WO1998024459A1

WO1998024459A1 - Substance in the form of an aqueous extract of vegetal raw material for treating oncological diseases, and method for producing the same

Info

Publication number: WO1998024459A1
Application number: PCT/RU1997/000391
Authority: WO
Inventors: Georgy Nikolaevich Vorozhtsov; Elena Jurievna Vinokurova; Natalya Ivanova Kazachkina; Jury Mikhailovich Luzhkov; Elena Romanovna Nemtsova; Tatyana Nikolaevna Petrova; Genrikh Alexandrovich Tolstikov; Valery Ivanovich Chissov; Elvira Eduardovna Shults; Raisa Ivanovna Yakubovskaya
Original assignee: Gosudarstvenny Nauchny Tsentr Rossiiskoi Federatsii 'niopik' (Gnts Rf 'niopik'); Novosibirsky Institut Organischeskoi Khimii Sibirskogo Otdelenia Rossiiskoi Akademii Nauk; Moskovsky Naucho Issledovatelsky Onkologichesky Institut Im P.A.Gertsena (Mnioi Im P.A.Gertsena)
Priority date: 1996-12-04
Filing date: 1997-12-03
Publication date: 1998-06-11
Also published as: RU2118536C1

Abstract

The present invention relates to a substance in the form of an aqueous extract of vegetal raw material which is used for treating oncological diseases, as well as to method for producing said substance. The substance comprises the aqueous extracts of Calendula officinalis, Plantago lanceolata, Pentaphilloides fruticosa and Angelica sylvestris, wherein said extracts are obtained from the following amounts in grams of said plants and water: from 200 to 360 of fresh Calendula officinalis; from 10 to 60 of dried Plantago lanceolata; from 10 to 60 of dried Pentaphilloides fruticosa; from 25 to 60 of dried Angelica sylvestris; and 3000 grams of water. The method for producing said aqueous extracts comprises macerating the plants in water before press-filtering the mixture and submitting it to a centrifugal treatment. This substance may be used in clinical oncology as part of a program for treating oncological diseases.

Description

ΡСΤ / ΙШ97 / 00391

Medicament for the treatment of oncologic diseases in the form of an aqueous extract of a process of raw material and its production method

The invention is supplied to medicine, in particular, to phytotherapy and is related to the treatment of ecologically harmful diseases and food

A simple product is known, which includes the growth of plant growth, as well as sulfuric acid and sulfuric acid, which are also excreted in sulfuric acid. [1]

This is a low-cost product that can be used only for outdoor use.

Izvesτen sοsτav, ρeκοmendοvanny in κachesτve vsποmοgaτelnοgο sρedsτva, ποvyshayuschegο προτivοοπuχοlevuyu aκτivnοsτ, vκlyuchayu- conductive among προchiχ vοdnyχ (or vοdnο-ορganichesκiχ) eκsτρaκτοv ρasτeny eκsτρaκτy Αδϊgaβaϊiδ, Ρasοηϊa, Αη§eΗsa [2].

A large number of commercially available components has been disclosed, including as part of the growth of plants for the production of food and food [3].

The disadvantages of these components are their many components.

The objective of this invention was to investigate a medication for the treatment of ecologic diseases in the form of extrudates that are available for sale in the market.

For this task ρesheniya πρedlοzhenο sρedsτvο, sοdeρzhaschee vοdnye eκsτρaκτy ρasτeny Saϊeηάiϊa οgBstaΗδ (fresh) Ρϊaηιaβο Ιaηseοϊaιa (suχοe) ΡeηιaρShοϊάeδ ϊltsοδa (suχοe) and ΑηveΗsa δuϊνeδϊήδ (suχοe) ποluchennye πρi following sοοτnοshenii ρasτeny and vοdy, r: Saϊeηάiϊa οgBsϊηaIδ fresh - 200- 360 ΡΙаηШβο Ιаηсеοϊааа sukhoi - 10-60

ΗеηιаρЬШОϊάеδ ϊχиύсоза Dry - 10-60;

Даοда - 3000

The use of specific components is offered in quantities smaller than the lower limit of the indicated range of values, which reduces the therapeutic effect; The outside of the scope of the indicated diameters is limited by the volume of water necessary for the receipt of the external extracts. \ УΟ 98/24459

2

The process of production of plants was known through the processing of their water or an external chemical source [4].

Also known is the method of production of the product from the industrial waste, including the extraction of the crushed grass by soaking (maceration), emulsification and irrigation.

This method is provided by technologically sophisticated, t.k. There is a two-stage maceration.

The objective of the proposed invention was the development of a therapeutic method of obtaining an external treatment for the treatment of diseases of the patient.

To solve this problem, it is necessary to soak the plants (in the form of plants, leaves or roots) in the water with the next cessation and centrifugation.

The biological activity of plant-derived active substances was controlled by the division of antioxidant and chronic virulent activity.

Antioxidant activity of food products was shared by the process of inhibition of the sample of the process of the reduction of the food of the liver (liver).

The cytotoxic activity of ϊη vuga was shared with the use of biology, which was based on the inhibition of the disease

Evaluating the beneficial activity of the drugs was applied to the mouse with leukemia using different doses of the drugs. Estimates were served by: a decrease in the occupancy rate (ΤΡΟ) and an increase in the longevity of life of living (VL), the average longevity of life (LN). In addition, we evaluated the effect of ectropacts on the therapeutic activity of cystic acid diamine platinum (DDP) [8].

The following examples illustrate the proposed invention.

For example, 1.

Beρuτ shredded fresh lisτya Saϊeηάiϊa οShsϊηaΗz (ZΟΟg) suχie lisτya Ρϊaηιaβο Ιaηseοϊaιa (25d) ποbegy ΡeηιaρShΙοϊάeδ ychϊιyuοza (25d) and κορni Αη ^ eΗsa zuϊνezιήz (60 g), ποmeschayuτ in sτeκlyanny or emaliροvannιy geρmeτichesκi zaκρyvayuschiysya sοsud, zalivayuτ 3L vοdy and οsτavlyayuτ at a temperature of 28 ° С for 8 days, at a temperature and stirring. The obtained product is refined and centered (20 ° С, 3000 rpm, 40 min.). The garden is absorbed, and the filter is used. \ ΥΟ 98/24459

EXAMPLES 2 - 7 PLANT EXTRACTS PRODUCED BY METHOD. described in Example 1, but they change the quantity of the mixture and the conditions of maceration. The compositions are listed in table 1.

Table 1

\ U

Separation of antioxidant activity of plant extracts.

Antioxidant activity was studied using the method, based on the methods of the studied inhibition of the process of inactivity is inactive (inactive) The liver of mice (СΒΑ.ΒϋΡ-1 lines was harmless) was homogenized in a glass homogenized with the following centrifugation. The liver of the liver was introduced from the calculation of 5-6 mg of protein per sample. The volume of the product was increased to 0.5 ml of the product tested for the antioxidant activity of the sample. Were incubated in the course of Zch. πρ and 37 ° С. The concentration of PEL products was shared with the color reaction with the acid. For a unit of activity of the studied sample, we took quantitative antioxidants. Necessary for 50% inhibition of PUL.

The results are presented in table 2.

Table 2 Comparison of antioxidant activity of samples of vegetable drugs with known antioxidant drugs.

As can be seen from Table 2, the cultivars 1.2 and II have a significant anti-oxidative activity in the test η ϊϊ,, Increasing the rate of known well-known anti-vitamin vitamins (C and β-carotene).

Table 2a Change in the antioxidant activity of the samples of the plant's industrial waste products.

Separation of cytotoxic activity of plants

Pρi ποsτanοvκe tsiτοτοκsichesκοgο biοτesτa ϊη νϊϊgο in lunκi πlοsκοdοnnοgο 96 lunοchnοgο miκροπlansheτa (δagzϊeάϊ, SSHΑ) vnοsili πο 100 mκl susπenzy κleτοκ Α-549 line κοntsenτρatsii Ι χΙΟ κleτοκ ⁵ / ml 24 h dοbavleniem cheρez ρazvedeniya οbρaztsοv ρasτiτelnyχ eκsτρaκτοv (2, 2 mg / ^' ml) in a volume of 100 μl / well. On the other hand, the internal laboratory used DDP in the range of concentration of 40-1.25 mcg / ml in the final dilution. The statistical activity of the samples of the extracts and the DDP was evaluated by the test method [7].

The inhibition of the polishing of cells> τ-549 more than 50 ° али was considered to be biologically significant.

The results are shown in table 3.

Table 3 The net activity of plant growth inhibition of lung cell carcinoma by them inactivating the lungs of a human carcinoma 54.

b

Issledοvannys οbρaztsy ρasτiτelnyχ eκsτρaκτοv οbladaκπ The cylinder τοτοκsichesκοy aκτivnοsτyο in eκsπeρimenτaχ ϊη νϊϊgο, οdnaκο οna Su- schesτvennο below τaκοvοy πρeπaρaτa PDD τρaditsiοnnο isποlzuemοgο κaκ κοnτροlny πρeπaρaτ in τesτe ingibiροvaniya προliφeρatsii ϊη νϊϊgο (eκsτρaκτ - 2.2 mg / ml, DCF - 0.01 mg / ml provides 70-80% inhibition)

The study of the susceptibility of samples of vegetative plant extracts.

The study of transferability and harmlessness of samples was carried out on live with the b-1210, it is convenient. Samples of the extracts were introduced into the body through a large number of samples during the whole life of animals, starting from the beginning of the day with a dose of 10.8 ml / day. Commercial mice received 0.9% of the growth of the environment, along with the growth of the extracts.

The physical effect was evaluated by the change in body mass of mice. as well as their appearance (introduction, appearance of quick disks, and their motor activity). For a quantitative assessment of changes in motor activity, we entered the integral index: (a) χ100%, where the total number of mice with a reduced motor is L is the total number of observations from the 1st to the 6th days of the growth of the weather ^, (1 observation-1 live mouse per day).

The results of the above experiments are shown. that plant extracts in a separate dose of 10.8 ml'kg do not affect the body mass, performance. view of the long arm and the mobility of mice (Fig. 1).

Increasing ρazοvοy dοzy ρasτiτelnyχ eκsτρaκτοv dο 1 ml (maκsimalnο dοπusτimy οbem) πρi οdnοκρaτnοm vnuτρibρyushin- nοm πρeπaρaτοv administered 1, 2, 3 is not πρivοdilο κ ρazviτiyu vidimyχ naρusheny in ποvedenii and appearance zhivοτnyχ not vyzyvalο iχ death chτο uκazyvaeτ on οτsuτsτvie vοzmοzhnοsτi were οπρedeleniya - the rank of LDU, LD50, LDιοο for these plant extracts.

Separation of the productive activity of the samples of the plant active substances.

Limφοidnuyu leyκemiyu Y210 πρivivali male mice ΒBΡι sτeρilnοm φiziοlοgichesκοm ρasτvορe πο ZχΙΟ ⁶ κleτοκ οbeme in 0.2 l. The holiday was considered a zero day of business. Samples were delivered in doses and modes indicated in tables 4 and 5.

The treatment of animals started after 24 hours after getting upset, but the live animals received a physiological \ disaster along with test samples of extracts. The net ef- fect was evaluated for the rate of growth of the mean (ΤΡΟ) calculated by the formula:

ΤΡΟ = [(νκοntτ-νοπьη) / νκοττ] χ! 00%; the average life expectancy (LIF), the increase in life expectancy (LIF), calculated by the formula:

UPZ = [(SPZhopyt-SPZhkontp) / SPZhkontp] χ 100%.

The data obtained were processed by the method of the Student with the use of statistical programs. The differences were considered valid πρ and ρ <0.05.

The results of the operation of the vegetable products are presented in Table 4, from which it is clearly visible that the products are capable of suppressing the process.

5 Table 5 shows the results of the influence of plant extracts on the therapeutic effect of DDP. As can be seen from Table 5, the effects increase the therapeutic effect of the statistics, due to the increase in the value of ΤΡΟ by the 6th, 8th day of the discharge.

Table 4 Influence of samples of plant growth products on the L-1210.

Table 5 Influence of samples of plant extracts on the therapeutic effect of DDP.

As can be seen from Tables 2 - 5 and the picture of the preparation, the expansive device indicates a self-contained negative effect, delaying 12 pct; does not reduce the therapeutic effect of DDP and reduces the toxic effect of the last. In general, this system may be ineffective for use in a clinical oncology to include in the treatment of an oncologic patient.

Claims

5 ΡСΤ / ΚШ7 / 00391

Formula of the invention

Ι .Sρedsτvο for treating οnκοlοgichesκiχ zabοlevany as vοdnοgο eκsτρaκτa ρasτiτelnοgο syρya, οτlichayuscheesya τem, chτο οnο vκlyuchaeτ eκsτρaκτy ρasτeny Saϊeηάiϊa οShsϊηaΗδ, Ρ1aηϊa§ο Ιaηseοϊaϊa, ΡeηϊaρShοϊάez & iϊyuο8a and ΑηoeΗsa zuϊνeδϊπδ ποluchennye πρi following present sοοτnοshenii ρasτeny and vοdy, g:

Sϊеηάиϊа οШсϊηаΗз fresh - 200-360

Ϊаηϊаβο Ιаηсеοϊаϊа СУХОЕ - 10-60

ΪеηϊаρЬШοιάез ϋгиϊюοδа СУХОе - 10-60

ΑηβеΗsa δуϊνезϊηз suχοе - 25-60

Даοда - 3000

2. Sποsοb πρigοτοvleniya sρedsτva for treating οnκοlοgichesκiχ zabοlevany as vοdnοgο eκsτρaκτa ρasτeny, οτlichayuschiysya τem, chτο vymachivayuτ in vοde ρasτeniya Saϊeηάiϊa οShsϊηaΗz, Ρ1aηϊa§ο Ιaηseο- Ιaϊa, ΡeηϊaρShοϊάeδ ιgiϊϊsοza and ΑηβeΗsa δuϊνezϊήδ with ποsleduyuschim προ- tsezhivaniem and tsenτρiφugiροvaniem.

Sources of information that should be taken into account when preparing the descriptions:

[1] Patent of the Company 2502957, Α61Κ35 / 78, 1982 [2] Patent of the Company 2531864, Α61Κ35 / 78, 1983 [3] Patent of the United Kingdom 2226494, Α61Κ35 / 78, 1990 [4] Application no. 2561359, / 78, 1983 [5] Patent ΡΦ 2036655, Α61Κ35 / 78, 1995.

[6] Steel ID, Garitvili Τ.Τ. "Modern methods in biology." Publishing House "Medicine", Russia, 1977, p. 66-68 [7] Μοзтаηη ΤΚ, Ροηё Τ.Α.Τ. ρ ϊ с с с а δ δ I. I. I. I. I. I. I. I. I. I. I., 1983 1983 1983 1983, 1983, ν.65

[8] Schellen L., Ekkard Sh., Ееeleeb Α. "Experimental evaluation of drugs in the USSR and the USA", Publishing House "ρиρ", Moscow, 1980, p. 270-274