WO1999005283A3 - Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation - Google Patents

Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation Download PDF

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Publication number
WO1999005283A3
WO1999005283A3 PCT/FR1998/001593 FR9801593W WO9905283A3 WO 1999005283 A3 WO1999005283 A3 WO 1999005283A3 FR 9801593 W FR9801593 W FR 9801593W WO 9905283 A3 WO9905283 A3 WO 9905283A3
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WO
WIPO (PCT)
Prior art keywords
biosynthesis genes
desoxy
hexoses
transfer
saccharopolyspora erythraea
Prior art date
Application number
PCT/FR1998/001593
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English (en)
Other versions
WO1999005283A2 (fr
Inventor
Claude Fromentin
Jean-Marc Michel
Marie-Cecile Raynal
Khadidja Salah-Bey
Jesus Cortes
Sabine Gaisser
Peter LEADLAY
Carmen Mendez
Jose A Salas
Original Assignee
Hoechst Marion Roussel Inc
Claude Fromentin
Michel Jean Marc
Raynal Marie Cecile
Salah Bey Khadidja
Jesus Cortes
Sabine Gaisser
Peter LEADLAY
Carmen Mendez
Jose A Salas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR9709458A external-priority patent/FR2766496B1/fr
Priority claimed from FR9807411A external-priority patent/FR2786200A1/fr
Application filed by Hoechst Marion Roussel Inc, Claude Fromentin, Michel Jean Marc, Raynal Marie Cecile, Salah Bey Khadidja, Jesus Cortes, Sabine Gaisser, Peter LEADLAY, Carmen Mendez, Jose A Salas filed Critical Hoechst Marion Roussel Inc
Priority to EP98940290A priority Critical patent/EP1032679A2/fr
Priority to JP2000504257A priority patent/JP2001511349A/ja
Publication of WO1999005283A2 publication Critical patent/WO1999005283A2/fr
Publication of WO1999005283A3 publication Critical patent/WO1999005283A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention a pour objet la séquence d'ADN isolée représentée à la figure 2 (SEQ ID No. 1) correspondant à la région eryG-eryAIII du cluster de gènes de la biosynthèse de l'érythromycine et la séquence d'ADN isolée représentée à la figure 3 (SEQ ID No. 6) correspondant à la région eryAI-eryK du cluster de gènes de la biosynthèse de l'érythromycine, et a pour objet la séquence d'ADN isolée représentée à la figure 22 (séquence de SEQ ID No. 15) correspondant à une région du cluster de gènes de la biosynthèse de l'oléandomycine (séquence de SEQ ID No. 15).
PCT/FR1998/001593 1997-07-25 1998-07-21 Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation WO1999005283A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP98940290A EP1032679A2 (fr) 1997-07-25 1998-07-21 Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation
JP2000504257A JP2001511349A (ja) 1997-07-25 1998-07-21 Ｓａｃｃｈａｒｏｐｏｌｙｓｐｏｒａｅｒｙｔｈｒａｅａ及びＳｔｒｅｐｔｏｍｙｃｅｓａｎｔｉｂｉｏｔｉｃｕｓにおける６−デオキシヘキソースの生合成及び移送のための遺伝子

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR9709458A FR2766496B1 (fr) 1997-07-25 1997-07-25 Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et leur utilisation
FR97/09458 1997-07-25
FR9807411A FR2786200A1 (fr) 1998-06-12 1998-06-12 Genes de biosynthese et de transfert des 6-desoxy-hexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation
FR98/07411 1998-06-12

Publications (2)

Publication Number Publication Date
WO1999005283A2 WO1999005283A2 (fr) 1999-02-04
WO1999005283A3 true WO1999005283A3 (fr) 1999-05-27

Family

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Application Number Title Priority Date Filing Date
PCT/FR1998/001593 WO1999005283A2 (fr) 1997-07-25 1998-07-21 Genes de biosynthese et de transfert des 6-desoxyhexoses chez saccharopolyspora erythraea et chez streptomyces antibioticus et leur utilisation

Country Status (3)

Country Link
EP (1) EP1032679A2 (fr)
JP (1) JP2001511349A (fr)
WO (1) WO1999005283A2 (fr)

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US6399789B1 (en) 1996-12-18 2002-06-04 Kosan Biosciences, Inc. Multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides
US6388099B2 (en) 1998-10-29 2002-05-14 Kosan Biosciences, Inc. Production of 8,8a-dihydroxy-6-deoxyerythronolide B
ATE331028T1 (de) * 1999-04-16 2006-07-15 Kosan Biosciences Inc Multi-plasmid verfahren zur herstellung von grossen bibliotheken von polyketiden und nicht- ribosomalen peptiden
ES2165278B1 (es) * 1999-06-24 2003-06-16 Univ Oviedo Un procedimiento de obtencion de productos glicosilados mediante la biosintesis de los desoxiazucares: l-olivosa, l-oleandrosa y l-ramnosa.
US7033818B2 (en) 1999-10-08 2006-04-25 Kosan Biosciences, Inc. Recombinant polyketide synthase genes
US6524841B1 (en) 1999-10-08 2003-02-25 Kosan Biosciences, Inc. Recombinant megalomicin biosynthetic genes and uses thereof
DK1278881T3 (da) 2000-04-13 2010-05-10 Biotica Tech Ltd Glycosylerede hybrid-produkter og deres fremstilling og anvendelse
WO2002029035A2 (fr) * 2000-10-05 2002-04-11 Regents Of The University Of Minnesota Procede destine a modifier des fragments de sucre
FR2845394A1 (fr) * 2002-10-08 2004-04-09 Aventis Pharma Sa Polypeptides impliques dans la biosynthese des spiramycines, sequences nucleotidiques codant ces polypeptides et leurs applications
US7579167B2 (en) 2002-10-08 2009-08-25 Aventis Pharma S. Polypeptides involved in the biosynthesis of spiramycins, nucleotide sequences encoding these polypeptides and applications thereof
FR2851773A1 (fr) * 2003-02-27 2004-09-03 Aventis Pharma Sa Polypeptides impliques dans la biosynthese des spiramycines, sequences nucleotidiques codant ces polypeptides et leurs applications
AU2003300479B2 (en) * 2002-10-08 2009-12-10 Aventis Pharma S.A. Polypeptides involved in spiramycin biosynthesis, nucleotide sequences encoding said polypeptides and uses thereof
GB0327721D0 (en) * 2003-11-28 2003-12-31 Biotica Tech Ltd Polyketides and their synthesis
EP2668284B1 (fr) 2011-01-28 2014-10-15 Amyris, Inc. Criblage de micro-colonies encapsulées dans du gel
WO2012158466A1 (fr) 2011-05-13 2012-11-22 Amyris, Inc. Procédés et compositions pour détecter la production microbienne de composés immiscibles avec l'eau
BR112015002724B1 (pt) 2012-08-07 2022-02-01 Total Marketing Services Método para produzir um composto não catabólico heterólogo, e, composição de fermentação
CA2903053C (fr) 2013-03-15 2023-01-17 Amyris, Inc. Utilisation de phosphocetolase et de phosphotransacetylase pour la production de composes derives d'acetyl-coenzyme a
CN105934517A (zh) 2013-08-07 2016-09-07 阿迈瑞斯公司 用于稳定生产乙酰辅酶a衍生化合物的方法
CN107075461B (zh) 2014-11-14 2020-10-23 浙江海正药业股份有限公司 多杀菌素异源表达菌株及其构建方法和应用
AU2016284689B9 (en) 2015-06-25 2022-01-20 Amyris, Inc. Maltose dependent degrons, maltose-responsive promoters, stabilization constructs, and their use in production of non-catabolic compounds

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016334A1 (fr) * 1990-04-18 1991-10-31 Abbott Laboratories Derives d'erythromycine
WO1997006266A1 (fr) * 1995-08-03 1997-02-20 Abbott Laboratories Vecteurs et procede d'elaboration de 6,12-didesoxyerythromycine a grande purete par fermentation
WO1997023630A2 (fr) * 1995-12-21 1997-07-03 Abbott Laboratories Genes de biosynthese de sucres associes a des polyketides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016334A1 (fr) * 1990-04-18 1991-10-31 Abbott Laboratories Derives d'erythromycine
WO1997006266A1 (fr) * 1995-08-03 1997-02-20 Abbott Laboratories Vecteurs et procede d'elaboration de 6,12-didesoxyerythromycine a grande purete par fermentation
WO1997023630A2 (fr) * 1995-12-21 1997-07-03 Abbott Laboratories Genes de biosynthese de sucres associes a des polyketides

Non-Patent Citations (15)

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Title
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DONADIO S ET AL: "RECENT DEVELOPMENTS IN THE GENETICS OF ERYTHROMYCIN FORMATION", INDUSTRIAL MICROORGANISMS: BASIC AND APPLIED MOLECULAR GENETICS. ASM CONFERENCE ON THE GENETICS AND MOLECULAR BIOLOGY OF INDUSTRIAL MICROORGANISMS, 1992, pages 257 - 265, XP000607676 *
GAISSER S ET AL: "Analysis of seven genes from the eryAl-eryK region of the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea", MOLECULAR & GENERAL GENETICS, (OCT 1997) VOL. 256, NO. 3, PP. 239-251., XP002061261 *
GAISSER, S. ET AL.: "Analysis of eryBI, eryBIII and eryBVII from the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea", MOLECULAR AND GENERAL GENETICS., vol. 258, April 1998 (1998-04-01), pages 78 - 88, XP002087279 *
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QUIROS, L. & SALAS, J.: "Biosynthesis of the macrolide oleandomycin by Streptomyces antibioticus", JOURNAL OF BIOLOGICAL CHEMISTRY, (1995 AUG 4) 270 (31) 18234-9., vol. 270, 4 August 1995 (1995-08-04), pages 18234 - 18239, XP002096256 *
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STASSI D ET AL: "IDENTIFICATION OF A SACCHAROPOLYSPORA ERYTHRAEA GENE REQUIRED FOR THE FINAL HYDROXYLATION STEP IN ERYTHROMYCIN BIOSYNTHESIS", JOURNAL OF BACTERIOLOGY, vol. 175, no. 1, January 1993 (1993-01-01), pages 182 - 189, XP000608396 *
SUMMERS R G ET AL: "Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production", MICROBIOLOGY-UK, (OCT 1997) VOL. 143, PART 10, PP. 3251-3262., XP002061260 *
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Publication number Publication date
JP2001511349A (ja) 2001-08-14
EP1032679A2 (fr) 2000-09-06
WO1999005283A2 (fr) 1999-02-04

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