WO2013115478A1 - Kit d'emballage alimentaire permettant de détecter des bactéries - Google Patents

Kit d'emballage alimentaire permettant de détecter des bactéries Download PDF

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WO2013115478A1
WO2013115478A1 PCT/KR2012/010799 KR2012010799W WO2013115478A1 WO 2013115478 A1 WO2013115478 A1 WO 2013115478A1 KR 2012010799 W KR2012010799 W KR 2012010799W WO 2013115478 A1 WO2013115478 A1 WO 2013115478A1
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Prior art keywords
bacteria
food
code
antibody
food packaging
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PCT/KR2012/010799
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English (en)
Korean (ko)
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성정석
이승주
강환수
김왕준
Original Assignee
동국대학교 산학협력단
한국보건산업진흥원
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Priority claimed from KR20120144496A external-priority patent/KR101494502B1/ko
Publication of WO2013115478A1 publication Critical patent/WO2013115478A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/50—Lipopolysaccharides; LPS

Definitions

  • the present invention relates to a food packaging kit comprising a bacterial specific antibody indicator and a QR code and / or bar code and a technology that can be used to identify food contamination in the field.
  • Gram negative bacillus Bacteria, the main cause of food poisoning, are typically divided into gram-negative bacteria and gram-positive bacteria.
  • Types of Gram negative bacillus include Salmonella, D. coli, Typhoid, Escherichia coli, Cholera, Pest, Gonococcus, Meningococci, Spiroteta and the like. In general, it has a strong resistance to triphenylmethane-based or acriflavin pigments and a strong resistance to surfactants. Gram-negative toxins are not easily destroyed by heating with endotoxins. It is a subject of cell antigens, but its immunity is weak.
  • Endotoxins unlike exotoxins, are lipid polysaccharides (LPS), which are components of the outer membrane of Gram-negative bacteria, which cause fever, shock, diarrhea, and sometimes internal bleeding or miscarriage.
  • Gram positive bacillus refers to bacteria that turn purple by gram staining, such as staphylococci and streptococci. Sensitive to pigments and drugs, toxins are easily destroyed when heated. Staphylococcus, streptococci, pneumococci, leprosy, diphtheria, tetanus, anthrax, actinomycetes, and the like are examples. Highly sensitive to pigments and drugs, metabolism requires amino acids and vitamins.
  • Gram-positive bacteria release cell exotoxins, which coagulase or leukocidin, which stimulate the intestinal cells to form blood clots with enterotoxins that cause diarrhea or food poisoning, Hemolysin, protease, and other enzymes kill the host cell (cytotoxin) and neurotoxin (neurotoxin) that interferes with the transmission of normal stimulation.
  • enterotoxins include cholera bacteria, E. coli, and dysentery bacteria. Staphylococcus aureus, Clostridium perfringens, E. coli, Salmonella, etc. There is this.
  • Food poisoning is a bacterial food poisoning that causes symptoms by infection by the bacteria itself or by a toxin produced by the bacteria, a natural poisoning food poisoning caused by animal or vegetable toxins present in nature, or a chemical food poisoning that causes symptoms by artificial chemicals. It can be divided into Among them, bacterial food poisoning is caused by various non-invasive pathogens such as Staphylococcus aureus, Bacillus cereus, Clostridium, and enterotoxin-coliogenic bacteria, which produce toxins in the intestine.
  • non-invasive pathogens such as Staphylococcus aureus, Bacillus cereus, Clostridium, and enterotoxin-coliogenic bacteria, which produce toxins in the intestine.
  • Food poisoning is prevented through the early detection of food poisoning bacteria, as the incidence rate increases due to changes in dietary patterns such as the expansion of group meals such as schools and companies, and increased eating out, and environmental changes such as global warming and rising room temperature.
  • the development of the technology is required.
  • the currently developed methods for detecting food poisoning bacteria include polymerase chain reaction using primers capable of amplifying specific genes of pathogenic microorganisms (KR 10-0671501), PCR detection kits for detecting food poisoning pathogens (KR 10-0994820), As a method for detecting food poisoning bacteria using a real-time PCR technique (KR 10-1003846), a limited detection method with limited time and space has been used as a method for detecting and analyzing in a laboratory through collected samples.
  • the present inventors produced a QR code and / or bar code that is modified by specifically detecting the LPS of Staphylococcus aureus or Gram-negative bacteria known as a representative microorganism of food poisoning among Gram-positive bacteria, foods of bacteria under food packaging application
  • a smart packaging system was built to visually identify and identify contamination. This enables the application of a variety of smart packaging technologies using sensitive and specific antigen-antibody responses by replacing only antibodies specific for various antigens.
  • Patent Document 1 Korean Registered Patent No. 10-0671501 (2007.01.12)
  • Patent Document 2 Korean Patent Registration No. 10-0994820 (2010.11.10)
  • Patent Document 3 Korean Registered Patent No. 10-1003846 (2010.12.17)
  • the present invention establishes a linkage system between an antibody indicator and a QR code and / or a barcode that can detect bacteria, and detects and detects bacterial contamination in a food package, rather than a limited detection method for the collected samples.
  • the present invention provides a food packaging kit for bacterial detection comprising a bacterial specific antibody indicator and a QR code and / or a barcode.
  • the present invention also provides a bacterial detection test system for food.
  • the present invention provides a food wrapping paper comprising a food packaging kit for detecting the bacteria.
  • the present invention provides a food packaging kit manufacturing method.
  • the present invention also provides a method for detecting food contaminated with bacteria.
  • the present invention provides the use of a food packaging kit for use in the detection of food contaminated with bacteria.
  • Consumers can identify whether the food is contaminated by the bacteria at the purchase site, and can be easily and quickly detected by the food inspection system according to the present invention even in a mass distribution stage.
  • FIG. 1 is a view showing a method of manufacturing a food packaging kit according to the present invention.
  • Figure 2 is a photograph showing the modification of the cord when the food packaging kit according to the present invention is exposed to SEB of Staphylococcus aureus.
  • Example 3 is a graph showing the total bacterial count of Staphylococcus aureus collected from contaminated food according to Example 1.
  • Example 4 is a graph showing the remaining amount of SEB of contaminated food according to Example 2.
  • Figure 5 is a graph showing the total bacteria and growth curve of E. coli according to Example 4.
  • Figure 6 is a photograph showing the color change by the Gram-negative bacteria of the Gram-negative bacteria specific antibody indicator membrane according to the present invention.
  • Figure 7 is a photograph showing the modification of the code when the food packaging kit according to the present invention is exposed to Gram-negative bacteria E. coli .
  • the present invention provides a food packaging kit for bacterial detection comprising a bacterial specific antibody indicator and a QR code and / or a barcode.
  • the bacteria are preferably Gram-positive bacteria or Gram-negative bacteria, and more preferably food poisoning bacteria, but are not limited thereto.
  • the antibody indicator is preferably, but not limited to, an exotoxin specific antibody indicator of Gram-positive bacteria or an LPS-specific antibody indicator of Gram-negative bacteria.
  • the exotoxin is preferably enterotoxin, more preferably SEB of Staphylococcus aureus, but is not limited thereto.
  • the QR code and / or bar code is preferably a color modification is induced by the antigen detected through the antibody indicator, but is not limited thereto.
  • the antibody specific to the SEB of Staphylococcus aureus or LPS of Gram-negative bacteria is not limited thereto.
  • the "QR code and / or bar code” means “QR code and bar code together” and “QR code or bar code each independently”.
  • antibody indicator refers to a site where a flow-type and fixed membrane containing bacterial specific antibodies and microspheres changes color by antigen-antibody reactions by bacterial antigens.
  • QR code used in the present invention refers to a code in a two-dimensional (matrix) format that contains various information in a square horizontal and horizontal grid.
  • bar code refers to a character or number coded by combining black bars and white bars having different thicknesses in order to make the computer easier to read information.
  • the inventors applied QR codes and / or barcodes to antibody indicators capable of detecting bacteria and applied them to food packaging.
  • the food packaging kit according to the present invention detects Gram-positive toxins or Gram-negative bacteria by the antibody indicator in the package, and transfers the detected signals to QR codes and / or barcodes, before the detection of the membrane to which the antibody is immobilized.
  • the color appears on the QR code and / or barcode, but after detection it causes deformation due to discoloration of the cord by latex microspheres mixed on the membrane.
  • the color can be selected according to the latex microsphere, and the code can be modified according to the size or shape of the detection area and the contact area of the code.
  • Such code deformation can be directly checked by the naked eye or detected by the code reader. Can be confirmed by a notification sound or an alarm message (see FIG. 2).
  • the antibody indicator detects bacteria by two antibodies including a flow-type membrane to which polyclonal antibodies are immobilized and a fixed membrane to which monoclonal antibodies are immobilized, as shown in FIG. 1.
  • Antibodies can detect a variety of bacteria through the replacement of antibodies on the same system, and monoclonal and polyclonal antibodies act in combination to increase the diversity and sensitivity of the detected bacteria.
  • the sample is absorbed by the blotter and then moved to the membrane.
  • Adsorbents are located at both ends of the kit, so that the sample moves due to capillary action of the sorbent, and the absorption force is determined by the characteristics of the membrane.
  • the flowable membrane is a site where the microspare attached polyclonal antibody can flow, and the antigen of the sample is detected by the polyclonal antibody and moved to the fixed membrane.
  • the flow-type membrane has a form that is placed on top of the antibody, not fixed.
  • the immobilized membrane is attached to a monoclonal antibody and modified by a substance detected in the flow antibody to transmit a signal to a QR code and / or a barcode.
  • the signal shows a visually identifiable color of the microsphere attached to the floating polyclonal antibody, and when detected by the immobilized antibody, the signal is fixed in the order of monoclonal antibody-antigen-polyclonal antibody-microsphere from below. It is a principle of color.
  • Enterotoxins of Staphylococcus are divided into 5 types: A, B, C, D and E.
  • SEB Staphylococcal Enterotoxin B
  • type B among the enterotoxins produced by Staphylococcus aureus.
  • E. coli Escherichia coli bacteria belonging to the group (hereinafter E. coli) is a Gram-negative bacteria
  • Vero toxin most common bacteria that produce toxins also known as Shiga toxin
  • E. coli O157: H7 Abbreviated as E. coli O157 or O157
  • E. coli infections that are commonly reported or colonized in the news are usually due to E. coli O157: H7.
  • the mortality rate of E. coli O157: H7 is as low as 6-7 per 1000, but it is highly contagious and spreads in a short time.
  • Verotoxins exhibit potent cytotoxicity and are the causative toxins of febrile colitis, hemolytic uremic syndrome, and encephalopathy during intestinal hemorrhagic E. coli infections.
  • the present invention also provides a food wrapping paper comprising a food packaging kit for bacteria detection.
  • the packaging paper is polyvinyl chloride (PVC: Polyvinyl Chloride), polyester (PET: Polyester), polyethylene (PE: Polyethylene), polypropylene (PP: Polypropylene), polyamide (polyamide), ethylene vinyl alcohol (harmless to humans)
  • EVOH Ethylene-vinyl alcohol
  • Ethylene-vinyl acetate EVA
  • PVDC Polyvinylidenechloride
  • PS Polystyrene
  • PET Polyethylene terephthalate
  • PET Polyethylene terephthalate
  • Nylon NY
  • PC Polycarbonates
  • PC Polycarbonates
  • acetyl cellulose Acetyl Cellulose
  • acetylbutyl cellulose Acetyl Butyl Cellulose
  • plastic composite film and modified biodegradable film of any one material is preferably used, but is not limited thereto. It doesn't work.
  • the film paper may be made of any one of transparent, translucent and opaque film paper, and such film paper may be made of any one of various colors.
  • It provides a food packaging kit manufacturing method comprising the QR code and / or the transparent modification induction region of the bar code, and the antibody indicator is placed in an overlapping position on the film of the antibody indicator.
  • the coating is preferably coated by compressing the film on the portion except for the detection start point and the discharge point, but is not limited thereto.
  • the polyclonal antibody, latex microspheres, and buffer solution (pH 7.4) of the flow-type membrane have a final concentration of polyclonal antibody in a ratio of 1: 8: 71 or 1% latex microspheres in which 10% latex microspheres are diluted with a buffer solution.
  • the mixing is preferably 12.5 / ml, but is not limited thereto.
  • the transparent modification induction region of the QR code and / or barcode of step 4) coincides with the color modified portion of the antibody indicator color developed by the antibody when contamination is detected, and a blank portion between the QR code and / or barcode code through color development.
  • the present invention provides the use of a food packaging kit for use in the detection of food contaminated with bacteria.
  • the inventors produced a food packaging kit in which an antibody indicator for detecting Gram-negative bacteria and a QR code and / or a barcode were combined.
  • colloidal gold was bound to an anti-LPS polyclonal antibody.
  • Gold colloid was adjusted to pH 5.5 (stock), pH 6.2, pH 7.2, pH 9.2 and pH 11.2 with 0.1 MK 2 CO 3 , and 200 ⁇ l each was dispensed.
  • Antibodies were added to each gold colloid in sequential concentrations from 350 ng to 750 ng in 50 ng increments.
  • the gold-binding antibody solution was dispensed 15 ⁇ l per cm onto the GF membrane and dried at 37 ° C. for 1 hour and then stored at 4 ° C. to prepare a conjugated pad.
  • LPS polyclonal and anti-mouse antibodies were diluted in pH 9.6 carbonate coating buffer and glycerol was added, followed by 1 ⁇ l test line and control line.
  • the gram-negative bacterial specific antibody indicator was assembled by attaching the sample pad, the binding pad, the membrane, and the adsorption pad in the order from the left side.
  • the detection point of the antibody indicator and the transparent modification induction point of the QR code were placed on the acetate film.
  • a bar code and a QR code were attached on the transparent film by overlapping a portion of the color development through detection and a barcode-inducing region of the QR code by detecting an gram negative bacterium LPS.
  • the starting point of the Gram-negative bacteria integrated detection antibody indicator was in direct contact with meat, and the remaining indicator area was placed on the wrapping paper.
  • a barcode that specifically detects Gram-negative bacteria that the detection identification region of the antibody indicator is white as the color of the membrane when not detected, and is changed to red (selectable according to the color of the colored latex bead) when the contamination is detected.
  • a QR code associated food packaging kit was produced (FIG. 1).
  • the present inventors replaced the antibody in the antibody indicator of Example ⁇ 1-1> with an antibody indicator for detecting enterotoxin in exotoxins secreted by Gram-positive bacteria and a food packaging kit in which QR code and / or barcode were combined. Produced.
  • a monoclonal antibody specific to SEB an enterotoxin of Staphylococcus aureus, a representative Gram-positive bacterium, latex microspheres (Bangs lab.) Showing red color, and sodium phosphate buffer were mixed in a fluidized membrane. Thereafter, polyclonal antibodies specific for SEB were attached by hydrophobic binding to the Millipore Hi-flow membrane for immobilized antibody site fabrication. Both membranes were fixed to each other and sorbent paper 3M paper was attached to both ends. Except for the detection start point and the discharge site sorbent paper, antibody indicators were prepared by pressing with acetate film.
  • a gram-positive bacteria specific food packaging kit was produced in which the detection identification region of the antibody indicator was white as the color of the membrane when not detected, and transformed into red (selectable according to the color of the colored latex bead) when contamination was detected. (FIG. 1).
  • the present inventors have prepared a standard curve of Staphylococcus aureus bacteria in order to use a Gram-positive bacteria specific food packaging kit to select an applicable food group.
  • Staphylococcus aureus was cultured in tryticase soy broth (TSB) (purchased from BD company) in a 37 ° C cell incubator. It was. The cultured Staphylococcus aureus was diluted to decimal and spread on Baird-Parker agar (BPA) (BD company) in a selective medium with egg yolk, incubated for 24 hours at 37 °C cell incubator and colony count analysis (colony) Colony forming unit (CFU) was measured by counting assay.
  • TTB tryticase soy broth
  • BPA Baird-Parker agar
  • CFU colony count analysis
  • the extracted chromosomal DNA was each 5 pmol of F_primer (5'-ATTTAACAACTCGCCTTATGAAAC-3 ') and R_primer (5'-TTAGATTGGTCAAATTTATCTCCTG-3').
  • F_primer 5'-ATTTAACAACTCGCCTTATGAAAC-3 '
  • R_primer 5'-TTAGATTGGTCAAATTTATCTCCTG-3'
  • SYBR FAST qPCR Master Mix A standard curve was prepared by comparing and analyzing the CFU value measured by colony coefficient analysis with the threshold cycle (Ct) value measured by real-time PCR.
  • the present inventors selected a food group applicable to the Gram-positive bacteria specific food packaging kit.
  • the initial denaturation temperature was 10 minutes at 95 °C, 55 cycles were performed at 95 °C 10 seconds, 55 °C 10 seconds, 72 °C 10 seconds. Then, the total number of staphylococcus aureus bacteria in contaminated food was determined by comparative analysis with the standard curve prepared in Experimental Example ⁇ 1-1>.
  • the present inventors prepared a standard curve of Staphylococcal Exotoxin B (SEB) of Staphylococcus aureus to measure the amount of exotoxins that cause food poisoning in foods contaminated with representative Staphylococcus aureus among Gram-positive bacteria.
  • SEB Staphylococcal Exotoxin B
  • SEB was diluted to 100 ng / mL, 200 ng / mL, 500 ng / mL, 1 ⁇ g / mL, 2 ⁇ g / mL, 5 ⁇ g / mL, and 10 ⁇ g / mL, respectively, into each well of the Microtiter plate.
  • Anti SEB monoclonal antibody diluted to 5 ⁇ g / mL in carbonate coating buffer was added and reacted for 2 hours at 37 °C and washed twice with wash solution (0.05% tween-20 in PBS). Then, the cells were reacted at 37 ° C. for 2 hours at 37 ° C.
  • the present inventors selected a food group applicable to a food packaging kit for detecting exotoxins of Gram-positive bacteria.
  • beef, pork, apple, milk, and orange juice were selected as five representative agricultural foods, and 5 g each of beef, pork and apple, and 5 mL of milk and orange juice were obtained and contaminated with Staphylococcus aureus.
  • the contaminated agri-food was diluted in 45 mL of peptone water and mixed vigorously to collect all Staphylococcus aureus attached to the food surface.
  • Staphylococcus aureus collected from food was boiled for 10 minutes at 100 °C for ELISA analysis, and then centrifuged at 15000 rpm for 10 minutes to obtain a protein.
  • the ELISA technique for measuring the residual amount of SEB in contaminated food proceeded in the same way as the standard curve was prepared, and the antigen was changed to a sample collected from contaminated food. Immediately after staining with Staphylococcus aureus, the amount of SEB remaining in food and uncontaminated food (control) after 1 day, 2 days and 3 days was quantitatively measured.
  • the present inventors confirmed the exotoxin detection of Gram-positive bacteria in meat packaged with the Gram-positive bacteria-specific food packaging kit prepared in ⁇ Example 2>.
  • Example 2 the detection point of the Gram-positive bacteria-specific food packaging kit prepared in Example 2 was superimposed on meat contaminated with Staphylococcus aureus, and packaged similarly to commercially available meat.
  • the present inventors confirmed the total bacterial count and growth curve of the Gram-negative bacteria E. coli to check the applicability of the Gram-negative bacteria-specific antibody indicator.
  • 0.1 mL of the dilution was taken in a petri dish and pour plating using MacKonkey agar, an E. coli selection medium. The medium was hardened at room temperature and then incubated at 37 ° C. for 24 to 48 hours. After cultivation of bacteria, the colonies were counted to obtain a log value, and then a growth curve was calculated.
  • the inventors of the present invention confirmed the Gram-negative bacteria-specific detection and a change in the code due to the Gram-negative bacteria-specific food packaging kit prepared in Example 1.
  • control medium LB
  • PBS B. cereus in the gram-positive bacteria of the negative control
  • E. coli in the gram-negative bacteria of the Gram-positive bacteria specific food packaging kit prepared in Example 1
  • the specificity of the antibody clock against the Gram-negative bacteria was confirmed by one minute after confirming that the membrane specific to E. coli , a Gram-negative bacterium, changed to red (Fig. 6).

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Abstract

Cette invention concerne un kit d'emballage alimentaire comprenant un indicateur d'anticorps spécifiques de bactéries, un code QR et/ou un code-barres, et une technique permettant d'identifier sur site la contamination d'une denrée alimentaire par des bactéries à l'aide du kit d'emballage alimentaire. Le kit d'emballage alimentaire selon l'invention permet à un consommateur d'identifier sur le lieu d'achat si une denrée alimentaire distribuée est contaminée ou non par des bactéries qui provoquent des intoxications alimentaires. En plus, la contamination alimentaire par des bactéries qui provoquent des intoxications alimentaires peut être simplement et rapidement détectée même lors d'un procédé de distribution de masse de denrées alimentaires à l'aide d'un système d'analyse alimentaire selon la présente invention.
PCT/KR2012/010799 2012-02-03 2012-12-12 Kit d'emballage alimentaire permettant de détecter des bactéries WO2013115478A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20120011250 2012-02-03
KR10-2012-0011250 2012-02-03
KR10-2012-0144496 2012-12-12
KR20120144496A KR101494502B1 (ko) 2012-02-03 2012-12-12 세균 검출용 식품 포장 키트

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108414747A (zh) * 2017-02-10 2018-08-17 国家纳米科学中心 条码化层析试纸条的制备方法、其检测装置及应用
WO2019082145A1 (fr) * 2017-10-27 2019-05-02 Tubitak Bandelette réactive d'immunochromatographie

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0306336A2 (fr) * 1987-09-04 1989-03-08 Syntex (U.S.A.) Inc. Dispositif d'essai à multiples orifices
EP0587222A2 (fr) * 1992-08-31 1994-03-16 Johnson & Johnson Clinical Diagnostics, Inc. Eléments secs avec une couche absorbante séparée pour immunoessais
WO1994027144A1 (fr) * 1993-05-19 1994-11-24 California South Pacific Investors Detection de contaminants dans les aliments
WO1999014598A1 (fr) * 1997-09-15 1999-03-25 California South Pacific Investors Systeme de detection pour aliments

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0306336A2 (fr) * 1987-09-04 1989-03-08 Syntex (U.S.A.) Inc. Dispositif d'essai à multiples orifices
EP0587222A2 (fr) * 1992-08-31 1994-03-16 Johnson & Johnson Clinical Diagnostics, Inc. Eléments secs avec une couche absorbante séparée pour immunoessais
WO1994027144A1 (fr) * 1993-05-19 1994-11-24 California South Pacific Investors Detection de contaminants dans les aliments
WO1999014598A1 (fr) * 1997-09-15 1999-03-25 California South Pacific Investors Systeme de detection pour aliments

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108414747A (zh) * 2017-02-10 2018-08-17 国家纳米科学中心 条码化层析试纸条的制备方法、其检测装置及应用
CN108414747B (zh) * 2017-02-10 2023-03-31 国家纳米科学中心 条码化层析试纸条的制备方法、其检测装置及应用
WO2019082145A1 (fr) * 2017-10-27 2019-05-02 Tubitak Bandelette réactive d'immunochromatographie

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