WO1999058637A3 - Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen - Google Patents

Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen Download PDF

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Publication number
WO1999058637A3
WO1999058637A3 PCT/DE1999/001233 DE9901233W WO9958637A3 WO 1999058637 A3 WO1999058637 A3 WO 1999058637A3 DE 9901233 W DE9901233 W DE 9901233W WO 9958637 A3 WO9958637 A3 WO 9958637A3
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WIPO (PCT)
Prior art keywords
sound waves
sample
biological sample
sample container
targeted exposure
Prior art date
Application number
PCT/DE1999/001233
Other languages
English (en)
French (fr)
Other versions
WO1999058637A2 (de
Inventor
Thomas Hahn
Bernhard Kleffner
Hans Ruf
Original Assignee
Fraunhofer Ges Forschung
Thomas Hahn
Bernhard Kleffner
Hans Ruf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Ges Forschung, Thomas Hahn, Bernhard Kleffner, Hans Ruf filed Critical Fraunhofer Ges Forschung
Priority to US09/674,813 priority Critical patent/US6699711B1/en
Priority to EP99927719A priority patent/EP1075507A2/de
Publication of WO1999058637A2 publication Critical patent/WO1999058637A2/de
Publication of WO1999058637A3 publication Critical patent/WO1999058637A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Beschrieben wird eine Vorrichtung zur gezielten Beaufschlagung einer biologischen Probe, vorzugsweise biologisches Zellmaterial, mit Schallwellen, mit einem Probengefäß, in dem die biologische Probe in suspendierter Form vorliegt, sowie mit einer die Schallwellen erzeugenden elektroakustischen Wandlereinrichtung, die außerhalb des Probengefäßes angeordnet ist, so daß die Schallwelleneinkopplung in die Probe durch die Probengefäßwand hindurch erfolgt. Die Erfindung zeichnet sich dadurch aus, daß die elektroakustische Wandlereinrichtung Schallwellen mit einer Frequenz von zumindest 100 kHz, vorzugsweise von 500 kHz bis 5 MHz, erzeugt, und daß Mittel zum Fokussieren der Schallwellen vorgesehen sind, die die Schallwellen auf ein Probenvolumen innerhalb des Probengefäßes von etwa 50 νl konzentrieren.
PCT/DE1999/001233 1998-05-07 1999-04-23 Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen WO1999058637A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US09/674,813 US6699711B1 (en) 1998-05-07 1999-04-23 Device and method for selective exposure of a biological sample to sound waves
EP99927719A EP1075507A2 (de) 1998-05-07 1999-04-23 Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19820466.3 1998-05-07
DE19820466A DE19820466C2 (de) 1998-05-07 1998-05-07 Vorrichtung und Verfahren zur gezielten Beaufschlagung einer biologischen Probe mit Schallwellen

Publications (2)

Publication Number Publication Date
WO1999058637A2 WO1999058637A2 (de) 1999-11-18
WO1999058637A3 true WO1999058637A3 (de) 2000-01-27

Family

ID=7866999

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1999/001233 WO1999058637A2 (de) 1998-05-07 1999-04-23 Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen

Country Status (4)

Country Link
US (1) US6699711B1 (de)
EP (1) EP1075507A2 (de)
DE (1) DE19820466C2 (de)
WO (1) WO1999058637A2 (de)

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Also Published As

Publication number Publication date
WO1999058637A2 (de) 1999-11-18
DE19820466A1 (de) 1999-11-18
DE19820466C2 (de) 2002-06-13
EP1075507A2 (de) 2001-02-14
US6699711B1 (en) 2004-03-02

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