WO1998017992A3 - Techniques de formation d'images d'hybridation in situ par fluorescence multicolore (m-fish) utilisant de nombreux filtres multibandes avec superposition d'images - Google Patents

Techniques de formation d'images d'hybridation in situ par fluorescence multicolore (m-fish) utilisant de nombreux filtres multibandes avec superposition d'images Download PDF

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Publication number
WO1998017992A3
WO1998017992A3 PCT/US1997/019464 US9719464W WO9817992A3 WO 1998017992 A3 WO1998017992 A3 WO 1998017992A3 US 9719464 W US9719464 W US 9719464W WO 9817992 A3 WO9817992 A3 WO 9817992A3
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WO
WIPO (PCT)
Prior art keywords
images
image
fish
cubes
multifluor
Prior art date
Application number
PCT/US1997/019464
Other languages
English (en)
Other versions
WO1998017992A2 (fr
Inventor
Jacques Ginestet
Original Assignee
Applied Imaging Inc
Jacques Ginestet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/901,543 external-priority patent/US5880473A/en
Application filed by Applied Imaging Inc, Jacques Ginestet filed Critical Applied Imaging Inc
Priority to AU49202/97A priority Critical patent/AU4920297A/en
Publication of WO1998017992A2 publication Critical patent/WO1998017992A2/fr
Publication of WO1998017992A3 publication Critical patent/WO1998017992A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Abstract

Cette invention se rapporte à des techniques d'acquisition et d'analyse d'images d'hybridation in situ par fluorescence multicolore (M-FISH) qui permettent un alignement correct des images, ainsi qu'à des techniques de combinaison d'une pluralité d'images étiquetées en vue de la formation d'images marquées. Des illuminateurs verticaux utilisent de multiples cubes filtrants pour différents sous-ensembles de l'ensemble total des colorants désirés. On résout le problème d'alignement en configurant les différents cubes multibandes de façon à ce que des paires de cubes soient configurées pour un colorant commun. Conformément à l'invention, un premier jeu d'images générées avec un premier cube inclut une image qui correspond à (ou contient les mêmes caractéristiques que) l'une des images d'un second ensemble d'images générées avec un second cube. Ceci permet d'aligner de manière précise et exacte le premier jeu d'images avec le second jeu d'images. Un procédé de génération d'une image étiquetée consiste spécifiquement en une normalisation des intensités des pixels à l'intérieur de chaque image, suivie d'une transformation en images-ratios.
PCT/US1997/019464 1996-10-25 1997-10-17 Techniques de formation d'images d'hybridation in situ par fluorescence multicolore (m-fish) utilisant de nombreux filtres multibandes avec superposition d'images WO1998017992A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU49202/97A AU4920297A (en) 1996-10-25 1997-10-17 Multifluor-fluorescence in situ hybridization (m-fish) imaging techniques using multiple multiband filters with image registration

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US2931496P 1996-10-25 1996-10-25
US60/029,314 1996-10-25
US08/901,543 1997-07-28
US08/901,543 US5880473A (en) 1997-07-28 1997-07-28 Multifluor-fluorescence in-situ hybridization (M-FISH) imaging techniques using multiple multiband filters with image registration

Publications (2)

Publication Number Publication Date
WO1998017992A2 WO1998017992A2 (fr) 1998-04-30
WO1998017992A3 true WO1998017992A3 (fr) 1998-07-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1997/019464 WO1998017992A2 (fr) 1996-10-25 1997-10-17 Techniques de formation d'images d'hybridation in situ par fluorescence multicolore (m-fish) utilisant de nombreux filtres multibandes avec superposition d'images

Country Status (3)

Country Link
US (1) US6225636B1 (fr)
AU (1) AU4920297A (fr)
WO (1) WO1998017992A2 (fr)

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Publication number Publication date
US6225636B1 (en) 2001-05-01
WO1998017992A2 (fr) 1998-04-30
AU4920297A (en) 1998-05-15

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