WO1992020226A1

WO1992020226A1 - Agricultural composition for controlling diseases caused by actinomycetes

Info

Publication number: WO1992020226A1
Application number: PCT/JP1992/000661
Authority: WO
Inventors: Naoki Akiyama; Tadao Hasegawa
Original assignee: Mitsubishikasei Polytec Co.
Priority date: 1991-05-24
Filing date: 1992-05-22
Publication date: 1992-11-26
Also published as: JPH04346903A

Abstract

An agricultural composition which is efficacious against plant diseases caused by actinomycetes and highly safe for the environment, which composition contains at least one member selected among the following polymers as the active ingredient: (A) a water-soluble nitrogenous polymer prepared by the reaction of at least one compound selected from the group consisting of epihalohydrin, alkylene halide, diepoxide and dihalogenoalkyl ether with an amine represented by general formula (I), wherein R1, R2, R3 and R4 represent each hydrogen, C1 to C3 linear alkyl or branched alkyl; A represents C1 to C6 linear or branched alkyl; and n represents an integer of 0 to 5, (B) an alkyleneimine polymer, and (C) a copolymer thereof.

Description

Specification

Agricultural composition for controlling disease caused by actinomycetes

Technical field

The present invention relates to an agricultural composition for controlling disease caused by actinomycetes, such as scab, which is caused by Masaru, sweet potato, sugar beet, radish, ginseng and the like and causes great damage. Background art

The scab disease caused by actinomycetes is caused by horse bell, sweet potato, sugar beet, radish, carrot, etc. and causes great damage. For example, in the case of potato, when the scab is infected, raised spots are formed on the surface of the tuber, which gradually expands and the surface becomes rough and rigid, giving a scab. For this reason, not only does the appearance become unsightly, the commercial value is reduced, but also the potatoes that have been infected with scabs have a reduced starch content. Give a big blow.

As a countermeasure against scab in potatoes at present, seeds such as potatoes are immersed in a mixture of an antibiotic such as streptomycin and a copper wettable powder, or immersed in a PCNB agent. Have been disinfected. In addition, the soil where scabs have developed is generally fumigated with Kokulpicrin.

However, the above-mentioned disinfection of seeds such as sweet potatoes is not enough as a countermeasure against scab and has almost no effect of disinfection treatment, but there is no other effective method and it is carried out with a restfulness Is the actual situation. On the other hand, soil fumigation with clopicrin has many problems because chlorpicrin is extremely toxic and volatile. First, the soil is covered with polyethylene film and fumigated. Chlorpicrin evaporates outside during fumigation, irritating the eyes and bronchi, and harms the human body. For this reason, during the fumigation and for several days after the fumigation, there was the inconvenience that people living in the surrounding area had to stay at hotels and live away from the fumigation site, and the animals near the fumigation site became toxic. There is also a report example. In addition, the ecological balance is destroyed in order to eliminate microorganisms in the soil, and potatoes are susceptible to disease after application, and contaminated potatoes are planted after fumigation with Kokulpicrin. There have been reports of scab disease occurring frequently (Agriculture of the Month, p. 79, April [19989]). In addition, once the disease occurs, there is a major problem in that the following year, the disease cannot be prevented by that means.

As mentioned above, there are major problems with the countermeasures against scabs using conventional drugs, but since there is no appropriate alternative, the above drugs must be used. Although it is outside the range of 5.5 to 6.5, which is the appropriate cultivation condition by Masaru, the pH range of the soil is adjusted to 4 by removing the PH range within the appropriate growth range of the scab disease pathogen. It is cultivated with 5 or less. However, this method significantly reduces the yield and reduces the yield by less than half. In some cases, they are cultivated about once every three years without continuous production by Masuzu. However, this method cannot be applied to single crop areas.

The present invention has been made in view of the above circumstances, and an object of the present invention is to provide an agent effective against plant diseases caused by actinomycetes represented by scabs. To provide the above-mentioned drug which is safe and easy to apply to the surrounding environment without causing phytotoxicity.4> Disclosure of the Invention That is, the present invention

(A) at least one compound selected from the group consisting of epihalohydrin, alkylenedihalide, epoxide, and dihalogenoalkyl ether;

R ₂

_{(R!, R 2, R} 3 and R ₄ are hydrogen or Chokukusaria Rui branched alkyl group having a carbon. 1 to 3, A is linear or branched chain alkyl group having from 1 to 6 carbon, n represents 0 An integer from 5 to 5)

A nitrogen-containing water-soluble polymer obtained by reaction with an amine represented by

(B) an alkylene imide polymer, and

(C) these copolymers

The present invention relates to an agricultural composition for controlling a disease caused by actinomycetes, which comprises one or more selected from the group as an active ingredient. General formula which is a raw material of the polymer or copolymer which is the active ingredient of the present invention

R _a R ₃

R ₂

(R j, R ₂ , R. and R ₄ are hydrogen or a linear or branched alkyl group having 1 to 3 carbon atoms, A is a linear or branched alkyl group having 1 to 6 carbon atoms, and n is 0 An integer from 5 to 5)

For monoalkylamines (n = 0), Monamine, methylamine, dimethylamine, getylamine, zipperamine, methylethylamine, methylpropylamine, ethylpropylamine, etc., and alkylenepolyamine (n = 1 ), Ethylenediamine, N, N-dimethylethylenediamine, N ,， '-dimethylethylenediamine, Ν, Ν-jethylethylenediamine, Ν, Ν'-ethylethyldiamine , Propylenediamine, Ν, Ν—dimethylpropylenediamine, Ν, Ν, Ν ′, Ν, tetramethylethylenediamine, polyalkylenepolyamine (η = 2 to In 5), diethylenetriamine, triethylenetriamine, triethylenetetramine, and the like can be mentioned. These amines can be used together within the above groups and between groups.

The epihalohydrin which reacts with the amine is represented by the general formula:

CK ₂ -CK-CH,-X

\ /

\ (X is halogen)

, And the halogen may be any of fluorine, chlorine, bromine and iodine, but is preferably epichlorohydrin for economic reasons.

As the alkylene dihalide, a general formula,

X-B-X

(B is a 1-20 linear or branched alkylene group, X is a halogen)

The following are used. Specifically, dichloroethane, dichloroprononone, dichlorobutane, dichlorohexane, dibromoethane, and promochloroproha. , Dibromopropane, dibutenemohexane, dichlorononane and the like. In particular, 1,3-dichloropropane Are preferred due to their reactivity with amines and economic reasons.

In addition, the general formula,

(D is absent, or a linear or branched alkylene group having 1 to 4 carbon atoms, or a group represented by one (CH ₂ -0-CH ₂ ) _m , where m is 1 to 4)

The following are specifically used: 1,3-butadiene japoxide, 1,4-pentadiene japoxide, 1,5-hexadienpoxide, 1,6 — ぺ Butadiene japoxide, 1,7-year-old kuta jenpoxide, ethylene glycol glycidyl ether, triethylene dalicydyl ether, etc. The dihalogeno alkyl ether is represented by the general formula:

_{_{X- (R 5 - 0) &}} -Rg -X

(R ₅ and R g are a linear or branched alkylene group having 1 to 4 carbon atoms, X is a halogen, and d is an integer of 1 to 12)

Is used. Specifically, when ^ = 1, 2,2′—dichloroethyl ether, 2,2, dibromoethyl ether, 3,3, dichloromethyl propyl ether, 2,3-dichloro mouth propyl ether, 4,4'-dichlorobutyl ether, 4,4'-dibromobutylbutyl ether and the like. In the case of ^^ 2, the degree of polymerization is up to 10 Examples include dihalogenoethyl ether which can be obtained by a dehydration reaction or the like from an ethylene oxide polymer and an alkyl alcohol halide. In particular, 2,2, -dichloroethyl ether (hereinafter referred to as dichloroethyl ether) is preferred at least for economic reasons. The above-mentioned ephalohydrin, alkylenedihalide, japoxide and dihalogenoalkyl ether may be used alone or in combination of two or more kinds for each component. In the case of mixing two or more kinds, there is no particular limitation as long as the sum of the number of moles of the mixture is substantially equal to the number of moles of the amide.

In order to react at least one compound selected from the group consisting of epihalohydrin, alkylenedihalide, gepoxide and dihalogenoalkyl ether with amine, epihalohydrin, alkylenedihalide, A closed type reaction vessel equipped with a stirrer was prepared by making the sum of the moles of one compound selected from the group consisting of the epoxides and dihalogenoalkyl ethers, or the sum of the moles of the mixture approximately equal to the moles of the amine. The reaction is preferably performed under an inert gas atmosphere with the temperature inside the reaction vessel in the range of 30 to 100.

More specifically, a 20 to 70% aqueous solution of the above-described amine is charged into a closed reaction vessel equipped with a stirrer, a reflux condenser, a thermometer, and the like, and the atmosphere in the vessel is replaced with nitrogen gas. Then, while stirring, the temperature in the reaction vessel is adjusted to a predetermined temperature, and a single compound selected from the group consisting of epihalohydrin, alkylenedihalide, gepoxide, and dihalogenoalkyl ether, or a mixture thereof is continuously fed. It may be added in a targeted or batch manner. However, 100% amine can be used as an amine. Water is mainly used as the reaction solvent, but methyl alcohol, ethyl alcohol or dimethylformamide may be used in consideration of the solubility of the raw materials and the reaction products.

The water-soluble polymer thus obtained is further processed to convert a halogen (derived from epihalohydrin, alkylenedihalide and dihalogenoalkyl ether) as a counterion into another anion, for example, may be used instead of so ₄ — or remove counter ions The leverage polymer may be a hydroxide. These water-soluble polymers can be made into a solid by removing the solvent (usually water), or can be produced as an aqueous solution as it is, or by diluting or concentrating as needed. Can also be used.

The alkyleneimine polymer has the general formula:

(R ₇ is an alkylene group, preferably a straight-chain or branched-chain alkylene group having 1 to 8 carbon atoms. A plurality of R ₇ may be the same or different. The degree of polymerization of is shown.)

A polymer represented by the formula is used, but these aldimylene polymers do not necessarily have to have a completely linear structure as shown in the above general formula, and may have a branch. No problem. Also, a quaternary salt of an alkyleneimine polymer obtained by adding hydrochloric acid or the like to the alkyleneimine polymer may be used.

Examples of such an alkyleneimine polymer include an ethyleneimine polymer, a propyleneimine polymer, a trimethyleneimine polymer, a tetramethyleneimine polymer, and a hexamethyleneimine polymer. Union and their copolymers, and mixtures of at least two of them o

Of these, polymers of ethyleneimine are particularly preferred for economic reasons. This is generally obtained by ring-opening polymerization using ethyleneimine and cation catalysts, but also other reactions such as the reaction of ethyleneimine with ethylenedihalide or ammonia, the condensation reaction of ethanolamine, etc. Can also be obtained.

The above amine reactant and alkyleneimine polymer are dissolved in 2 mol / ^ of KBr aqueous solution and measured and calculated. The intrinsic viscosity at 25 ° C is calculated. By [7?], The magnitude of the molecular weight can be determined. The limiting viscosity [7?] Is less than 1 Zg, preferably less than 0.6 dZg, more preferably less than 0.4 d, since the effect on actinomycetes is stronger in the lower molecular weight range. ^ Z g or less is desirable, but is not particularly limited to that range.

Further, the active ingredient of the present invention can be used not only in each of the above polymers but also in a copolymer. That is, a copolymer obtained by reacting at least one compound selected from the group consisting of ephalohydrin, alkylenedihalide, epoxide, and dihalogenoalkyl ether with an amine represented by the above general formula, alkylene imide It may be a copolymer, or a copolymer of the above-mentioned polymer or the above-mentioned copolymer and the above-mentioned alkylene imide polymer.

Next, a method for applying the composition of the present invention will be described.

When cultivating a crop susceptible to actinomycetes, such as potato, such as potato, the active ingredient of the present invention alone or in the soil at an appropriate time from 1 to 2 months before the planting of the crop and immediately before planting. The mixture is diluted with water and other solvents to 110-: LZ 100,000 Diluted as it is, or mixed with other pesticides, fertilizers, etc., and sprayed or dropped Mix. The application rate of the present composition is preferably 0.2 to 20 kg per 10 ares, but is not limited to this range.

When spraying on soil, the aqueous solution of the composition is mixed relatively thickly with the soil itself, or absorbed into formic lime, calcium carbonate, chemical fertilizer, and other inorganic diatomaceous earth zeolites. After that, there is a method of mixing with the cultivation soil.

As an application method during cultivation and cultivation, there is a method of spraying the above aqueous solution, but holes are made at the time of spraying because it is easily absorbed only on the soil surface It is better to put it into the soil using a pipe called pipe injection. The above application range is economically appropriate. A mixture of formic lime etc. may be used in the same manner as above, but it is more effective to put it in the soil.

Furthermore, the seeds or seed potatoes of the crop are immersed or applied to a solution obtained by diluting the active ingredient of the present invention to 1Z10 to 1Z10,000 with a solvent such as water, or a mixture thereof with other pesticides. Then, bringing the seeds and the like into sufficient contact with the drug is also effective in preventing the disease.

The use ratio of the present composition in soil admixture or seed disinfection is not limited to a limited range, but a small amount has a sufficient effect, so that a use amount of about 1/50 to 1Z1000 is appropriate.

When the composition of the present invention is applied in this manner, plant scabs and other plant diseases caused by actinomycetes can be easily suppressed, and the yield of crops increases. In addition, since it is not necessary to cultivate under conditions that are not suitable for cultivation (pH, etc.) in order to avoid disease as in the past, the yield can be increased in this respect, and continuous cultivation is possible. .

Such an effect could not be obtained at all with the conventional drugs, and the composition of the present invention can be said to be completely breakthrough against diseases caused by actinomycetes.o

Further, the present invention also has the following effects, and is extremely high in practical value.

(1) Since the composition of the present invention is substantially non-volatile, there is no need to pay attention to the surrounding safety when using the composition.

(2) The composition of the present invention has no effect on the germination rate, leaf bud rate, etc. of the crop, and has no adverse effects on cultivation.

(3) The composition of the present invention is extremely safe, non-irritating to warm-blooded animals, and extremely simple to use.

(4) The composition of the present invention is substantially harmful to useful soil microorganisms. As it does not lose the balance of soil microorganisms, crops can be grown using normal cultivation methods.

The active ingredient of the composition of the present invention was known as a settling accelerator or a flocculant, but such an effect on agricultural crops was completely unexpected.

As described above, the effects of the composition of the present invention have been described for scabs occurring in crops such as potatoes such as potatoes, potatoes, radishes, and carrots. Diseases caused by actinomycetes are not limited to scabs. Can be controlled.

In recent years, there are various cultivation methods for crops.Therefore, not only in soil cultivation but also in hydroponics or gravel cultivation, for example, disinfect seeds, add a small amount of water to water supply, circulating water, etc. This can prevent the occurrence of diseases caused by actinomycetes as pathogens. BEST MODE FOR CARRYING OUT THE INVENTION

Hereinafter, the present invention will be described specifically with reference to examples. In the following examples, in Example 1, effects on actinomycetes Streptomyces (Streptomyces) and other microorganisms, in Example 2, results from pot tests, and in Example 3, experiments on experimental farms In Example 4, the effect of seed disinfection will be described.

First, a method for preparing a water-soluble polymer used in each example and a method for preparing a mixture containing a water-soluble polymer will be described.

(1) Sample A

Epiclorhydrin was added to a 50% aqueous dimethylamine solution while maintaining the reaction temperature at 85 ° C, and the mixture was stirred and mixed until the molar ratio of dimethylamine and epichlorohydrin became 1: 1. The mixed reaction composition (composition having an intrinsic viscosity [??] of 0.11 dZg measured in 2 mol of KBr aqueous solution) was diluted with water to a purity of 50% by weight. The sample obtained was designated as Sample A.

(2) Sample B

A 40% aqueous solution of a mixture of dimethylamine and ammonia prepared in a molar ratio of 1: 0.1 in advance, and epichlorohydrin and propylene dichloride prepared in a molar ratio of 1: 0.5 in advance. A mixture of epichlorohydrin and propylene dichloride in a 40% aqueous solution of the amide mixture was maintained at a reaction temperature of 90 ° C until the molar ratio of the mixture of chlorides was almost 1: 1. While stirring and mixing, and diluting the obtained mixed reaction composition (a composition having an intrinsic viscosity [7?] Of 0.16 dZg as measured with a 2 mol / KBr aqueous solution of 0.16 dZg) with water, The purity was adjusted to 50% by weight.

(3) Sample C

A 50% aqueous solution of a mixture of dimethylamine and Ν, Ν, Ν, ，, Ν, ー -tetramethylethylenediamine prepared in a molar ratio of 1: 0.3 in advance, and a molar ratio of 0,5 : Ethylene glycol glycidyl ether and dichloroethyl ether in an aqueous mixed amine solution until the molar ratio of the mixture of ethylene glycol glycidyl ether and dichloroethyl ether prepared in the ratio of 1: 1 becomes approximately 1: 1. The mixture was added while maintaining the reaction temperature at 70 ° C, and mixed by stirring. The resulting mixed reaction composition (intrinsic viscosity [??] measured with a 2 mol / ^ KBr aqueous solution) was 0.33. Was diluted with water to a purity of 50% by weight.

(4) Sample D

A 50% aqueous solution of a mixture of dimethylamine and N, N, N,, Ν, 一, 1-tetramethylethylenediamine prepared in a molar ratio of 1: 0.3 in advance, and a molar ratio of 1: 1 The molar ratio of the mixture of epichlorohydrin and propylene dichloride prepared at a ratio of approximately 1: 1 The mixture of epichlorohydrin and propylene dichloride was added to the mixed amine aqueous solution while maintaining the reaction temperature at 80 ° C, and mixed by stirring. A sample having a limiting viscosity force of 0.23 di / g measured with an aqueous KBr solution of the above) was diluted with water to obtain a sample having a purity of 50% by weight, which was designated as Sample D.

(5) Sample E

Commercially available polyethyleneimine with branched structure containing primary, secondary and tertiary amino nitrogen with the following structure (Intrinsic viscosity measured with 2 mol / ^ KBr aqueous solution [? 7] Force 0. A sample having a purity of 50% by weight was diluted with water to obtain a sample E.

H ₂ -rCK ₂ -CHz-N-¾-rCH ₂ -CK ₂ -NH-} 7-

CH ₂

One N—

(6) Sample F

This sample A was sprayed and mixed on calcium carbonate for soil admixture, dried at 60 ° C, and a sample of this composition: calcium carbonate 1: 9 was prepared, and this sample F was obtained.

(7) Sample G

Intrinsic viscosity determined with 2 moles of KBr aqueous solution [7?] Force << 1.12 di dimethylamine-epichlorohydrin copolymer was obtained, which was diluted with water to a purity of 50% by weight. Was used as Sample G.

Example 1. Effects on actinomycetes and other microorganisms

Streptomyces MAFF 03-01524 was obtained from the Agricultural and Biological Resources Institute, Ministry of Agriculture, Forestry and Forestry, and the bacteria were Staphylococcus aureus ATCC 6538 and Pseudodomo. Using Pseudomonas aeruginosa P2, the fungus Aureobas idium H 2627 and Candida albicans IF 01594, MIC (minimum inhibitory concentration) and MCC (minimum sterilization) in the medium Concentration) was measured.

Actinomycetes: Yeast extract and starch medium (2 g yeast extract, 10 g soluble starch, 1.0 mi distilled water, pH = 7.0) were steam sterilized at 115 ° C for 20 minutes. Thereafter, the above actinomycetes were cultured at 25 ° C for 5 days. Bacteria: Pre-cultured in 5 m of SCD pros at 35 ° C. for 24 hours and adjusted to a bacterial count of about 10 ⁶ / m 2 was used as a test bacterial solution.

Yeast: GP broth 5 precultured for 48 hours at 3 5 ° C in m, prepared in as saline give a cell number 1 X 1 0 ⁶ Zm, was tested bacterial solution. Mold: Potato dextroses were inoculated on slant medium and pre-cultured at 30 ° C for 10 days. Its slope to 0.0 5% preparative window I over emissions (Twe en) Put 8 0 saline, and adjusted to be spore number 1 XI 0 ⁶ / m &, and a test bacterial solution.

For the actinomycetes, bacteria, and fungi, MIC and MCC were measured by the liquid medium dilution method.

Using the medium described above, a predetermined amount of each of the samples A to E was added to prepare a two-fold dilution series. The volume of the medium was 2 m. Each test tube is inoculated with 0.1 μm of the test bacterial solution, cultured for 5 days at 25 ° C for actinomycetes, cultured for 48 hours at 35 ° C for bacteria and yeast, and for 7 days at 30 ° C for mold. After culturing, the MIC value was determined based on the minimum sample concentration in the test tube where no turbidity was visually observed.

In addition, from each test tube that does not show turbidity, inoculate one loopful of the loop into each of the culture media without sample, and culture the actinomycetes at 25 ° C for 5 days. Yeast was cultured at 35 for 48 hours, and mold was cultured at 30 ° C for 7 days, and the MCC value was determined based on the minimum sample concentration in a test tube in which no turbidity was observed.

The results are shown in Table 1.

【table 1】

Yugo 1

MIC (Value unit is mg / 1 pure); CC sample A sample B sample. Trial D

Streptomyces MIC 20 20 80 40 20 i

MCC 20 40 80 40 40 \

Stapn lococcus MIC 750 1,500 3,000 1,500 1,500 '■ aureus MCC 1,500 3,000 3,000 3,000 3,000;

Pseuaomonas MIC 3,000 3,000 6,000 3,000 3,000 I aeruginosa MCC 3 3,000 3,000 5,000 3,000 3,000:

Aureobas Gium MIC 50,000 left § Same as left Same as left §

vrr two _c

Candida MIC 50,000 Same as left Same as left

Growth with albicans MCC Example 2. Pot test

After mixing granular fertilizer and calcium carbonate (0.3% by weight) into the soil contaminated with scab, 7 kg of each was placed in a pot with a diameter of 30 cm. Excluding sample F, pure 1Z20 The aqueous solutions of the above 0 samples were mixed with the soil while spraying the soil in the pot, and each sample was prepared so as to have a pure content of 60 ppm with respect to the soil. The pots containing each sample were numbered from Pot A to Pot G in the same way as the sample name. In addition, 8.4 g of Sample F was added to the above 7 kg of soil, mixed well, and this pot was named Pot F.

In addition, a sample without any sample was prepared, and this pot was used as a control.

In addition, each pot was prepared by preparing three identical potions (n = 3). The pots prepared as described above were left in place for 2 weeks, and then potatoes were disinfected with streptomycin and copper wettable powder. Was planted as a seed potato (two kinds of Yutaka).

The pots were laid outdoors and grown, and 70 days after planting, the tubers were dug up and examined for tubers weighing about 15 g or more. The diseased potatoes were also regarded as diseased potatoes, and the diseased potatoes were divided by the harvest amount, and the lesion area ratio (the disease area divided by the harvested potato amount) was determined.

The results are shown in Table 2.

[Table 2]

Mouth

Pot name Diseased tuber rate (%) Lesion area rate (%)

Contour Nore 84.1 35.6

Pot A 5 2.8

Pot B 50.7 6.6

Pot C 61.6 10.2

Pot D \ '\. 26.6

Pot E 53. 0 7. 9

Not found F 42. 3 2. 9

Pot G 12.3 25.8 le Example 3. Evaluation in experimental farm

The granular compound fertilizer and calcium carbonate (0. Triple mass%) were mixed to flare section of scab, and divided into 6 m ^2, and the experimental group.

The experimental plots were divided into 1 to 21 plots, and three plots were randomly selected from the plots. The whole plot was designated as test plots 1 to 7, and the above three plots were tested under the same conditions.

Test zone 1 was used as a control, and the ridges were raised in the same manner as in the general cultivation method. .

In test plot 2, sample A was diluted with water to make an aqueous solution with a pure content of 1Z200, and cultivated while spraying 720 g per plot, so that sample A was mixed with the soil. After three weeks, the ridges were raised and planted in the same manner as in Test Zone 1.

In test plot 3, dilute sample A with water to make an aqueous solution with a pure content of 1Z200, plow it while spraying 144 g per plot, and mix sample A with soil. The test was performed in the same manner as in Test Zone 2.

Test plot 4 was the same as test plot 1. However, two weeks after planting, the aqueous solution used in the test area 2 was injected into a tube (insert the tube into the soil, and pour the aqueous solution near the place where the horse was planted by Masaru). The dose is 144 g.

In test plot 5, sample D was diluted with water to make an aqueous solution with a pure content of 1Z200, and cultivated while spraying 720 g per plot in the test plot to mix sample D into the soil. After three weeks, the ridges were raised and planted in the same manner as in Test Zone 1.

In test plot 6, 900 g of sample F was added to the soil per test plot, mixed well, and after 3 weeks, the ridges were raised and planted in the same manner as test plot 1. In test plot 7, sample G was diluted with water to make an aqueous solution with a purity of 1200, and cultivated while spraying 720 g per plot to mix sample G in the soil. Same as 5.

In each test plot, two weeks after planting, ridges were covered with polyethylene sheet and mulched. Three months after planting, the tubers were dug up and examined for tubers weighing about 20 g or more. And the lesion area ratio (value obtained by dividing the lesion area by the amount of harvested potato).

The results are shown in Table 3.

[Table 3]

Result 3.

： Test plot unit Total tuber number ′ Diseased tuber number (%) ： Lesion area rate (%) i

1 99.1 39.7!

2 149.0 62.9 4.1!

57.8 3.9!

4. 152.6 74.6 13.2 i

Δ ί 155.4 83.8 17.6!

6 155.0 63.0 4.9 ¹

i

94. 1 26.7 1 Example 4. Example of seed disinfection

The field was disinfected with chlorpicrin at a ratio of 30 / 10a, and each 6 m2'-division was divided into experimental plots.

The experimental plot is divided into 121 plots, and three plots are randomly selected from the plots, and the whole is designated as test plot 17 plots. Was

Test zone 1 was set as a control (target zone), and ridges were raised in the same manner as in the general cultivation method. In the plot, the so-called “harvest” was harvested from the field where scabs occurred and no lesions were visually observed. Potatoes (apparently healthy potatoes) (two types of Yutaka) were planted as seed potatoes.

In test plot 2, the same potato as in test plot 1 which was immersed for about 3 seconds in 1 aqueous solution of sample A diluted to 1100 with water for 1 second after disinfection of seeds was used as seed potato. Planting was performed in the same manner as in test plot 1.

Test plots 3, 4, 5, 6, and 7 were performed in the same manner as test plot 2, except that samples B, C, D, E, and G were used for seed disinfection, respectively.

Three months after planting, the tubers were dug up, and the tuber weight of about 20 g or more, the total tuber count and the diseased tuber rate (disease of about 2 mm. (Divided by the yield) ¾ ·

The results are shown in Table 4.

[Table 4]

Result 4

Test plot unit Total tuber count Diseased tuber rate ()

1 35. 7 71. 2

2 38. 7 7.4

3 37. 0 11.7

4 38. 3 34. 3

5 38. 5 27. 2 '

6 3T. 8 20. 6

35.0 58.0

⁷ I 9 Industrial applicability

As described above, the composition of the present invention has an excellent control effect on the disease caused by actinomycetes on plants represented by scab, and is not as toxic as conventional drugs against scab. Since it is not volatile, it is highly safe to the surrounding environment and its application method is simple. Furthermore, it does not cause phytotoxicity to cultivated crops.

Claims

The scope of the claims

1. (A) at least one compound selected from the group consisting of epihalohydrin, alkylenedihalide, gepoxide, and dihalogenoalkyl ether;

R. R ₃ R ₂

(R j, R ₂ , R ₃ and R ₄ are hydrogen or a linear or branched alkyl group having 1 to 3 carbon atoms, A is a linear or branched alkyl group having 1 to 6 carbon atoms, and n is 0 An integer from 5 to 5)

A nitrogen-containing water-soluble polymer obtained by a reaction with an amine represented by the formula:

(B) an alkyleneimine polymer, and

(C) these copolymers

An agricultural composition for controlling diseases caused by actinomycetes, which comprises one or more selected from the group as an active ingredient.