WO1999029905B1 - Methode de detection de l'apoptose a l'aide d'oligonucleotides marques par fret - Google Patents

Methode de detection de l'apoptose a l'aide d'oligonucleotides marques par fret

Info

Publication number
WO1999029905B1
WO1999029905B1 PCT/US1998/026432 US9826432W WO9929905B1 WO 1999029905 B1 WO1999029905 B1 WO 1999029905B1 US 9826432 W US9826432 W US 9826432W WO 9929905 B1 WO9929905 B1 WO 9929905B1
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotide
nucleotide sequence
moiety
detectable
energy
Prior art date
Application number
PCT/US1998/026432
Other languages
English (en)
Other versions
WO1999029905A3 (fr
WO1999029905A2 (fr
Inventor
William M James
Irina A Nazarenko
Original Assignee
Intergen Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intergen Company filed Critical Intergen Company
Priority to JP2000524476A priority Critical patent/JP2001526052A/ja
Priority to EP98963108A priority patent/EP1036201A2/fr
Priority to CA002314225A priority patent/CA2314225A1/fr
Publication of WO1999029905A2 publication Critical patent/WO1999029905A2/fr
Publication of WO1999029905A3 publication Critical patent/WO1999029905A3/fr
Publication of WO1999029905B1 publication Critical patent/WO1999029905B1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes d'amplification en chaîne par polymérase induite par ligase (LM-PCR) utilisées pour détecter des fragments cibles d'acides nucléiques à double brin tels que ceux qui sont générés lors du processus apoptosique. Selon ces méthodes, un oligonucléotide détectable est incorporé dans la cible. Cet oligonucléotide détectable renferme la fraction donneur et/ou la fraction receveur d'une paire de transfert d'énergie moléculaire (MET). Un exemple de paire MET est une paire de transfert par résonance d'énergie de fluorescence (FRET) constituée d'une fraction fluorophore (donneur) et d'une fraction extinctrice de luminescence (receveur). La fraction donneur de la paire MET émet de l'énergie détectable telle que de la lumière seulement lorsque l'oligonucléotide détectable est incorporé dans la cible. Selon ces méthodes, un oligonucléotide lieur-amorce est attaché à un oligonucléotide d'aide à la liaison, ou un oligonucléotide lieur-amorce renfermant une séquence d'aide à la liaison est lié à l'extrémité 5' de chacun des brins d'un fragment d'acides nucléiques à double brin renfermant soit une extrémité franche soit un surplomb terminal. Après cette étape de liaison, un oligonucléotide détectable capable d'être attaché au complément de l'oligonuléotide lieur-amorce est incorporé dans la cible par réactions catalysées par polymérase. Selon un autre mode de réalisation, l'oligonucléotide lieur-amorce est également un oligonucléotide détectable. Eventuellement, la cible marquée par l'oligonucléotide détectable est ensuite amplifiée, l'oligonucléotide détectable étant incorporé au produit d'amplification. La cible est détectée par détection de l'énergie émise par le groupe donneur de l'oligonucléotide détectable.
PCT/US1998/026432 1997-12-12 1998-12-11 Methode de detection de l'apoptose a l'aide d'oligonucleotides marques par fret WO1999029905A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2000524476A JP2001526052A (ja) 1997-12-12 1998-12-11 Ｆｒｅｔ標識オリゴヌクレオチドを用いたアポトーシスを検出する方法
EP98963108A EP1036201A2 (fr) 1997-12-12 1998-12-11 Methode de detection de l'apoptose a l'aide d'oligonucleotides marques par fret
CA002314225A CA2314225A1 (fr) 1997-12-12 1998-12-11 Methode de detection de l'apoptose a l'aide d'oligonucleotides marques par fret

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6943497P 1997-12-12 1997-12-12
US60/069,434 1997-12-12

Publications (3)

Publication Number Publication Date
WO1999029905A2 WO1999029905A2 (fr) 1999-06-17
WO1999029905A3 WO1999029905A3 (fr) 1999-08-26
WO1999029905B1 true WO1999029905B1 (fr) 1999-10-21

Family

ID=22088952

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/026432 WO1999029905A2 (fr) 1997-12-12 1998-12-11 Methode de detection de l'apoptose a l'aide d'oligonucleotides marques par fret

Country Status (4)

Country Link
EP (1) EP1036201A2 (fr)
JP (1) JP2001526052A (fr)
CA (1) CA2314225A1 (fr)
WO (1) WO1999029905A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10048009A1 (de) * 2000-09-26 2002-04-11 Cytonet Gmbh & Co Kg Verfahren zum Nachweis von Pilzinfektionen
JP2004530410A (ja) * 2000-10-24 2004-10-07 インタージェン カンパニー 特異的ヌクレオチド配列の検出
CN1556224B (zh) * 2004-01-08 2012-08-22 湖南大学 核酸片段检测系统及其在核酸分析及相关酶研究中的应用
US8679741B2 (en) 2006-05-31 2014-03-25 Sequenom, Inc. Methods and compositions for the extraction and amplification of nucleic acid from a sample
WO2010115016A2 (fr) 2009-04-03 2010-10-07 Sequenom, Inc. Compositions constituées d'une préparation d'acides nucléiques et procédés associés
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
CN109182479A (zh) * 2018-07-27 2019-01-11 广州医科大学附属第医院 一种实时荧光定量pcr通用型荧光探针及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843658A (en) * 1995-03-10 1998-12-01 Hamamatsu Photonics K.K. Method of measuring oligonucleotide decomposing activity
WO1997007205A1 (fr) * 1995-08-11 1997-02-27 Novo Nordisk A/S Proceder pour preparer des variants polypeptidiques
JP3439221B2 (ja) * 1995-12-18 2003-08-25 ワシントン ユニヴァーシティ 蛍光共鳴エネルギー移動を利用する核酸分析方法
DE69738687D1 (de) * 1996-04-12 2008-06-26 Phri Properties Inc Sonden, kits und assays
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon

Also Published As

Publication number Publication date
WO1999029905A3 (fr) 1999-08-26
CA2314225A1 (fr) 1999-06-17
WO1999029905A2 (fr) 1999-06-17
EP1036201A2 (fr) 2000-09-20
JP2001526052A (ja) 2001-12-18

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