of virD2 with the 5' ends of T-strands", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 86, no. 11, June 1989 (1989-06-01), WASHINGTON US, pages 4017 - 4021, XP000026479, DOI: doi:10.1073/pnas.86.11.4017 *
J. GENET., vol. 68, no. 3, 1989, pages 161 - 170 *
JASPER, F., ET AL.: "Agrobacterium T-strand production in vitro: Sequence-specific cleavage and 5' protection of single-stranded DNA templates by purified VirD2 protein", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 91, January 1994 (1994-01-01), WASHINGTON US, pages 694 - 698 *
MOLECULAR MICROBIOLOGY, vol. 12, no. 5, 1994, pages 811 - 817 *
PANSEGRAU, W., ET AL.: "Relaxase (TraI) of IncPalpha plasmid RP4 catalyzes a site-specific cleaving-joining reaction of single-stranded DNA", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 90, April 1993 (1993-04-01), WASHINGTON US, pages 2925 - 2929 *
PANSEGRAU, W., ET AL.: "Site-specific cleavage and joining of single-stranded DNA by VirD2 protein of Agrobacterium tumefaciens Ti plasmids: Analogy to bacterial conjugation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 90, December 1993 (1993-12-01), WASHINGTON US, pages 11538 - 11642 *
ROSSI, L., ET AL.: "The VirD2 protein of Agrobacterium tumefaciens carries nuclear localization signals important for transfer of T-DNA to plants", MOLECULAR AND GENERAL GENETICS, vol. 239, no. 3, June 1993 (1993-06-01), BERLIN DE, pages 345 - 353, XP008082912, DOI: doi:10.1007/BF00276932 *
VOGEL, A.M., ET AL.: "Mutational analysis of Agrobacterium tunefaciens virD2: tyrosine 29 is essential for endonuclease activity", J. BACTERIOLOGY, vol. 174, no. 1, January 1992 (1992-01-01), pages 303 - 308 *

Also Published As

Publication number Publication date
AU7534594A (en) 1995-03-14
WO1995005471A2 (en) 1995-02-23

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