WO1998037175A1 - Method of constructing vectors for homologous recombination directed mutagenesis - Google Patents

Method of constructing vectors for homologous recombination directed mutagenesis Download PDF

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Publication number
WO1998037175A1
WO1998037175A1 PCT/US1998/003243 US9803243W WO9837175A1 WO 1998037175 A1 WO1998037175 A1 WO 1998037175A1 US 9803243 W US9803243 W US 9803243W WO 9837175 A1 WO9837175 A1 WO 9837175A1
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WO
WIPO (PCT)
Prior art keywords
vector
sequences
genomic dna
selection cassette
positive selection
Prior art date
Application number
PCT/US1998/003243
Other languages
English (en)
French (fr)
Inventor
Michael Nehls
Sigrid Wattler
Original Assignee
Michael Nehls
Sigrid Wattler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Michael Nehls, Sigrid Wattler filed Critical Michael Nehls
Priority to JP53687598A priority Critical patent/JP2002514072A/ja
Priority to US09/171,642 priority patent/US6924146B1/en
Priority to EP98908603A priority patent/EP0912722A4/de
Publication of WO1998037175A1 publication Critical patent/WO1998037175A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PCT/US1998/003243 1997-02-21 1998-02-20 Method of constructing vectors for homologous recombination directed mutagenesis WO1998037175A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP53687598A JP2002514072A (ja) 1997-02-21 1998-02-20 突然変異誘発を指示する相同組み換えのためのベクターの構築の方法
US09/171,642 US6924146B1 (en) 1997-02-21 1998-02-20 Method of constructing vectors for homologous recombination directed mutagenesis
EP98908603A EP0912722A4 (de) 1997-02-21 1998-02-20 Verfahren zur konstruktion von vektoren zur homologen rekombination durch gerichtete mutagenese

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19707012.4 1997-02-21
DE19707012 1997-02-21

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/171,642 A-371-Of-International US6924146B1 (en) 1997-02-21 1998-02-20 Method of constructing vectors for homologous recombination directed mutagenesis
US10/636,822 Continuation US20040043487A1 (en) 1997-02-21 2003-08-06 Method of constructing vectors for homologous recombination directed mutagenesis

Publications (1)

Publication Number Publication Date
WO1998037175A1 true WO1998037175A1 (en) 1998-08-27

Family

ID=7821111

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/003243 WO1998037175A1 (en) 1997-02-21 1998-02-20 Method of constructing vectors for homologous recombination directed mutagenesis

Country Status (3)

Country Link
EP (1) EP0912722A4 (de)
JP (1) JP2002514072A (de)
WO (1) WO1998037175A1 (de)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000055311A2 (en) * 1999-03-17 2000-09-21 E.I. Du Pont De Nemours And Company Gene modification by homologous recombination
WO2001075127A2 (de) * 2000-04-03 2001-10-11 Ingenium Pharmaceuticals Ag Klonierungssystem zur konstruktion von homologen rekombinationsvektoren
WO2002014508A2 (en) * 2000-08-15 2002-02-21 Lexicon Genetics Incorporated Arrayed collection of genomic clones
EP1184461A2 (de) * 2000-08-25 2002-03-06 Pfizer Products Inc. Konstruktion von Vektoren zur zielgerichteten Änderung von Genen durch homologe Rekombination in Hefe
US6376192B1 (en) 2000-11-28 2002-04-23 Baylor College Of Medicine Method for screening of DNA libraries and generation of recombinant DNA constructs
WO2003006663A2 (de) * 2001-07-12 2003-01-23 Bayer Cropscience Ag Verfahren zur herstellung von deletionsmutanten
US6548271B1 (en) 1999-04-22 2003-04-15 Lexicon Genetics Incorporated Nucleic acids encoding human transporter proteins
US6579715B1 (en) 1999-01-19 2003-06-17 Lexicon Genetics Incorporated Mammalian cortexin-like proteins and polynucleotides encoding the same
US6806083B2 (en) 1999-10-12 2004-10-19 Lexicon Genetics Incorporated Human LDL receptor encoding polynucleotides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014769A1 (en) * 1993-11-29 1995-06-01 University Of South Florida TRANSGENIC MOUSE PREPARED USING YEAST ARTIFICIAL CHROMOSOMES (YACs) AND HOMOLOGOUS RECOMBINATION

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
COURTEMANCHE C., ET AL.: "SHUTTLE-VECTOR MUTAGENESIS BY AFLATOXIN B1 IN HUMAN CELLS: EFFECTS OF SEQUENCE CONTEXT ON THE SUPF MUTATIONAL SPECTRUM.", MUTATION RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 306., 1 January 1994 (1994-01-01), AMSTERDAM, NL, pages 143 - 151., XP002910505, ISSN: 0027-5107, DOI: 10.1016/0027-5107(94)90025-6 *
DOLLE M. E. T., ET AL.: "EVALUATION OF A PLASMID-BASED TRANSGENIC MOUSE MODEL FOR DETECTING IN VIVO MUTATIONS.", MUTAGENESIS., IRL PRESS, OXFORD., GB, vol. 11., no. 01., 1 January 1996 (1996-01-01), GB, pages 111 - 118., XP002910503, ISSN: 0267-8357 *
LEACH E. G., ET AL.: "TISSUE SPECIFICATION OF SPONTANEOUS POINT MUTATIONS IN LAMBDASUPF TRANSGENIC MICE.", ENVIRONMENTAL AND MOLECULAR MUTAGENESIS., WILEY-LISS, INC. CHICHESTER., GB, vol. 28., 1 January 1996 (1996-01-01), GB, pages 459 - 464., XP002910506, ISSN: 0893-6692, DOI: 10.1002/(SICI)1098-2280(1996)28:4<459::AID-EM23>3.0.CO;2-D *
LUNDBERG K. S., ET AL.: "THE USE OF SELECTION IN RECOVERY OF TRANSGENIC TARGETS FOR MUTATION ANALYSIS.", MUTATION RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 301., 1 January 1993 (1993-01-01), AMSTERDAM, NL, pages 99 - 105., XP002910508, ISSN: 0027-5107, DOI: 10.1016/0165-7992(93)90031-P *
MARTUS H.-J., ET AL.: "USE OF TRANSGENIC MOUSE MODELS FOR STUDYING SOMATIC MUTATIONS IN AGING.", MUTATION RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 338., 1 January 1995 (1995-01-01), AMSTERDAM, NL, pages 203 - 213., XP002910504, ISSN: 0027-5107, DOI: 10.1016/0921-8734(95)00025-2 *
See also references of EP0912722A4 *
STUART G. R., ET AL.: "THE GENETIC ANALYSIS OF LACL MUTATIONS IN SECTORED PLAQUES FROM BIG BLUE TRANSGENIC MICE.", ENVIRONMENTAL AND MOLECULAR MUTAGENESIS., WILEY-LISS, INC. CHICHESTER., GB, vol. 28., 1 January 1996 (1996-01-01), GB, pages 385 - 392., XP002910507, ISSN: 0893-6692, DOI: 10.1002/(SICI)1098-2280(1996)28:4<385::AID-EM12>3.0.CO;2-B *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6579715B1 (en) 1999-01-19 2003-06-17 Lexicon Genetics Incorporated Mammalian cortexin-like proteins and polynucleotides encoding the same
WO2000055311A3 (en) * 1999-03-17 2001-02-08 Du Pont Gene modification by homologous recombination
WO2000055311A2 (en) * 1999-03-17 2000-09-21 E.I. Du Pont De Nemours And Company Gene modification by homologous recombination
US6548271B1 (en) 1999-04-22 2003-04-15 Lexicon Genetics Incorporated Nucleic acids encoding human transporter proteins
US6806083B2 (en) 1999-10-12 2004-10-19 Lexicon Genetics Incorporated Human LDL receptor encoding polynucleotides
WO2001075127A2 (de) * 2000-04-03 2001-10-11 Ingenium Pharmaceuticals Ag Klonierungssystem zur konstruktion von homologen rekombinationsvektoren
WO2001075127A3 (de) * 2000-04-03 2002-02-07 Ingenium Pharmaceuticals Ag Klonierungssystem zur konstruktion von homologen rekombinationsvektoren
WO2002014508A2 (en) * 2000-08-15 2002-02-21 Lexicon Genetics Incorporated Arrayed collection of genomic clones
WO2002014508A3 (en) * 2000-08-15 2002-08-22 Lexicon Genetics Inc Arrayed collection of genomic clones
JP2004512829A (ja) * 2000-08-15 2004-04-30 レキシコン・ジェネティクス・インコーポレーテッド ゲノムクローンのアレイコレクション
EP1184461A3 (de) * 2000-08-25 2002-05-22 Pfizer Products Inc. Konstruktion von Vektoren zur zielgerichteten Änderung von Genen durch homologe Rekombination in Hefe
EP1184461A2 (de) * 2000-08-25 2002-03-06 Pfizer Products Inc. Konstruktion von Vektoren zur zielgerichteten Änderung von Genen durch homologe Rekombination in Hefe
SG164274A1 (en) * 2000-08-25 2010-09-29 Pfizer Prod Inc Rapid creation of gene targeting vectors using homologous recombination in yeast
US6376192B1 (en) 2000-11-28 2002-04-23 Baylor College Of Medicine Method for screening of DNA libraries and generation of recombinant DNA constructs
WO2003006663A2 (de) * 2001-07-12 2003-01-23 Bayer Cropscience Ag Verfahren zur herstellung von deletionsmutanten
EP1279741A1 (de) * 2001-07-12 2003-01-29 Bayer CropScience AG Verfahren zum Herstellen von Deletionsmutanten
WO2003006663A3 (de) * 2001-07-12 2003-09-12 Bayer Cropscience Ag Verfahren zur herstellung von deletionsmutanten

Also Published As

Publication number Publication date
EP0912722A4 (de) 2001-12-05
JP2002514072A (ja) 2002-05-14
EP0912722A1 (de) 1999-05-06

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