WO1998050580A3 - Methode de determination du nombre de copies transgeniques dans les cellules modifiees par genie genetique - Google Patents

Methode de determination du nombre de copies transgeniques dans les cellules modifiees par genie genetique Download PDF

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Publication number
WO1998050580A3
WO1998050580A3 PCT/DE1998/001190 DE9801190W WO9850580A3 WO 1998050580 A3 WO1998050580 A3 WO 1998050580A3 DE 9801190 W DE9801190 W DE 9801190W WO 9850580 A3 WO9850580 A3 WO 9850580A3
Authority
WO
WIPO (PCT)
Prior art keywords
transmuted
copies
determining
genetically manipulated
manipulated cells
Prior art date
Application number
PCT/DE1998/001190
Other languages
German (de)
English (en)
Other versions
WO1998050580A2 (fr
Inventor
Christoph Fiehn
Nina Wettschureck
Alexandra Krauthoff
Original Assignee
Christoph Fiehn
Nina Wettschureck
Alexandra Krauthoff
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Christoph Fiehn, Nina Wettschureck, Alexandra Krauthoff filed Critical Christoph Fiehn
Priority to AU82086/98A priority Critical patent/AU8208698A/en
Publication of WO1998050580A2 publication Critical patent/WO1998050580A2/fr
Publication of WO1998050580A3 publication Critical patent/WO1998050580A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention porte sur une méthode de détermination du nombre de copies transgéniques dans des cellules modifiées par généie génétique. Elle porte également sur un procédé permettant de déterminer le nombre de cellules modifiées par génie génétique, à partir du nombre de copies transgéniques.
PCT/DE1998/001190 1997-05-02 1998-04-28 Methode de determination du nombre de copies transgeniques dans les cellules modifiees par genie genetique WO1998050580A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU82086/98A AU8208698A (en) 1997-05-02 1998-04-28 Method for determining the number of transmuted copies in genetically manipul ated cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19718705A DE19718705A1 (de) 1997-05-02 1997-05-02 Verfahren zur quantitativen Bestimmung der Anzahl von Transgenkopien in gentechnisch veränderten Zellen
DE19718705.6 1997-05-02

Publications (2)

Publication Number Publication Date
WO1998050580A2 WO1998050580A2 (fr) 1998-11-12
WO1998050580A3 true WO1998050580A3 (fr) 1999-02-04

Family

ID=7828519

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/001190 WO1998050580A2 (fr) 1997-05-02 1998-04-28 Methode de determination du nombre de copies transgeniques dans les cellules modifiees par genie genetique

Country Status (3)

Country Link
AU (1) AU8208698A (fr)
DE (1) DE19718705A1 (fr)
WO (1) WO1998050580A2 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1159452A1 (fr) * 1999-03-05 2001-12-05 Transgene S.A. FRAGMENT NUCLEOTIDIQUE, SONDE, AMORCE, REACTIF ET PROCEDE DE DETECTION D'UNE SEQUENCE NUCLEOTIDIQUE DERIVEE DE L'ORIGINE DE REPLICATION DE pBR322
HU0600159D0 (en) * 2006-02-23 2006-04-28 Mta Szegedi Biolog Koezpont Kvantitative pcr
DE102006021257A1 (de) * 2006-04-28 2007-11-08 Eberhard-Karls-Universität Tübingen Universitätsklinikum Nachweis von transgener DNA (t DNA)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023023A1 (fr) * 1993-04-06 1994-10-13 University Of Rochester Procede pour la mesure quantitative d'une expression de gene utilisant une transcription inverse et une amplification enzymatique du genome, multiplexe et competitive

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL 1983, BECK ET AL.: "TRANSPOSON Tn5 FRAGMENT ENCODING NEOMYCIN AND KANAMYCIN RESISTANCE AND A FRAGMENT OF THE READING FRAME OF A FURTHER PROTEIN", XP002080465 *
HO S N ET AL: "SITE-DIRECTED MUTAGENESIS BY OVERLAP EXTENSION USING THE POLYMERASE CHAIN REACTION", GENE, vol. 77, no. 1, 1 January 1989 (1989-01-01), pages 51 - 59, XP000272761 *
MORGAN R A ET AL: "HUMAN GENE THERAPY", ANNUAL REVIEW OF BIOCHEMISTRY, vol. 62, 1993, pages 191 - 217, XP002031972 *
REISCHL U ET AL: "QUANTITATIVE PCR A SURVEY OF THE PRESENT TECHNOLOGY", MOLECULAR BIOTECHNOLOGY, vol. 3, 1995, pages 55 - 71, XP000600241 *

Also Published As

Publication number Publication date
DE19718705A1 (de) 1998-11-05
AU8208698A (en) 1998-11-27
WO1998050580A2 (fr) 1998-11-12

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