WO1998004725A1 - Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues - Google Patents
Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues Download PDFInfo
- Publication number
- WO1998004725A1 WO1998004725A1 PCT/US1997/013358 US9713358W WO9804725A1 WO 1998004725 A1 WO1998004725 A1 WO 1998004725A1 US 9713358 W US9713358 W US 9713358W WO 9804725 A1 WO9804725 A1 WO 9804725A1
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- WIPO (PCT)
- Prior art keywords
- plant
- altered
- amount
- dna
- methylated dna
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1007—Methyltransferases (general) (2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Definitions
- Plant genomes contain relatively large amounts of the modified nucleotide
- ddm mutants exhibit a slight delay (1.7 days) in flowering, altered
- a reduction in the rate a plant matures can be used to increase the biomass
- the present invention is based on the unexpected observation that a decrease
- DNA present in a plant genome results in a plant that requires less time to mature.
- the rate of maturation is increased by altering the plant
- plant cells plant tissues or plant seeds, using molecular techniques, such that the plant
- the rate of maturation is decreased by altering a plant, plant cells, plant tissues or plant seeds, using molecular
- the present invention further provides plants that have an altered rate of
- Figure 1 The predicted MET1 gene product and antisense construct.
- the METJ protein is a 1,534-amino acid protein with a high degree
- MET! antisense construct is shown in the bottom of the figure. See Example 1.
- mutants were digested with Hpa II (left panels) or Msp I (right panels), subjected to
- LEAFY LFY
- Tr246 to wild-type Arabidopsis Cold-type Arabidopsis (Columbia strain with no mutations) were digested
- the present invention is based on the unexpected observation that DNA
- methylation particularly at cytosine nucleotides, is involved in regulating the rate of
- the present invention is based on the
- the present invention provides methods for
- maturation refers to the process of plant differentiation leading
- the method will produce plants with an increased
- Arabidopsis plants were obtained which developed flowers after 10 to 12 days.
- the result of accelerating plant development is the ability to decrease the
- biomass refers to the total plant weight, particularly leafy material. The amount of leaf material is increased in plants altered
- Another consequence of delaying flower production is to produce plants that
- plants propagated containing the genetic alteration can be used in the present method. Methods known in the art for molecularly altering a plant are discussed in detail
- the preferred plants include both dicot and monocotyledonous plants.
- most preferred plants are plants with economic value as a food or biomass source, or
- plants with long maturation times Such plants include, but are not limited to, leafy
- plants such as tobacco, spinach, lettuce, and seed bearing plants such as rice, corn, soy
- the methods of the present invention rely on altering plants using molecular
- non-molecular methods namely selecting plants generated through methods such as
- plants and plant cells can be subjected to chemical mutagenesis, physical mutagenesis
- Plants can then be
- the methods of the present invention can be used to alter
- a plant is said to have a reduced or decreased amount of
- methylated DNA when the plant has less methylated DNA than the non-altered plant.
- cytosine has been seen to be methylated in plants.
- present methods are therefore accomplished by targeting the methylation of cytosine.
- cytosine were obtained.
- targets, strategies and molecular techniques can be used by a variety of targets, strategies and molecular techniques.
- methyl transferases that are normally produced in the plant.
- the methyl transferase can be altered in a variety of ways by a skilled artisan
- Any of a plant's DNA methyl transferases genes can be used as a target in the
- the most preferred targets are the genes encoding a cytosine methyl
- DNA methyl transferase genes examples include
- cytosine methyltransferase such as the MET1 Arabidopsis gene herein used, the
- plant is to create knockout mutants in the plant in which one or more of the plant's
- DNA methyl transferase genes are inactivated. This can be accomplished through the
- transposon mediated mutagenesis can be used for the same purpose.
- transferase gene allowing one to activate or deactivate methyl transferase expression
- the methods of the present invention can be used to alter
- a plant is said to have a reduced or decreased amount of
- methylated DNA when the plant has less methylated DNA than the non-altered plant.
- cytosine has been seen to be methylated in plants.
- present methods are therefore accomplished by targeting the methylation of cytosine.
- methyl transferase in the plant cell.
- the methyl transferase can be altered in a variety of ways by a
- DNA methyl transferase encoding expression units can be any suitable DNA methyl transferase encoding expression units.
- DNA methyl transferase encoding expression units can be any suitable DNA methyl transferase encoding expression units.
- expression units can be controlled either by a constitutive promoter, providing
- Any DNA methyl transferase gene, or active fragment thereof, can be used to generate a stable copy of a gene.
- DNA can be further altered so as to contain an expression unit that expresses a DNA
- plants are of additional value because of the ability to induce expression of a DNA
- the present invention further provides plants that have been altered using
- the plants of the present invention fall within two types.
- the first type is a first type
- plants of the present invention that have a slower rate of maturation will mature at a
- plants or the present invention include those that have been altered, using
- the preferred plants are plants for which it is desirable to reduce generation times for breeding or seed production
- expression units or expression vectors or systems to express an exogenously
- a supplied gene such as a DNA methyl transferase, or antisense molecule, in a plant.
- methylated DNA present in a plant cell typically employ a protein or
- antisense coding region such as the METl Arabidopsis gene, or a homologue thereof
- antisense coding region can either be the expression control element that is
- Transcription initiation regions can include any of the various opine
- initiation regions such as octopine, mannopine, nopaline and the like that are found in
- plant promoters such as prolifera promoter,
- promoters etc. can also be used. The most preferred promoters will be active in
- tissue particularly meristematic cells.
- Either a constitutive promoter such as the CaMV or Nos promoter illustrated above, an organ-specific promoter (such as the E8 promoter from tomato) or an
- inducible promoter is typically ligated to the protein or antisense encoding region
- the expression unit may be further
- the expression units will typically contain, in
- restriction enzyme sites at the 5' and 3' ends of the expression unit are typically
- the promoter is
- the expression cassette can also contain a promoter sequence
- the termination region may be obtained from the same gene as
- the promoter sequence or may be obtained from different genes. If the mRNA
- Polyadenylation sequences include, but are not limited to the Agrobacterium octopine
- the resulting expression unit is ligated into or otherwise constructed to be included in a vector which is appropriate for higher plant transformation.
- the vector will also typically contain a selectable marker gene by which transformed plant cells
- the marker gene can be identified in culture.
- the marker gene will encode antibiotic
- markers include resistance to G418, hygromycin, bleomycin,
- vector will be identified by their ability to grow on a medium containing the particular
- Replication sequences of bacterial or viral origin, are generally also
- a vector included to allow the vector to be cloned in a bacterial or phage host, preferably a
- bacteria should also be included to allow selection of bacterial cells bearing the
- Suitable prokaryotic selectable markers also include resistance to
- antibiotics such as kanamycin or tetracycline.
- T-DNA sequences will also be included for subsequent transfer to
- plants having an altered amount of methylated DNA can be produced by:
- a plant may also be used in the present methods. For example, formation of a triple
- photocrosslinking is described, e.g., in D. Praseuth, et al, Proc. Nat 'I Acad. Sci. USA
- transgenic plants are prepared which contain the desired expression unit or into which
- the vector is microinjected directly into plant cells by use of
- micropipettes to mechanically transfer the recombinant DNA into the plant cell
- nucleic acid either within the matrix of small beads or particles, or on the
- These entities are minicells, cells, lysosomes or other fusible lipid-surfaced
- DNA may also be introduced into the plant cells by electroporation (From et
- the cell wall divide, and regenerate.
- a plant cell For transformation mediated by bacterial infection, a plant cell is infected with
- Agrobacterium is a representative genus of the gram-negative family
- Rhizobiaceae Its species are responsible for crown gall (A. tumefaciens) and hairy
- the bacterial genes responsible for expression of opines are a
- assaying for the presence of opines can be used to identify transformed tissue.
- Heterologous genetic sequences can be introduced into appropriate plant cells,
- Ti or Ri plasmid is transmitted to plant cells on infection by Agrobacterium and is
- Ti and Ri plasmids contain two regions essential for the production of transformed
- T-DNA transferred DNA
- the other termed the virulence (yir) region, is
- the T-DNA will be any substance that is essential for the transfer of the T-DNA but is not itself transferred.
- the T-DNA will be any substance that is not be transferred.
- the transferred DNA region can be transferred into a plant cell even if the yji region is on a different plasmid (Hoekema, et al, Nature 303: 179-189 (1983)).
- the transferred DNA region can be
- plasmids and sometimes constructed from foreign sequences. These may include but
- co-integrate the shuttle vector containing the gene of
- the gene of interest is inserted into a shuttle vector containing the cz ' -v-acting elements required for plant transformation.
- the other necessary functions are
- the second method requires (a) that the intact plant tissues, such as
- cotyledons can be transformed by Agrobacterium and (b) that the transformed cells
- Plant cells which have been transformed can also be regenerated using known
- Means for regeneration vary from species to species of plants, but generally a suspension of transformed protoplasts or a petri plate containing transformed explants
- Callus tissue is formed and shoots may be induced from callus and
- somatic embryo formation can be induced in the
- the culture media will generally contain various amino acids and plant hormones, such as auxin and cytokinins. It is also advantageous to add glutamic acid and proline
- silencing inactivation of transgenes is known as silencing.
- T-DNA vector pMON530 (Monsanto) in the antisense orientation to the CaMV 35S
- T-DNA confers resistance to kanamycin (50 ⁇ g/mi) on transgenic plants.
- PROLIFERA PRL
- CONSTITUTIVELY PHOTOMORPHOGENIC 1 CO
- ERECTA ER
- Tr246 to wild-type Arabidopsis Cold-type Arabidopsis (Columbia strain with no mutations) were digested
- the METl gene represents one member of a
- METl gene is expressed in seedling, vegetative, and floral tissues; in the
- T-DNA single-locus transferred DNA
- g/1 locus as a marker (but otherwise wild type), except as noted by Agrobacterium-
- the plants used in this study represent the kanamycin-resistant progeny of TI outcrosses from single-locus lines Tr242, 243, 244, 245, and 248.
- Seedlings were transplanted into soil 8 days after germination.
- Genomic DNAs were digested with the isochizomers Hpa II or Msp I (Fig.
- Msp I can cleave C 5m CGC but not 5m CCGG (M. Nelson, et ⁇ l, Nucleic
- genomic DNAs were isolated from plants immediately after the onset of flowering,
- Spherical Microsorb C18 column (100 A pore size, 4.5 mm inner diameter by 15 cm length) with a 20 min. isocratic gradient of 2.5% methanol, 50 mM KH,PO 5 (pH 4.0),
- Tr248 was reduced 71% relative to wild-type levels; the weak line Tr245 showed a
- antisense lines was determined by reversed-phase HPLC using the method of Gehrke
- Tr248 1.83 ⁇ 0.16 28.7 71.3 ddml 1.60 ⁇ 0.04 25.0 75.0
- Flowering time refers to the number of days elapsed from seed germination until emergence of an inflorescence bolt 0.5 to 1.0 cm
- leaf number refers to the number of vegetative leaves initiated before
- Tr242 24.5 ⁇ 2.1 10.0 ⁇ 1.4 4.5 ⁇ 1.1
- Tr248 46.3 ⁇ 4.7 32.9 ⁇ 2.7 20.5 ⁇ 2.0
- Tr242, 243, 244, 245, and 248 were plated on MS
- Tr244, Tr246 and Tr248 plants initiated 30 to 35 vegetative nodes with delayed abaxial trichome production and flowered after 45 to 48 days
- vegetative rosettes including enhanced spiral phyllotaxy of 10 to 20 vegetative-like
- tertiary inflorescences showed gross abnormalities, including a threefold increase in
- MTase differs markedly from the phenotype produced by the treatment of Arabidopsis
- 5-AzaC produces early flowering in some late-flowering strains and mutants of Arabidopsis (J. E. Burn, et al, Proc.
- Methylation may serve as a primary signal to restrict meristem determinacy, or it may
- phase transitions are often irregular in strong antisense plan — the location of
- branches apical to flowers in the inflorescence transition zone may represent an
- plants were altered to contain an expression unit that expresses cytosine methyl transferase under the control of the CaMV 355 promoter or another plant promoter.
- the expression units were introduced into a plant using Agrobacterium as
- plants having an increased amount of methylated DNA produced flowers at 10 to 12
- the present methods can be applied to any plant that can be altered using
- the first step in applying the present methods is to select as
- the plant is altered so as to contain an increased amount of methylated DNA while to
- the second step may be to decide which method to employ to alter the plant.
- the amount of DNA methylation can be decreased by inactivating
- methylation can be increased by activating or increasing the methylase activity within
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Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97938066A EP0935666A1 (fr) | 1996-07-31 | 1997-07-30 | Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues |
CA002262779A CA2262779A1 (fr) | 1996-07-31 | 1997-07-30 | Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues |
AU40480/97A AU730644B2 (en) | 1996-07-31 | 1997-07-30 | Methods for altering the rate of plant development and plants obtained therefrom |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US2331496P | 1996-07-31 | 1996-07-31 | |
US60/023,314 | 1996-07-31 | ||
US08/902,902 US6011200A (en) | 1997-07-30 | 1997-07-30 | Methods for altering the rate of plant development and plants obtained therefrom |
Publications (1)
Publication Number | Publication Date |
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WO1998004725A1 true WO1998004725A1 (fr) | 1998-02-05 |
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Application Number | Title | Priority Date | Filing Date |
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PCT/US1997/013358 WO1998004725A1 (fr) | 1996-07-31 | 1997-07-30 | Procedes de modification de la vitesse de croissance de plantes et plantes ainsi obtenues |
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WO (1) | WO1998004725A1 (fr) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999055891A1 (fr) * | 1998-04-30 | 1999-11-04 | Washington University | Gene vegetal regulant la methylation de l'adn |
WO2001000801A2 (fr) * | 1999-06-23 | 2001-01-04 | Syngenta Participations Ag | Gene intervenant dans l'inhibition epigenetique d'un gene |
WO2001009299A2 (fr) * | 1999-07-30 | 2001-02-08 | University Of Bath | Plantes modifiees |
EP1082417A1 (fr) * | 1998-06-04 | 2001-03-14 | The University Of Queensland | Sequences genetiques modifiant le phenotype |
US7125857B2 (en) | 1997-08-29 | 2006-10-24 | The Regents Of The University Of California | Modulators of DNA cytosine-5 methyltransferase and methods for use thereof |
WO2005038040A3 (fr) * | 2003-10-14 | 2006-11-09 | Ceres Inc | Procedes et compositions permettant de modifier les phenotypes des semences |
US7429692B2 (en) | 2004-10-14 | 2008-09-30 | Ceres, Inc. | Sucrose synthase 3 promoter from rice and uses thereof |
Citations (2)
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WO1996014414A1 (fr) * | 1994-11-02 | 1996-05-17 | John Innes Centre Innovations Limited | Regulation genetique du fleurissement |
DE4444460A1 (de) * | 1994-11-29 | 1996-05-30 | Inst Genbiologische Forschung | Verfahren zur Steigerung des Ertrags sowie zur Veränderung des Blühverhaltens bei Pflanzen |
-
1997
- 1997-07-30 WO PCT/US1997/013358 patent/WO1998004725A1/fr not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO1996014414A1 (fr) * | 1994-11-02 | 1996-05-17 | John Innes Centre Innovations Limited | Regulation genetique du fleurissement |
DE4444460A1 (de) * | 1994-11-29 | 1996-05-30 | Inst Genbiologische Forschung | Verfahren zur Steigerung des Ertrags sowie zur Veränderung des Blühverhaltens bei Pflanzen |
Non-Patent Citations (7)
Title |
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FINNEGAN, E.J., ET AL .: "ISOLATION AND IDENTIFICATION BY SEQUENCE HOMOLOGY OF A PUTATIVE CYTOSINE METHYLTRANSFERASE FROM ARABIDOPSIS THALIANA", NUCLEIC ACID RESEARCH, vol. 21, no. 10, 1993, pages 2383 - 2388, XP002049120 * |
FINNEGAN, E.J., ET AL.: "REDUCED DNA METHYLATION IN ARABIDOPSIS THALIANA RESULTS IN ABNORMAL PLANT DEVELOPMENT", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE USA, vol. 93, August 1996 (1996-08-01), pages 8449 - 8454, XP002049122 * |
FINNEGAN, E.J.: "THE ROLE OF DNA METHYLATION IN PLANT DEVELOPMENT", EPIGENETIC MECHANISMS OF GENE REGULATION, vol. 32, 1996, COLD SPRING HARBOUR LABORATORY PRESS, pages 127 - 140, XP002049579 * |
KAKUTANI, T., ET AL .: "CHARACTERIZATION OF AN ARABIDOPSIS THALIANA DNA HYPOMETHYLATION MUTANT", NUCLEIC ACID RESEARCH, vol. 23, no. 1, 1995, pages 130 - 137, XP002049118 * |
RICHARDS, E.J., ET AL.: "DNA METHYLATION AND PLANT DEVELOPMENT", TRENDS IN GENETICS, vol. 13, no. 8, August 1997 (1997-08-01), pages 319 - 323, XP002049123 * |
RONEMUS, M.J., ET AL.: "DEMETHYLATION-INDUCED DEVELOPMENTAL PLEIOTROPY IN ARABIDOPSIS", SCIENCE, vol. 273, 2 August 1996 (1996-08-02), pages 654 - 657, XP002049121 * |
VONGS, A., ET AL.: "ARABIDOPSIS THALIANA DNA METHYLATION MUTANTS", SCIENCE, vol. 260, 25 July 1993 (1993-07-25), pages 1926 - 1928, XP002049119 * |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7138384B1 (en) | 1997-08-29 | 2006-11-21 | The Regents Of The University Of California | Modulators of DNA cytosine-5 methyltransferase and methods for use thereof |
US7125857B2 (en) | 1997-08-29 | 2006-10-24 | The Regents Of The University Of California | Modulators of DNA cytosine-5 methyltransferase and methods for use thereof |
WO1999055891A1 (fr) * | 1998-04-30 | 1999-11-04 | Washington University | Gene vegetal regulant la methylation de l'adn |
EP1082417A4 (fr) * | 1998-06-04 | 2006-08-23 | Univ Queensland | Sequences genetiques modifiant le phenotype |
EP1082417A1 (fr) * | 1998-06-04 | 2001-03-14 | The University Of Queensland | Sequences genetiques modifiant le phenotype |
WO2001000801A2 (fr) * | 1999-06-23 | 2001-01-04 | Syngenta Participations Ag | Gene intervenant dans l'inhibition epigenetique d'un gene |
WO2001000801A3 (fr) * | 1999-06-23 | 2001-05-10 | Syngenta Participations Ag | Gene intervenant dans l'inhibition epigenetique d'un gene |
EP1690943A1 (fr) * | 1999-07-30 | 2006-08-16 | University Of Bath | Plantes mofidiées |
WO2001009299A3 (fr) * | 1999-07-30 | 2001-08-23 | Univ Bath | Plantes modifiees |
WO2001009299A2 (fr) * | 1999-07-30 | 2001-02-08 | University Of Bath | Plantes modifiees |
US7759546B2 (en) | 1999-07-30 | 2010-07-20 | University Of Bath | Methods for modifying plant endosperm |
WO2005038040A3 (fr) * | 2003-10-14 | 2006-11-09 | Ceres Inc | Procedes et compositions permettant de modifier les phenotypes des semences |
US7429692B2 (en) | 2004-10-14 | 2008-09-30 | Ceres, Inc. | Sucrose synthase 3 promoter from rice and uses thereof |
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