WO1996003882A9 - - Google Patents
Info
- Publication number
- WO1996003882A9 WO1996003882A9 WO9603882A9 WO 1996003882 A9 WO1996003882 A9 WO 1996003882A9 WO 9603882 A9 WO9603882 A9 WO 9603882A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- oil
- herbicide
- microorganism
- silicone
- present
- Prior art date
Links
- 241000196324 Embryophyta Species 0.000 claims description 42
- 230000002363 herbicidal Effects 0.000 claims description 38
- 239000004009 herbicide Substances 0.000 claims description 33
- 244000005700 microbiome Species 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 229920001296 polysiloxane Polymers 0.000 claims description 23
- 239000002480 mineral oil Substances 0.000 claims description 15
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 15
- 239000008158 vegetable oil Substances 0.000 claims description 15
- 235000010446 mineral oil Nutrition 0.000 claims description 14
- 239000004480 active ingredient Substances 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 3
- 238000009333 weeding Methods 0.000 claims description 3
- LIKMAJRDDDTEIG-UHFFFAOYSA-N 1-Hexene Chemical compound CCCCC=C LIKMAJRDDDTEIG-UHFFFAOYSA-N 0.000 claims 1
- YZHUMGUJCQRKBT-UHFFFAOYSA-M Sodium chlorate Chemical compound [Na+].[O-]Cl(=O)=O YZHUMGUJCQRKBT-UHFFFAOYSA-M 0.000 claims 1
- 230000012010 growth Effects 0.000 description 37
- 239000003921 oil Substances 0.000 description 37
- 235000019198 oils Nutrition 0.000 description 37
- 239000000203 mixture Substances 0.000 description 33
- 230000002401 inhibitory effect Effects 0.000 description 25
- 238000003860 storage Methods 0.000 description 17
- 230000000694 effects Effects 0.000 description 15
- 210000004215 spores Anatomy 0.000 description 13
- 210000004027 cells Anatomy 0.000 description 12
- 229920002545 silicone oil Polymers 0.000 description 11
- 238000002360 preparation method Methods 0.000 description 9
- 229920004890 Triton X-100 Polymers 0.000 description 8
- 239000007864 aqueous solution Substances 0.000 description 8
- 239000000725 suspension Substances 0.000 description 7
- 239000004359 castor oil Substances 0.000 description 6
- 235000019438 castor oil Nutrition 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 6
- 240000007594 Oryza sativa Species 0.000 description 5
- 235000007164 Oryza sativa Nutrition 0.000 description 5
- 244000052616 bacterial pathogens Species 0.000 description 5
- 238000009472 formulation Methods 0.000 description 5
- 235000009566 rice Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 241000371679 Setosphaeria monoceras Species 0.000 description 4
- 230000001580 bacterial Effects 0.000 description 4
- VLKZOEOYAKHREP-UHFFFAOYSA-N hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000001717 pathogenic Effects 0.000 description 4
- 244000052769 pathogens Species 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N D-sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- CZMRCDWAGMRECN-GDQSFJPYSA-N Sucrose Natural products O([C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O1)[C@@]1(CO)[C@H](O)[C@@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-GDQSFJPYSA-N 0.000 description 3
- 239000002609 media Substances 0.000 description 3
- 230000000813 microbial Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 230000004763 spore germination Effects 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 101710006658 AAEL003512 Proteins 0.000 description 2
- 241001529387 Colletotrichum gloeosporioides Species 0.000 description 2
- 240000002436 Echinochloa crus-galli Species 0.000 description 2
- 240000007842 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000008079 hexane Substances 0.000 description 2
- 230000002209 hydrophobic Effects 0.000 description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 239000001965 potato dextrose agar Substances 0.000 description 2
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 1
- 241000711295 Aeria Species 0.000 description 1
- 241000212906 Aeschynomene Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 240000000218 Cannabis sativa Species 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 241000223211 Curvularia lunata Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 210000003298 Dental Enamel Anatomy 0.000 description 1
- 240000005638 Galinsoga parviflora Species 0.000 description 1
- 241001573459 Hymenia Species 0.000 description 1
- 229940067606 Lecithin Drugs 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- 241001342584 Monophyllus Species 0.000 description 1
- 206010054107 Nodule Diseases 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 239000005662 Paraffin oil Substances 0.000 description 1
- 240000005158 Phaseolus vulgaris Species 0.000 description 1
- 241000233637 Phytophthora palmivora Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 235000019484 Rapeseed oil Nutrition 0.000 description 1
- 235000019485 Safflower oil Nutrition 0.000 description 1
- 241000779819 Syncarpia glomulifera Species 0.000 description 1
- FPKOPBFLPLFWAD-UHFFFAOYSA-N Trinitrotoluene Chemical compound CC1=CC=C([N+]([O-])=O)C([N+]([O-])=O)=C1[N+]([O-])=O FPKOPBFLPLFWAD-UHFFFAOYSA-N 0.000 description 1
- 240000008529 Triticum aestivum Species 0.000 description 1
- 229940036248 Turpentine Drugs 0.000 description 1
- 241001148683 Zostera marina Species 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical class [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 230000003042 antagnostic Effects 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 229960005188 collagen Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000000875 corresponding Effects 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000010410 dusting Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Polymers 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000010721 machine oil Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 230000001338 necrotic Effects 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000001739 pinus spp. Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000002250 progressing Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 235000005713 safflower oil Nutrition 0.000 description 1
- 239000003813 safflower oil Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- MAKUBRYLFHZREJ-JWBQXVCJSA-M sodium;(2S,3S,4R,5R,6R)-3-[(2S,3R,5S,6R)-3-acetamido-5-hydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5,6-trihydroxyoxane-2-carboxylate Chemical compound [Na+].CC(=O)N[C@@H]1C[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](C([O-])=O)O[C@@H](O)[C@H](O)[C@H]1O MAKUBRYLFHZREJ-JWBQXVCJSA-M 0.000 description 1
- HIEHAIZHJZLEPQ-UHFFFAOYSA-M sodium;naphthalene-1-sulfonate Chemical compound [Na+].C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 HIEHAIZHJZLEPQ-UHFFFAOYSA-M 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000004083 survival Effects 0.000 description 1
- 230000002195 synergetic Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
- 235000021307 wheat Nutrition 0.000 description 1
Definitions
- the present invention relates to a herbicide comprising a microorganism having weed control ability and at least one of vegetable oil, mineral oil and silicone as an active ingredient, and a herbicidal method using the herbicide.
- Aeschynomene is commercially available for controlling legume weeds in paddy fields and soybean fields.
- Lu-boa No. 2 which used Colletotoric mgleosporioides for controlling P. vulgaris parasitizing soybean in China, and in Canada for controlling Marbazenioi which is a problem weed such as wheat Colletotrichum gloeosporioides f. Sp. BioMal (Pilom Bios) having lva as an active ingredient is commercially available.
- spores that can be dried can be easily formulated as a powdery wettable powder and the like, but in this case, it is necessary to dilute with water etc. before use, so considerable labor is required.
- Collego is sold as a powder containing spore, which is an active ingredient, and a solution containing sucrose for suspending it, and these two components are mixed and diluted with water at the time of use. There is a need to. Also, these dosage forms cause dusting.
- DeVine which is currently commercialized in the form of cell concentrate, has no problem like Collego, but has a problem that long-term storage is impossible (Weed Science) 34 CS Uppl. 1) (1986): 15-16).
- the object of the present invention is to solve the problems of such conventional herbicides and methods, specifically, there is no need to dilute in water at the time of use, and the storage stability is excellent. To provide a microbial herbicide.
- composition comprising oils such as vegetable oil, mineral oil and silicone is effective for controlling weeds in paddy fields, and filed a petition earlier. Hei 5- 333087).
- the inventors of the present invention have found that the microorganisms can survive for a long period of time in these oils, and the oils have the ability to control weeds possessed by the microorganisms. It has been found that the present invention improves the present invention.
- the first of the present invention is a herbicide comprising a microorganism having weed control ability, and at least one component of vegetable oil, mineral oil and silicone as active ingredients.
- the microorganism having weed control ability which can be used here include microorganisms belonging to the genus Exocellus or Calvularia, and among these, Exemple hyrum • monoceras can be exemplified as a suitable microorganism.
- weeds of the genus can be mentioned as weeds targeted.
- a second aspect of the present invention is a weeding method characterized by applying the above-mentioned herbicide to a paddy field.
- a specific application method there is a method such as direct drop application to the paddy field.
- the present invention will be described in detail.
- the present invention has a composition different from the commercially available microbial herbicide products, and by formulating it according to the present invention, the herbicidal effect of the weed controlling microorganism itself is dramatically improved.
- the aforementioned microorganism rapidly spreads the water surface of the paddy field and exerts the herbicidal effect uniformly over a wide area.
- formulation according to the present invention enables long-term preservation of various microorganisms including microorganisms having weed control ability.
- the present invention provides a microorganism-containing herbicide which is characterized by containing, as an active ingredient, at least one of three components of vegetable oil, mineral oil and silicone, and a microorganism having weed control ability. .
- the microorganism-containing herbicide of the present invention spreads the water surface rapidly and extremely widely due to the characteristics of these oils by drip spraying directly to one or several power stations on the flooded paddy water surface. Also, they exhibit a herbicidal effect far superior to that of a suspension of cells by a simple aqueous solution of surfactant. This is considered to cause damage to weeds targeted by vegetable oil, mineral oil or silicone itself and to promote the effect of microorganisms having weed control ability.
- Mineral oils which can be used in the present invention include spindle oil, heavy white oil, light white oil, mineral spirits, mineral turpentine, naphthenic oil, paraffin oil, agricultural machine oil etc. It is not something that
- silicone oils examples include soybean oil, rapeseed oil, castor oil, cottonseed oil, palm oil, safflower oil, and the like.
- silicone oil or silicone oil is generally used. Specifically, silicone oil TSF-45 ⁇ 10 (Toshiba Silicone Co., Ltd.), silicone oil SAG-47 One company), etc. may be mentioned, but it is not limited thereto.
- the microorganism used in the present invention is not particularly limited as long as it is a microorganism having weed control ability, but it is preferable to use a microorganism belonging to the genus Oxalus or Calbraria, and in particular, a microorganism belonging to Oxalus monophyllus monoceras is used Is preferred.
- WEXE mouth Hiram 'monoceras B026 (Exsero ilum monoceras B026) (FERM BP-4215)
- WEXE mouth Hiram Monoceras B232 (Exserohilum monoceras B232) (FERM BP-4217)
- Hexese port HIRAM ⁇ MONOCERAS B263 (Exserohi lum monoceras B263)
- FERM BP-4218 executorium lam monoceras B267 (Exserohi lum monoceras B267) (FERM BP-4219), quesero high la '' ⁇ ⁇ ⁇ 276 B276 (Exserohi lum monoceras (FERM BP-4220), Calvaria spp.
- the formulation according to the present invention improves such physical properties and facilitates handling.
- the content is 1 ⁇ 10 3 to 1 ⁇ 10 1 1 value Z liters, preferably 1 ⁇ 10 5 to 1 10 ′ ° It is a single item.
- the content thereof is 1 X 10 5 ⁇ 1 X 10 '5 pieces glue Tsu Torr, preferably 1 chi 10' in ° ⁇ 1 X 10 1 2 pieces glue Tsu Torr is there.
- Nitrogen sources useful for growth nitrate, ammonia, amino acids etc.
- carbon sources, or substances that promote spore germination carboxymethylcellulose sodium salt, sodium naphthalene sulfonate formalin condensate, polyethylene glycol etc
- moisturizers polysaccharides such as hyaluronic acid, collagen, proteins such as lecithin, glycerin, fats and oils, etc.
- the composition ratio of both components is 99.5% by weight or less, preferably 50.about.99.5% by weight of mineral oil, and 60% by weight or less of vegetable oil. Preferably, it is 0.5 to 50.0% by weight.
- the composition ratio of the two components is 99.5% by weight or less of mineral oil, preferably 85 to 99.5% by weight, and 15.0% by weight of silicone. % Or less, preferably 0.5 to 15.0% by weight.
- the composition ratio of both components is 99.5% by weight or less of vegetable oil, preferably 85 to 99.5% by weight, and 15.0% by weight silicone. Below, Preferably it is 0 5-15.0 weight%.
- the composition ratio is 99.5% by weight or less of mineral oil, preferably 50 to 99.5% by weight.
- the vegetable oil is 60.0% by weight or less, preferably 0.025 to 45.0% by weight
- the silicone is 10.0% by weight or less, preferably 0.25 to 5.0% by weight.
- the herbicidal composition of the present invention is effective against all weeds occurring in the paddy field, and is effective against grass weeds, especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
- grass weeds especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
- grass weeds especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
- grass weeds especially weeds such as tree barnyard grasses, Hymenia barnacleus, Hynetra barnulae, and barnyard grasses. is there.
- the growth stage of the plant to be weeded, but it is particularly effective for the early stage of growth when the whole plant is submerged in paddy water.
- the application method is not particularly limited as long as it is a method capable of effectively controlling weeds, but it is desirable to spray directly on the paddy field under flooded water.
- the timing of use of the herbicide of the present invention is not particularly limited. It is desirable to use it after puddling, preferably in the early stage after rice planting. Also, when this herbicide is applied in the field, the amount of application is the amount of conidia of Ex. 1 x 10 2 to 1 10 ' 5 pieces of 10 thal, preferably 1 x 10 5 to 1 x 10 11 pieces / 0 are, and the amount of conidia of Calbraria bacteria is 1 x 10 4 to 1 x 10 ' 9 pieces' 10 ares, preferably 1 ⁇ 10 ⁇ 1 1 x 10 13 per 10 ares.
- Monoceras 026 is grown on potato dextrose agar medium, and the formed conidia are collected and dried.
- a suspension with a spore concentration of 1 ⁇ 10 7 / ml was prepared by mixing into the composition.
- Triton X-100 aqueous solution Triton X-100 aqueous solution
- a spore suspension (spore concentration 1 xiO T / ml) according to (0.02% by weight) was simultaneously prepared.
- Triton X-100 aqueous solution 4 stored 0 0 0 spore suspension 2 5 stored 0 0 0
- the herbicide of the present invention was found to be very suitable for the long-term preservation of microorganisms.
- the higher the storage temperature the better the preservation of vesicles compared to other storage methods.
- Treatment B Recovered and dried in the same manner as in Example 1 Hyox Monoceras B026
- Treatment C spindle oil, castor oil, silicone oil (Toshiba Silicone Co., Ltd.)
- Process D Conidia 4 X10 4 amino Ekise mouth Hiram monoceras B026 dried recovered in the same manner as in Example 1 (4 X10 8 cells Z are), spindle oil, shed we oil, silicone oil (Toshiba Silicone Co. ) Suspended in oil composition 10/1 (lOOmlZ are) formulated at a weight ratio of 95: 4 ⁇ ⁇ 1 and dropped, The treatments A to D were applied to pots grown ten by ten each. The growth condition of Nobi was compared and observed between the treated pot and the untreated pot for 3 weeks after the dripping treatment. At that time, the degree of growth inhibition was determined at a percentage where 0% was the same as that of nobi and no growth was observed in untreated pods, and 100% when the growth was completely inhibited. The results are shown in Table 2.
- the day of the treatment date was day 0.
- Treatment F Cultivated in the same manner as in Examus hyrum monocellus B026, and recovered, dried force Lubraria spp. B-261 conidia 1 X 10 6 (1 X 10 1 "noirs), 0.02% Triton X- It was suspended in 100 aqueous solution 10 / zl (lOOmlZ are) and added dropwise.
- Treatment G Spindle oil, castor oil, silicone oil (Toshiba Silicone Co., Ltd.) A 10 / zl (lOOml Zal) was added dropwise to a liquid formulated in a weight ratio of 95: 4: 1.
- Treatment H Recovered and dried Calbraria sp. B-261 conidia 1 ⁇ 10 6 (1 ⁇ 10 1 D pieces) as above, and weigh spindle oil, castor oil, silicone oil (Toshiba Silicone Co., Ltd.) The oil composition was mixed at a ratio of 95: 4: 1 and suspended with a composition 10 // 1 (lOOmlZ are) and added dropwise.
- the 0th day was the day of treatment.
- the conidia 7.5 ⁇ 10 5 (10 8 ares) of the conidia were suspended in 0.02% Triton X-100 aqueous solution (750 1 UOO ml Z are) and added dropwise.
- Treatment 3 Oil composition containing spindle oil, castor oil and silicone oil (Toshiba Silicone Co.) in a weight ratio of 95: 4: 1 was added dropwise to 75 1 (lOml Zall).
- the day of drip treatment was designated as day 0.
- the formulation inhibition according to the present invention dramatically improves the weed growth inhibition effect of plant pathogens.
- the composition according to the present invention spreads the water surface by dripping onto a force of a paddy field under spring water and exerts the effect uniformly over a wide range. Treatments 1 to 4 had no effect on the growth of rice.
Family
ID=
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