WO2000055311A3 - Gene modification by homologous recombination - Google Patents

Gene modification by homologous recombination Download PDF

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Publication number
WO2000055311A3
WO2000055311A3 PCT/US2000/006959 US0006959W WO0055311A3 WO 2000055311 A3 WO2000055311 A3 WO 2000055311A3 US 0006959 W US0006959 W US 0006959W WO 0055311 A3 WO0055311 A3 WO 0055311A3
Authority
WO
WIPO (PCT)
Prior art keywords
modification
plasmid
cassette
target gene
homologous recombination
Prior art date
Application number
PCT/US2000/006959
Other languages
French (fr)
Other versions
WO2000055311A2 (en
Inventor
Todd Michael Dezwaan
James Alan Sweigard
Original Assignee
Du Pont
Todd Michael Dezwaan
James Alan Sweigard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Todd Michael Dezwaan, James Alan Sweigard filed Critical Du Pont
Publication of WO2000055311A2 publication Critical patent/WO2000055311A2/en
Publication of WO2000055311A3 publication Critical patent/WO2000055311A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast

Abstract

A method for the modification of a specific gene of interest is described. The method employs two genetic constructs. One construct is a modification plasmid comprising the target gene or nucleic acid fragment of interest. The second construct is a modification cassette comprising yeast selectable markers, and optionally other DNA, bounded by flanking DNA which is complementary to portions of the target gene residing on the modification plasmid. Both constructs are transformed into budding yeast where homologous recombination occurs between the cassette and the plasmid. The resulting altered modification plasmid contains an insertion in the target gene in the regions governed by the sequence of the cassette flanking DNA.
PCT/US2000/006959 1999-03-17 2000-03-16 Gene modification by homologous recombination WO2000055311A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12487699P 1999-03-17 1999-03-17
US60/124,876 1999-03-17

Publications (2)

Publication Number Publication Date
WO2000055311A2 WO2000055311A2 (en) 2000-09-21
WO2000055311A3 true WO2000055311A3 (en) 2001-02-08

Family

ID=22417232

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/006959 WO2000055311A2 (en) 1999-03-17 2000-03-16 Gene modification by homologous recombination

Country Status (1)

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WO (1) WO2000055311A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107794257B (en) * 2016-08-31 2022-05-17 浙江安诺优达生物科技有限公司 Construction method and application of DNA large fragment library
CN107988218B (en) * 2016-10-25 2020-06-23 中国种子集团有限公司 Rice genome recombinant nucleic acid fragment RecCR012069 and detection method thereof
CN110305886A (en) * 2019-06-18 2019-10-08 南京师范大学 A kind of method of rapid build filamentous fungi expression vector

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998028416A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S In vivo recombination
WO1998037175A1 (en) * 1997-02-21 1998-08-27 Michael Nehls Method of constructing vectors for homologous recombination directed mutagenesis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998028416A1 (en) * 1996-12-20 1998-07-02 Novo Nordisk A/S In vivo recombination
WO1998037175A1 (en) * 1997-02-21 1998-08-27 Michael Nehls Method of constructing vectors for homologous recombination directed mutagenesis

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
CHEREPANOV P P ET AL: "Gene disruption in Escherichia coli: Tc and Km cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant", GENE,NL,ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, vol. 158, no. 1, 1995, pages 9 - 14, XP004206666, ISSN: 0378-1119 *
HOHN T M ET AL: "ISOLATION AND GENE DISRUPTION OF THE TOX5 GENE ENCODING TRICHODIENE SYNTHASE IN GIBBERELLA PULICARIS", MOLECULAR PLANT-MICROBE INTERACTIONS,US,APS PRESS, ST. PAUL, MN, vol. 5, no. 3, 1992, pages 249 - 256, XP000862963, ISSN: 0894-0282 *
LIU SHAOHUA ET AL: "G Protein alpha subunit genes control growth, development, and pathogenicity of Magnaporthe grisea.", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 10, no. 9, December 1997 (1997-12-01), pages 1075 - 1086, XP000949327, ISSN: 0894-0282 *
ROSS-MACDONALD PETRA ET AL: "A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 94, no. 1, 1997, 1997, pages 190 - 195, XP002148084, ISSN: 0027-8424 *
SAUER B: "FUNCTIONAL EXPRESSION OF THE CRE-LOX SITE-SPECIFIC RECOMBINATION SYSTEM IN THE YEAST SACCHAROMYCES CEREVISIAE", MOLECULAR AND CELLULAR BIOLOGY,US,WASHINGTON, DC, vol. 7, no. 6, June 1987 (1987-06-01), pages 2087 - 2096, XP000923364, ISSN: 0270-7306 *
SAUER B: "MULTIPLEX CRE/IOX RECOMBINATION PERMITS SELECTIVE SITE-SPECIFIC DNATARGETING TO BOTH A NATURAL AND AN ENGINEERED SITE IN THE YEAST GENOME", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 24, no. 23, 1996, pages 4608 - 4613, XP002913183, ISSN: 0305-1048 *
SWEIGARD J A ET AL: "Magnaporthe grisea pathogenicity genes obtained through insertional mutagenesis", MOLECULAR PLANT-MICROBE INTERACTIONS,US,APS PRESS, ST. PAUL, MN, vol. 11, no. 5, 1 May 1998 (1998-05-01), pages 404 - 412, XP002091580, ISSN: 0894-0282 *
WESTPHAL CHRISTOPH HEINER ET AL: "Transposon-generated 'knock-out' and 'knock-in' gene-targeting constructs for use in mice", CURRENT BIOLOGY,CURRENT SCIENCE,,GB, vol. 7, no. 7, 1997, pages 530 - 533, XP000938425, ISSN: 0960-9822 *

Also Published As

Publication number Publication date
WO2000055311A2 (en) 2000-09-21

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