WO1996016535A1 - Procede de mise en culture de tissus vegetaux - Google Patents

Procede de mise en culture de tissus vegetaux Download PDF

Info

Publication number
WO1996016535A1
WO1996016535A1 PCT/AU1995/000795 AU9500795W WO9616535A1 WO 1996016535 A1 WO1996016535 A1 WO 1996016535A1 AU 9500795 W AU9500795 W AU 9500795W WO 9616535 A1 WO9616535 A1 WO 9616535A1
Authority
WO
WIPO (PCT)
Prior art keywords
vessel
oxygen
carbon dioxide
scavenger compound
light
Prior art date
Application number
PCT/AU1995/000795
Other languages
English (en)
Inventor
Jennifer Jean Jobling
Michael Laurence Rooney
Original Assignee
Commonwealth Scientific And Industrial Research Organisation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commonwealth Scientific And Industrial Research Organisation filed Critical Commonwealth Scientific And Industrial Research Organisation
Priority to AU39745/95A priority Critical patent/AU3974595A/en
Publication of WO1996016535A1 publication Critical patent/WO1996016535A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/02—Treatment of plants with carbon dioxide

Definitions

  • This invention relates to methods and vessels for the culture of plant tissue.
  • plant tissue culture begins with the elimination of contaminating microorganisms from tissue taken from a parent plant. After culturing and propagation, the plantlets are then reintroduced to microorganisms when they are set in soil for further growth and development. During culture, the tissues are kept in closed, aseptic environments such as in test tubes, agar plates or jars, to which nutrients, hormones and the like are added as required. As conventionally practised, this stage promotes what is known as heterotrophic growth, that is, where the carbon essential for growth is derived from complex compounds in the culture medium. The main source of carbon is usually sucrose or other sugars in the medium.
  • the present invention seeks to avoid at least some of the disadvantages of the prior art by providing a simple, inexpensive and convenient means for supplying carbon dioxide to individual tissue culture vessels.
  • the invention is based on compositions which will generate carbon dioxide in a readily controlled way.
  • the present invention provides a method for the culture of plant propagules comprising: incubating at least one plant propagule within a closed culture vessel containing suitable culture media and a headspace, wherein the vessel incorporates or further contains a composition capable, upon irradiation with light of a selected wavelength, of generating and releasing carbon dioxide into the media and/or headspace.
  • the present invention provides a closeable vessel for culture of plant propagules, which in its closed form defines a space, wherein the vessel incorporates a composition capable, upon irradiation with light of a selected wavelength, of generating and releasing carbon dioxide into said space.
  • the present invention provides a device which may be located into a closeable vessel for culture of plant propagules, incorporating a composition capable, upon irradiation with light of a selected wavelength, of generating and releasing carbon dioxide.
  • the carbon dioxide-releasing composition comprises an oxygen-scavenger compound(s) together with a photosensitiser(s) , which interact when irradiated with light of a selected wavelength (conveniently visible, ultraviolet or near inferred) to consume oxygen and produce carbon dioxide.
  • oxygen scavenger we mean a substance which removes molecular oxygen (by chemical reaction) from the gaseous or dissolved state.
  • the oxygen-scavenger (which provides the carbon dioxide) compound(s) is one which decarboxy1ates when subjected to photosensitized oxidation, typically it will be a heterocyclic carboxylic acid or salt thereof; particularly preferred compounds include furan-2- carboxylic acid, its salts, and derivatives of that acid or its salts, especially those substituted at the 3, 4 or 5 position on the furan ring (e.g. 5-hexadecyloxy-2-furoic acid) , and including polymers in which the furan ring or its derivative is polymer bound.
  • Such non-limiting examples of polymers onto which the furan-2-carboxylic acid is bound include polyhydroxy polymers such as polyvinyl alcohol and its copolymers with other vinyl monomers, and polymers containing carbonyl groups such as dialdehyde starch and polymers of vinyl ketone and the copolymers of that monomer with other monomers.
  • the furan-2-carboxylic acid is bound through chemical bonds in the 3, 4 or 5 position on the ring. Binding includes for instance ether or acetal bonds, among others.
  • Suitable providers of photosensitised oxygen are dyes or compounds known as single oxygen sensitisers, and generally will be those with triplet energies greater than 22 kcal/mole.
  • photosensitisers dyes or compounds known as single oxygen sensitisers, and generally will be those with triplet energies greater than 22 kcal/mole.
  • such compounds should (i) result in intersystem crossing yields able to sensitise sufficient oxygen to the single excited state to bring about oxidation of the carbon dioxide source, and (ii) have a triplet lifetime which will allow enough oxygen to reach it to sustain a useful oxidation rate.
  • Photosensitisers having such characteristics may be found in the classes of porphyrins, thiazines, xanthenes, safranines, acridines, flavins, triarylmethanes, quinones, condensed aromatic compounds and some ketone ⁇ , although other dye compounds offering a suitably high triplet yield and/or long triplet lifetime should also be effective.
  • the radiation must be of an appropriate wavelength for absorption by the photosensitiser(s) , or must be able to be transferred to the photosensitiser from another dye(s). The latter instance would involve the use of a dye(s), the primary role of which would be to harvest energy for transfer to another dye(s) which acted as the sensitiser(s) .
  • the radiation must be of an intensity such as to produce the appropriate rate of formation of single oxygen when absorbed by the sensitiser(s) .
  • Suitable sources include natural lighting, fluorescent lamps, discharge lamps or incandescent lamps, the major requirement being the emission of radiation which includes wavelengths absorbable by the photosensitiser in the preferable UV, visible or near IR spectrum.
  • compositions such as discussed above are in the main readily incorporated into polymer and other substrates, they are therefore adapted to be components of the material from which to make structural features, such as walls and closures, of tissue culture vessels from where carbon dioxide can be released into the media and/or headspace of the vessel.
  • These structural features may consist of a single layer or may be multi-layered structures formed by lamination or co-extrusion or by printing.
  • the active ingredients may be in one or more of these layers including the adhesive coating or printing layer, if present.
  • the compositions might be applied as a lining to a vessel, or be provided in a device such as a capsule or impregnated strip inserted or attached to the vessel where and when convenient.
  • photosensitised oxygen may be toxic to plant propagules
  • the composition is preferably used in a form whereby direct contact between generated photosensitised oxygen and plant propagules in the culture vessel, is avoided.
  • the composition Upon irradiation the composition preferably provides a carbon dioxide concentration within the vessel in the range of about 0.3-2.0%.
  • the culture vessel since levels above about 2% can be counterproductive to the aims of enhancing growth and hardening in cultured plantlets, it may be desirable for the culture vessel to embody means for reducing the concentration of carbon dioxide in the headspace. While it may be possible to arrange for the uptake of carbon dioxide by the plantlets during the period of irradiation to balance gas generation, to avoid an undesirable build up it is preferable that the vessel is provided with means to ensure that some or all of the residual carbon dioxide is eliminated during subsequent periods when uptake by the plantlets ceases. Accordingly, the vessel may be vented or at least be partially permeable to carbon dioxide. Alternatively, chemical reactants or adsorbents for carbon dioxide, such as metallic oxides or hydroxides, could be employed.
  • the culture vessels of this invention should, of course, be at least partially transparent to the activating radiation for the chosen sensitisers and/or other dyes.
  • the present invention is singularly compatible with the culture of plantlets from tissue, in that successful culture requires sequential periods of illumination and darkness.
  • plants grown at the levels of light intensity typical of tissue culture rooms require carbon dioxide roughly in proportion to light intensity.
  • the containers of this invention will automatically provide carbon dioxide as soon as they are illuminated.
  • a feature of the compositions described herein is that they may be configured to provide carbon dioxide in proportion to light intensity.
  • Figure 1 provides the results of an experiment conducted using a piece of film which liberates carbon dioxide upon irradiation with fluorescent light.
  • the film was sealed within an impermeable dish, the top surface of which was sealed with carbon dioxide-permeable oriented polypropylene.
  • the results demonstrate that carbon dioxide levels within the desired range can be achieved.
  • Figure 2 provides the results of experiments to determine the effect of surface area on the rate of carbon dioxide production. It was found that the rate of production of carbon dioxide was found to be affected by the surface area of the film tested. The rate of production of carbon dioxide was compared between one film which was made up of three layers and another film which was three layers with the edge sealed. Both films were exposed to the same area of incident light and the films were exposed to incandescent light from a slide projector
  • Figure 3 provides the result of experiments carried out to determine the effect of light intensity and wavelength on the rate of carbon dioxide production. It was found that the rate of production of carbon dioxide was greater when the film was exposed to fluorescent light compared to incandescent light. The films which were illuminated with fluorescent light at a lower intensity
  • the film with four layers has a greater rate of production than did a single layer of film.
  • a level of about 9% carbon dioxide was maintained in the package as the film 8
  • Figure 4 provides the results of experiments carried out to assess the effect of light intensity on carbon dioxide production. The results showed that the rate of production of carbon dioxide was also affected by light intensity. A single layer of film exposed to fluorescent
  • Figure 5 provides a demonstration of the effect of illumination and darkness on carbon dioxide levels. It was found that the level of carbon dioxide gradually increased when the film was illuminated with fluorescent light (3.4 x 10 -4 watts cm-2) when the lights were turned off the level of carbon dioxide dropped. The rate at which the carbon dioxide level increases and decreases is dependent on the rate of permeation through the barrier film.
  • the bags used in this example were made of oriented polypropylene with a polypropylene micro porous patch.
  • a film was made using ethyl cellulose(lg) , ethyl
  • a piece of the film was sealed inside an impermeable dish the top surface of which was sealed with carbon dioxide-permeable oriented polypropylene.
  • the dish was continuously irradiated with fluorescent light, and samples of the contained gas were taken with a syringe for analysis by gas chromatography.
  • Figure 1 From which it will be seen that a steady carbon dioxide level of approximately 0.1% was attained within less than 3 days and maintained for several days. The rate of production and the rate of leakage reached a steady state where the level in the dish was held within the required concentration range.
  • a film was made using ethyl cellulose(lg) , ethyl
  • Impermeable bags were made up containing (i) three layers of unsealed film, and (ii) three layers of film with the edges sealed.
  • the bags were exposed to the same area of incident light from an incandescent slide projector lamp, and samples of gas taken for analysis to allow comparison of the rates of carbon dioxide accumulation.
  • the results ( Figure 2) showed that the unsealed film gave the greater rate of carbon dioxide production, which points to a relationship between carbon dioxide production and surface area of film.
  • Example 3 Pieces of the film described in Example 2 were placed inside packages which were illuminated with fluorescent light at intensity 3.0 x 10 -3 watts cm-2 or with incandescent light at intensity 11 x 10 -3 watts, cm
  • Example 5 A film was made was made using ethyl cellulose(lg) , ethyl acetate(4.5g), meso-tetraphenylporphyrin(0.5mL 10 -4
  • Bags made up from oriented polypropylene (50 microns) with a polypropylene micro porous patch were illuminated with fluorescent light at intensity 3.4 x 10 -4

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Forests & Forestry (AREA)
  • Ecology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Cette invention concerne un procédé de mise en culture de propagules végéaux qui consiste à placer au moins un propagule végétal en incubation dans un récipient de culture clos contenant un milieu de culture approprié. Ce récipient dispose d'un espace supérieur dans lequel on peut introduire, ou contenant déjà, une composition qui est capable, lorsqu'elle est exposé à une lumière d'une longueur d'onde donnée, de générer et libérer du dioxyde de carbone dans le milieu de culture, dans l'espace supérieur, voire dans les deux. Cette invention concerne également des récipients pouvant être fermés, ainsi que des dispositifs qui renferment la compositon.
PCT/AU1995/000795 1994-11-29 1995-11-29 Procede de mise en culture de tissus vegetaux WO1996016535A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU39745/95A AU3974595A (en) 1994-11-29 1995-11-29 Method of plant tissue culture

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPM9734A AUPM973494A0 (en) 1994-11-29 1994-11-29 Method of plant tissue culture
AUPM9734 1994-11-29

Publications (1)

Publication Number Publication Date
WO1996016535A1 true WO1996016535A1 (fr) 1996-06-06

Family

ID=3784244

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU1995/000795 WO1996016535A1 (fr) 1994-11-29 1995-11-29 Procede de mise en culture de tissus vegetaux

Country Status (2)

Country Link
AU (1) AUPM973494A0 (fr)
WO (1) WO1996016535A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014012968A1 (fr) * 2012-07-17 2014-01-23 Antecy B.V. Procédé pour accélérer la croissance de plantes dans un environnement contrôlé

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5491439A (en) * 1977-12-22 1979-07-19 Matsushita Electric Ind Co Ltd Carbon dioxide fertilizing device
DE3215958A1 (de) * 1982-04-29 1983-11-03 Elisabeth 3000 Hannover Biener Verfahren zur kohlensaeureduengung von wasserpflanzen
JPS62210926A (ja) * 1986-03-11 1987-09-17 エクソン　ケミカル　パテンツ　インコ−ポレ−テツド 植物の収穫を向上する方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5491439A (en) * 1977-12-22 1979-07-19 Matsushita Electric Ind Co Ltd Carbon dioxide fertilizing device
DE3215958A1 (de) * 1982-04-29 1983-11-03 Elisabeth 3000 Hannover Biener Verfahren zur kohlensaeureduengung von wasserpflanzen
JPS62210926A (ja) * 1986-03-11 1987-09-17 エクソン　ケミカル　パテンツ　インコ−ポレ−テツド 植物の収穫を向上する方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DERWENT WPAT ONLINE ABSTRACT, Accession No. 79-63869B; & JP,A,54 091 439, (MATSUSHITA ELEC IND KK), 19 July 1979. *
DERWENT WPAT ONLINE ABSTRACT, Accession No. 87-302136; & JP,A,62 210 926, (EXXON CHEM PAT INC), 17 September 1987. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014012968A1 (fr) * 2012-07-17 2014-01-23 Antecy B.V. Procédé pour accélérer la croissance de plantes dans un environnement contrôlé

Also Published As

Publication number Publication date
AUPM973494A0 (en) 1994-12-22

Similar Documents

Publication Publication Date Title
ES2220910T3 (es) Eliminadores de oxieno independientes de catalizadores de metales.
Burchard et al. Light-induced lysis and carotenogenesis in Myxococcus xanthus
US6821482B1 (en) Triggered active packaging material
CA2071079A1 (fr) Methodes et compositions pour la desoxygenation
CN102026719A (zh) 从外源进行内部能量活化的等离子体激元辅助系统和方法
JP2006502926A (ja) 脱酸素剤を賦活化し、保存し、かつ配送するための方法、および保存された脱酸素剤
JP7462139B2 (ja) 植物処理装置
WO2019031559A1 (fr) Procédé de culture de plante et dispositif de culture de plante
JPS6418441A (en) Preparation of organic amorphous film
EA026710B1 (ru) Предотвращение образования 3-метилбут-2-ен-1-тиол (мбт) в напитке на основе хмеля
WO1996016535A1 (fr) Procede de mise en culture de tissus vegetaux
Miles et al. A rapid screening technique for photosynthetic mutants of higher plants
CN101253882B (zh) 基于单线态氧氧化作用的果蔬保鲜技术
EP0246013A3 (fr) Procédé de fabrication d'un film de collagène
EA023352B1 (ru) Устранение образования 3-метилбут-2-ен-1-тиола (мбт) в напитке на основе хмеля
JP5936372B2 (ja) 植物育成方法および植物育成材料
CN1004281B (zh) 酒的红外线催陈方法及其设备
RU3547U1 (ru) Устройство для стерилизации крови и биологических жидкостей
AU711046B2 (en) Triggered active packaging material
JP2006316037A (ja) 抗菌防腐シートおよび抗菌防腐方法
JP2003210555A (ja) プラスチック容器の殺菌方法
AU672661B2 (en) Oxygen scavengers independent of transition metal catalysts
AU672661C (en) Oxygen scavengers independent of transition metal catalysts
JPH0513197U (ja) 光合成生物の培養装置
Taysum A simple apparatus for the collection of Hevea latex under aseptic conditions

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AL AM AT AU BB BG BR BY CA CH CN CZ DE DK EE ES FI GB GE HU IS JP KE KG KP KR KZ LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK TJ TM TT UA UG US UZ VN

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): KE LS MW SD SZ UG AT BE CH DE DK ES FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 08836491

Country of ref document: US

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA