WO1994000576A1 - Vitamin d hydroxylase gene - Google Patents
Vitamin d hydroxylase gene Download PDFInfo
- Publication number
- WO1994000576A1 WO1994000576A1 PCT/JP1993/000856 JP9300856W WO9400576A1 WO 1994000576 A1 WO1994000576 A1 WO 1994000576A1 JP 9300856 W JP9300856 W JP 9300856W WO 9400576 A1 WO9400576 A1 WO 9400576A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- plasmid
- hydroxylase
- dna
- gene
- vitamin
- Prior art date
Links
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- 210000000287 oocyte Anatomy 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229940002612 prodrug Drugs 0.000 description 1
- 239000000651 prodrug Substances 0.000 description 1
- 229960002429 proline Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000010339 sodium tetraborate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 210000002325 somatostatin-secreting cell Anatomy 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000006097 ultraviolet radiation absorber Substances 0.000 description 1
- 150000003703 vitamin D2 derivatives Chemical class 0.000 description 1
- 150000003704 vitamin D3 derivatives Chemical class 0.000 description 1
- 150000003706 vitamin D4 derivatives Chemical class 0.000 description 1
- 150000003707 vitamin D5 derivatives Chemical class 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
- C12Y114/13013—Calcidiol 1-monooxygenase (1.14.13.13), i.e. 25-hydroxyvitamin D-1-alpha-hydroxylase
Definitions
- Figure 1 shows a size of about 3.83 that contains a gene that expresses a hydroxylase activity that hydroxylates position 25 of vitamin D and converts it to 25-hydroxyvitamin D. It is a restriction map of a kbp DNA fragment.
- Hind111, SacI, BclI, KpnI, PstI, and BamHI represent each restriction enzyme, and the number represents the distance between each restriction enzyme cutting position and is expressed in kbp. I have.
- Fig. 6 shows a gene containing a hydroxylase-encoding gene derived from the chromosomal DNA of Nocardia phototrophica N-102 strain cloned into plasmid PHK03. Shows the latter half of the base sequence of the 8366 bp DNA fragment.
- a DNA fragment containing the target gene can be obtained together with other DNA fragments.
- the target gene is obtained from the mixture of DNA fragments obtained in this way.
- the DNA fragment is first incorporated into a vector plasmid that has been treated so that it can bind to the end of the DNA fragment containing the gene of interest.
- Transforming a host bacterium by then produced various recombinant plus Mi de of, for example, a T hior r sorting shown to recombinant plus Mi de-containing transformants.
- a recombinant plasmid containing DNA containing the target gene can be selected.
- nucleotide sequence of the gene encoding hydroxylase had the nucleotide sequence as shown in FIGS. 8 and 9.
- Example 2 Screening of a transformant having the ability to hydroxylate the 25th position of vitamin D and convert it to 25-hide xyximin D Cells were isolated from each colony of the transformed strain obtained in Example 1 using a microorganism conversion medium (1.5% glucose,
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP93913573A EP0653490A4 (en) | 1992-06-25 | 1993-06-24 | VITAMIN D HYDROXYLASE GENE |
AU43574/93A AU4357493A (en) | 1992-06-25 | 1993-06-24 | Vitamin d hydroxylase gene |
KR1019940704606A KR950702247A (ko) | 1992-06-25 | 1994-12-16 | 비타민 d류 히드록시라제유전자 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP4/167644 | 1992-06-25 | ||
JP16764492 | 1992-06-25 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1994000576A1 true WO1994000576A1 (en) | 1994-01-06 |
Family
ID=15853592
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP1993/000856 WO1994000576A1 (en) | 1992-06-25 | 1993-06-24 | Vitamin d hydroxylase gene |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0653490A4 (ja) |
KR (1) | KR950702247A (ja) |
AU (1) | AU4357493A (ja) |
CA (1) | CA2138516A1 (ja) |
WO (1) | WO1994000576A1 (ja) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997004082A1 (en) * | 1995-07-19 | 1997-02-06 | Novo Nordisk A/S | Proteolytic enzymes derived from amycolata |
US5948746A (en) * | 1995-07-19 | 1999-09-07 | Novo Nordisk A/S | Proteolytic enzymes |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK0855445T3 (da) * | 1995-08-28 | 2003-06-30 | Mercian Corp | Fremgangsmåde til fremstilling af hydroxylerede cholesteroler ved biologisk omdannelse og dihydroxycholesteroler |
AU2978597A (en) * | 1996-06-27 | 1998-01-14 | Chugai Seiyaku Kabushiki Kaisha | Biological process for preparing 25-hydroxylated steroids |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02469A (ja) * | 1987-07-08 | 1990-01-05 | Taisho Pharmaceut Co Ltd | ビタミンd類の製造方法 |
JPH02231089A (ja) * | 1989-03-04 | 1990-09-13 | Taisho Pharmaceut Co Ltd | ビタミンd類の製造方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5212296A (en) * | 1989-09-11 | 1993-05-18 | E. I. Du Pont De Nemours And Company | Expression of herbicide metabolizing cytochromes |
JP3201411B2 (ja) * | 1990-06-15 | 2001-08-20 | メルシャン株式会社 | ビタミンd類の生物学的製造方法 |
DE69122016T2 (de) * | 1990-09-26 | 1997-04-30 | Sumitomo Chemical Co | Mitochondriales P450 |
-
1993
- 1993-06-24 WO PCT/JP1993/000856 patent/WO1994000576A1/ja not_active Application Discontinuation
- 1993-06-24 AU AU43574/93A patent/AU4357493A/en not_active Abandoned
- 1993-06-24 CA CA002138516A patent/CA2138516A1/en not_active Abandoned
- 1993-06-24 EP EP93913573A patent/EP0653490A4/en not_active Withdrawn
-
1994
- 1994-12-16 KR KR1019940704606A patent/KR950702247A/ko not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH02469A (ja) * | 1987-07-08 | 1990-01-05 | Taisho Pharmaceut Co Ltd | ビタミンd類の製造方法 |
JPH02231089A (ja) * | 1989-03-04 | 1990-09-13 | Taisho Pharmaceut Co Ltd | ビタミンd類の製造方法 |
Non-Patent Citations (2)
Title |
---|
FEBS Lett., Vol. 262 (1990), E. USUI et al., "Molecular Cloning of cDNA for Vitamin Ds 25-Hydroxylase from Rat Liver Mitochondria", p. 135-138. * |
See also references of EP0653490A4 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997004082A1 (en) * | 1995-07-19 | 1997-02-06 | Novo Nordisk A/S | Proteolytic enzymes derived from amycolata |
US5948746A (en) * | 1995-07-19 | 1999-09-07 | Novo Nordisk A/S | Proteolytic enzymes |
CN1131306C (zh) * | 1995-07-19 | 2003-12-17 | 诺沃奇梅兹有限公司 | 来源于无枝酸菌属的蛋白酶 |
Also Published As
Publication number | Publication date |
---|---|
EP0653490A1 (en) | 1995-05-17 |
KR950702247A (ko) | 1995-06-19 |
CA2138516A1 (en) | 1994-01-06 |
EP0653490A4 (en) | 1997-01-08 |
AU4357493A (en) | 1994-01-24 |
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