EP0912722A4 - Method of constructing vectors for homologous recombination directed mutagenesis - Google Patents

Method of constructing vectors for homologous recombination directed mutagenesis

Info

Publication number
EP0912722A4
EP0912722A4 EP98908603A EP98908603A EP0912722A4 EP 0912722 A4 EP0912722 A4 EP 0912722A4 EP 98908603 A EP98908603 A EP 98908603A EP 98908603 A EP98908603 A EP 98908603A EP 0912722 A4 EP0912722 A4 EP 0912722A4
Authority
EP
European Patent Office
Prior art keywords
homologous recombination
directed mutagenesis
constructing vectors
recombination directed
vectors
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98908603A
Other languages
German (de)
French (fr)
Other versions
EP0912722A1 (en
Inventor
Michael Nehls
Sigrid Wattler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP0912722A1 publication Critical patent/EP0912722A1/en
Publication of EP0912722A4 publication Critical patent/EP0912722A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP98908603A 1997-02-21 1998-02-20 Method of constructing vectors for homologous recombination directed mutagenesis Withdrawn EP0912722A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19707012 1997-02-21
DE19707012 1997-02-21
PCT/US1998/003243 WO1998037175A1 (en) 1997-02-21 1998-02-20 Method of constructing vectors for homologous recombination directed mutagenesis

Publications (2)

Publication Number Publication Date
EP0912722A1 EP0912722A1 (en) 1999-05-06
EP0912722A4 true EP0912722A4 (en) 2001-12-05

Family

ID=7821111

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98908603A Withdrawn EP0912722A4 (en) 1997-02-21 1998-02-20 Method of constructing vectors for homologous recombination directed mutagenesis

Country Status (3)

Country Link
EP (1) EP0912722A4 (en)
JP (1) JP2002514072A (en)
WO (1) WO1998037175A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU777909B2 (en) 1999-01-19 2004-11-04 Lexicon Genetics Incorporated Mammalian cortexin-like proteins and polynucleotides encoding the same
WO2000055311A2 (en) * 1999-03-17 2000-09-21 E.I. Du Pont De Nemours And Company Gene modification by homologous recombination
US6548271B1 (en) 1999-04-22 2003-04-15 Lexicon Genetics Incorporated Nucleic acids encoding human transporter proteins
EP1222269A1 (en) 1999-10-12 2002-07-17 Lexicon Genetics Incorporated Human ldl receptor family proteins and polynucleotides encoding the same
DE10016523A1 (en) * 2000-04-03 2001-10-04 Ingenium Pharmaceuticals Ag Cloning system for the construction of homologous recombination vectors
US20020031829A1 (en) * 2000-08-15 2002-03-14 Brian Zambrowicz Arrayed collection of genomic clones
SG164274A1 (en) * 2000-08-25 2010-09-29 Pfizer Prod Inc Rapid creation of gene targeting vectors using homologous recombination in yeast
US6376192B1 (en) 2000-11-28 2002-04-23 Baylor College Of Medicine Method for screening of DNA libraries and generation of recombinant DNA constructs
DE10133928A1 (en) * 2001-07-12 2003-01-23 Bayer Cropscience Ag Preparation of deletional mutants, useful for high-throughput screening to identify essential genes, potential targets for biologically active agents, comprises a polymerase chain reaction

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014769A1 (en) * 1993-11-29 1995-06-01 University Of South Florida TRANSGENIC MOUSE PREPARED USING YEAST ARTIFICIAL CHROMOSOMES (YACs) AND HOMOLOGOUS RECOMBINATION

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995014769A1 (en) * 1993-11-29 1995-06-01 University Of South Florida TRANSGENIC MOUSE PREPARED USING YEAST ARTIFICIAL CHROMOSOMES (YACs) AND HOMOLOGOUS RECOMBINATION

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
NEHLS M ET AL: "Two large insert vectors, lambda PS and lambda KO, facilitate rapid mapping and targeted disruption of mammalian genes", BIOTECHNIQUES, vol. 17, no. 4, October 1994 (1994-10-01), pages 770 - 775, XP002179274 *
See also references of WO9837175A1 *
STORCK T ET AL: "Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouse", NUCLEIC ACIDS RESEARCH., vol. 24, no. 22, 15 November 1996 (1996-11-15), pages 4594 - 4596, XP002076777 *
TSUZUKI T ET AL: "Embryonic stem cell gene targeting using bacteriophage lambda vectors generated by phage-plasmid recombination", NUCLEIC ACIDS RESEARCH., vol. 26, no. 4, 15 February 1998 (1998-02-15), pages 988 - 993, XP002179275 *
WATTLER S ET AL: "Construction of gene targeting vectors from lambda KOS genomic libraries", BIOTECHNIQUES, vol. 26, no. 6, June 1999 (1999-06-01), pages 1150-1156, 1158, 1160, XP002179272 *

Also Published As

Publication number Publication date
WO1998037175A1 (en) 1998-08-27
EP0912722A1 (en) 1999-05-06
JP2002514072A (en) 2002-05-14

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