WO1996003882A9 - - Google Patents

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Publication number
WO1996003882A9
WO1996003882A9 WO9603882A9 WO 1996003882 A9 WO1996003882 A9 WO 1996003882A9 WO 9603882 A9 WO9603882 A9 WO 9603882A9
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WO
WIPO (PCT)
Prior art keywords
oil
herbicide
microorganism
silicone
present
Prior art date
Application number
Other languages
English (en)
Japanese (ja)
Publication date

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Definitions

  • the present invention relates to a herbicide comprising a microorganism having weed control ability and at least one of vegetable oil, mineral oil and silicone as an active ingredient, and a herbicidal method using the herbicide.
  • Aeschynomene is commercially available for controlling legume weeds in paddy fields and soybean fields.
  • Lu-boa No. 2 which used Colletotoric mgleosporioides for controlling P. vulgaris parasitizing soybean in China, and in Canada for controlling Marbazenioi which is a problem weed such as wheat Colletotrichum gloeosporioides f. Sp. BioMal (Pilom Bios) having lva as an active ingredient is commercially available.
  • spores that can be dried can be easily formulated as a powdery wettable powder and the like, but in this case, it is necessary to dilute with water etc. before use, so considerable labor is required.
  • Collego is sold as a powder containing spore, which is an active ingredient, and a solution containing sucrose for suspending it, and these two components are mixed and diluted with water at the time of use. There is a need to. Also, these dosage forms cause dusting.
  • DeVine which is currently commercialized in the form of cell concentrate, has no problem like Collego, but has a problem that long-term storage is impossible (Weed Science) 34 CS Uppl. 1) (1986): 15-16).
  • the object of the present invention is to solve the problems of such conventional herbicides and methods, specifically, there is no need to dilute in water at the time of use, and the storage stability is excellent. To provide a microbial herbicide.
  • composition comprising oils such as vegetable oil, mineral oil and silicone is effective for controlling weeds in paddy fields, and filed a petition earlier. Hei 5- 333087).
  • the inventors of the present invention have found that the microorganisms can survive for a long period of time in these oils, and the oils have the ability to control weeds possessed by the microorganisms. It has been found that the present invention improves the present invention.
  • the first of the present invention is a herbicide comprising a microorganism having weed control ability, and at least one component of vegetable oil, mineral oil and silicone as active ingredients.
  • the microorganism having weed control ability which can be used here include microorganisms belonging to the genus Exocellus or Calvularia, and among these, Exemple hyrum • monoceras can be exemplified as a suitable microorganism.
  • weeds of the genus can be mentioned as weeds targeted.
  • a second aspect of the present invention is a weeding method characterized by applying the above-mentioned herbicide to a paddy field.
  • a specific application method there is a method such as direct drop application to the paddy field.
  • the present invention will be described in detail.
  • the present invention has a composition different from the commercially available microbial herbicide products, and by formulating it according to the present invention, the herbicidal effect of the weed controlling microorganism itself is dramatically improved.
  • the aforementioned microorganism rapidly spreads the water surface of the paddy field and exerts the herbicidal effect uniformly over a wide area.
  • formulation according to the present invention enables long-term preservation of various microorganisms including microorganisms having weed control ability.
  • the present invention provides a microorganism-containing herbicide which is characterized by containing, as an active ingredient, at least one of three components of vegetable oil, mineral oil and silicone, and a microorganism having weed control ability. .
  • the microorganism-containing herbicide of the present invention spreads the water surface rapidly and extremely widely due to the characteristics of these oils by drip spraying directly to one or several power stations on the flooded paddy water surface. Also, they exhibit a herbicidal effect far superior to that of a suspension of cells by a simple aqueous solution of surfactant. This is considered to cause damage to weeds targeted by vegetable oil, mineral oil or silicone itself and to promote the effect of microorganisms having weed control ability.
  • Mineral oils which can be used in the present invention include spindle oil, heavy white oil, light white oil, mineral spirits, mineral turpentine, naphthenic oil, paraffin oil, agricultural machine oil etc. It is not something that
  • silicone oils examples include soybean oil, rapeseed oil, castor oil, cottonseed oil, palm oil, safflower oil, and the like.
  • silicone oil or silicone oil is generally used. Specifically, silicone oil TSF-45 ⁇ 10 (Toshiba Silicone Co., Ltd.), silicone oil SAG-47 One company), etc. may be mentioned, but it is not limited thereto.
  • the microorganism used in the present invention is not particularly limited as long as it is a microorganism having weed control ability, but it is preferable to use a microorganism belonging to the genus Oxalus or Calbraria, and in particular, a microorganism belonging to Oxalus monophyllus monoceras is used Is preferred.
  • WEXE mouth Hiram 'monoceras B026 (Exsero ilum monoceras B026) (FERM BP-4215)
  • WEXE mouth Hiram Monoceras B232 (Exserohilum monoceras B232) (FERM BP-4217)
  • Hexese port HIRAM ⁇ MONOCERAS B263 (Exserohi lum monoceras B263)
  • FERM BP-4218 executorium lam monoceras B267 (Exserohi lum monoceras B267) (FERM BP-4219), quesero high la '' ⁇ ⁇ ⁇ 276 B276 (Exserohi lum monoceras (FERM BP-4220), Calvaria spp.
  • the formulation according to the present invention improves such physical properties and facilitates handling.
  • the content is 1 ⁇ 10 3 to 1 ⁇ 10 1 1 value Z liters, preferably 1 ⁇ 10 5 to 1 10 ′ ° It is a single item.
  • the content thereof is 1 X 10 5 ⁇ 1 X 10 '5 pieces glue Tsu Torr, preferably 1 chi 10' in ° ⁇ 1 X 10 1 2 pieces glue Tsu Torr is there.
  • Nitrogen sources useful for growth nitrate, ammonia, amino acids etc.
  • carbon sources, or substances that promote spore germination carboxymethylcellulose sodium salt, sodium naphthalene sulfonate formalin condensate, polyethylene glycol etc
  • moisturizers polysaccharides such as hyaluronic acid, collagen, proteins such as lecithin, glycerin, fats and oils, etc.
  • the composition ratio of both components is 99.5% by weight or less, preferably 50.about.99.5% by weight of mineral oil, and 60% by weight or less of vegetable oil. Preferably, it is 0.5 to 50.0% by weight.
  • the composition ratio of the two components is 99.5% by weight or less of mineral oil, preferably 85 to 99.5% by weight, and 15.0% by weight of silicone. % Or less, preferably 0.5 to 15.0% by weight.
  • the composition ratio of both components is 99.5% by weight or less of vegetable oil, preferably 85 to 99.5% by weight, and 15.0% by weight silicone. Below, Preferably it is 0 5-15.0 weight%.
  • the composition ratio is 99.5% by weight or less of mineral oil, preferably 50 to 99.5% by weight.
  • the vegetable oil is 60.0% by weight or less, preferably 0.025 to 45.0% by weight
  • the silicone is 10.0% by weight or less, preferably 0.25 to 5.0% by weight.
  • the herbicidal composition of the present invention is effective against all weeds occurring in the paddy field, and is effective against grass weeds, especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
  • grass weeds especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
  • grass weeds especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
  • grass weeds especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
  • the growth stage of the plant to be weeded, but it is particularly effective for the early stage of growth when the whole plant is submerged in paddy water.
  • the application method is not particularly limited as long as it is a method capable of effectively controlling weeds, but it is desirable to spray directly on the paddy field under flooded water.
  • the timing of use of the herbicide of the present invention is not particularly limited. It is desirable to use it after puddling, preferably in the early stage after rice planting. Also, when this herbicide is applied in the field, the amount of application is the amount of conidia of Ex. 1 x 10 2 to 1 10 ' 5 pieces of 10 thal, preferably 1 x 10 5 to 1 x 10 11 pieces / 0 are, and the amount of conidia of Calbraria bacteria is 1 x 10 4 to 1 x 10 ' 9 pieces' 10 ares, preferably 1 ⁇ 10 ⁇ 1 1 x 10 13 per 10 ares.
  • Monoceras 026 is grown on potato dextrose agar medium, and the formed conidia are collected and dried.
  • a suspension with a spore concentration of 1 ⁇ 10 7 / ml was prepared by mixing into the composition.
  • Triton X-100 aqueous solution Triton X-100 aqueous solution
  • a spore suspension (spore concentration 1 xiO T / ml) according to (0.02% by weight) was simultaneously prepared.
  • Triton X-100 aqueous solution 4 stored 0 0 0 spore suspension 2 5 stored 0 0 0
  • the herbicide of the present invention was found to be very suitable for the long-term preservation of microorganisms.
  • the higher the storage temperature the better the preservation of vesicles compared to other storage methods.
  • Treatment B Recovered and dried in the same manner as in Example 1 Hyox Monoceras B026
  • Treatment C spindle oil, castor oil, silicone oil (Toshiba Silicone Co., Ltd.)
  • Process D Conidia 4 X10 4 amino Ekise mouth Hiram monoceras B026 dried recovered in the same manner as in Example 1 (4 X10 8 cells Z are), spindle oil, shed we oil, silicone oil (Toshiba Silicone Co. ) Suspended in oil composition 10/1 (lOOmlZ are) formulated at a weight ratio of 95: 4 ⁇ ⁇ 1 and dropped, The treatments A to D were applied to pots grown ten by ten each. The growth condition of Nobi was compared and observed between the treated pot and the untreated pot for 3 weeks after the dripping treatment. At that time, the degree of growth inhibition was determined at a percentage where 0% was the same as that of nobi and no growth was observed in untreated pods, and 100% when the growth was completely inhibited. The results are shown in Table 2.
  • the day of the treatment date was day 0.
  • Treatment F Cultivated in the same manner as in Examus hyrum monocellus B026, and recovered, dried force Lubraria spp. B-261 conidia 1 X 10 6 (1 X 10 1 "noirs), 0.02% Triton X- It was suspended in 100 aqueous solution 10 / zl (lOOmlZ are) and added dropwise.
  • Treatment G Spindle oil, castor oil, silicone oil (Toshiba Silicone Co., Ltd.) A 10 / zl (lOOml Zal) was added dropwise to a liquid formulated in a weight ratio of 95: 4: 1.
  • Treatment H Recovered and dried Calbraria sp. B-261 conidia 1 ⁇ 10 6 (1 ⁇ 10 1 D pieces) as above, and weigh spindle oil, castor oil, silicone oil (Toshiba Silicone Co., Ltd.) The oil composition was mixed at a ratio of 95: 4: 1 and suspended with a composition 10 // 1 (lOOmlZ are) and added dropwise.
  • the 0th day was the day of treatment.
  • the conidia 7.5 ⁇ 10 5 (10 8 ares) of the conidia were suspended in 0.02% Triton X-100 aqueous solution (750 1 UOO ml Z are) and added dropwise.
  • Treatment 3 Oil composition containing spindle oil, castor oil and silicone oil (Toshiba Silicone Co.) in a weight ratio of 95: 4: 1 was added dropwise to 75 1 (lOml Zall).
  • the day of drip treatment was designated as day 0.
  • the formulation inhibition according to the present invention dramatically improves the weed growth inhibition effect of plant pathogens.
  • the composition according to the present invention spreads the water surface by dripping onto a force of a paddy field under spring water and exerts the effect uniformly over a wide range. Treatments 1 to 4 had no effect on the growth of rice.

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