WO2004070035A3 - Method for increasing efficiency of homologous recombination in plants - Google Patents
Method for increasing efficiency of homologous recombination in plants Download PDFInfo
- Publication number
- WO2004070035A3 WO2004070035A3 PCT/EP2004/000964 EP2004000964W WO2004070035A3 WO 2004070035 A3 WO2004070035 A3 WO 2004070035A3 EP 2004000964 W EP2004000964 W EP 2004000964W WO 2004070035 A3 WO2004070035 A3 WO 2004070035A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- protein
- homologous recombination
- plants
- caltractin
- activity
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to methods for increasing efficiency of homologous recombination in plants or plant cells, by decreasing expression or activity of at least one caltractinlike protein in said plant or plant cells. The method may be used for inserting sequences into a distinct locus of the chromosomal DNA. Furthermore the method may be used for deleting sequences from the chromosomal DNA flanked by homology sequences by initiating homologous recombination between said homology sequences. The invention furthermore relates to expression constructs suitable for-expressing at least part of a nucleic acid sequence encoding for a caltractin-like protein (e.g., in form of antisense or double-stranded RNA) suitable for decreasing expression or activity of at least one caltractin-like protein in plant or plant cells. Object of the invention are furthermore transgenic plants comprising a decreased expression or activity of at least one caltractin-like protein and the use of said transgenic plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals, in particular for the production of oils.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US44456003P | 2003-02-03 | 2003-02-03 | |
US60/444,560 | 2003-02-03 |
Publications (2)
Publication Number | Publication Date |
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WO2004070035A2 WO2004070035A2 (en) | 2004-08-19 |
WO2004070035A3 true WO2004070035A3 (en) | 2004-12-16 |
Family
ID=32850886
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2004/000964 WO2004070035A2 (en) | 2003-02-03 | 2004-02-03 | Method for increasing efficiency of homologous recombination in plants |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2004070035A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8691555B2 (en) | 2006-09-28 | 2014-04-08 | Dsm Ip Assests B.V. | Production of carotenoids in oleaginous yeast and fungi |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2371967B1 (en) | 2005-03-18 | 2015-06-03 | DSM IP Assets B.V. | Production of carotenoids in oleaginous yeast and fungi |
US9018013B2 (en) * | 2011-08-12 | 2015-04-28 | Life Technologies Corporation | Molecular biology tools for algal engineering |
EP2612918A1 (en) | 2012-01-06 | 2013-07-10 | BASF Plant Science Company GmbH | In planta recombination |
UA118090C2 (en) * | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
CN108345219B (en) * | 2018-03-01 | 2020-01-17 | 东华大学 | Polyamide fiber production process based on brain-like memory GRU |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002024890A2 (en) * | 2000-09-18 | 2002-03-28 | Pioneer Hi-Bred International, Inc. | Rice mlh1 ortholog and uses thereof |
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2004
- 2004-02-03 WO PCT/EP2004/000964 patent/WO2004070035A2/en active Search and Examination
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002024890A2 (en) * | 2000-09-18 | 2002-03-28 | Pioneer Hi-Bred International, Inc. | Rice mlh1 ortholog and uses thereof |
Non-Patent Citations (5)
Title |
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BHATTACHARYA D ET AL: "Molecular cloning and evolutionary analysis of the calcium-modulated contractile protein, centrin, in green algae and land plants", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 23, no. 1, 1993, pages 1243 - 1254, XP002266444, ISSN: 0167-4412 * |
CORDEIRO M C R ET AL: "Characterization of early induced genes in Arabidopsis thaliana responding to bacterial inoculation: identification of centrin and of a novel protein with two regions related to kinase domains", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 434, no. 3, 4 September 1998 (1998-09-04), pages 387 - 393, XP004258807, ISSN: 0014-5793 * |
MENGISTE T ET AL: "PROSPECTS FOR THE PRECISE ENGINEERING OF PLANT GENOMES BY HOMOLOGOUS RECOMBINATION", BIOLOGICAL CHEMISTRY, XX, XX, vol. 380, no. 7/8, 1999, pages 749 - 758, XP000885763, ISSN: 1431-6730 * |
STOPPIN-MELLET VIRGINIE ET AL: "Characterization of microsome-associated tobacco BY-2 centrins", EUROPEAN JOURNAL OF CELL BIOLOGY, vol. 78, no. 11, November 1999 (1999-11-01), pages 842 - 848, XP009031813, ISSN: 0171-9335 * |
VEERARAGHAVAN SUDHA ET AL: "Structural independence of the two EF-hand domains of caltractin", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 277, no. 32, 9 August 2002 (2002-08-09), pages 28564 - 28571, XP002283043, ISSN: 0021-9258 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8691555B2 (en) | 2006-09-28 | 2014-04-08 | Dsm Ip Assests B.V. | Production of carotenoids in oleaginous yeast and fungi |
US9297031B2 (en) | 2006-09-28 | 2016-03-29 | Dsm Ip Assets B.V. | Production of carotenoids in oleaginous yeast and fungi |
Also Published As
Publication number | Publication date |
---|---|
WO2004070035A2 (en) | 2004-08-19 |
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