WO2002083889A3 - Methods for the production of minicircles - Google Patents

Methods for the production of minicircles Download PDF

Info

Publication number
WO2002083889A3
WO2002083889A3 PCT/GB2002/001668 GB0201668W WO02083889A3 WO 2002083889 A3 WO2002083889 A3 WO 2002083889A3 GB 0201668 W GB0201668 W GB 0201668W WO 02083889 A3 WO02083889 A3 WO 02083889A3
Authority
WO
WIPO (PCT)
Prior art keywords
recombination
site
minicircle
enzyme
modified
Prior art date
Application number
PCT/GB2002/001668
Other languages
French (fr)
Other versions
WO2002083889A2 (en
Inventor
Brian W Bigger
Oleg Tolmachov
Charles Coutelle
Original Assignee
Imp College Innovations Ltd
Brian W Bigger
Oleg Tolmachov
Charles Coutelle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0108968.9A external-priority patent/GB0108968D0/en
Application filed by Imp College Innovations Ltd, Brian W Bigger, Oleg Tolmachov, Charles Coutelle filed Critical Imp College Innovations Ltd
Priority to AU2002247848A priority Critical patent/AU2002247848A1/en
Publication of WO2002083889A2 publication Critical patent/WO2002083889A2/en
Publication of WO2002083889A3 publication Critical patent/WO2002083889A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts

Abstract

The present invention relates to a method for the production of a minicircle. In the method of the present invention, a parent plasmid is provided which has a nucleic sequence flanked by recombination sites. This parent plasmid is exposed to an enzyme which causes recombination at the recombination sites, thereby to form a (I) minicircle comprising the nucleic acid sequence and (ii) a miniplasmid comprising the remainder of the parent plasmid. One recombination site is modified at the 5' end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3' end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3' end such that its reaction with the enzyme is less efficient than the wild type site, both modified sites being located in the minicircle after recombination. This favours the formation of minicircle.
PCT/GB2002/001668 2001-04-10 2002-04-10 Methods for the production of minicircles WO2002083889A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002247848A AU2002247848A1 (en) 2001-04-10 2002-04-10 Methods for the production of minicircles

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0108968.9A GB0108968D0 (en) 2001-04-10 2001-04-10 Methods
GB0108968.9 2001-04-10
US32702901P 2001-10-05 2001-10-05
US60/327,029 2001-10-05

Publications (2)

Publication Number Publication Date
WO2002083889A2 WO2002083889A2 (en) 2002-10-24
WO2002083889A3 true WO2002083889A3 (en) 2003-11-13

Family

ID=26245953

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/001668 WO2002083889A2 (en) 2001-04-10 2002-04-10 Methods for the production of minicircles

Country Status (2)

Country Link
AU (1) AU2002247848A1 (en)
WO (1) WO2002083889A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7517688B2 (en) 2000-02-25 2009-04-14 Stratagene California Method for ligating nucleic acids and molecular cloning
IL166612A0 (en) 2002-08-29 2006-01-15 Univ Leland Stanford Junior Circular nucleic acid vectors and methods for making and using the same
AT412400B (en) 2003-05-08 2005-02-25 Mayrhofer Peter Mag Dr MINI CIRCLE MANUFACTURING
US7622252B2 (en) 2005-06-10 2009-11-24 Baylor College Of Medicine Generation of minicircle DNA with physiological supercoiling
US8921072B2 (en) * 2008-09-02 2014-12-30 General Electric Compnay Methods to generate DNA mini-circles
CA2777835A1 (en) 2009-10-16 2011-04-21 Baylor College Of Medicine Supercoiled minicircle dna for gene therapy applications
GB201307075D0 (en) 2013-04-19 2013-05-29 Mayrhofer Peter Plasmid for minicircle production
PT107663B (en) * 2014-05-28 2019-02-26 Inst Superior Tecnico MINI-CIRCLE PURIFICATION PROCESS
US20180305701A1 (en) 2017-03-14 2018-10-25 Baylor College Of Medicine Targeting minivectors to specific tissue using shape
US20200048716A1 (en) 2017-11-03 2020-02-13 Twister Biotech, Inc Using minivectors to treat ovarian cancer
US20240093226A1 (en) 2021-09-10 2024-03-21 Baylor College Of Medicine Ultrapure minivectors for gene therapy
US20240100189A1 (en) 2021-09-10 2024-03-28 Twister Biotech, Inc Using minivectors to treat idiopathic pulmonary fibrosis
US20230190955A1 (en) 2021-10-21 2023-06-22 Baylor College Of Medicine Treatment of liver cancer or liver fibrosis

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009127A2 (en) * 1992-10-16 1994-04-28 The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services Supercoiled minicircle dna as as a unitary promoter vector
EP1035208A1 (en) * 1997-11-13 2000-09-13 Sumitomo Pharmaceuticals Company, Limited VARIANT loxP SEQUENCES AND APPLICATION OF THE SAME
US6143530A (en) * 1995-02-23 2000-11-07 Rhone-Poulenc Rorer S.A. Circular DNA expression cassettes for in vivo gene transfer

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994009127A2 (en) * 1992-10-16 1994-04-28 The Government Of The United States Of America As Represented By The Secretary, Department Of Healthand Human Services Supercoiled minicircle dna as as a unitary promoter vector
US6143530A (en) * 1995-02-23 2000-11-07 Rhone-Poulenc Rorer S.A. Circular DNA expression cassettes for in vivo gene transfer
EP1035208A1 (en) * 1997-11-13 2000-09-13 Sumitomo Pharmaceuticals Company, Limited VARIANT loxP SEQUENCES AND APPLICATION OF THE SAME

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BIGGER B ET AL: "Efficient gene delivery minicircles, produced by the excision of bacterial sequences from plasmid vectors using Cre/loxP mediated recombination in vivo", THE JOURNAL OF GENE MEDECINE, vol. 2, no. 5 suppl, 2000, pages 160, XP002235520 *
DARQUET A-M ET AL: "A NEW DNA VEHICLE FOR NONVIRAL GENE DELIVERY: SUPERCOILED MINICIRCLE", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, VOL. 4, NR. 12, PAGE(S) 1341-1349, ISSN: 0969-7128, XP001000963 *
DARQUET A-M ET AL: "MINICIRCLE: AN IMPROVED DNA MOLECULE FOR IN VITRO AND IN VIVO GENE TRANSFER", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, VOL. 6, NR. 2, PAGE(S) 209-218, ISSN: 0969-7128, XP001000924 *

Also Published As

Publication number Publication date
AU2002247848A1 (en) 2002-10-28
WO2002083889A2 (en) 2002-10-24

Similar Documents

Publication Publication Date Title
WO2002083889A3 (en) Methods for the production of minicircles
WO2004015107A3 (en) Further novel forms of interfering rna molecules
WO2005061720A3 (en) High lysine maize compositions and methods for detection thereof
WO2001068836A3 (en) Methods and compositions for rna interference
WO2003062394A3 (en) Methods and compositions for rna interference
MXPA01011871A (en) Improved method for agrobacterium.
CA2263716A1 (en) Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase
WO2004031376A3 (en) Sesquiterpene synthases and methods of use
WO2004048255A3 (en) Dispersion of carbon nanotubes by nucleic acids
WO2001004314A3 (en) Nucleic acid molecules and proteins associated with sterol synthesis and metabolism
WO2000008169A8 (en) Dna sequence coding for a 1-deoxy-d-xylulose-5-phosphate synthase and the overproduction thereof in plants
WO2005110067A3 (en) Method and compositions for rna interference
WO2005005643A3 (en) Chloroplast transformation of duckweed
WO2004065600A3 (en) Rna interference by palindromic or modified rna molecules
WO2009104094A3 (en) Method of nucleic acid recombination
CN111321143A (en) Method for preparing circular RNA
PL1608752T3 (en) Method for isolating nucleic acids
WO2001081600A3 (en) Transgenic plants
WO2006012985A3 (en) Method for production of transgenic plants with increased pathogenic resistance by altering the content and/or activity of actin-depolymerising factors
WO2007144508A3 (en) Chimeric transfer rna and use thereof for the production of rna by a cell
WO2003027261A3 (en) Methods and compositions for gene targeting by homologous recombination
WO2001085909A3 (en) Development of plant based biological sensors and novel disease resistance strategies
WO2002014497A3 (en) Genes encoding mutant p-coumarate 3-hydroxylase (c3h) and methods of use
EP1634962A4 (en) Method of detecting target nucleotide sequence, detection target structure to be used in embodying the method, process for producing the same and assay kit for detecting target nucleotide sequence
WO2004070035A3 (en) Method for increasing efficiency of homologous recombination in plants

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP