WO1998042773A2 - Procede de greffage d'un sujet au moyen d'un film de greffage ameliore - Google Patents

Procede de greffage d'un sujet au moyen d'un film de greffage ameliore Download PDF

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Publication number
WO1998042773A2
WO1998042773A2 PCT/US1998/005248 US9805248W WO9842773A2 WO 1998042773 A2 WO1998042773 A2 WO 1998042773A2 US 9805248 W US9805248 W US 9805248W WO 9842773 A2 WO9842773 A2 WO 9842773A2
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WO
WIPO (PCT)
Prior art keywords
film
scion
graft union
rootstock
plant
Prior art date
Application number
PCT/US1998/005248
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English (en)
Other versions
WO1998042773A3 (fr
Inventor
Alexander P. Tereschenko
Jeffrey Duarte
Original Assignee
Duarte Nurseries, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/823,056 external-priority patent/US5832661A/en
Priority claimed from US08/823,057 external-priority patent/US5832662A/en
Application filed by Duarte Nurseries, Inc. filed Critical Duarte Nurseries, Inc.
Priority to AU64701/98A priority Critical patent/AU6470198A/en
Publication of WO1998042773A2 publication Critical patent/WO1998042773A2/fr
Publication of WO1998042773A3 publication Critical patent/WO1998042773A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
    • C08J5/18—Manufacture of films or sheets
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00—Vegetative propagation
    • A01G2/30—Grafting
    • A01G2/38—Holding; Ligating
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2323/00—Characterised by the use of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Derivatives of such polymers
    • C08J2323/02—Characterised by the use of homopolymers or copolymers of unsaturated aliphatic hydrocarbons having only one carbon-to-carbon double bond; Derivatives of such polymers not modified by chemical after treatment

Definitions

  • the present invention relates to a film for grafting a scion to a rootstock as well as methods and compositions in which the grafting film is used.
  • Grafting is a method of asexual plant reproduction involving the joining of a scion to a rootstock. Once grafted, water and nutrients are transported from the rootstock to the scion to support growth of the scion.
  • the complementary ends of the scion and rootstock are brought together to form a graft union.
  • Callous tissue forms at the graft union as part of the normal healing process of the plant and serves as a conduit for water and nutrients between the scion and rootstock.
  • the extent of the callous tissue formed at the graft union between the scion and rootstock, as well as the cell density of the callous tissue, is critical to the plant's ability to transmit water and nutrients from the rootstock to the scion and ultimately limits the size and rate at which the scion grows.
  • callous tissue formation is influenced by a variety of factors including temperature, light, pressure, moisture and chemical growth regulators.
  • a variety of different methods and tools have been developed for binding the cut ends of the scion and rootstock and for fostering callous tissue formation. These methods and tools generally involve a binding agent (e.g., staple, rubber or wax grafting bands) which brings the cut scion and rootstock ends into close contact. These methods and tools also generally include the use of a sealing agent (e.g., wax) which isolates the graft union from external elements and protects the graft union from dehydration, insects, and the environment.
  • a binding agent e.g., staple, rubber or wax grafting bands
  • a sealing agent e.g., wax
  • U.S. Patent Nos. 5,133,335 and 5,099,600 teach a method and apparatus in which a graft union is wrapped in budding strips and then covered with paraffin wax.
  • U.S. Patent No. 5,529,827 teaches the use of a self-adhesive film formed of a composite of paraffin wax, polyisobutylene and polyethylene. The self-adhesive film is covered by minute particles which are used to alter the self-adhesiveness of the film.
  • U.S. Patent No. 4,383,390 teaches a method for heating a graft union in order to foster callous tissue formation.
  • any grafting method it is important that the graft union formed between the scion and rootstock produce callous tissue which is capable of supporting strong and rapid scion growth. In this regard, it is important that callous tissue form throughout the graft union.
  • the grafting method should also be simple and inexpensive to practice.
  • the grafting method should enable a large number of grafts to be produced per unit time and is preferably suitable for automation. The method should be reliable and produce a high percentage of viable plants. A need currently exists for a grafting method and tool which provides the above-described advantages.
  • the present invention relates to a grafting film formed of a film of a polyalkylene polymer having a self-adhesive property and an elastic memory which causes the film when stretched around a scion and rootstock graft union to gradually contract around the graft union and apply an increasing amount of pressure circumferentially around the graft union.
  • the film also has oxygen and C0 2 permeability properties which cause a gas volume isolated by the film adjacent the graft union to have a higher C0 2 concentration and a lower oxygen concentration relative to the ambient environment around the plant.
  • the film preferably has at least 12% oxygen permeability and less than 3% C0 2 retention.
  • the film is preferably less than about 60 ⁇ m thick and more preferably less than about 50 ⁇ m thick.
  • the polyalkylene polymer film is formed of olefin subunits having between 1 and 6 carbons. Examples of these olefin subunits include, but are not limited to ethylene, propylene, isopropylene, butylene and isobutylene. In one embodiment, the polyalkylene polymer film is either polyethylene, polyisopropylene, polybutylene or polyisobutylene.
  • the present invention also relates to a method for forming a plant by grafting a scion to a rootstock. According to the method, an end of the scion and an end of the rootstock to be grafted are cut to form complementary ends. These ends are then joined together to form a graft union. A grafting film according to the present invention is then wrapped around the graft union.
  • the present invention also relates to a plant formed by grafting a scion to a rootstock in which the scion and rootstock are bound together using a grafting film of the present invention.
  • the present invention also relates to a method for growing root buds on a grafted plant.
  • a grafted plant including a rootstock and a scion grafted together is placed in a water bath for a first period of time such that the water covers the root buds to be formed on the rootstock.
  • the grafted plant is then removed from the water bath for a second period of time.
  • the steps of placing the rootstock in the water for a first period of time and removing the rootstock from the water for a second period of time is then repeated until root buds are formed on the plant.
  • the water used is at least partially replaced before each bathing of the rootstock in the water and is preferably completely replaced before each bathing of the rootstock in the water.
  • the grafted plant is preferably maintained in a vertical position during each bathing step and, more preferably, is maintained in a vertical position during the entire process.
  • the temperature of the water bath is preferably between 23°C and 32°C.
  • the ambient air temperature is preferably within about 5°C of the temperature of the water bath.
  • the air preferably has a relative humidity of at least 65%, more preferably between 65% and 95%.
  • the second period of time during which the rootstock is removed from the water bath is preferably twice as long as the period of time during which the rootstock is bathed in the water.
  • the rootstock is bathed in water for about 6 hours and removed from the water for another 18 hours during each cycle.
  • the water preferably contains IBA, preferably at a concentration of about 3%.
  • the graft union of the grafted plant is preferably wrapped in grafting film during root bud formation.
  • the grafting film is formed of a film of a polyalkylene polymer having a self-adhesive property and an elastic memory which causes the film when stretched around the scion and rootstock graft union to gradually contract around the graft union and apply an increasing amount of pressure circumferentially around the graft union.
  • the film also has oxygen and C0 2 permeability properties which cause a gas volume isolated by the film adjacent the graft union to have a higher C0 2 concentration and a lower oxygen concentration relative to the ambient environment around the plant.
  • the film preferably has at least 12% oxygen permeability and less than 3% C0 2 retention.
  • the film is preferably less than about 60 ⁇ m thick and more preferably less than about 50 ⁇ m thick.
  • the polyalkylene polymer film is formed of olefin subunits having between 1 and 6 carbons. Examples of these olefin subunits include, but are not limited to ethylene, propylene, isopropylene, butylene and isobutylene. In one embodiment, the polyalkylene polymer polymer film is either polyethylene, polyisopropylene, polybutylene or polyisobutylene.
  • Figure 1 A illustrates a scion and rootstock being cut such that the ends of the scion and rootstock are complementary.
  • Figure 1 B illustrates the scion and rootstock being brought into contact with each other such that the complementary ends of the scion and rootstock receive each other to form a graft union.
  • Figure 2 illustrates the scion and rootstock being wrapping by grafting film.
  • Figure 3 illustrates the grafting film being wrapped around the top of the scion.
  • Figure 4A illustrates a grafting union bound using a staple.
  • Figure 4B illustrates a graft union bound using grafting film of the present invention.
  • Figure 5A illustrate the amount of callous tissue formed by the graft illustrated in Figure 4A.
  • Figure 5B illustrate the amount of callous tissue formed by the graft illustrated in Figure 4B.
  • Figure 6A illustrates a cross-section of the graft union illustrated in Figure 4A which shows the amount of callous tissue formed by the graft.
  • Figure 6B illustrates a cross-section of the graft union illustrated in Figure 4B which shows the amount of callous tissue formed by the graft.
  • Figure 7A illustrates the cell density of callous tissue formed by the graft illustrated in Figure 4A.
  • Figure 7B illustrates the cell density of callous tissue formed by the graft illustrated in Figure 4B.
  • Figure 8 illustrates the functioning of the grafting film adjacent the graft union for controlling the transfer of water, C0 2 and oxygen.
  • Figures 9-11 illustrate the emergence of a bud formed by the graft through the grafting film.
  • Figure 9 illustrates a bud developing beneath the grafting film.
  • Figure 10 illustrates the tip of the bud piercing through the grafting film.
  • Figure 11 illustrates the growth of the bud through the grafting film.
  • Figures 12A and 12B illustrate a preferred method for growing root buds on grafted plants.
  • Figure 12A illustrates the step of placing grafted plants in water for a first period of time.
  • Figure 12B illustrates the step of removing the grafted plants from the water for a second period of time.
  • the present invention relates to a grafting film, a method for using the grafting film to rapidly and efficiently graft a scion to a rootstock, and the grafted plant that is produced using the grafting film and method.
  • the grafting film is used in the method of the present invention to form plants having enhanced callous tissue formation at the graft union.
  • the enhanced callous tissue formed using the grafting film and by the method supports excellent fluid and nutrient transport from the rootstock to the scion.
  • the callous tissue that is formed may be characterized by a higher cell density and a more highly ordered organization of cells which results in a more efficient physiological link between the scion and the rootstock.
  • plants grafted using the grafting film and according to the method exhibit more rapid and enhanced scion development.
  • the grafting film and method produce a higher percentage of viable plants as compared to previous methods.
  • the grafting film and method of the present invention may be used with all horticulture crops.
  • the grafting film and method are particularly well suited for grafts involving members of the citrus and prunus families of plants, and, in particular, grapevines.
  • the cambium layer in grapevines (as well as other plant varieties whose cambium layer is not perennial) is developed in a single year and does not grow after the first year.
  • the callous tissue that is developed between the scion and rootstock during grafting does not improve after the first year. It is therefore important to the viability and vigor of a grafted plant that the callous tissue formed during the grafting process form substantially circumferentially around the grafted plant at the graft union. It is also important that the callous tissue be formed of densely packed cells. This type of strong callous tissue growth is provided by the grafting film and method of the present invention.
  • the grafting film of the present invention has several physical properties which make the grafting film particularly well suited for inducing strong callous tissue growth. Callous tissue formation has been found to be enhanced by the presence of a slightly higher C0 2 concentration and slightly lower oxygen concentration than the ambient environment.
  • One property of the grafting film is its gas permeability which supports the formation of a gas layer under the grafting film adjacent the plant which has a slightly higher C0 2 concentration and slightly lower oxygen concentration than the ambient environment. More specifically, the grafting film is permeable to oxygen and C0 2 and preferably has an oxygen permeability of at least 12% and C0 2 retention of less than 3%.
  • the grafting film also assists the scion to retain water. As a result, the relative humidity of the gas layer under the film adjacent the plant is maintained at a level greater than 95%. The ability of the grafting film to retain water prevents the scion from dehydrating and further facilitates the growth of callous tissue.
  • the grafting film has a self-adhesive character which enables the film to be wrapped around the scion without unraveling.
  • This self- adhesive character is an important property since it is critical for the grafting film to remain wrapped around the graft union as the plant matures.
  • the grafting film is also elastic which enables the film to be stretched around a graft union.
  • the elastic nature of the film also enables the film to expand as the scion grows.
  • the grafting film also possesses elastic memory which causes the film to gradually contract around the graft union after being stretched. As the grafting film contracts, the film applies pressure circumferentially around the graft union. The pressure applied by the film is not immediate and gradually increases over the course of a few minutes. This gradual increase in pressure gives the scion and rootstock time to move relative to each other to a position where there any gap between the scion and rootstock is minimized.
  • rubber grafting strips apply an immediate pressure to the scion and rootstock which can prevent the scion and rootstock from settling into an optimal position relative to each other.
  • the elastic memory of the grafting film continues to apply pressure as the callous tissue grows. The cells in the callous tissue formed under this pressure are more densely packed and more highly organized. As a result, the callous tissue that is formed acts as a more efficient physiological conduit between the scion and rootstock as compared to less dense callous tissue.
  • the grafting film is preferably at least 50% light transmissive and is more preferably at least 95% light transmissive (transparent). Light has been found to be absorbed by chlorophyl in the callous which stimulates callous differentiation. The light transmissive nature of the grafting film allows light to reach the chlorophyl and be absorbed.
  • the film is colored (i.e., it reflects selected wavelengths of light). In a preferred embodiment, the film appears to have a light blue color.
  • the grafting film is also preferably thermally stable such that it does not decompose in direct sunlight.
  • the film is stable to a temperature of at least 300 °C. The thermal stability of the grafting film enables the film to be exposed to direct sunlight without decomposing or disassociating from the plant.
  • wax and paraffin based films have a low melting point which cause these films to decompose in direct sunlight. If the grafting film decomposes before the callous tissue is fully formed, the pressure being applied to the graft union is lost and less dense callous tissue is formed. In addition, cracks in the film due to film decomposition expose the graft union to the environment and allow water trapped in the scion to escape. Melted wax or paraffin can also seep into the graft union between the ends of the scion and rootstock and prevent the formation of callous tissue interconnecting the two.
  • the grafting film is preferably formed of a single polyalkylene polymer that is preferably formed of one or more polymers having olefin subunits between 2 and 6 carbons in length.
  • the polyalkylene polymer is preferably formed from polyethylene, polypropylene, polybutylene, polyisobutylene, or a mixture thereof.
  • the polyalkylene polymer preferably has a specific gravity between about 0.91 and 0.97.
  • the grafting film should be sufficiently thin that it does not impede scion growth.
  • the bud is covered by the grafting film which serves to protect the grafting film from infection by different microorganisms as well as to dehydration.
  • the grafting film should be sufficiently thin to allow the scion bud to expand beneath the film and ultimately pierce through the film.
  • the bud should be able to continue to grow unimpeded by the grafting film, stretching and pushing the grafting film aside as necessary. Meanwhile, a portion of the bud adjacent the scion should remain protected by the film.
  • the grafting film preferably has a thickness of less than about 60 ⁇ m, more preferably less than about 50 ⁇ m and is preferably between about 40-60 ⁇ m thick, more preferably between about 40-50 ⁇ m thick. At these thicknesses, the grafting film is sufficiently thin to allow a bud growing on the scion to pierce through the grafting film.
  • Figures 1-3 illustrate steps in a method for grafting a scion to a rootstock using the grafting film of the present invention.
  • a scion 12 and rootstock 14 are cut such that the ends 16, 18 of the scion 12 and rootstock 14 are complementary.
  • the scion 12 and rootstock 14 are brought into contact with each other such that the complementary ends 16, 18 receive each other to form a graft union 20.
  • FIG. 1A and 1B illustrates a cutting pattern for cutting complementary ends on the scion and rootstock. Rather, this invention relates to an improvement in the way in which any scion and rootstock are bound together using the grafting film of the present invention to induce enhanced callous tissue formation.
  • Figure 2 illustrates a scion 12 and rootstock 14 in the process of being wrapping by grafting film 22. As illustrated in Figure 2 by the arrows, the grafting film 22 applies pressure around the circumference of the graft union 22. This forces the ends 16, 18 of the scion 12 and rootstock 14 into contact with each other.
  • Figure 3 illustrates the grafting film 22 being wrapped around the top 24 of the scion 12. It is noted that the grafting film 22 is preferably wrapped over the entire scion in order to protect the scion 12 from infection, insects, changes in temperature and other factors which can harm the scion. By wrapping the entire scion 12, tender buds 26 that are later produced by the scion are also protected.
  • Figures 4A and 4B compare the graft union formed using a staple
  • a staple 30 is introduced into the body of the plant which forces one side 32 of the rootstock end 18 into contact with the scion end 16.
  • a gap 34 exists on the side 36 of the rootstock end 18 opposite the staple 30. This gap 34 exposes the graft union 20 to dehydration. Callous formation (not shown) adjacent the gap 34 is impeded by the existence of the gap 34, both due to dehydration and the lack of contact between the scion and rootstock.
  • the use of a staple is also disadvantageous because it involves the introduction of a foreign object into the body of the plant.
  • the graft union 20 formed through the use of grafting film 22 in Figure 4B does not include the gap 34 between the scion end 16 and rootstock end 18 illustrated in Figure 4A.
  • denser callous tissue is formed using the grafting film than a staple.
  • callous tissue is formed over a greater percentage of the area between the scion and rootstock ends when the grafting film of the present invention is used.
  • Figures 5A and 5B illustrate the different amounts of callous tissue 38 formed by the grafts illustrated in Figures 4A and 4B. As illustrated in Figure 5A, callous tissue 38 is not formed throughout the graft union 20 between the scion 12 and rootstock 14.
  • callous tissue 38 is formed over more of the area between the scion 12 and rootstock 14 when the grafting film 22 is used.
  • callous tissue is formed around the circumference of the rootstock when grafting film is used.
  • Figures 6A and 6B illustrate a cross-section of the graft union 20 which shows the different amount of callous tissue 38 formed by the grafts illustrated in Figures 4A and 4B. As illustrated in Figures 6A and 6B
  • the concentration of callous tissue 38 formed is greater when grafting film is used to bind the scion and rootstock.
  • Figures 7A and 7B illustrate the density of the cells 40 of callous tissue 38 formed by the grafts illustrated in Figures 4A and 4B. As illustrated in Figures 7A and 7B, the density of cells in the callous tissue
  • Figure 8 illustrates the functioning of the grafting film 22 adjacent the graft union 20 as a water, C0 2 and oxygen transfer medium.
  • the grafting film preferably has low level C0 2 retention, preferably less than about 3% C0 2 retention.
  • the grafting film also preferably has a high degree of oxygen permeability, preferably at least 12%.
  • the local environment adjacent the graft union has a slightly higher concentration of C0 2 and a slightly lower concentration of oxygen than the surrounding atmosphere, preferably 16% of 0 2 and 2% of C0 2 .
  • This slightly higher C0 2 concentration and lower oxygen concentration is believed to further facilitate the growth of callous tissue.
  • the grafting film assists the graft to retain water, thereby reducing the risk of dehydration at the graft union which can also impede the growth of callous tissue.
  • Figures 9-11 illustrate the emergence of a bud 26 formed by the graft through the grafting film 22.
  • Figure 9 illustrates a bud 26 developing beneath the grafting film 22.
  • Figure 10 illustrates the tip of the bud 26 piercing through the grafting film 26.
  • Figure 11 illustrates the growth of the bud 26 through the grafting film.
  • the grafting film 20 should be sufficiently thin and elastic to allow a bud 26 to develop under the grafting film 22 and ultimately break through the grafting film 22.
  • the grafting film should also be sufficiently thin and elastic to allow the bud and scion to grow without being restricted by the grafting film, thereby avoiding the need to remove the grafting tape after callous formation.
  • the grafting film preferably has a thickness of less than about
  • FIGS 12A and 12B illustrate a preferred method for growing root buds on grafted plants. As illustrated in Figure 12A, the grafted plants 44 are placed in water for a first period of time. The water should be sufficiently deep to cover the root buds to be formed. During this time, the plants are preferably held in a vertical position. As illustrated in FIG. 12A, the grafted plants 44 are placed in water for a first period of time. The water should be sufficiently deep to cover the root buds to be formed. During this time, the plants are preferably held in a vertical position. As illustrated in Figure 12A, the grafted plants 44 are placed in water for a first period of time. The water should be sufficiently deep to cover the root buds to be formed. During this time, the plants are preferably held in a vertical position. As illustrated in FIG. 12A, the grafted plants 44 are placed in water for a first period of time. The water should be sufficiently deep to cover the root buds to be formed. During this time, the plants are preferably held in a vertical position. As illustrated
  • bathing the plants in water may be accomplished by placing the plants in a water bath.
  • the water level in the bath is preferably about 2 inches deep.
  • the air temperature during this first period of time when the plants are being bathed is preferably between about 23°C and 32°C with a relative humidity of between about
  • the water temperature is preferably within about 5°C of the air temperature.
  • the grafted plants 44 are removed from the water after the first period of time for a second period of time. This may be achieved by removing the plants from the water bath or, more preferably, by removing the water from the water bath 46.
  • the timing for placing the plants into and out of the water varies depending on the particular plant variety. For grapevines, plants are commonly placed in water for about 6 hours and removed from the water for about 18 hours. During the time that the plants are not in water, the plants are preferably held in a vertical position.
  • the air temperature during this second period of time is preferably between about 23°C and 32°C and has a relative humidity of 65% to 95%.

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Manufacturing & Machinery (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Materials Engineering (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Laminated Bodies (AREA)
  • Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)

Abstract

L'invention concerne un film de greffage (22), constitué d'un film (22) d'un polymère polyalkylène présentant un pouvoir auto-adhésif et une mémoire élastique permettant audit film (22), lorsque celui-ci est enroulé autour d'un greffon (12) et du point de greffe (20) d'un sujet, de se contracter progressivement autour dudit point de greffe (20), afin appliquant ainsi une pression croissante sur la circonférence de ce point de greffe (20). Ce film (22) présente également un pouvoir de perméabilité à l'oxygène et au CO2, un volume de gaz isolé par le film (22) adjacent au point de greffe (20) présentant ainsi une concentration en CO2 supérieure et une concentration en oxygène inférieure à celles de l'environnement dans lequel se trouve la plante. Le film (22) présente de préférence une perméabilité à l'oxygène au moins égale à 12 %, et une rétention de CO2 inférieure à 3 %. L'épaisseur de ce film (22) est de préférence inférieure à 60 νm, et idéalement inférieure à 50 νm environ.
PCT/US1998/005248 1997-03-21 1998-03-17 Procede de greffage d'un sujet au moyen d'un film de greffage ameliore WO1998042773A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU64701/98A AU6470198A (en) 1997-03-21 1998-03-17 Method for grafting rootstock using improved grafting film

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/823,056 US5832661A (en) 1997-03-21 1997-03-21 Method for growing root buds
US08/823,057 US5832662A (en) 1997-03-21 1997-03-21 Method for grafting rootstock using improved grafting film
US08/823,056 1997-03-21
US08/823,057 1997-03-21

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WO1998042773A2 true WO1998042773A2 (fr) 1998-10-01
WO1998042773A3 WO1998042773A3 (fr) 1999-05-20

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4098020A (en) 1976-01-29 1978-07-04 Cook Edward J Method and apparatus for horticultural grafting
US4392304A (en) 1980-05-20 1983-07-12 Aranykalasz Mgtsz Universal manual grafting device
US4467559A (en) 1981-10-08 1984-08-28 Techsearch, Inc. Chip budding machine
US4654999A (en) 1981-12-10 1987-04-07 Raggett William C P Cutting device
US4769944A (en) 1986-01-27 1988-09-13 Groupement Champenois D'exploitation Grafting machine
US5209011A (en) 1989-01-19 1993-05-11 Kabushiki Kaisha Komatsu Seisakusho Apparatus for grafting immature plants
US5414958A (en) 1992-09-24 1995-05-16 Iwatani Sangyo Kabushiki Kaisha Method and apparatus for uniting a scion to a stock

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Publication number Priority date Publication date Assignee Title
JPS53127125A (en) * 1977-04-11 1978-11-07 Moon Star Chemical Corp Grafting method and composition for grafting
JPS5911120A (ja) * 1982-07-12 1984-01-20 三井東圧化学株式会社 接木方法
FR2613581B1 (fr) * 1987-04-08 1990-12-14 Grp Champenois Expl Vitic Procede de production de plants greffes de vegetaux ligneux ou semi-ligneux pour l'obtention de plants, notamment de vigne, et plants obtenus
IT1211705B (it) * 1987-08-10 1989-11-03 Patrucco Sergio Procedimento di moltiplicazione di varieta' vegetali, e prodotti cosi'ottenuti
US5099600A (en) * 1989-01-17 1992-03-31 Champion International Corporation Paraffin wax grafting technique and apparatus
JPH0523050A (ja) * 1991-07-16 1993-02-02 Daiseru Kanzaki Kako Kk 接ぎ木用テープ
JP2855595B2 (ja) * 1993-08-26 1999-02-10 株式会社アグリス 自己粘着性フィルム

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4098020A (en) 1976-01-29 1978-07-04 Cook Edward J Method and apparatus for horticultural grafting
US4392304A (en) 1980-05-20 1983-07-12 Aranykalasz Mgtsz Universal manual grafting device
US4467559A (en) 1981-10-08 1984-08-28 Techsearch, Inc. Chip budding machine
US4654999A (en) 1981-12-10 1987-04-07 Raggett William C P Cutting device
US4769944A (en) 1986-01-27 1988-09-13 Groupement Champenois D'exploitation Grafting machine
US5209011A (en) 1989-01-19 1993-05-11 Kabushiki Kaisha Komatsu Seisakusho Apparatus for grafting immature plants
US5414958A (en) 1992-09-24 1995-05-16 Iwatani Sangyo Kabushiki Kaisha Method and apparatus for uniting a scion to a stock
US5524386A (en) 1992-09-24 1996-06-11 Iwatani Sangyo Kabushiki Kaisha Method and apparatus for uniting a scion to a stock

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AR011200A1 (es) 2000-08-02
WO1998042773A3 (fr) 1999-05-20
AU6470198A (en) 1998-10-20

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