WO1999013316A1 - Systeme d'examen des urines - Google Patents

Systeme d'examen des urines Download PDF

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Publication number
WO1999013316A1
WO1999013316A1 PCT/JP1997/003156 JP9703156W WO9913316A1 WO 1999013316 A1 WO1999013316 A1 WO 1999013316A1 JP 9703156 W JP9703156 W JP 9703156W WO 9913316 A1 WO9913316 A1 WO 9913316A1
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WO
WIPO (PCT)
Prior art keywords
image
particle
classified
classification
displayed
Prior art date
Application number
PCT/JP1997/003156
Other languages
English (en)
Japanese (ja)
Inventor
Kazuhisa Machida
Takafumi Kawasaki
Kaori Kina
Yasuaki Kojima
Kazuhiro Sano
Original Assignee
Hitachi, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi, Ltd. filed Critical Hitachi, Ltd.
Priority to PCT/JP1997/003156 priority Critical patent/WO1999013316A1/fr
Publication of WO1999013316A1 publication Critical patent/WO1999013316A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/493—Physical analysis of biological material of liquid biological material urine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/20—Measuring for diagnostic purposes; Identification of persons for measuring urological functions restricted to the evaluation of the urinary system

Definitions

  • the present invention relates to a urine test system suitable for performing image processing and re-classification of sediment components contained in urine and highly accurate classification of sediment components on a screen.
  • Particles in urine such as various sediment components such as blood cells and tissue cells, are closely related to human health. Analyzing this sediment component is one of the important test items in conducting clinical tests.
  • urine is first centrifuged to separate urine and sediment components. Separated sediment components were stained if necessary, specimens were prepared on slide glass, and laboratory technicians performed inspection and classification while observing with a microscope.
  • Urine sediment components are classified according to their characteristics such as shape and stainability.However, even the same component shows a wide variety of shapes, making classification very difficult.Therefore, only sediment components having a typical shape are used. Other sediment components that are classified correctly and are difficult to classify can be misclassified.To solve such a problem, in recent years, the classification of particles in urine has been automatically performed. An automatic analyzer and an automatic analysis method have been issued. C Among the above-described automatic analyzers and automatic classification methods, an example of performing image processing of particles on a screen is disclosed in JP-A-8-293. No. 17 Gazette, Special
  • the technology described in Japanese Patent Application Laid-Open No. Hei 9-1991 is disclosed.
  • the items to be re-sorted are set in advance on the re-sorting item setting screen, and then the setting is made by switching from the re-sorting item setting screen to the particle image display screen.
  • the particle image of the reclassified item By displaying the particle image of the reclassified item on the screen, touching the particle image to be reclassified, and touching the classification item provided at the bottom of the particle image display screen, the particle can be reclassified.
  • Japanese Patent Application Laid-Open No. 9-191430 discloses that particle images that have been automatically classified are recognized, and only the particle images and the recognition results with high recognition reliability are stored in a storage device. In response, the particle image and the recognition result are displayed on the screen, and if the displayed recognition result is incorrect, the operator corrects the particle recognition by using the keyboard while looking at the displayed particle image. It describes reclassification and storing the reclassification result in a storage device.
  • An object of the present invention is to provide a urine test system having a particle image classification method that can efficiently perform particle image processing and particle classification. Disclosure of the invention
  • the present invention provides a urine test system for processing an image of particles contained in urine on a display screen, wherein a plurality of windows are displayed on the display screen, By processing the particle images between the plurality of windows, re-classification of the particle images and classification of the particle images with higher accuracy are performed.
  • a particle image automatic classification device that automatically classifies images of particles contained in urine and a particle image classification unit that reclassifies the classified particle images on a display screen are provided. Equipped urine test system,
  • a plurality of windows are displayed on the display screen, and the particles are reclassified among the plurality of windows by the reclassification method including the following steps.
  • the particles are re-classified among a plurality of windows displayed on the display screen by the re-classification method including the following steps.
  • the particles can be re-classified without switching the display screen, so that a urinalysis system with improved operability can be provided.
  • FIG. 1 is a configuration diagram of a urine test system according to an embodiment of the present invention
  • FIGS. 2 to 10 are diagrams showing an embodiment of the present invention
  • FIGS. 11 and 12 are diagrams showing a second embodiment of the present invention.
  • FIGS. 13 and 14 are diagrams showing a third embodiment of the present invention.
  • FIG. 16 is a diagram showing a fourth embodiment of the present invention, and
  • FIG. 17 is a diagram showing a fifth embodiment of the present invention.
  • FIG. 1 is a diagram showing a configuration of a urine test system according to one embodiment of the present invention.
  • the urine test system consists of an automatic particle image classifier 1 that automatically classifies particle images, and a particle image classifier that manually reclassifies automatically classified particle images and classifies particle images that were not automatically classified. It comprises a device 2 and a server 12, and the particle image automatic classification device 1, the particle image automatic classification device 2, and the server 1 12 are connected by a bus 9.
  • the particle image automatic classification device 1 is composed of a flow cell 3, a flash lamp 4, a CCD camera 5, an image processing unit 6, an image memory 7, and a lamp control unit 8. , A data storage unit 10 and an image display unit 11.
  • the image display section 11 includes a display 13, a main body 14 having a CPU, a mouse 15, and a keyboard 16.
  • the display 13 uses a CRT display, a liquid crystal display, a plasma display, or the like.
  • the urine sample flowing through the flow cell 3 is irradiated with the pulse light from the flash lamp 4 and converted into an image signal by the CCD camera 5.
  • the image processing unit 6 detects features such as particle surface and jil Pil J average density from the image signal, and classifies the particle surface image.
  • Classified particles are stored in the image memory 7 and, after the classification of the particle images is completed, transferred to the server 12 via the bus 9 and stored in the server 12.
  • the specific configurations and operations of the particle image automatic classifying device 1 and the particle image classifying device 2 are described in Japanese Patent Application Laid-Open No. Hei 8-29137.
  • information on the specimen such as a specimen list and qualitative data is also stored.
  • FIG. 2 is a flowchart showing an embodiment of the present invention.
  • the sample data is integrated into the inspection data display section 302, the qualitative data display section 303, and the classification result display section 304 of the standard screen 300 as shown in FIG.
  • This is a flow chart for performing an operation for indicating and an operation for indicating a particle image for reclassifying the self-K / classified particle-m image in the work area 308.
  • the standard screen 300 is automatically displayed (step 202).
  • the standard screen 300 includes a large classification item icon 301 indicating the classification of particles contained in a sample such as urine, and a sample data display section 3 for displaying sample information.
  • the toolbar 305 includes a sample list button 401, a next sample button 402, a review complete button 403, and a sample image display button 404. It is composed of 04, a sample data edit button 405, and a sample list display button 406.
  • the specimen data display section 302 In the standard screen 300 in step 202, the specimen data display section 302, the qualitative data display section 303, the classification result display section 304, and the work error 308 are blank.
  • step 203 in the above-mentioned state, the sample has not yet been specified, and the sample data display section 302 and the qualitative data display section 303 constituting the standard screen 300 have been classified. Since information such as data and images is not displayed on the result display section 304 and the work error area 108, the operator displays a list of examinations to perform work by designating a sample. Select a sample from the list (step 204).
  • the abduction image classifier itt The display of the sample list and the next sample, which are the basic processes of 2, the completion of the review to finish the particle image classification process, the display of the standard particle image, and the designation of the mode of addition, change, and deletion of sample data
  • the contents for editing the sample data, displaying the sample name, etc. in a batch are displayed.
  • This display is arranged in a line at the top of the screen, as shown in toolbar 350. With such an arrangement, it is possible to view the entire basic processing functions, and it is easy to select the functions to be processed.
  • step 204 the sample list is displayed from the toolbar 305. Select the sample list button 401 to display the sample list. By specifying the sample list button 401 with the mouse 15 and clicking, the sample list button is displayed as shown in Fig. 5.
  • the list screen 500 displaying the list is displayed on the standard screen 300. (Step 205) The numerical data displayed on the list screen 500 shown in FIG. It is described to explain the examples, and actually relates to each sample. Numerical data is displayed.
  • Step 206 From the displayed list screen 500, select an examination for processing, for example, a sample 501 for classifying particles (step 206), and list the list 50
  • the inspection data is displayed on the specimen data display section 302, qualitative data display section 303, and classification result display section 304 displayed on the standard image 300.
  • the data displayed on the qualitative data display section 303 is such that when a sample is selected, data relating to the selected sample is sent to the server 12 from another analyzer or a host computer not shown in FIG. Displayed via server 1 1 2.
  • FIG. 6 shows an example of the fine classification items displayed on the classification result display section 304.
  • 601 indicates an item number
  • 602 indicates an item code
  • 603 indicates a classification item
  • 604 indicates an abbreviation of the classification item displayed on the classification result display section 304.
  • the sample data display section 302, the qualitative data display section 303, and the classification result display section 304 are arranged in a vertical line, and the work area 310 Since it is displayed at the same time, there is an effect that the screen is easy to see and use.
  • step 208 After displaying the sample data, the process returns to step 203 again and proceeds to step 208.
  • step 208 one of the icons of the particles to be re-classified is selected from the large classification item icons 301.
  • the red blood cell icon 301a is selected will be described with reference to FIG.
  • erythrocyte icon 301a When the erythrocyte icon 301a is clicked with the mouse 15, a list 701 of erythrocyte sub-categories as shown in FIG. 7 is displayed (step 209). As shown in Fig. 3, the major category item icons 301 are arranged in a row in a row according to the question of the tool bar 310 and the work area 308. By clicking on the large classification item icon 301 with the mouse 15, the detailed classification item is displayed, which is effective in that the classification operation can be easily performed.
  • the item 7 0 2 (for example, other epithelium) to be re-classified is clicked with the mouse 15 (step 2 10).
  • the automatically classified particle images as shown in the figure are displayed in the image window 801 (step 211).
  • the particle image displayed in the image window 801 is schematically described for explaining the embodiment, and a particle image having various shapes and colors is actually displayed.
  • the image window 8001 is composed of a vertical ruler 802 that indicates the reference dimension of the particle image in the vertical direction, and a horizontal ruler 803 that indicates the reference dimension of the particle image in the horizontal direction. And is displayed in the work area 308 on the standard screen 300. Then, by operating the mouse 15 and the keyboard 16, the particles are re-classified on the work area 310.
  • FIG. 9 a specific method of reclassifying particles will be described with reference to FIGS. 9 and 10.
  • FIG. 9 is a flowchart showing an operation from step 211 to step 212 of the flowchart shown in FIG.
  • the operation in the case of using the red blood cell image window will be described.
  • a frame 1002 with a color such as light blue is attached to the selected particle surface image 1001 (step 902).
  • step 903 the operator visually checks the presence of the icon of the particle to be classified into the particle image 1001 selected on the large classification item icon 301.
  • the large category item icons 301 are arranged in a horizontal line between the toolbar 305 and the work area 308 so that the operator can easily see them. This has the effect of making it easier to find icons for which particle images should be classified.
  • Step 904 If there is an icon of a particle to which the particle image 1001 is to be classified on the large classification item icon 301, drag the particle image 1001 with the mouse 15 (step 904), and The particles are re-classified by dropping on the icon of the particles to be re-classified on the classification item icon 301 (step 905). (Step 906) o
  • a shadow 1003 is added to the reclassified particle image 1001, so that it is possible to recognize that the particle has moved by the reclassification. After that, the process returns to step 901 to reclassify the particles remaining in the image window 801.
  • step 903 if the selected particle image 1001 does not exist on the large category item icon 301, proceed to step 907, and right-click the mouse 15 on the particle image 1001. Click the button. By clicking the right button of the mouse 15, a menu image is displayed (step 908).
  • “sub-classification item” is selected from the menu screen (step 909), As shown in Fig. m10, the standard of subclassification of particles ⁇ ⁇ ⁇ 1004 and the candidate of the next subclassification 3 ⁇ 4 1005 are standard I ⁇ ⁇ 3 0 0 It is displayed on the rear 308 (step 910).
  • step 9 12 If the displayed items 1 1 0 4 and 1 0 5 are not items for which the particle image 1 0 1 should be reclassified, click the right button of the mouse 15 again to sequentially select candidates. Is displayed. Then, when the sub-category to which the particle image 1001 is to be classified is displayed, the corresponding sub-category is clicked with the mouse 15 (step 911), whereby the particle image is displayed. Reclassification is performed (step 9 12).
  • the particle image 1001 is dragged with the mouse 15 (step 912), and dropped on the selected reclassification item (step 913), thereby re-establishing the particle. Classification is performed (step 914).
  • the particle image can be promptly reproduced.
  • the effect is that classification can be performed.
  • a shadow 1003 is added to the reclassified particle image 1001, so that it is possible to recognize that the particle has moved by the reclassification. After that, the process returns to step 901 to reclassify the particles remaining in the image window 801.
  • red blood cell icon 301 a is clicked again with the mouse 15 to list the subclasses of red blood cells 7 0 Remind one.
  • the configuration of the standard screen 300 of the urine test system is that the image window of the particle image to be re-classified next without closing the image window 800 1 Dows can be opened.
  • particles can be re-classified on one screen, so that it is not necessary to switch the screen for each particle to be re-classified, and good operability in which each processing operation can be performed with a mouse.
  • a simple urinalysis system can be provided.
  • the present embodiment the case where the particle images are re-classified has been described.
  • the present embodiment is also applied to the case where the classified particle images are to be classified with higher dermis. It is possible to do so.
  • the urine test system described in this embodiment is, for example, a Windows system developed by Microsoft (Windows is a trademark of Microsoft). It is possible to use.
  • FIG. 11 is a flowchart showing an operation for correcting the particle image displayed in the image window and reclassifying the corrected particle image.
  • an operation using an image window of red blood cells will be described.
  • image processing is performed by double-clicking the particle image 1201 to be corrected from the image window 801 with the mouse 15. Perform (Step 111).
  • the particle image 1201 is displayed in the image processing window 122 (step 1102).
  • the particle image 1201 in the selected image window 8001 is provided with a frame 1002 colored with light blue or the like.
  • the image processing window 1 202 is used as a vertical ruler 1203 that indicates the vertical dimension of the particle image 1201 and a horizontal ruler for the particle image 1201.
  • Horizontal ruler 1 204 indicating dimensions and magnification of particle image 1 201.
  • Magnification button 1 205 to set and display reduction ratio and button to close image processing window 1 202 And a work area 308 in the standard screen 300.
  • step 1103 the particle image 1201 shown in the image processing window 1202 is corrected.
  • the correction of the particle image 12 ⁇ 1 includes edge correction to clarify the contour of the particle, gamma correction to adjust the particle color, and magnification change Hi of the particle image.
  • step 1104 the operator visually observes whether or not the re-classification is necessary for the corrected particle processing image 1201 in the image processing window 122, and re-executes the re-classification. If classification is not required, click button 1 206 with mouse 15 to close image processing window 1 202 and return to image window 8 0 1 (step 1 1 0 0 ). After returning to the image window 801, if necessary, correct other particle images displayed in the image window 801.
  • step 1104 if it is necessary to re-classify the particle image 1 201, proceed to step 1 105, and place the image processing window 1 202 on the large classification item icon 310. The operator visually checks the presence of the icon of the particle to be classified into the particle image 1 201 displayed on the screen.
  • step 1106 If there is an icon of the particle to which the particle image 1 201 is to be classified on the large classification item icon 3 0 1, the particle image 1 2 0 1 in the image processing window 1 0 2 Drag by step 5 (step 1106), and drop on the icon of the particle to be reclassified that exists on the major category icon 301 (step 1107). The reclassification is performed (step 1108).
  • the particle image 1 201 displayed in the image processing window 1 202 is clicked on the button 1 206 with the mouse 15 and the image processing window ⁇ 1 202 is displayed. Close to return to Image Window 800.
  • the particle image 1201 returned to the plane image window 8001 has a shadow 1003 added to it so that it can be recognized that the particle has moved by the ⁇ classification. Then, the process returns to step 110, and if necessary, corrects and re-classifies the particle images remaining in the image window 800.
  • step 1105 if the particle image 1 201 displayed in the image processing window 1 202 does not exist on the large item icon 310, then go to step 110 Go on and click the right button of mouse 15 on the particle image 1 201 displayed in the image processing window 1 202.
  • Clicking the right button of the mouse 15 displays the menu screen (step 1 11 10), and when you select “Sub-category item” from the menu screen (step 1 11 1), As shown in Fig. 12, the item 1207 as a candidate for sub-classification to be classified into particles and the item 1208 as a candidate for the next sub-classification item are displayed on the standard screen 300. It is displayed on the set work area 308 (Step 1 1 1 2). If the displayed items 1207 and 1208 are not items for which the particle image 1221 should be reclassified, click the right button of the mouse 15 again, and so on. The candidate sub-category items are displayed.
  • step 111 when a sub-category item to which the particle image 1 201 is to be classified is displayed, the corresponding sub-category item is selected by the mouse 15 (step 1 113).
  • step 1114 drag the particle image 1201 displayed in the image processing window 122 with the mouse 15 (step 1114), and drop it on the selected reclassification item. By doing so (step 111), the particles are reclassified (step 111).
  • the reclassified particle image 1201 can be obtained by clicking the button 1206 with the mouse 15 and then -j in the image processing window 122. Return to 801.
  • the particle image 1201 in the image window 8001 has a shadow 1003 added to it so that it can be identified as a child abducted by classification. Thereafter, the process returns to step 110, and if necessary, the image of the grain remaining on the image window 8 0 1 is obtained. Correction and re-classification.
  • the display of the particle image in the image window and the correction and re-classification of the particle image in the image processing window can be performed on one screen, so that there is no need to switch the screen.
  • a urinalysis system with good operability can be provided.
  • the first 3 figures in Step 1 3 0 1 c first Figure 3 is a flow port one Chiya one preparative showing the editing operation of the sample data, the toolbar 3 0 5 provided on the standard screen 3 0 in 0, Click the sample data edit button 405 with the mouse 15.
  • the sample data 3 ⁇ 4 screen 1401 is displayed on the standard screen 300 as shown in Fig. 14 (Step 1302).
  • the sample data knitting screen 1401 is for the sample data section 1402a to 1402i, the cancel button 144, the data edit button 144, and the review button 14 0 5.
  • the numerical data shown in the specimen data volume 1401 shown in Fig. 14 are used to explain the embodiment.
  • the numerical data to be IM1 is displayed.
  • the sample data edit screen 1441 is ready for editing. From the sample data section 1402a to 1402i, select the sample for which data editing is to be performed using the mouse 15 (step 13304), and edit the data using the keyboard 16 ( Step 1305).
  • step 1306 If data editing has not been completed in step 1306, the process returns to step 1304 to select a sample, and data editing is continued.
  • sample data can be edited on one screen, so that it is possible to provide a highly operable urine test system that does not require switching screens when editing data.
  • ⁇ 15 is a flowchart showing the operation of the ⁇ classification method for particles that have been sampled [ff].
  • step 1501 in Fig. 15 from the toolbar 3 05 on the standard screen 3 00 on which the image window 8 0 1 was displayed, click the sample image display button 4 4 4 with the mouse 15 Click By clicking the sample image display button 404 with the mouse 15, the sample image window on the standard screen 300 as shown in Fig. 16 is displayed. Is displayed (Step 1502)
  • the sample image window 1601 is composed of a vertical ruler 1602 that indicates the reference dimension of the particle image in the vertical direction, and a horizontal ruler 1604 that indicates the reference dimension of the particle image in the horizontal direction. And a particle image having a standard shape is displayed.
  • step 1503 observation is performed while comparing the particle image displayed in the image window 8001 with the particle image displayed in the sample image window 1601.
  • step 1504 the image is selected (step 1503), and the particles are reclassified by the above-mentioned method (step 1504).
  • step 1505 if the re-classification is not completed, the process returns to step 1504, and the re-classification is performed. If the re-classification is completed, the image window 81 and the sample image are displayed. Close the image window 1601 and return to the standard screen 300.
  • step 1503 if there is no particle image to be re-classified, the particle image displayed in the image window 800 and the particle image displayed in the sample image window 1601 continue.
  • Step 1506 By comparing and observing the statue of the abductee (Step 1506), it is determined whether the examination is normal or abnormal (Step 1507).
  • the image window 8000 and the sample image window 1601 are added to return to the screen; According to the present invention, since a plurality of image windows can be opened on one screen, it is not necessary to switch screens when re-classifying particle images and determining whether a sample is normal or abnormal. An urinalysis system with good operability can be provided.
  • FIG. 17 is a flowchart showing an operation for temporarily storing a particle image that cannot be classified during the re-classification of the particle image.
  • the particles are re-classified by the above-described re-classification method.
  • step 1704 if it becomes impossible to classify the particle image during the re-classification, the particle image is dragged with the mouse 15 (step 1705), and the standard screen 300 is displayed.
  • step 1706 By dropping the particle image on the clipboard 307 provided above (step 1706), the particle image is temporarily stored in the clipboard 307. (Step 177).
  • the clipboard 307 has a structure that can store data for each sample.
  • the clipboard 307 is arranged K beside the major category item icon 301, it has an effect that the operator can easily find it.
  • step 1708 after the re-classification of the particle image is completed, the clipboard 3107 is clicked with the mouse 15 to reduce the clipboard 3107 to ⁇ .
  • step 1710 if there is a ⁇ classifiable particle on clipboard 307, Perform classification (step 1711), and if reclassification is not possible, return to step 1707, close clipboard 307, and transfer data to clipboard 307. save.
  • particle data can be temporarily stored during reclassification, and a reclassification operation can be performed on one screen, so that operability for efficient reclassification can be improved.
  • a urine test system can be provided.
  • the present embodiment the case where the particle images are re-classified has been described.However, the present embodiment can be applied to the case where the automatically classified particle images are classified with higher accuracy. It is possible. Industrial applicability
  • the present invention is suitable for a urinalysis system for performing classification and image processing of sediment components contained in urine.

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Abstract

L'invention concerne un système d'examen des urines capable de traiter les images de particules contenues dans les urines sur l'écran d'affichage, d'afficher une pluralité de fenêtres sur l'écran d'affichage et de reclasser les images de particules entre les fenêtres en utilisant un procédé déterminé.
PCT/JP1997/003156 1997-09-08 1997-09-08 Systeme d'examen des urines WO1999013316A1 (fr)

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PCT/JP1997/003156 WO1999013316A1 (fr) 1997-09-08 1997-09-08 Systeme d'examen des urines

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PCT/JP1997/003156 WO1999013316A1 (fr) 1997-09-08 1997-09-08 Systeme d'examen des urines

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WO1999013316A1 true WO1999013316A1 (fr) 1999-03-18

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