WO1999014598A1 - Systeme de detection pour aliments - Google Patents

Systeme de detection pour aliments Download PDF

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Publication number
WO1999014598A1
WO1999014598A1 PCT/US1998/019167 US9819167W WO9914598A1 WO 1999014598 A1 WO1999014598 A1 WO 1999014598A1 US 9819167 W US9819167 W US 9819167W WO 9914598 A1 WO9914598 A1 WO 9914598A1
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WO
WIPO (PCT)
Prior art keywords
food
antibodies
contamination detector
food contamination
antigen
Prior art date
Application number
PCT/US1998/019167
Other languages
English (en)
Inventor
Robert M. Goldsmith
Catherine Goldsmith
James G. Woodaman
Douglas L. Park
Carlos E. Ayala
Original Assignee
California South Pacific Investors
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California South Pacific Investors filed Critical California South Pacific Investors
Priority to JP2000512083A priority Critical patent/JP2001516883A/ja
Priority to EP98948221A priority patent/EP1018013A1/fr
Priority to CA002303384A priority patent/CA2303384A1/fr
Priority to AU94839/98A priority patent/AU9483998A/en
Publication of WO1999014598A1 publication Critical patent/WO1999014598A1/fr
Priority to NO20001297A priority patent/NO20001297L/no

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody

Definitions

  • the field of the present invention is the detection of substances or contaminants in edible consumer products .
  • Contamination of food can come from a variety of sources and the type of contamination possible is often dependent on the type of food involved.
  • Most animal derived food products, such as raw meat, are exposed to contamination before, during, or after processing. In most cases, contamination is minor and, if the food is prepared properly, may not pose a serious threat to the consumer.
  • the contamination of food is generally low, i.e. few bacteria per gram of food, if the food is not stored under satisfactory conditions, or if the food is stored for long periods of time, contaminants, such as bacteria, may grow to become a serious threat to the eventual consumer. Even if the food products reach the market in an acceptable condition, subsequent mistreatment by the consumer may lead to the development of food contamination .
  • Food products are often "mass produced” and sold at retail outlets in prepackaged containers.
  • Such packages typically include a styrofoam, plastic or cardboard tray which supports the food product.
  • the tray and food are sealed in a transparent plastic wrap material and a liner lies between the food product and the inside bottom of the tray.
  • a bar code is often used on the products for scanning at the check-out register, to reduce errors in totaling purchases and for stock control.
  • the bar code comprises a series or pattern of bars, which represent a number identifying the product.
  • Any solution to this problem should be relatively inexpensive and able to detect a number of agents capable of causing illness. It should also be simple to read so that consumers, who do not have access to sophisticated testing equipment or specialized knowledge, can readily determine if the products they have purchased are free from contamination .
  • a first, separate aspect of the present invention is directed to an improvement on the previously described methods of detecting contaminants in food by providing for the directional flow of the fluids or juices bringing these fluids or juices into contact with a detecting element.
  • Such directional flow advantageously enhances the sensitivity and reliability of the detection system, permitting the analysis of a larger volume and more representative sample of juices.
  • a second, separate aspect of the present invention is directed to an indicator dependent on the directional capillary flow of the fluids wherein contamination is to be detected.
  • the indicator includes a first layer impermeable support, a second capillary layer having an immunobead solution pad and a detection area, this second layer providing for the directional flow of juices and being supported by the impermeable support, and a third impermeable cover film layer, having a impermeable cover film, for covering the capillary layer, such that the capillary layer rests between the impermeable support layer and the impermeable cover film layer.
  • a third, separate aspect of the present invention is directed to a code indicia which may be modified or rendered unreadable by a contamination detector providing for the directional capillary flow of the fluids where contamination is to be detected.
  • the code indicia may include at least one, or preferably at least two, bar codes, at least one of which is modified or rendered unreadable in the presence of the antigen or antigens the indicator is intended to detect.
  • the code indicia may be characterized by utilizing, with the indicator, a first bar code for which the change in appearance of the indicator in the presence of an antigen or antigens makes the first bar code unreadable by a bar code reader, and a second bar code for which the change in appearance of the indicator in the presence of an antigen or antigens makes the second bar code readable by a bar code reader.
  • a fourth, separate aspect of the present invention is directed to an indicator dependent on the directional capillary flow of the fluids wherein contamination is to be detected.
  • the indicator includes a first layer impermeable support, a second capillary layer having an immunobead solution pad and at least two detection areas, this second layer providing for the directional flow of juices and being supported by the impermeable support, and a third impermeable cover film layer, having a impermeable cover film, for covering the capillary layer, such that the capillary layer rests between the impermeable support layer and the impermeable cover film layer and each detection area is in alignment with a window in the cover film and a code indicia .
  • a fifth, separate aspect of the present invention is directed to a method for the detection of food contamination for use with a food container adapted for containing a food product which produces juices.
  • the juices are sequentially drawn through a first absorbent material into and through an immunobead solution pad, including a first set of antibodies specific to an antigen to be detected, the first set of antibodies being linked to a light absorbing material, and through a substrate, including a second set of antibodies specific to the antigen to be detected, the first set of antibodies, or to the combination thereof, said second set of antibodies being bound to a membrane in a detection area.
  • Figure 1 is a longitudinal cross sectional view of a contamination detector for use in food safety called the Food Sentinel SystemTM.
  • Figure 2 is a top plan view of the Food Sentinel SystemTM.
  • Figure 3 is a top plan view of an alternative preferred embodiment of the Food Sentinel SystemTM.
  • Figure 4 is a longitudinal cross-section schematic of an alternative arrangement for a contamination detector within the scope of the present invention.
  • the invention is based on the solid-phase immunobead assay and antibody sandwich principles modified to faciliate the continuous flow of product juices and contaminants.
  • the contaminants may be microorganisms, bacteria, viruses, toxins or any other immunogenic materials or substances, and are herein referred to as "antigen" or "antigens.”
  • the assay may preferably be linked to a uniform product code bar system.
  • the antigen or antigens are detected as they pass through the Food Sentinel SystemTM by binding to antigen- specific colored immunobead complex which then migrates to be captured by a second specific antibody.
  • the second specific antibody is attached to a membrane forming part of the uniform product code bar system. The presence of the contaminating antigen is evident by a localized color bar formation.
  • the appearance of a bar indicating the presence of antigen can then be determined by simple optical scanning of the bar code attached to the product or by visually observing the appearance of a symbol, a letter, a word, or the like.
  • the scanner can be programmed to read the type of contamination, product, and record the date and location of the reading.
  • the membranes are designated to allow entry of antigen, prevent the entrance of interfering substances, and maintain the complex formed between the immunobead and the antigen to be detected inside the system.
  • the Food Sentinel System remains closely associated with the product, thus allowing for early detection and tracking product safety from slaughter or processing/packaging plant to the consumer.
  • the presence of a visual symbol on or in addition to the code indicia may also permit the determination of product safety in the absence of scanning devices, such as in the consumer's home.
  • FIG 1 is a longitudinal cross sectional view of a contamination detector for use in food safety called the Food Sentinel SystemTM.
  • the Food Sentinel SystemTM may be used in a preformed well of a food package as described in the above referenced patent and applications or, advantageously, it may be embedded in the food package itself.
  • the Food Sentinel SystemTM may be embedded or closely associated with a liner, which commonly is present in the package of food products and lies between the tray and the food, such that juices collected in the liner are drawn into the Food Sentinel SystemTM.
  • the components of the Food Sentinel SystemTM are substantially the same.
  • a cover film 2 composed of mylar acetate or some other type of transparent nonporous film contains a code indicia (not shown) such as the ubiquitous UPC bar code which codes for a particular food product that is contained in the package in which the particular Food Sentinel SystemTM is associated.
  • the bars of the bar code may be printed onto the film and the light reflective spaces may be created by using adhesive white paper 4 or some other method such as painting or staining the back of the cover film 2, provided that light may be reflected to a bar code reading device such as a laser bar code scanner, light pen, CCD or other such device that can detect the bars and spaces of the bar code.
  • the cover film 2 preferably has a window 6 which contains at least one space of the bar code. Window 6 is aligned with detection area 26 (See Figure 2) of substrate 8.
  • detection area 26 See Figure 2
  • the prepackaged food product will be shipped to a retail outlet such as a grocery store which will attach its own bar code label (cover film 2) to and in alignment with detection area 26.
  • cover film 2 When the Food Sentinel SystemTM detects contamination, the light reflectivity of detection area 26 will change from being light reflecting to light absorbing.
  • UPC bar codes that means a color change from white to black.
  • other colors, shades, or metallic lusters may be used to accomplish this effect.
  • detection area becomes light absorbing, a bar code reading device will be unable to successfully decode the code indicia of cover film 2 and contamination can be detected.
  • Further detection methods that may be used with the present invention are described in the above referenced patent and patent applications. For example, these may include symbols, letters, words, and the like, which become readable or unreadable (depending on the implementation) , in the presence of the antigen or antigens to be detected.
  • the indicator area 26 may be placed such that it obliterates part of the product identification code rendering it incomplete to avoid the accidental checkout of contaminated product, or it may modify it, such that it represents a different code.
  • One or several indicator areas may be present in a substrate 8.
  • the code indicia may be characterized by utilizing, with the indicator, a first bar code for which the change in appearance of the indicator in the presence of antigen makes the first bar code unreadable by a bar code reader, and a second bar code for which the change in appearance of the indicator in the presence of antigen makes the second bar code readable by a bar code reader.
  • the indicator area 26 could include a number which appears upon contamination to complete a numeric code corresponding to the data code by the contamination detecting bar code.
  • migration film 10 which is composed of mylar acetate or some other transparent nonporous film.
  • a support 12 which can be a plastic strip, a film such as mylar acetate or some other nonporous film or substance.
  • FIG 2 is a top plan view of the Food Sentinel SystemTM.
  • the Food Sentinel SystemTM functions as follows. Liquids associated with a food product such as blood and juices from meat and fish, or liquids packaged with the food or the food itself, such as milk and juices, enter an inflow orifice 14 of the Food Sentinel SystemTM. The liquid passes through a protective filter 16 which filters out larger materials such as proteins and clumped dead cells . The liquid then passes through a selective filter 18 that filters out other materials and molecules larger than the toxin of interest such as ciguatoxin, brevetoxin or other toxins associated with food poisoning, or a bacteria of interest such as E. coli, Salmonella, Listeria, Campylobacter or other bacteria associated with food poisoning. The filters are not considered essential. Moreover, other means of filtering, well known in the art, may be used.
  • Absorbent pad 20 includes a primary absorbent material 22.
  • "absorbent pad” or “absorbent material” means any material providing for the directional capillary flow of juices or fluids. These materials include, for example, blotting paper, Whatman paper, structures having thin capillary conduits made of any of the several well known in the art materials that do not detrimentally affect the sample travelling therein, and the like.
  • the filtered liquid passes through the primary absorbent material 22, it passes through the immunobead solution pad 24.
  • Immunobead solution pad 24 contains monoclonal anti- odies bound to colored latex microspheres, and is created by standard techniques as later described herein.
  • the immunobead solu- ion pad may have one type of monoclonal antibody or multiple types of monoclonal antibodies to ensure detection of a single antigen.
  • the immunobead solution pad may have multiple types of monoclonal antibodies to detect multiple antigens.
  • the immunobead solution pad may use polyclonal antibodies instead of and/or in addition to monoclonal antibodies. The preparation of such antigen-specific antibodies is well known in the art. In some cases it may be necessary to conjugate a toxin antigen to a protein to "mask" the toxicity of the antigen.
  • Substrate 8 is a membrane, such as Immobilon-P, to which another monoclonal antibody is bound to a detection area 26.
  • a polyclonal antibody may be used on substrate 8 to form detection area 26.
  • the detection area 26 may encompass the entire area of the substrate 8, or only a portion of it.
  • Antibodies on the substrate 8 can be specific to the antibodies contained in the immunobead solution pad, the antigen or antigens, the antigen to be detected bound to the antibodies contained in the immunobead solution pad, or mixtures thereof.
  • the first and second antibodies could be the same, they are preferably different.
  • the second antibody preferably recognizes different antigenic determinants on the antigen than the first antibody. Consequently, as the filtered liquid passes across substrate 8, the antigens bound to antibodies bound to the colored latex microsphere are captured in detection area 26. As the number of antibodies bound to the colored latex microsphere and a toxin or bacteria increases, so does the color of detection area 26 so that eventually the light reflectivity of detection area 26 changes from light reflecting to light absorbing at which point contamination is considered to have occurred. The filtered liquid is then withdrawn by a secondary absorbent material 28.
  • FIG 3 is a top plan view of an alternative embodient of the Food Sentinel SystemTM.
  • This embodiment functions essentially the same as the embodiments shown in Figures 1 and 2 except that secondary absorbent material 28 also functions as a filter which prevents antibodies bound to a colored latex microsphere and antigen to pass beyond the substrate 8 area to the outflow orifice.
  • the membrane form- ng substrate 8 is channeled to allow the antibodies bound to a colored latex microsphere and a antigen to recirculate over the detection area to further enhance the sensitivity of the Food Sentinel SystemTM.
  • Figure 4 is a longitudinal cross-section schematic of an alternative arrangement for a contamination detector within the scope of the present invention.
  • a first layer includes an impermeable support 12, serving as a support for a second layer having an immunobead solution pad 24, described previously.
  • Two or more substrates 8 and 9 (two shown in the figure) , having detection areas 26 and 27, described previously, abut and are in contact with the immunobead solution pad 24 in the second layer.
  • a third layer has a cover film 2 composed of a transparent nonporous film that separates code indicia 40, such as the UPC bar codes shown 42 and 44, from the second layer.
  • the detection areas 26 and 27 are aligned with the window 6 or windows in the cover film 2 and the code indicia 40, 42, 44.
  • this alternative embodiment draws product juices through inflow orifice 14 into or onto the immunobead solution pad 24.
  • antigens if present, bind a first set of antibodies in the immunobead solution pad 24.
  • the fluid - now laden with the first set of antibodies bound to both a colored latex microsphere and an antigen - passes from the immunobead solution pad 24 onto the substrates 8 and 9.
  • the first set of antibodies bound to both a colored latex microsphere and an antigen are captured in detection areas 26 and 27 by a second set of antibodies specific to the antibodies contained in the immunobead solution pad, the antigen or antigens, the antigen bound to the antibodies contained in the immunobead solution pad, or mixtures thereof.
  • the number of antibodies bound to the colored latex microspheres and antigen increases, so does the color of detection areas 26 and 27, so that eventually the light reflectivity of detection areas 26 and 27 changes from light reflecting to light absorbing. At that point contamination is considered to have occurred.
  • the code indicia may have a first bar code 44 for which the change in appearance of the first indicator 27 in the presence of antigen makes the first bar code 44 unreadable by a bar code reader, and a second bar code 42 for which the change in appearance of the second indicator 26 in the presence of antigen makes the second bar code 42 readable by a bar code reader.
  • a first bar code 44 for which the change in appearance of the first indicator 27 in the presence of antigen makes the first bar code 44 unreadable by a bar code reader
  • a second bar code 42 for which the change in appearance of the second indicator 26 in the presence of antigen makes the second bar code 42 readable by a bar code reader.
  • Other alternatives explained in detail throughout this application, may also be used with this embodiment .
  • a Food Sentinel SystemTM for the detection of E. coli may be produced as described below.
  • Anti-E.coli 0157 :H7 polyclonal antibody 1 mg/mL, commercially available from Kirkegaard & Perry Laboratories, Inc., Gaithersburg, MD; catalog number 01-95-90.
  • Sodium phosphate buffer (SPB) 0.1 M, pH 6.0.
  • Second blocking solution consisting of 1 M glycine in PBS.
  • Immobilon-P (PVDF) membrane commercially available from Millipore Corporation, Bedford, MA; catalog No. IPVH 101 00. Methanol CH 3 OH HPLC grade, commercially available from Chempure, Houston, TX; catalog No. 831-296.
  • Immunobead pad made from dried alcohol swab material from Baxter Scientific Products, McGaw Park, IL; catalog B3062. Sucrose 50% solution in PBS. Deionized distilled (dd) water.
  • Plastic support S-PIA stick commercially available from Millipore Corp. Acetate film, as used in overhead transparencies. Absorbent paper Whatman # 42. Non-toxic glue stick, available commercially from Conros Corp., Detroit, MI Ross Stik # 06-074.
  • PBS (lOmM) standard solution may be prepared according to Harlow, E. and D. Lane, Antibodies: a laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY at 726 (1988) .
  • Antibody stock solution (1 mg/mL) may be prepared by rehydration with 50% glycerol solution, according to antibody manufacturer's instructions.
  • SPB may be prepared as indicated in "Protocols for Adsorbing Proteins one Polystyrene-Based Microspheres," by Bangs Laboratories, Inc., 9025 Technology Drive, Fishers, IN 46038-2886, telephone (317) 570-7020, Fax (317) 570-7034.
  • the immunobead solution may be prepared following the Sodium Phosphate/PBS/BSA method indicated in "Protocols for Adsorbing Proteins onto Polystyrene-Based Micrpspheres , " by Bangs Laboratories, Inc.
  • a 6 L final volume of immunobead solution may be prepared with 120 ⁇ L antibody solution, 300 ⁇ L black latex microspheres, and 5,580 ⁇ L SPB.
  • An immunobead pad for use in the present invention may be prepared as follows. Alcohol is evaporated from the Baxter alcohol swab (pad) . The pad is then rinsed by repeated immersion in dd water. The step may then be repeated in a new amount of dd water, and the pad dried in an oven at 37 °C. The dried pad is placed on a glass surface and wetted with 1% BSA using a pipettor, and then dried by suspending it from a corner inside an oven at 37 °C. The dried pad is placed on a glass surface and wetted with 50% sucrose using a pipettor, and then dried in an oven at 37 °C.
  • the dried pad is placed on a glass surface and wetted with immunobead solution using a pipettor. About 15 ⁇ L of immunobead solution is applied to a 0.9 cm x 0.9 cm section of blocked pad. The pad is again dried in an oven at 37 °C for about 10 minutes. The drying process is completed at 4 °C in a refrigerator.
  • the immunobead pads may be stored between sheets of non-adhesive paper tissue in plastic bags at 4 °C, and may be cut into appropriately sized pieces before Food Sentinel System assembly.
  • Preparation of Detection Area in the Substrate Immobilon-P membrane is pre-wetted as provided with the manufacturer's instructions. While the Immobilon-P membrane remains wet (opaque) , primary antibody is applied with a 10- ⁇ L pipettor (about 3 ⁇ L of anti-E. coli polyclonal antibody per membrane) in the shape desired for the detection area. For example, in the case of a bar code a vertical bar (0.7 cm x 0.2 cm) is applied.
  • the antibody solution preferably has a concentration of 20 ⁇ g/mL, PBS being used as the diluting solution. If the membrane partially dries (i.e.
  • the pre-wetting procedure should be repeated.
  • the applied antibody is dried by incubating the membrane over a KimwipeTM tissue in an oven at 37 °C for 10-15 minutes, or in a refrigerator at 4 °C for a longer period.
  • the membrane is then blocked by immersing the membrane in 1% BSA blocking solution for 30 minutes at room temperature. It is desirable that bubbles be prevented from forming on the surface of the membrane during the immersion procedure.
  • the membrane is then three times rinsed by immersions in PBS, each immersion lasting about five minutes. Excess PBS is eliminated by vertically placing the membrane on KimwipeTM tissue for about two minutes. The membrane is then dried on KimwipeTM tissue in an oven at 37 °C for 15 minutes or in the refrigerator at 4 °C for a longer period. Once dry, the blocked membrane is ready for assembly into the Food Sentinel SystemTM.
  • a Food Sentinel SystemTM may be assembled by cutting an S-PIA plastic strip into two 4.5 cm x 1 cm pieces, one of them with the attached membrane with antibody in detection area 26. Using an additional piece of S-PIA plastic strip (3 cm x 1 cm) as a basis, the two 4.5 cm x 1 cm pieces are glued end to end so that the strip returns to its original dimensions ( 9 cm x 1 cm), but with the membrane on the central part of the plastic strip. A thin line of non-toxic glue is applied along both edges of the strip, except on the membrane. A 0.9 cm x 0.9 cm piece of immunobead pad is attached to the strip so that one edge is flush against the membrane.
  • a 1.5 cm x 1 cm piece of absorbent paper is attached to the plastic strip so that it is flush against the free end of the immunobead pad, while a second 1.5 cm x 1 cm piece of absorbent paper is attached to the plastic strip so that it is flush against the free end of the membrane.
  • the plastic strip should have four components glued side by side in the following sequence: paper-membrane-pad-paper .
  • Glue may then be applied in a thin line against both edges of a 2 cm x 0.9 cm piece of acetate.
  • the acetate is glued so as to cover both the membrane and the pad.
  • the acetate may slightly overlap on the piece of absorbent paper next to the membrane or pad.
  • a bar code may be incorporated into the assembled Food Sentinel SystemTM by attaching a second piece of acetate (7 cm x 3 cm) with a printed Uniform Product Code bar code to one end of the plastic strip such that the bars to be modified, or made unreadable, match the area where the antibody was applied (detection area 26) on the underlying membrane (substrate 8) .
  • the Food Sentinel SystemTM may be stored under refrigeration.
  • a food contamination detector providing for the directional capillary flow of juices has been disclosed. While embodiments and applications of this invention have been shown and described, it would be apparent to those skilled in the art that many more modifications are possible without departing from the inventive concepts herein. For example, while the example describes the use antibodies against E.
  • the absorbent pad can be made of any material providing for the directional capillary flow of juices or fluids
  • the immunobead pad may be prepared using other materials and membranes well known in the art, as can the detection area. The invention, therefore is not to be restricted except in the spirit of the appended claims.

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Abstract

Cette invention concerne un indicateur de contamination des aliments, qui comprend une première couche servant de support imperméable, une deuxième couche et une troisième couche constituée d'un film de revêtement imperméable. La deuxième couche contient un matériau absorbant, qui permet l'écoulement direct de divers jus, un tampon pour une solution 'd'immuno-perles', et une zone de détection. La couche capillaire, qui repose sur la couche de support imperméable, est recouverte par la troisième couche en film de revêtement imperméable: de cette manière, la couche capillaire réside entre la couche de support imperméable et la troisième couche en film de revêtement imperméable.
PCT/US1998/019167 1997-09-15 1998-09-15 Systeme de detection pour aliments WO1999014598A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2000512083A JP2001516883A (ja) 1997-09-15 1998-09-15 フード・センチネル・システムｔｍ
EP98948221A EP1018013A1 (fr) 1997-09-15 1998-09-15 Systeme de detection pour aliments
CA002303384A CA2303384A1 (fr) 1997-09-15 1998-09-15 Systeme de detection d'aliments contamines
AU94839/98A AU9483998A (en) 1997-09-15 1998-09-15 Food sentinel system TM
NO20001297A NO20001297L (no) 1997-09-15 2000-03-13 Matvare-merkesystem

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5887397P 1997-09-15 1997-09-15
US60/058,873 1997-09-15

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WO1999014598A1 true WO1999014598A1 (fr) 1999-03-25

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PCT/US1998/019167 WO1999014598A1 (fr) 1997-09-15 1998-09-15 Systeme de detection pour aliments

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EP (1) EP1018013A1 (fr)
JP (1) JP2001516883A (fr)
CN (1) CN1278920A (fr)
AU (1) AU9483998A (fr)
CA (1) CA2303384A1 (fr)
NO (1) NO20001297L (fr)
WO (1) WO1999014598A1 (fr)

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WO2000020863A1 (fr) * 1998-10-06 2000-04-13 Verseau Group Detecteur de toxines
GB2427272A (en) * 2005-06-16 2006-12-20 Porvair Filtration Group Ltd Diagnostic device
WO2013115478A1 (fr) * 2012-02-03 2013-08-08 동국대학교 산학협력단 Kit d'emballage alimentaire permettant de détecter des bactéries

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DE102004052729A1 (de) * 2004-10-30 2006-05-04 Roche Diagnostics Gmbh Immunkomplex-spezifsicher Antikörper zur Reduktion des Nullwerts beim Nachweis von Antigen-spezifisch gebundenen Antikörpern einer bestimmten Immunglobulinklasse in Array-Testformaten
EP4109101A1 (fr) * 2021-06-24 2022-12-28 Technische Universität München Dispositif de test portable pour tester la susceptibilité d'une sonde biologique à une ou plusieurs substances bioactives

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GB2427272A (en) * 2005-06-16 2006-12-20 Porvair Filtration Group Ltd Diagnostic device
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CA2303384A1 (fr) 1999-03-25
CN1278920A (zh) 2001-01-03
AU9483998A (en) 1999-04-05
EP1018013A1 (fr) 2000-07-12
JP2001516883A (ja) 2001-10-02
NO20001297L (no) 2000-05-15
NO20001297D0 (no) 2000-03-13

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