WO1999027131A3 - Molecule d'acide nucleique liee a la matrice et topologiquement fixee - Google Patents

Molecule d'acide nucleique liee a la matrice et topologiquement fixee Download PDF

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Publication number
WO1999027131A3
WO1999027131A3 PCT/DE1998/003466 DE9803466W WO9927131A3 WO 1999027131 A3 WO1999027131 A3 WO 1999027131A3 DE 9803466 W DE9803466 W DE 9803466W WO 9927131 A3 WO9927131 A3 WO 9927131A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
fixed matrix
bonded nucleic
matrix bonded
Prior art date
Application number
PCT/DE1998/003466
Other languages
German (de)
English (en)
Other versions
WO1999027131A2 (fr
Inventor
Claas Junghans
Burghardt Wittig
Original Assignee
Claas Junghans
Burghardt Wittig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Claas Junghans, Burghardt Wittig filed Critical Claas Junghans
Priority to EP98963380A priority Critical patent/EP0975808A2/fr
Publication of WO1999027131A2 publication Critical patent/WO1999027131A2/fr
Publication of WO1999027131A3 publication Critical patent/WO1999027131A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne des molécules d'acide nucléique qui sont liées à une surface de telle façon que l'énergie de spire et la superhélicité ne puissent s'en échapper. L'invention concerne également un procédé permettant d'analyser de tels effets enzymatiques qui modifient l'état topologique de l'ADN. De telles molécules d'acide nucléique et de tels procédés peuvent être utilisés en recherche pharmaceutique dans la recherche automatique de cytostatiques ou d'antibiotiques.
PCT/DE1998/003466 1997-11-21 1998-11-19 Molecule d'acide nucleique liee a la matrice et topologiquement fixee WO1999027131A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP98963380A EP0975808A2 (fr) 1997-11-21 1998-11-19 Molecule d'acide nucleique liee a la matrice et topologiquement fixee

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19753182.2 1997-11-21
DE19753182A DE19753182A1 (de) 1997-11-21 1997-11-21 Topologisch fixierte matrixgebundene Nukleinsäure

Publications (2)

Publication Number Publication Date
WO1999027131A2 WO1999027131A2 (fr) 1999-06-03
WO1999027131A3 true WO1999027131A3 (fr) 1999-10-07

Family

ID=7850347

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/003466 WO1999027131A2 (fr) 1997-11-21 1998-11-19 Molecule d'acide nucleique liee a la matrice et topologiquement fixee

Country Status (3)

Country Link
EP (1) EP0975808A2 (fr)
DE (1) DE19753182A1 (fr)
WO (1) WO1999027131A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2412513A1 (fr) * 2000-06-22 2001-12-27 San Diego State University Foundation Modulateurs de recombinaison et leurs methodes de production et d'utilisation
GB0424953D0 (en) * 2004-11-11 2004-12-15 Plant Bioscience Ltd Assay
CA2665783C (fr) 2006-10-13 2015-12-15 Novosom Ag Ameliorations des liposomes amphoteres, ou en relation avec ceux-ci, procede de formulation d'un liposome amphotere et procede de charge d'un liposome amphotere
WO2008074487A2 (fr) 2006-12-19 2008-06-26 Novosom Ag Lipides et assemblages lipidiques comprenant des éléments activateurs de transfection
DE102007029471A1 (de) 2007-06-20 2008-12-24 Novosom Ag Neue fakultativ kationische Sterole
JP5851399B2 (ja) 2009-07-09 2016-02-03 マリーナ バイオテック，インコーポレイテッド イミノ脂質を含む両性リポソーム

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0484472B1 (fr) * 1990-04-12 1997-07-16 SIGRIST, Hans, Dr. Procede pour l'immobilisation induite par la lumiere de biomolecules sur des surfaces chimiquement "inertes"
DE19648625A1 (de) * 1996-11-13 1998-05-14 Soft Gene Gmbh Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ARNDT-JOVIN D. ET AL.,: "Z-DNA binding and inhibition by GTP of Drosophila topoisomeraes II", BIOCHEMISTRY, vol. 32, - 1993, pages 4862 - 4872, XP002111474 *
GLIKIN G.C. ET AL.,: "Interactions of drosophila DNA topoisomerase II with left-handed Z-DNA in supercoiled minicircles", NUCLEIC ACIDS RESEARCH, vol. 19, no. 25, - 1991, pages 7139 - 7144, XP002111475 *
THOMAS T.J.& THOMAS T.: "Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides", NUCLEIC ACIDS RESEARCH, vol. 17, - 1989, pages 3795 - 3810, XP002111473 *

Also Published As

Publication number Publication date
EP0975808A2 (fr) 2000-02-02
WO1999027131A2 (fr) 1999-06-03
DE19753182A1 (de) 1999-07-29

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