WO2004070035A3 - Method for increasing efficiency of homologous recombination in plants - Google Patents

Method for increasing efficiency of homologous recombination in plants Download PDF

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Publication number
WO2004070035A3
WO2004070035A3 PCT/EP2004/000964 EP2004000964W WO2004070035A3 WO 2004070035 A3 WO2004070035 A3 WO 2004070035A3 EP 2004000964 W EP2004000964 W EP 2004000964W WO 2004070035 A3 WO2004070035 A3 WO 2004070035A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
homologous recombination
plants
caltractin
activity
Prior art date
Application number
PCT/EP2004/000964
Other languages
French (fr)
Other versions
WO2004070035A2 (en
Inventor
Rocio Sanchez-Fernandez
Barbara Hohn
Jean Molinier
Original Assignee
Sungene Gmbh & Co Kgaa
Rocio Sanchez-Fernandez
Barbara Hohn
Jean Molinier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungene Gmbh & Co Kgaa, Rocio Sanchez-Fernandez, Barbara Hohn, Jean Molinier filed Critical Sungene Gmbh & Co Kgaa
Publication of WO2004070035A2 publication Critical patent/WO2004070035A2/en
Publication of WO2004070035A3 publication Critical patent/WO2004070035A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to methods for increasing efficiency of homologous recombination in plants or plant cells, by decreasing expression or activity of at least one caltractinlike protein in said plant or plant cells. The method may be used for inserting sequences into a distinct locus of the chromosomal DNA. Furthermore the method may be used for deleting sequences from the chromosomal DNA flanked by homology sequences by initiating homologous recombination between said homology sequences. The invention furthermore relates to expression constructs suitable for-expressing at least part of a nucleic acid sequence encoding for a caltractin-like protein (e.g., in form of antisense or double-stranded RNA) suitable for decreasing expression or activity of at least one caltractin-­like protein in plant or plant cells. Object of the invention are furthermore transgenic plants comprising a decreased expression or activity of at least one caltractin-like protein and the use of said transgenic plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.
PCT/EP2004/000964 2003-02-03 2004-02-03 Method for increasing efficiency of homologous recombination in plants WO2004070035A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44456003P 2003-02-03 2003-02-03
US60/444,560 2003-02-03

Publications (2)

Publication Number Publication Date
WO2004070035A2 WO2004070035A2 (en) 2004-08-19
WO2004070035A3 true WO2004070035A3 (en) 2004-12-16

Family

ID=32850886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/000964 WO2004070035A2 (en) 2003-02-03 2004-02-03 Method for increasing efficiency of homologous recombination in plants

Country Status (1)

Country Link
WO (1) WO2004070035A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2371967B1 (en) 2005-03-18 2015-06-03 DSM IP Assets B.V. Production of carotenoids in oleaginous yeast and fungi
US9018013B2 (en) * 2011-08-12 2015-04-28 Life Technologies Corporation Molecular biology tools for algal engineering
EP2612918A1 (en) 2012-01-06 2013-07-10 BASF Plant Science Company GmbH In planta recombination
UA118090C2 (en) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
CN108345219B (en) * 2018-03-01 2020-01-17 东华大学 Polyamide fiber production process based on brain-like memory GRU

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002024890A2 (en) * 2000-09-18 2002-03-28 Pioneer Hi-Bred International, Inc. Rice mlh1 ortholog and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002024890A2 (en) * 2000-09-18 2002-03-28 Pioneer Hi-Bred International, Inc. Rice mlh1 ortholog and uses thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BHATTACHARYA D ET AL: "Molecular cloning and evolutionary analysis of the calcium-modulated contractile protein, centrin, in green algae and land plants", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 23, no. 1, 1993, pages 1243 - 1254, XP002266444, ISSN: 0167-4412 *
CORDEIRO M C R ET AL: "Characterization of early induced genes in Arabidopsis thaliana responding to bacterial inoculation: identification of centrin and of a novel protein with two regions related to kinase domains", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 434, no. 3, 4 September 1998 (1998-09-04), pages 387 - 393, XP004258807, ISSN: 0014-5793 *
MENGISTE T ET AL: "PROSPECTS FOR THE PRECISE ENGINEERING OF PLANT GENOMES BY HOMOLOGOUS RECOMBINATION", BIOLOGICAL CHEMISTRY, XX, XX, vol. 380, no. 7/8, 1999, pages 749 - 758, XP000885763, ISSN: 1431-6730 *
STOPPIN-MELLET VIRGINIE ET AL: "Characterization of microsome-associated tobacco BY-2 centrins", EUROPEAN JOURNAL OF CELL BIOLOGY, vol. 78, no. 11, November 1999 (1999-11-01), pages 842 - 848, XP009031813, ISSN: 0171-9335 *
VEERARAGHAVAN SUDHA ET AL: "Structural independence of the two EF-hand domains of caltractin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 32, 9 August 2002 (2002-08-09), pages 28564 - 28571, XP002283043, ISSN: 0021-9258 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
US9297031B2 (en) 2006-09-28 2016-03-29 Dsm Ip Assets B.V. Production of carotenoids in oleaginous yeast and fungi

Also Published As

Publication number Publication date
WO2004070035A2 (en) 2004-08-19

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