JP4909518B2 - Control method of continuous cropping failure - Google Patents

Description

  The present invention relates to a method for controlling continuous cropping damage to root vegetables, and more particularly, at least field analysis of soil before planting root vegetables, and antagonizing root vegetable pathogens in relation to the analysis value. The present invention relates to a system that controls the continuous cropping disorder of root vegetables by controlling the application amount of effective microbial materials.

  There have been many studies on microbial materials that actively use microorganisms that have an antagonistic action against plant pathogens (antagonist microorganisms, hereinafter referred to as antagonistic bacteria) to control disease. There are very few success stories at the farm level. Here, an antagonistic bacterium is a microorganism that suppresses the growth and activity of a specific bacterium, and can suppress the growth and activity of a pathogenic bacterium, and as a result, can reduce a disease to a plant. In particular, in the current situation where there is a call to shift to organic farming that reduces the use of pesticides that are concerned about safety and environmental destruction, and ecologically utilized (environmental conservation) farming such as reduced or non-pesticide farming It is no exaggeration to say that the technology of microbial materials that function as an agrochemical using such antagonistic bacteria is a dream technology, and its development is eagerly desired.

  However, as described above, although various studies have been made, there are few successful examples on actual farms. The reason for this is that there are a great variety of soil bacteria in the farm soil. In such farms, the state of the antagonistic culture solution (see, for example, Patent Document 1), the culture material, Even if a large amount of antagonistic bacteria (see, for example, Patent Document 2) in which a large amount of antagonistic bacteria were propagated at a time, they lost the competition with other microorganisms (those that have settled in the soil). This is probably because antagonistic bacteria cannot often take root in the soil.

  On the other hand, when a dry-immobilized microorganism is actually used on a farm using a technique for obtaining a dry-immobilized microorganism (see, for example, Patent Documents 3 and 4), antagonistic bacteria are caused by moisture in the soil. It has been disclosed that it can recover the life activity of and can effectively function as a microbial material (see, for example, Patent Document 5).

  However, since the microbial materials as described above obtain the effect of reducing diseases against plants using the life activity of antagonistic bacteria, in order to exert a high effect on application to plants in actual fields In addition to developing microbial materials that exhibit high antagonism against the pathogenic fungi of the target plant, in an actual field that is considered to be in various environments, the microbial material is In order to maintain high efficacy for a long time with more certainty, it is considered that a comprehensive study including application methods is necessary.

  In particular, continuous cropping failure, which is considered to be caused by a large amount of use of agricultural chemicals and chemical fertilizers, is serious, and in some fields it is difficult to grow the next crop unless it is fallowed for several years. Stable effects of microbial materials are exerted even in fields with such continuous cropping failures, healthy crops can be obtained with good harvest rate, and continuous cropping failures are suppressed by reducing the use of agricultural chemicals and chemical fertilizers. It would be very useful if we could move on to environmentally friendly agriculture.

Japanese Examined Patent Publication No. 7-101815 Japanese Patent Publication No. 6-192028 Japanese Patent Publication No. 4-48436 Japanese Examined Patent Publication No. 6-73451 JP 2002-308714 A

  Therefore, the object of the present invention is to take root vegetables as specific plants, develop microbial materials effective for root vegetables, and at the same time, apply them to fields that suffer from continuous cropping failures when applied to actual fields. In some cases, healthy crops can be obtained stably at a high yield, and the use of pesticides and chemical fertilizers can be reduced, reducing the occurrence of continuous cropping failures and promoting environmentally friendly agriculture. It is to provide a technology that can realize the transition.

  The above object is achieved by the present invention described below. That is, the present invention [1] performs a soil analysis of a field before planting root vegetables, measures the density of common scabs in the field by the amount of the nec-1 gene, and obtains the obtained nec-1 gene. It is a control method for continuous cropping failure, characterized in that the amount of microbial material applied to the field is controlled by the amount.

In addition, a preferred form of the present invention is [2] the microbial material is at least a solid nutrient medium and Trichoderma asperellum F288 strain (deposit number: NITE P-53) in the range of 10 ³ to 10 ⁶ cells per gram of microbial material. ) Is a continuous crop failure control method according to the above [1].

  Furthermore, the following are mentioned as a preferable form of this invention. [3] Further, in the soil analysis, the phosphoric acid concentration in the field is measured, and in the case of a field determined to have a high phosphoric acid concentration, the fertilizer is not fertilized [1] or [ [2] The method for controlling a continuous cropping failure according to [2]. [4] The method for controlling a continuous cropping disorder according to the above [1] to [3], further comprising applying ammonium sulfate as a fertilizer. [5] When the root vegetable to be planted is a seed pod, the presence or absence of the scab on the seed pod is analyzed in advance. The method for controlling a continuous cropping disorder according to any one of the above [1] to [4], wherein attaching is performed. [6] Further, in the soil analysis, the pH of the soil is measured, and when the pH value of the soil is higher than 5.5, ammonium sulfate is applied to the field, and the microbial material is applied in that state [1] The control method of the continuous cropping disorder in any one of-[5].

  According to the present invention, a microbial material that has an antagonistic action against root vegetables pathogens is accurately applied according to the state of the field and further the state of the seed pod, so that a healthy crop can be stably harvested. It is possible to provide a control system for continuous cropping failures that can be obtained well and that has high practical value and can reduce continuous cropping failures by reducing the use of agricultural chemicals and chemical fertilizers.

  Next, the present invention will be described in detail with reference to preferred embodiments. As a result of intensive studies to solve the above-described problems of the prior art, the present inventors are fixed in a state where the activity is maintained, and swell with moisture in the soil during use to recover the life activity of the antagonistic bacteria. And we found Trichoderma asperellum F288 strain, a novel antagonistic microorganism that can be used effectively for microbial materials that exhibit high functions as so-called pesticides. Further, as a result of intensive studies on optimum conditions for applying the microbial material to an actual field, applying the microbial material according to at least the soil environment, and further applying the microbial material in a state in which the soil environment is prepared It is important to adopt such an application system, and it has been found that healthy crops can be obtained stably and with a good harvest rate even in fields where continuous cropping failures occur. It came.

First, the Trichoderma asperellum F288 strain (deposit number: NITE P-53) that characterizes the present invention will be described. The microorganism is a fungus belonging to the genus Trichoderma. As a result of intensive studies on the novel microorganism, the present microorganism has a high antagonism against Streptomyces s cabies, which is a pathogen of potato scab. I found something. Further, according to further studies by the present inventors, this microorganism exhibits a high antagonistic action against root pathogen pathogens as well as potatoes. Furthermore, the microbial material comprising at least the solid nutrient medium is fixed in a state where the activity is maintained, and when used, it swells with moisture in the soil and recovers its life activity, and the above-described antagonistic action is high. It can be used effectively as a so-called pesticide.

The microbial material used in the present invention contains the Trichoderma asperellum F288 strain in a range of about 10 ³ to 10 ⁶ cells per gram of the finally obtained microbial material. In preparing the microbial material to be used in the present invention, it is preferable to use Trichoderma asperellum F288 strain that has been cultivated purely microbiologically in advance. As a method for culturing antagonistic bacteria, a conventionally known microbiologically pure culture method may be used. In particular, it is preferable to use a solid culture method using an organic plant residue discharged from a food factory. Specifically, for example, a method of culturing using a solid nutrient medium composed of organic plant residues such as bran, rice bran, okara and red bean residue is preferable.

  The microbial material used in the present invention can be obtained, for example, by further mixing Trichoderma asperellum F288 strain previously cultured by a solid culture method or the like as described above with a solid nutrient medium, in particular, Trichoderma asperellum. A solid medium used for culturing the F288 strain and a solid nutrient medium to be further mixed are preferably the same.

  The microbial material used in the present invention may contain a vermiculite material which is a clay mineral in addition to the above. Since vermiculite is porous, it is a good fixing material for antagonistic bacteria. The above-described microbial material containing vermiculite is a product that maintains a certain quality and maintains its effect even though it utilizes the life activity of microorganisms. The present inventors, for the reason, vermiculite in the material is as if the surface of the organic plant residue such as bran is coated, the organic plant residue constituting the microbial material is It is thought that it is possible to effectively suppress the feeding of other various microorganisms present in the soil, and to provide an environment in which antagonistic bacteria contained in the microbial material can selectively grow and proliferate. ing.

  Specific examples of easy-to-handle microbial materials that can be used in the present invention include a mixture containing Trichoderma asperellum F288 strain cultured in a solid medium and an organic plant residue such as wheat bran, or a mixture thereof. A mixture obtained by granulating or pelletizing a mixture of clay minerals such as vermiculite material may be used. These microbial materials are fixed in a state where the above microorganisms maintain their activity. At the same time, when microbial materials are sown together with crop seedlings, seeds and seed pods, the microorganisms swell and are good in the soil. It can grow and exhibits its high antagonism. The disease-reducing effect on plants by the antagonistic bacteria is stable and sustained for a long time.

  The above microbial material includes Trichoderma asperellum F288 strain and a solid nutrient medium composed of organic plant residues selected from, for example, bran, rice bran, okara, and red bean residue. In such a case, the antagonistic bacteria having a sufficient substrate (food) of the antagonistic bacteria are likely to grow, and the effect is maintained for a longer period of time. According to the study by the present inventors, the microbial material particularly using the bran is contained in the soil, and even when it is in a state of competing with other microorganisms living in the soil, the propagation of antagonistic bacteria In addition, the effect of the antagonistic bacteria is sustained over a long period of time.

  The granular microbial material can be produced by a known method using a tumbling granulator or the like using raw materials such as a solid medium in which Trichoderma asperellum F288 strain is cultured and a solid nutrient medium. Specifically, a wet powder raw material obtained by adding water to the above-mentioned powdery raw material is agglomerated and granulated by moving it with a granulator by rolling, vibration, stirring, etc. Examples thereof include a granulation method utilizing an agglomeration phenomenon caused by spraying an aggregating binder or water onto the dry powder in the layer.

  Examples of the aggregating binder used in the above case include starches such as corn starch, wheat flour, processed starch, CMC (carboxymethylcellulose), polyvinyl alcohol (Poval), which have strong caking power / water supply capacity / water retention capacity. ) Etc. can be used. In the case of using these binders, the binder may be mixed in the powder raw material, and water may be sprayed while applying rotation or vibration to the mixture, or the binder may be applied while rotating or vibrating the powder raw material. An aqueous solution may be sprayed.

  If the raw material mixture is formed into granules or pellets as described above and then dried, a solid microbial material product with high strength can be obtained. As the drying temperature, it is preferable to perform dehumidification and drying treatment at a slightly higher ordinary temperature, for example, a temperature of 40 ° C. or lower. A specific drying temperature may be appropriately determined according to the temperature resistance of the microorganism.

  When the above microbial material is sown in the field, it swells in contact with moisture in the soil, and by swelling, the Trichoderma asperellum F288 strain that has been fixed in the solid nutrient medium and vermiculite regains activity, In the field soil, it exerts an antagonism that suppresses the growth and activity of pathogenic bacteria on the crops, and is effective in reducing diseases against the crops. This is because organic plant residues, which are solid nutrient media, coexist around the strain that has regained its activity in the soil, and the substrate of the antagonistic bacteria (food) is sufficiently present. Even when competing with a large amount of microorganisms such as pathogenic bacteria, it is considered that the antagonistic bacteria grew well without being killed, and the antagonistic bacteria were always able to form a state in the soil. Pellet-like microbial materials not only have sufficient strength and are easy to handle when going to the farm, but also swell gradually from the surface to the inside due to moisture in the soil, so they are simply mixed Compared with the thing and granular thing, an antagonistic action can be maintained over a long period of time.

  In the present invention, in view of the above characteristics, the microorganism material to be used may be selected according to the target plant by selecting the raw material and selecting the shape (whether or not to be granulated or pelletized, or the size). For example, a microbial material formed into a granular shape or a pellet shape is excellent in handleability as compared with a material obtained by simply mixing the above raw materials. Also, if the microbial material is in the form of pellets, the effect of antagonistic bacteria will last for a longer time in the applied soil, so use it for plants that need to maintain the effect. Is preferred.

  The method according to the present invention is characterized in that the above-mentioned microbial material is used for cultivation of root vegetables. In this case, the soil analysis of the field is performed before planting, and the common scab in the field The amount of microbial material applied to the field is controlled as follows according to the amount of the nec-1 gene.

  In the present invention, as a specific method for measuring the density of scab pathogen by the amount of nec-1 gene, any analysis method may be used as long as the density of scab pathogen in the soil can be quantified, and is not limited. . As a method for measuring the amount of nec-1 gene in soil, a competitive QP-PCR method using an internal standard gene can be used. In this method, nucleic acid extracted from soil and an internal standard gene at a known concentration are competitively PCR amplified using primers for detecting the nc-1 gene, and fluorescence specific to each of the nc-1 gene and the internal standard gene is detected. The amount of nec-1 gene is quantified from the amount of fluorescence obtained by detecting a complementary amplification product using a quenching probe and the amount of added internal standard gene. Specifically, soil of a field where planting of root vegetables is planned is collected, bacteria that live in the soil are cultured, and the amount of nec-1 gene is quantified by the above method. The above-described specific gene quantification method that can be used in the present invention is described in detail in JP-A-2002-275 and the like.

  In the process of studying the present inventors, although the effect of applying the above-mentioned microbial material to the field is always recognized when compared with a field not applied, when the microbial material is applied to the field in the same way Even so, it was confirmed that the degree of the effect may be different. And as a result of earnestly examining the cause, it is effective to obtain a stable effect by appropriately controlling the amount of microbial material to be applied according to the amount of common scab inhabiting the soil of the planting field. It came to the conclusion that it is.

  The present inventors first quantified the amount of common scab in the soil to be planted by measuring the amount of the nec-1 gene. And as a result of examining this value and the disease incidence of the crops made in the field, as shown in FIG. 2, the amount of nec-1 gene of scab in the soil to be planted, and the disease incidence of the crops It was confirmed that there is a correlation. Furthermore, depending on the amount of nec-1 gene of the scab of the scab, the amount of the microbial material, which is effective as described above for the root scab, when applied, is changed as described above. Then, we examined whether stable results could be obtained. As a result, the amount of the microbial material may be about 20 kg or more and less than 70 kg when the bacterium is less than 10000 copies due to the amount of nec-1 gene of scab in the soil. It was concluded that it was effective to apply a large amount of microbial material of about 70 kg or more and less than 400 kg at the time of mixing application or sowing at a time of 10,000 copies or more. As described above, if the amount of nec-1 gene in the soil of the field to be planted is first analyzed, and the amount of microbial material applied is controlled according to the analysis value, the root disease common scab pathogenesis The rate can be reduced.

  According to the study by the present inventors, as a result of the above, the microbial material can always function effectively against common scabs inhabiting the soil, and stable scabs in any field. The disease rate can be reduced, good crops can be obtained with a good harvest rate, and continuous cropping obstacles in the field can be overcome. Furthermore, the field after harvest is in a state in which the occurrence of continuous cropping failures is suppressed as compared with the case where the microbial material used in the present invention is not used. As a method of applying the microbial material to the field, after cultivating the field with a cultivator or the like, it may be applied uniformly to the entire surface of the field, or may be applied uniformly to the planting groove. Moreover, the application time can be one week before planting, or can be applied simultaneously with application of chemical fertilizer or the like.

  Furthermore, the present inventors have found that the amount of nec-1 gene of scab in the field soil tends to differ depending on the pH value during the examination process. That is, as shown in FIG. 4, when the pH is 5.5 or more, the amount of nec-1 gene of scab in the soil increases. For this, it is effective to measure the pH value of the soil in the field before application, and if the pH is 5.5 or higher, lower the pH and then apply the microbial material. confirmed. Furthermore, it has been found that it is particularly preferable to use ammonium sulfate for pH adjustment. On the other hand, when the pH value of the soil is lower than 4.0, by adding 40 to 80 kg of lime or mashed lime (powder) per piece, an environment in which antagonistic bacteria in the microbial material can function more effectively is obtained. It is preferable to do.

  Further, according to the study by the present inventors, controlling the phosphoric acid concentration in the soil to an optimum range can be an environmental element that allows the antagonistic bacteria in the microbial material to function more effectively. That is, according to the study by the present inventors, the microbial material used in the present invention is affected by the phosphorus concentration in the soil, and if the phosphorus concentration in the fertilizer is too high, the action of antagonistic bacteria in the microbial material is reduced. However, the effect may not be fully exhibited. Therefore, as a preferred form of the present invention, the phosphoric acid concentration in the soil is measured at the time of the soil analysis, the state of the field is determined, and the fertilizer is fertilized according to the determination, and then the microbial material is applied to the field. Apply. By doing in this way, the phosphorus density | concentration in soil can be made into an optimal state with respect to the microbial material to be used. In addition, what is necessary is just to perform the determination of the phosphoric acid concentration in soil by methods, such as measuring the phosphoric acid concentration or water-soluble phosphoric acid concentration in soil by a well-known method.

  Specifically, for example, the following is performed. The phosphate concentration in the soil is generally required to be about 20 to 40 mg in 1 g of soil. Therefore, the phosphate concentration in the soil is measured, and if the phosphate concentration is surely higher than this, When judged, it is preferable not to fertilize the phosphorus fertilizer and to apply the microbial material in that state. Of course, when it is determined that the phosphoric acid concentration in the soil is lower than the above range, it is necessary to fertilize the phosphorus fertilizer as in the conventional case. When it is delicate whether the phosphoric acid concentration in the soil has reached the above range, the fertilizer is applied in half the normal amount.

  Further, according to the study by the present inventors, when the root vegetable to be planted is a seed pod, the presence or absence of the scab on the seed pod is analyzed in advance, and the scab on the seed pod is present. It is also preferable to disinfect the seed pods and plant the seed pods. For example, in a field where the nec-1 gene of common scab is not present in the soil, the microbial material used in the present invention is a seed pod having 5000 copies of the nec-1 gene and a seed potato having no ec-1 gene. As a result of the examination of the effect, the disease incidence was 0 when using the seed pods without the nec-1 gene, whereas the disease incidence was obtained when using the seed pods with 5000 copies of the nec-1 gene. About 30%, and it was confirmed that disinfection of seed potatoes was effective. For the determination of the presence or absence of the nec-1 gene in the seed pod, the quantitative analysis method for the nec-1 gene described above can of course be used. However, in this case, it is sufficient to confirm the presence of the nec-1 gene. Any analysis method that can qualify the nec-1 gene can be used, not limited to the above method.

  Disinfection of seed husks may be carried out by immersion for 10 to 10 times with agrimycin or the like, but the present invention is not particularly limited thereto.

  In order to further increase the harvest rate, it is preferable to fertilize the field in the same manner as in a normal case. For example, chicken manure compost and a chemical fertilizer with a normal composition suitable for the target root vegetable can be used, although the fertilizer type and component ratio differ depending on the target crop. However, as described above, among the chemical fertilizer components, it is important to control the phosphorus component to an optimum state in order to obtain a high effect by the microbial material used in the present invention. Is preferably determined in relation to the phosphate concentration in the soil, as described above. Furthermore, in the method of the present invention, use of ammonium sulfate as an element for obtaining a high effect by the microbial material used in the present invention can be mentioned. For this reason, as explained above, in order to adjust the pH of the soil in the field, it is preferable to use ammonium sulfate. However, when ammonium sulfate is used, the nitrogen component in the fertilizer is 15 to as the total nitrogen amount. It is preferable to determine the formulation so as to be 26 kg / reverse.

  FIG. 1 shows a system flow of an example of a method for controlling a continuous failure according to the present invention in which the configuration described above is a series. By setting it as such a structure, compared with the case where the microorganism material used by this invention is not used, the onset rate of common scab can be reduced markedly. The system flow of FIG. 1 will be described. First, soil in each field is collected, and then soil diagnosis is performed. In this case, the method according to the present invention is based on measuring at least the density of scab in the soil and determining the amount of the microbial material to be used in the present invention according to the value. Further, as a more preferable form, further soil diagnosis or seed potato diagnosis is performed as necessary, and necessary fertilization, seed sterilization, etc. are determined. After treatment, the microbial material used in the present invention is applied.

Hereinafter, the present invention will be described in more detail with reference to preferred examples and comparative examples.
<Seeds>
Potatoes (variety: Nishitaka) were used as seed seeds for sowing. And the presence or absence of the scab of the seed potato was measured by the nec-1 analysis using the internal standard gene demonstrated previously, and the cultivation test was done using the seed potato in which the scab was not detected.

<Soil in the field>
(1) Based on the above-mentioned knowledge, several fields with a pH of 5.0 were selected as fields for conducting the cultivation test using the potato.
(2) Furthermore, the soil analysis of the above-mentioned field was performed, respectively, and the density of scab in the field was measured by the nec-1 gene amount. A field A having a nec-1 gene amount of about 8,000 copies, a field B having more fungi than a field A having a nec-1 gene amount of about 50,000 copies, and a nec-1 gene amount of 5, Three fields, field C, which has about 000 copies and few bacteria were selected, and each cultivation test was conducted.

<Microbial materials>
Trichoderma asperellum F288 strain was inoculated with 10 kg of wheat bran as a solid medium to 10 ³ cells / g and cultured for 2 weeks. Next, the obtained culture, the wheat bran similar to that used in the above culture, and vermiculite (manufactured by Hiruishi Chemical) are mixed at a mass ratio of about 1: 5: 1. Then, tap water was added and mixed, and the granulated product was used as a microbial material. Microbial materials were applied at the time of sowing seeds.

(Test A)
<Test method>
First, when the phosphoric acid concentration of the soil of the above-mentioned field A and B was determined based on the above-mentioned knowledge, it was confirmed that it was less than usual. These fields were divided, and the amounts of the above-mentioned microbial materials shown in Table 1 were applied to each of the 1a test areas, and then the above-mentioned potato seeds were planted. At that time, 1,000 kg of chicken manure fertilizer / anti and fertilizer [N: P: K = 16: 15: 15 kg (input amount per anti-active ingredient)] were used. Then, seed pods were sown in late August and harvested in late December. Then, the number of harvested potatoes and the number of potatoes that developed common scab were counted, and the disease incidence was determined. The results are shown in Table 1. The onset rate was calculated by assuming that those that were not commercially available due to common scabs.

<Test results>
As shown in Table 1, there was a clear difference in the incidence rate between the cases where the microbial material was applied and the case where the microbial material was not applied, and the effectiveness of the microbial material was confirmed. Moreover, when the amount of nec-1 gene was large, it has confirmed that it was effective to increase the quantity of microbial materials.

(Test B)
<Test method>
When the phosphoric acid concentration of the soil was determined for the field C in which the ance-1 gene amount was 5000 copies, the phosphoric acid concentration was confirmed to be higher than that of the A and B fields. This field C was divided into test sections D and E of 1a. Then, 50 kg of the above-described microbial material was applied to each of the test sections D and E, and further, the fertilizer to be applied to each test section was changed as follows, and a cultivation test was performed. The test area D was fertilized with 1,000 kg of chicken manure fertilizer and chemical fertilizer [N: P: K = 16: 15: 15 kg (input amount of the active ingredient in the opposite direction)]. The fertilizer was applied with 1,000 kg of chicken manure fertilizer and chemical fertilizer [N: P: K = 16: 7: 15 kg (input amount of the active ingredient per anti)]. Then, seed pods were sown in late August for the test sections D and E as described above, and harvested in late December. Then, the number of harvested potatoes and the number of potatoes that developed common scab were counted, and the disease incidence was determined. The results are shown in Table 2. For comparison, the results of the test A performed in the field A where the nce-1 gene amount was the same were also shown. The onset rate was calculated by assuming that those that were not commercially available due to common scabs.

<Test results>
As shown in Table 2, there was a clear difference in the incidence rate between when the microbial material was applied and when it was not applied, confirming the effectiveness of the microbial material. It was confirmed that when the phosphoric acid concentration in the soil increases, the disease incidence increases and the effect of the microbial material tends to decrease.

  The application example of the present invention is applicable to root vegetables such as potato, and when applied to a field with continuous cropping failure, a healthy crop can be stably obtained at a high yield, and the use of agricultural chemicals and chemical fertilizers Since the amount is reduced, the occurrence of continuous cropping failures is reduced, and there is a control method for continuous cropping failures that can realize a shift to environmentally friendly agriculture. The present invention may be applicable to the development of other plants as a technique for utilizing microbial materials.

The system flow of the method concerning this invention. The graph which shows the relationship between the ratio of unhealthy potato and the number of common scabs (nec-1 gene number). The graph which shows the relationship between the ratio of unhealthy potato and phosphoric acid concentration. The graph which shows the relationship between the number of nec-1 genes and pH.

Claims (6)

Trichoderma asperellum F288 strain (deposit number: NITE P-53) showing antagonistic action against Streptomyces s cabies, a pathogen of potato scab.   Microbial material to be applied to the field according to the amount of nec-1 gene obtained by conducting soil analysis of the field before planting root vegetables, measuring the density of scab in the field with the amount of nec-1 gene A control method for continuous cropping disorders, wherein the microbial material comprises at least Trichoderma asperellum F288 strain (deposit number: NITE P-53). Method. Microbial material to be applied to the field according to the amount of nec-1 gene obtained by conducting soil analysis of the field before planting root vegetables, measuring the density of scab in the field with the amount of nec-1 gene A method for controlling continuous cropping disorders, wherein the microbial material comprises at least a solid nutrient medium and a Trichoderma asperellum F288 strain (deposit number: NITE P) in the range of 10 ³ to 10 ⁶ cells per gram of microbial material. -53). A method for controlling a continuous cropping disorder, comprising:   Furthermore, the control method of the continuous cropping disorder of Claim 2 or 3 which fertilizes ammonium sulfate as a fertilizer.   When the root vegetable to be planted is a seed pod, the presence or absence of the scab on the seed pod is analyzed in advance, and if the scab is present in the seed pod, the pod is sterilized and then planted. The control method of the continuous cropping disorder of any one of Claims 2-4.   Furthermore, in the case of the said soil analysis, when the pH of soil is measured, when the pH value of soil is higher than 5.5, ammonium sulfate is applied to a field and the said microbial material is applied in that state. The control method of the continuous cropping failure of any one item | term.

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