WO1999058699A3 - Facteurs de coagulation du sang humain derives de plantes transgeniques - Google Patents

Facteurs de coagulation du sang humain derives de plantes transgeniques Download PDF

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Publication number
WO1999058699A3
WO1999058699A3 PCT/US1999/010732 US9910732W WO9958699A3 WO 1999058699 A3 WO1999058699 A3 WO 1999058699A3 US 9910732 W US9910732 W US 9910732W WO 9958699 A3 WO9958699 A3 WO 9958699A3
Authority
WO
WIPO (PCT)
Prior art keywords
coagulation factors
plant
transgenic plant
human blood
derived human
Prior art date
Application number
PCT/US1999/010732
Other languages
English (en)
Other versions
WO1999058699A2 (fr
Inventor
Brian S Hooker
Jianwei Gao
Daniel B Anderson
Ziyu Dai
Original Assignee
Battelle Memorial Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Battelle Memorial Institute filed Critical Battelle Memorial Institute
Priority to EP99921969A priority Critical patent/EP1076698A2/fr
Priority to JP2000548490A priority patent/JP2002514433A/ja
Priority to CA002328493A priority patent/CA2328493A1/fr
Publication of WO1999058699A2 publication Critical patent/WO1999058699A2/fr
Publication of WO1999058699A3 publication Critical patent/WO1999058699A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'invention concerne une composition pour plantes transgéniques et pour des facteurs de coagulation humains dérivés de ces plantes transgéniques et capables d'éliciter une réaction d'activation dans le processus de coagulation et par conséquent utiles pour le traitement de patients humains chez lesquels une déficience des protéines du facteur de coagulation a été diagnostiquée. Ces protéines peuvent être produites par des procédés permettant une production non virale, comprenant l'utilisation de plantes entières et de cultures de cellules de plantes. L'invention concerne également des vecteurs d'expression assurant une transformation correcte du tissu végétal pour la production de ces facteurs, ainsi que des cellules de plantes transformées et des procédés permettant de produire des facteurs de coagulation humains par des techniques de biologie moléculaire végétale.
PCT/US1999/010732 1998-05-14 1999-05-14 Facteurs de coagulation du sang humain derives de plantes transgeniques WO1999058699A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99921969A EP1076698A2 (fr) 1998-05-14 1999-05-14 Facteurs de coagulation du sang humain derives de plantes transgeniques
JP2000548490A JP2002514433A (ja) 1998-05-14 1999-05-14 トランスジェニック植物由来ヒト凝血因子
CA002328493A CA2328493A1 (fr) 1998-05-14 1999-05-14 Facteurs de coagulation du sang humain derives de plantes transgeniques

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US8000398A 1998-05-14 1998-05-14
US09/080,003 1998-05-14
US30684799A 1999-05-06 1999-05-06
US09/306,847 1999-05-06

Publications (2)

Publication Number Publication Date
WO1999058699A2 WO1999058699A2 (fr) 1999-11-18
WO1999058699A3 true WO1999058699A3 (fr) 2000-04-06

Family

ID=26762686

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/010732 WO1999058699A2 (fr) 1998-05-14 1999-05-14 Facteurs de coagulation du sang humain derives de plantes transgeniques

Country Status (4)

Country Link
EP (1) EP1076698A2 (fr)
JP (1) JP2002514433A (fr)
CA (1) CA2328493A1 (fr)
WO (1) WO1999058699A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1282439B1 (fr) * 2000-05-10 2005-11-30 Novo Nordisk Health Care AG COMPOSITION PHARMACEUTIQUE COMPRENANT UN FACTEUR VIIa ET UN INHIBITEUR DE TFPI
ES2640613T3 (es) * 2000-07-21 2017-11-03 Revance Therapeutics, Inc. Sistemas de transporte de agentes biológicos de múltiples componentes
ATE423199T1 (de) * 2001-07-06 2009-03-15 Chemo Sero Therapeut Res Inst Verfahren zur herstelllung von menschlichem thrombin mittels genmodifikationstechnik
CZ2004543A3 (cs) * 2001-11-09 2004-08-18 Novoánordiskáa@S Farmaceutický prostředek obsahující polypeptidy faktoru VII a polypeptidy faktoru V
US7125846B2 (en) 2001-11-09 2006-10-24 Novo Nordisk Healthcare A/G Pharmaceutical composition comprising factor VII polypeptides and factor V polypeptides
WO2005123928A1 (fr) * 2004-06-08 2005-12-29 Battelle Memorial Institute Production du facteur viii de coagulation humain à partir de cellules végétales et de plantes entières
WO2006004675A2 (fr) 2004-06-25 2006-01-12 Altor Bioscience Corporation Production d'un facteur tissulaire chez des plantes
CA3217359A1 (fr) * 2022-10-18 2024-04-18 Proview-Mbd Biotech Co., Ltd. Production d'alpha-thrombine humaine recombinante dans un systeme a base de plante et application connexe

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0268772A2 (fr) * 1986-09-19 1988-06-01 ZymoGenetics, Inc. Expression du facteur XIII biologiquement actif
WO1991002066A1 (fr) * 1989-07-26 1991-02-21 Mogen International N.V. Production de proteines heterologues dans les vegetaux et cellules vegetales
WO1993013208A1 (fr) * 1991-12-31 1993-07-08 Zymogenetics, Inc. Procedes de production de thrombine
WO1994017415A1 (fr) * 1993-01-29 1994-08-04 Dahlbaeck Bjoern Nouvelle activite de cofacteur anticoagulant
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0268772A2 (fr) * 1986-09-19 1988-06-01 ZymoGenetics, Inc. Expression du facteur XIII biologiquement actif
WO1991002066A1 (fr) * 1989-07-26 1991-02-21 Mogen International N.V. Production de proteines heterologues dans les vegetaux et cellules vegetales
WO1993013208A1 (fr) * 1991-12-31 1993-07-08 Zymogenetics, Inc. Procedes de production de thrombine
WO1994017415A1 (fr) * 1993-01-29 1994-08-04 Dahlbaeck Bjoern Nouvelle activite de cofacteur anticoagulant
US5723755A (en) * 1995-05-16 1998-03-03 Francis E. Lefaivre Large scale production of human or animal proteins using plant bioreactors

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CRAMER C L ET AL: "Bioproduction of human enzymes in transgenic tobacco.", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, (1996 MAY 25) 792 62-71. REF 50, XP000856387 *
DAI Z Y (REPRINT) ET AL: "Expression of human coagulation factor VIII gene in tobacco.", PLANT PHYSIOLOGY, (JUL 1997) VOL. 114, NO. 3, SUPP. [S], PP. 1584-1584. PUBLISHER: AMER SOC PLANT PHYSIOLOGISTS, 15501 MONONA DRIVE, ROCKVILLE, M 20855. ISSN: 0032-0889., BATTELLE MEM INST, PACIFIC NW LABS, BIOPROC GRP, RICHLAND, WA 99352, XP002123654 *
KANE W H ET AL: "Expression and characterization of recombinant human factor V and a mutant lacking a major portion of the connecting region.", BIOCHEMISTRY, (1990 JUL 24) 29 (29) 6762-8., XP002123653 *

Also Published As

Publication number Publication date
JP2002514433A (ja) 2002-05-21
EP1076698A2 (fr) 2001-02-21
CA2328493A1 (fr) 1999-11-18
WO1999058699A2 (fr) 1999-11-18

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