WO1998051778A1 - Modification des proprietes de placage de surfaces de croissance - Google Patents

Modification des proprietes de placage de surfaces de croissance Download PDF

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Publication number
WO1998051778A1
WO1998051778A1 PCT/IL1998/000213 IL9800213W WO9851778A1 WO 1998051778 A1 WO1998051778 A1 WO 1998051778A1 IL 9800213 W IL9800213 W IL 9800213W WO 9851778 A1 WO9851778 A1 WO 9851778A1
Authority
WO
WIPO (PCT)
Prior art keywords
growth
coating
coating material
coated
growth surface
Prior art date
Application number
PCT/IL1998/000213
Other languages
English (en)
Inventor
Melvyn Rosenberg Nevo
Original Assignee
Assif Science And Technology Projects Development Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Assif Science And Technology Projects Development Ltd. filed Critical Assif Science And Technology Projects Development Ltd.
Priority to AU72310/98A priority Critical patent/AU7231098A/en
Publication of WO1998051778A1 publication Critical patent/WO1998051778A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings

Definitions

  • the present invention relates to modified growth surfaces used to grow biological
  • the present invention relates to diagnostic devices which employ
  • Such growth surfaces e.g., the commonly used dipslides or paddles.
  • Dipslides or paddles are microbial culture devices which contain agar medium. They are
  • liquid usually urine
  • slurries so that the agar medium comes in
  • microorganisms in the liquid exceeds approximately 100,000 - 1,000,000 colony forming
  • Diaslide device e.g., in Rosenberg
  • Another problem is the opposite problem, viz., that sometime the concentration of
  • microorganisms in the sampled liquid e.g., water, blood, urine, foodstuff
  • the sampled liquid e.g., water, blood, urine, foodstuff
  • the colonies growing on the surface are so few as to be barely or not at all discernible.
  • the present invention provides a dipslide that does not contain any additional or
  • invention comprises treating the growth surface so as to affect the liquid retention
  • the modification of the growth surface is achieved by coating the growth
  • cuts are provided in the growth surface so as to improve the liquid retention properties of
  • coating according to this preferred embodiment of the invention causes a greater part of the liquid coming into contact with the surface to run off it, thus leaving on the surface a
  • oils or a wax, or a mixture of both, other hydrophobic materials.
  • oils or a wax, or a mixture of both, other hydrophobic materials.
  • paraffin in a solvent selected from among acetone, ethyl acetate and pentane, the oily
  • the agar surface is generally diminished.
  • the slurry is preferably (but non limitatively) made by
  • liquid phases such as acetone or ethyl acetate
  • the coating material can be any suitable material which, upon drying, results in an
  • the coating material consists essentially of flour.
  • the coating material consists essentially of wax particles, e.g. carnauba wax, suspended in
  • the nutrient component e.g. in the agar
  • the surface does not have to be coated uniformly, and in fact, in
  • the growth surface is selectively
  • the coating of the surface is
  • a slurry containing a fine particulate can be applied to the agar surface in a
  • one portion of the agar can be treated (e.g., the bottom third), so that two very different
  • the agar can be dipped into the coating
  • the invention is also directed to growth surfaces modified by the method of the invention.
  • Such surfaces can be of different kinds, and the invention is in no way limited to
  • a fluid such as urine, or a
  • slurry such as homogenized foodstuff, is applied, forms a part of the present invention.
  • the invention further encompasses devices using the modified surface of the invention,
  • medium surfaces e.g., plating of vegetable, plant or animal cells.
  • Fig. 1 schematically illustrates results obtained on a prior art dipslide
  • Fig. 2 illustrates a dipslide partially coated at an angle with a coating that
  • Fig. 3 illustrates a dipslide coated with two different coatings
  • Fig. 4 illustrates a surface coated with a gradient of growth inhibiting coating.
  • silicone wax 9800 and silicone oil 350 both ex Goldschmidt,
  • Fig. 2 shows a slide which has been coated in its lower part (B), while upper part (A) has
  • This diagonal is useful particularly in samples containing a low concentration of

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Cette invention concerne un procédé de modification des propriétés de placage d'une surface de croissance, lequel procédé consiste à traiter ladite surface de croissance de manière à modifier ses propriétés de rétention de liquides et/ou les dimensions de sa surface.
PCT/IL1998/000213 1997-05-15 1998-05-07 Modification des proprietes de placage de surfaces de croissance WO1998051778A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU72310/98A AU7231098A (en) 1997-05-15 1998-05-07 Modification of the plating properties of growth surfaces

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL120833 1997-05-15
IL12083397A IL120833A0 (en) 1997-05-15 1997-05-15 Method for modifying the plating properties of a growth surface and growth surfaces modified thereby

Publications (1)

Publication Number Publication Date
WO1998051778A1 true WO1998051778A1 (fr) 1998-11-19

Family

ID=11070129

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL1998/000213 WO1998051778A1 (fr) 1997-05-15 1998-05-07 Modification des proprietes de placage de surfaces de croissance

Country Status (3)

Country Link
AU (1) AU7231098A (fr)
IL (1) IL120833A0 (fr)
WO (1) WO1998051778A1 (fr)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3875015A (en) * 1969-02-03 1975-04-01 Abbott Lab Applicator for uniformly applying a sample to bacterial growth media
US4801547A (en) * 1987-01-02 1989-01-31 Ramot University Authority For Applied Research And Industrial Development Ltd. Device for detecting presence of micro-organisms in a sample
EP0320348A1 (fr) * 1987-12-09 1989-06-14 In Vitro Scientific Products Inc. Bouteilles tournantes pour la culture de cellules tissulaires
DE4410718A1 (de) * 1994-03-28 1995-10-12 Bode Chemie Gmbh & Co Vorrichtung zur Keimzahlbestimmung
JPH0833475A (ja) * 1994-07-25 1996-02-06 Fuji Photo Film Co Ltd 付着性動物細胞の培養基体
US5538887A (en) * 1994-02-18 1996-07-23 Charlotte-Mecklenburg Hospital Authority Cell culture apparatus having smooth surface for cell growth thereon
GB2304732A (en) * 1995-08-31 1997-03-26 Ashby Scient Ltd Culture vessel comprising pitted growth substrate
WO1997012966A1 (fr) * 1995-10-06 1997-04-10 Corning Incorporated Films de silice colloidale pour la culture de cellules

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3875015A (en) * 1969-02-03 1975-04-01 Abbott Lab Applicator for uniformly applying a sample to bacterial growth media
US4801547A (en) * 1987-01-02 1989-01-31 Ramot University Authority For Applied Research And Industrial Development Ltd. Device for detecting presence of micro-organisms in a sample
EP0320348A1 (fr) * 1987-12-09 1989-06-14 In Vitro Scientific Products Inc. Bouteilles tournantes pour la culture de cellules tissulaires
US5538887A (en) * 1994-02-18 1996-07-23 Charlotte-Mecklenburg Hospital Authority Cell culture apparatus having smooth surface for cell growth thereon
DE4410718A1 (de) * 1994-03-28 1995-10-12 Bode Chemie Gmbh & Co Vorrichtung zur Keimzahlbestimmung
JPH0833475A (ja) * 1994-07-25 1996-02-06 Fuji Photo Film Co Ltd 付着性動物細胞の培養基体
GB2304732A (en) * 1995-08-31 1997-03-26 Ashby Scient Ltd Culture vessel comprising pitted growth substrate
WO1997012966A1 (fr) * 1995-10-06 1997-04-10 Corning Incorporated Films de silice colloidale pour la culture de cellules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Week 9615, Derwent World Patents Index; AN 96-145935, XP002078698, MATSUO SHIGERO ET AL.: "Culture medium for adhesive animal cells e.g. T-cells - comprises fixed bed of entangled fibres and culture bed containing neutral synthetic macromolecules e.g. ethylene-vinyl alcohol copolymer" *

Also Published As

Publication number Publication date
IL120833A0 (en) 1997-09-30
AU7231098A (en) 1998-12-08

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