EP0271448A2 - Behälter, bestimmt zur Aufnahme eines flüssigen Mediums - Google Patents

Behälter, bestimmt zur Aufnahme eines flüssigen Mediums Download PDF

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Publication number
EP0271448A2
EP0271448A2 EP87810725A EP87810725A EP0271448A2 EP 0271448 A2 EP0271448 A2 EP 0271448A2 EP 87810725 A EP87810725 A EP 87810725A EP 87810725 A EP87810725 A EP 87810725A EP 0271448 A2 EP0271448 A2 EP 0271448A2
Authority
EP
European Patent Office
Prior art keywords
wall
test piece
groove
liquid
container
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP87810725A
Other languages
English (en)
French (fr)
Other versions
EP0271448A3 (de
Inventor
Anthony Ringrose
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Intracel Corp
Original Assignee
Intracel Corp
Battelle Memorial Institute Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intracel Corp, Battelle Memorial Institute Inc filed Critical Intracel Corp
Publication of EP0271448A2 publication Critical patent/EP0271448A2/de
Publication of EP0271448A3 publication Critical patent/EP0271448A3/de
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/50—Mixing receptacles

Definitions

  • the present invention relates to a test tube intended to contain a liquid medium in which a phenomenon of cavitation must be generated by transmission of ultrasound using an ultrasonic transducer brought into contact with the external face of the wall of this enclosure. .
  • a container intended to contain a liquid, a portion of the bottom of which is connected to the rest of the container by a rubber diaphgragm which makes this portion free to vibrate relative to the wall of the container.
  • This portion is kinematically integral with an oscillator intended to communicate ultrasonic frequency vibrations to it.
  • the face of this portion of the bottom in contact with the liquid in the form of a very flared cone, the top of which is directed towards the outside of the container.
  • DE 952.763 shows a pipe, a portion of the wall of which is made more elastic and is connected to an ultrasonic transducer, to communicate vibrations to the liquid contained in the pipe in order to homogenize it.
  • the transmission of ultrasonic energy through the wall of a test tube with an energy density sufficient to cause cavitation in the liquid medium by simple pressing of the ultrasonic transducer poses various problems.
  • the test piece must be made of a material which has a sufficiently low acoustic impedance to transmit the vibrations.
  • the energy necessary to generate the cavitation must not be capable of damaging the test piece by breaking it or by melting it locally and superficially.
  • it is also important not to cause excessive heating of the liquid to be analyzed.
  • the samples to be analyzed are generally of the order of a microliter, the test pieces are very small. In the case where the mixing of liquids must be carried out on an automatic analysis apparatus, it is obvious that the transducer used must be sufficiently small and therefore of low power.
  • the object of the present invention is to provide a solution which makes it possible to meet all of the required conditions.
  • the subject of this invention is a test tube intended to receive a liquid medium according to claim 1.
  • the proposed solution allows the adaptation of the ultrasonic mixing technique to test pieces using a low power transducer, without rigid connection either between the transducer and the test piece, or between the test piece and its support, which allows the use of this solution with an automatic analysis device in which the test pieces are removably fixed to a transfer member moving the test pieces opposite different stations corresponding to the different stages of analysis of the sample contained in the test tube.
  • This solution also solves the problem of contamination of the samples, as well as that relating to the integrity of the volume of sample and of reagent placed in the test piece. Given the low energy required with the proposed solution to generate cavitation, there is no risk of damaging the test piece and excessively heating its contents.
  • the width of the side of the groove open towards the inside of the test piece and therefore causing the portion of liquid contained in this groove to communicate with the rest of the liquid contained in the test piece must not be greater at the depth of this groove. If this groove is triangular, or conical, the angle must not exceed 60 °.
  • Figs 1 to 3 consists of a tubular element 1 of rectangular section and widening slightly upwards, intended to receive a sample to be analyzed with its appropriate reagent (s).
  • the side wall 2 has a portion 3 of square shape with rounded angles in which the wall is thinned in order to give it a certain elasticity.
  • This thinned portion 3 surrounds a circular portion 4 similarly thickness than that of the wall surrounding the thinned portion 3.
  • the walls of this tubular element 1 are relatively rigid, they are made of polystyrene or polyacrylic injected 0.8 mm thick, while the thinned portion 3 has a thickness 0.3 mm and a minimum width of 2 mm, the circular portion 4 has 2 mm in diameter. These dimensions are not critical and can be changed in fairly large proportions.
  • the thinned portion 3 could have an annular shape. It essentially serves to ensure good contact between the circular portion 4 and the ultrasonic transducer intended to press against it. This intimate contact between the face of this circular portion 4 and the sonotrode is indeed an essential condition for the transmission of ultrasonic waves.
  • a groove 5 is formed on the internal face of the wall of this tubular enclosure 1 at the center of the circular portion 4.
  • the role of this groove is decisive for inducing cavitation in the liquid medium, and is all the more decisive since it has made it possible to demonstrate that it was possible to induce cavitation without the thinned portion 3, provided that there is good contact between the sonotrode and the external face of the tubular element 1, opposite the groove 5.
  • This therefore proves the predominant role of this groove 5 over that of the elastic portion 3 , which only seems to have the role of ensuring good contact with the sonotrode by allowing the external face of the portion 4 a certain angular displacement if the end of this sonotrode is not perfectly parallel to its external face.
EP87810725A 1986-12-11 1987-12-08 Behälter, bestimmt zur Aufnahme eines flüssigen Mediums Withdrawn EP0271448A3 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH4931/86 1986-12-11
CH4931/86A CH667599A5 (fr) 1986-12-11 1986-12-11 Enceinte destinee a contenir un milieu liquide.

Publications (2)

Publication Number Publication Date
EP0271448A2 true EP0271448A2 (de) 1988-06-15
EP0271448A3 EP0271448A3 (de) 1989-12-27

Family

ID=4285026

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87810725A Withdrawn EP0271448A3 (de) 1986-12-11 1987-12-08 Behälter, bestimmt zur Aufnahme eines flüssigen Mediums

Country Status (4)

Country Link
EP (1) EP0271448A3 (de)
JP (1) JPS6427649A (de)
CH (1) CH667599A5 (de)
DK (1) DK648587A (de)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0337690A1 (de) * 1988-04-08 1989-10-18 Amoco Corporation Verfahren zur Bereitung von Nukleinsäure für Hybridisierung
WO1999058637A2 (de) * 1998-05-07 1999-11-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen
FR2791697A1 (fr) * 1999-04-01 2000-10-06 Biomerieux Sa Appareil et procede de lyse par ultrasons de cellules biologiques
WO2000073413A2 (en) * 1999-05-28 2000-12-07 Cepheid Apparatus and method for cell disruption
US6431476B1 (en) 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
US6440725B1 (en) 1997-12-24 2002-08-27 Cepheid Integrated fluid manipulation cartridge
US6664104B2 (en) 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
US6818185B1 (en) 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
US8268603B2 (en) 1999-05-28 2012-09-18 Cepheid Apparatus and method for cell disruption
US8441629B2 (en) 2009-04-15 2013-05-14 Biocartis Sa Optical detection system for monitoring rtPCR reaction
US8512637B2 (en) 2009-05-06 2013-08-20 Biocartis Sa Device for cutting a sample carrier
US8641971B2 (en) 2009-04-14 2014-02-04 Biocartis Sa HIFU induced cavitation with reduced power threshold
US8815521B2 (en) 2000-05-30 2014-08-26 Cepheid Apparatus and method for cell disruption
US9073053B2 (en) 1999-05-28 2015-07-07 Cepheid Apparatus and method for cell disruption
US9079182B2 (en) 2009-04-15 2015-07-14 Biocartis Nv Protection of bioanalytical sample chambers

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE952763C (de) * 1952-07-23 1956-11-22 Siemens Ag Vorrichtung zum Homogenisieren od. dgl. von Fluessigkeiten, insbesondere von Milch, durch Schwingungsbehandlung
US2919215A (en) * 1952-02-21 1959-12-29 Thermofrigor Sa Apparatus for vibrating liquids
US3542345A (en) * 1968-06-13 1970-11-24 Ultrasonic Systems Ultrasonic vials and method and apparatus for mixing materials in same
US3567185A (en) * 1968-10-03 1971-03-02 Shell Oil Co Fluid resonator system
US3614069A (en) * 1969-09-22 1971-10-19 Fibra Sonics Multiple frequency ultrasonic method and apparatus for improved cavitation, emulsification and mixing
US3633877A (en) * 1969-09-11 1972-01-11 Albert G Bodine Inductive cavitator
FR2344329A1 (fr) * 1976-03-18 1977-10-14 Deberghe & Lafaye Procede et dispositif permettant de modifier les caracteristiques d'un fluide
US4571087A (en) * 1983-03-22 1986-02-18 Board Of Regents, University Of Texas System Array sonicator apparatus for automated sample preparation

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2919215A (en) * 1952-02-21 1959-12-29 Thermofrigor Sa Apparatus for vibrating liquids
DE952763C (de) * 1952-07-23 1956-11-22 Siemens Ag Vorrichtung zum Homogenisieren od. dgl. von Fluessigkeiten, insbesondere von Milch, durch Schwingungsbehandlung
US3542345A (en) * 1968-06-13 1970-11-24 Ultrasonic Systems Ultrasonic vials and method and apparatus for mixing materials in same
US3567185A (en) * 1968-10-03 1971-03-02 Shell Oil Co Fluid resonator system
US3633877A (en) * 1969-09-11 1972-01-11 Albert G Bodine Inductive cavitator
US3614069A (en) * 1969-09-22 1971-10-19 Fibra Sonics Multiple frequency ultrasonic method and apparatus for improved cavitation, emulsification and mixing
FR2344329A1 (fr) * 1976-03-18 1977-10-14 Deberghe & Lafaye Procede et dispositif permettant de modifier les caracteristiques d'un fluide
US4571087A (en) * 1983-03-22 1986-02-18 Board Of Regents, University Of Texas System Array sonicator apparatus for automated sample preparation

Cited By (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0337690A1 (de) * 1988-04-08 1989-10-18 Amoco Corporation Verfahren zur Bereitung von Nukleinsäure für Hybridisierung
US6440725B1 (en) 1997-12-24 2002-08-27 Cepheid Integrated fluid manipulation cartridge
WO1999058637A2 (de) * 1998-05-07 1999-11-18 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen
WO1999058637A3 (de) * 1998-05-07 2000-01-27 Fraunhofer Ges Forschung Vorrichtung und verfahren zur gezielten beaufschlagung einer biologischen probe mit schallwellen
US6699711B1 (en) * 1998-05-07 2004-03-02 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Device and method for selective exposure of a biological sample to sound waves
US6887693B2 (en) 1998-12-24 2005-05-03 Cepheid Device and method for lysing cells, spores, or microorganisms
US6987018B2 (en) 1998-12-24 2006-01-17 Cepheid Container for holding cells or viruses for disruption
US6686195B1 (en) 1999-04-01 2004-02-03 Biomerieux S.A. Method and apparatus for ultrasonic lysis of biological cells
FR2791697A1 (fr) * 1999-04-01 2000-10-06 Biomerieux Sa Appareil et procede de lyse par ultrasons de cellules biologiques
EP1466966A1 (de) * 1999-04-01 2004-10-13 Biomerieux S.A. Apparat und Prozess für Lysis durch biologische Ultraschall der Zellen
WO2000060049A1 (fr) * 1999-04-01 2000-10-12 Biomerieux S.A. Appareil et procede de lyse par ultrasons de cellules biologiques
US9789481B2 (en) 1999-05-28 2017-10-17 Cepheid Device for extracting nucleic acid from a sample
US8709363B2 (en) 1999-05-28 2014-04-29 Cepheid Cartridge for conducting a chemical reaction
US6783736B1 (en) 1999-05-28 2004-08-31 Cepheid Cartridge for analyzing a fluid sample
WO2000073413A2 (en) * 1999-05-28 2000-12-07 Cepheid Apparatus and method for cell disruption
US6818185B1 (en) 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
US9943848B2 (en) 1999-05-28 2018-04-17 Cepheid Apparatus and method for cell disruption
US6881541B2 (en) 1999-05-28 2005-04-19 Cepheid Method for analyzing a fluid sample
WO2000073413A3 (en) * 1999-05-28 2001-01-25 Cepheid Apparatus and method for cell disruption
US6391541B1 (en) 1999-05-28 2002-05-21 Kurt E. Petersen Apparatus for analyzing a fluid sample
US8168442B2 (en) 1999-05-28 2012-05-01 Cepheid Cartridge for conducting a chemical reaction
US8268603B2 (en) 1999-05-28 2012-09-18 Cepheid Apparatus and method for cell disruption
US9322052B2 (en) 1999-05-28 2016-04-26 Cepheid Cartridge for conducting a chemical reaction
US9073053B2 (en) 1999-05-28 2015-07-07 Cepheid Apparatus and method for cell disruption
US8580559B2 (en) 1999-05-28 2013-11-12 Cepheid Device for extracting nucleic acid from a sample
US6878540B2 (en) 1999-06-25 2005-04-12 Cepheid Device for lysing cells, spores, or microorganisms
US6664104B2 (en) 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
US6431476B1 (en) 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
US8815521B2 (en) 2000-05-30 2014-08-26 Cepheid Apparatus and method for cell disruption
US8641971B2 (en) 2009-04-14 2014-02-04 Biocartis Sa HIFU induced cavitation with reduced power threshold
US8986612B2 (en) 2009-04-14 2015-03-24 Biocartis Nv HIFU induced cavitation with reduced power threshold
US9097626B2 (en) 2009-04-14 2015-08-04 Biocartis Nv HIFU induced cavitation with reduced power threshold
US9079182B2 (en) 2009-04-15 2015-07-14 Biocartis Nv Protection of bioanalytical sample chambers
US8441629B2 (en) 2009-04-15 2013-05-14 Biocartis Sa Optical detection system for monitoring rtPCR reaction
US8512637B2 (en) 2009-05-06 2013-08-20 Biocartis Sa Device for cutting a sample carrier

Also Published As

Publication number Publication date
DK648587A (da) 1988-06-12
CH667599A5 (fr) 1988-10-31
JPS6427649A (en) 1989-01-30
EP0271448A3 (de) 1989-12-27
DK648587D0 (da) 1987-12-10

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