WO2000022163A3 - Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen - Google Patents

Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen Download PDF

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Publication number
WO2000022163A3
WO2000022163A3 PCT/EP1999/007709 EP9907709W WO0022163A3 WO 2000022163 A3 WO2000022163 A3 WO 2000022163A3 EP 9907709 W EP9907709 W EP 9907709W WO 0022163 A3 WO0022163 A3 WO 0022163A3
Authority
WO
WIPO (PCT)
Prior art keywords
kit
amino acid
antigens
directly detecting
acid sequences
Prior art date
Application number
PCT/EP1999/007709
Other languages
English (en)
French (fr)
Other versions
WO2000022163A2 (de
Inventor
Werner Lehmann
Original Assignee
Attomol Gmbh Molekulare Diagno
Werner Lehmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Attomol Gmbh Molekulare Diagno, Werner Lehmann filed Critical Attomol Gmbh Molekulare Diagno
Priority to AU64713/99A priority Critical patent/AU6471399A/en
Priority to EP99952563A priority patent/EP1121469A2/de
Publication of WO2000022163A2 publication Critical patent/WO2000022163A2/de
Publication of WO2000022163A3 publication Critical patent/WO2000022163A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein als Restrictase Chain Reaction (RCR) bezeichnetes Verfahren zum direkten Nachweis von Target-Nukleotid- oder Target-Aminosäuresequenzen oder von Target-Antigenen durch Amplifikation einer Nukleotidsequenz mittels einer DNS-Polymerase, eines Restriktionsenzyms und einer Matrix-DNS, an der durch Bindung eines initialen Primers dieser Primer amplifiziert wird. Desweiteren betrifft die Erfindung einen diese Matrix-DNS beinhaltenden Kit und die Verwendung dieses Kits in der medizinischen Diagnostik und kriminalistischen Spurenanalytik sowie für Umwelt-, Lebens- und Arzneimittelanalysen.
PCT/EP1999/007709 1998-10-12 1999-10-11 Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen WO2000022163A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU64713/99A AU6471399A (en) 1998-10-12 1999-10-11 Method and kit for directly detecting nucleotide sequences, amino acid sequencesor antigens
EP99952563A EP1121469A2 (de) 1998-10-12 1999-10-11 Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19848297.3 1998-10-12
DE19848297 1998-10-12

Publications (2)

Publication Number Publication Date
WO2000022163A2 WO2000022163A2 (de) 2000-04-20
WO2000022163A3 true WO2000022163A3 (de) 2000-08-03

Family

ID=7885036

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/007709 WO2000022163A2 (de) 1998-10-12 1999-10-11 Verfahren und kit zum direkten nachweis von nukleotidsequenzen, aminosäuresequenzen oder antigenen

Country Status (3)

Country Link
EP (1) EP1121469A2 (de)
AU (1) AU6471399A (de)
WO (1) WO2000022163A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10164219A1 (de) * 2001-12-31 2003-07-17 Reinald Repp Verfahren zur Detektion von Nukleinsäuren unter Verwendung festphasengebundener Primer und Abspaltung der Produkte einer zyklischen Amplifikationsreaktion (Trap Release Primer Amplification (TRAmp)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990006045A2 (en) * 1988-11-21 1990-06-14 Dynal As Nucleic acid probes
EP0408918A1 (de) * 1989-06-23 1991-01-23 Canon Kabushiki Kaisha Verfahren zum Nachweis von Nucleinsäuren
WO1992001813A1 (en) * 1990-07-25 1992-02-06 Syngene, Inc. Circular extension for generating multiple nucleic acid complements
US5405746A (en) * 1988-03-23 1995-04-11 Cemu Bioteknik Ab Method of sequencing DNA

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5405746A (en) * 1988-03-23 1995-04-11 Cemu Bioteknik Ab Method of sequencing DNA
WO1990006045A2 (en) * 1988-11-21 1990-06-14 Dynal As Nucleic acid probes
EP0408918A1 (de) * 1989-06-23 1991-01-23 Canon Kabushiki Kaisha Verfahren zum Nachweis von Nucleinsäuren
WO1992001813A1 (en) * 1990-07-25 1992-02-06 Syngene, Inc. Circular extension for generating multiple nucleic acid complements

Also Published As

Publication number Publication date
AU6471399A (en) 2000-05-01
WO2000022163A2 (de) 2000-04-20
EP1121469A2 (de) 2001-08-08

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