JPH04346903A - Composition for preventing agricultural crop disease caused by actinomyces - Google Patents

Abstract

PURPOSE:To provide the subject composition comprising a combination of an N-containing water-soluble polymer obtained by reacting a compound such as an epihalohydrin with an amine, with an alkylenimine polymer and their copolymer, not giving damages to crops, safe for environments and capable of being simply used. CONSTITUTION:The objective composition one kind or more of (A) a nitrogen- containing water-soluble polymer (e.g. a polymer obtained from epichlohydrin and dimethylamine) produced by the reaction of a compound selected from an epoxyhalohydrin of formula I (X is halogen), an alkylene dihalide, a diepoxide of formula II (D does not exist or is alkylene, etc.,) and a dihalogenoalkyl ether with an amine of formula III (R1 to R4 are H, alkyl; A is alkyl; n is 0-5), (B) an alkylenimine polymer (e.g. ethylenimine polymer) and (C) their copolymer as an active ingredients, and is effective for plant diseases caused by the Actinomyces fungi represented by a scab. The composition is effective for crops such as potatoes, sweet potatoes, beets, radishes and carrots.

Description

[Detailed description of the invention]

0001

[Industrial Application Field] The present invention is an agricultural composition for controlling diseases caused by actinomycetes, typified by scab, which occur and cause great damage to potatoes, sweet potatoes, sugar beets, radish, carrots, etc. Regarding.

0002

BACKGROUND OF THE INVENTION Scab disease, which is caused by actinomycetes, occurs on potatoes, sweet potatoes, sugar beets, radish, carrots, etc., and causes great damage. For example, in the case of potatoes, when scab occurs, raised spots appear on the surface of the tuber, which gradually enlarges and becomes rough and crusty, taking on the appearance of a scab. As a result, not only does the appearance become unsightly and the value of the product decreases, but also the starch content of potatoes affected by scab decreases, which is a major blow to producers when producing potatoes as a raw material for starch. .

[0003] Current countermeasures against scab in potatoes, etc. include soaking seeds of potatoes, etc. in a mixture of antibiotics such as streptomycin and copper hydrating agents, and using PCN.
It is sterilized by soaking it in agent B. In addition, soil where scab has occurred is generally fumigated with chloropicrin.

[0004] However, the above-mentioned disinfection of seeds such as potato is not sufficient as a countermeasure against scab, and the disinfection treatment has almost no effect, but since there are no other effective methods, it is only carried out on a casual basis. That is the reality. On the other hand, soil fumigation with chloropicrin has many problems because chloropicrin is highly toxic and volatile. First, when applying the soil, the soil is covered with polyethylene film and fumigated, but since it is not possible to sufficiently isolate it from the outside,
Chloropicrin evaporates to the outside during fumigation, irritates the eyes and bronchial tubes, and is harmful to the human body. As a result, during and several days after the fumigation, people living in the surrounding area had to stay in hotels and other facilities and live far away from the fumigation site, which was inconvenient, and animals that were near the fumigation site developed symptoms of poisoning. There are also reported cases. In addition, it destroys the ecological balance by annihilating microorganisms in the soil, making potatoes more susceptible to diseases after application, and if contaminated potatoes are planted after being fumigated with chloropicrin, it may even lead to more scab disease. There is a report on this (``This Month's Agriculture'', p. 79, April issue [1989]). Furthermore, there is a major problem in that once scab occurs, the same method cannot prevent scab the following year.

[0005] As mentioned above, there are major problems with the conventional medicines used to treat scabies, but since there is no suitable alternative, the above medicines have no choice but to be used. After that, the pH of the soil should be lower than 4.5, which is outside the pH range of 5.5 to 6.5, which is the suitable cultivation condition for potatoes, but outside of the pH range suitable for the growth of scab pathogens. It is cultivated in However, with this method, the yield is significantly lower, with the yield being about half or less. In addition, potatoes are grown once every three years without continuous cropping, but this method cannot be applied in single-crop areas.

[0006]

[Problems to be Solved by the Invention] The present invention has been made in response to the above situation, and an object of the present invention is to provide a drug effective against plant diseases caused by actinomycetes, typified by scab. Yes, and more specifically, there is no phytotoxicity to crops,
Another object of the present invention is to provide the above-mentioned drug, which is safe for the surrounding environment and can be easily applied.

[0007]

[Means and effects for solving the problems] That is, the present invention provides (A) at least one compound selected from the group of epihalohydrin, alkylene dihalide, diepoxide, dihalogenoalkyl ether, and the following general formula:

[
0008

[Case 2]

(R1, R2, R3 and R4 are hydrogen or a straight chain or branched alkyl group having 1 to 3 carbon atoms, A is a straight chain or branched alkyl group having 1 to 6 carbon atoms, and n is a straight chain or branched alkyl group having 1 to 6 carbon atoms. The active ingredient is one or more selected from a nitrogen-containing water-soluble polymer obtained by reaction with an amine represented by (integer), (B) an alkylene imine polymer, and (C) a copolymer thereof. The present invention relates to an agricultural composition for controlling diseases caused by actinomycetes, the composition comprising:

[0010] General formula that is the raw material for the polymer or copolymer that is the active ingredient of the present invention

[0011]

[Chemical formula 3]

(R1, R2, R3 and R4 are hydrogen or a straight chain or branched alkyl group having 1 to 3 carbon atoms, A is a straight chain or branched alkyl group having 1 to 6 carbon atoms, and n is a straight chain or branched alkyl group having 1 to 6 carbon atoms. Examples of amines with monoalkylamines (n=0) include ammonia, methylamine, dimethylamine, diethylamine, dipropylamine, methylethylamine, methylpropylamine, ethylpropylamine, and alkylene polyamines (integer). n=1), ethylenediamine, N,N-
Dimethylethylenediamine, N,N'-dimethylethylenediamine, N,N-diethylethylenediamine, N,
N'-diethylethylenediamine, propylene diamine, N,N-dimethylpropylene diamine, N,N,N'
, N'-tetramethylethylenediamine, etc.
In polyalkylene polyamine (n=2-5),
Examples include diethylenetriamine, triethylenetriamine, and tetraethylenepentamine. These amines can be used in combination within and between the above groups.

[0013] The epihalohydrin that reacts with the above amine has the general formula:

[0014]

[C4]

(X is halogen), and the halogen may be fluorine, chlorine, bromine or iodine, but epichlorohydrin is preferred for economic reasons.

As the alkylene dihalide, the general formula:
Those represented by X-B-X (B is a linear or branched alkylene group of 1 to 20, X is a halogen) are used, and specifically, dichloroethane, dichloropropane, dichlorobutane, dichlorohexane, Examples include dibromoethane, bromochloropropane, dibromopropane, dibromohexane, dichlorononane, and the like. In particular, 1,3-dichloropropane is preferred from the viewpoint of reactivity with amines and economical reasons.

[0017] Furthermore, the diepoxide has the general formula,

[0018]

[C5]

(D is absent or has a carbon number of 1 to
4 straight or branched alkylene group, or -
A group represented by (CH2-O-CH2)m-, where m is 1 to 4) is used, specifically, 1,3-butadiene diepoxide, 1,4-pentadiene diepoxide , 1,5-hexadiene diepoxide, 1,6-peptadiene diepoxide, 1,7
- Octadiene diepoxide, ethylene glycol glycidyl ether, triethylene glycidyl ether, etc.

[0020] The dihalogenoalkyl ether has the general formula: , l is an integer from 1 to 12)
Specifically, when l=1, 2,2'-dichloroethyl ether, 2,2' dibromoethyl ether, 3,3'-dichloropropyl ether, 2,3- Examples include dichloropropyl ether, 4,4'-dichlorobutyl ether, 4,4'-dibromobutyl butyl ether, and when l≧2, ethylene oxide polymer with a degree of polymerization of up to 10 and halogenated alkyl alcohol can be used. Examples include dihalogenoethyl ether, which can be obtained by dehydration reaction and the like. In particular, 2,2'-dichloroethyl ether (hereinafter referred to as dichloroethyl ether) is preferred at least for economic reasons.

The above-mentioned epihalohydrin, alkylene dihalide, diepoxide and dihalogenoalkyl ether may be used individually or in combination of two or more of them. Moreover, when two or more types are mixed, there is no particular limitation as long as the sum of the moles of the mixture is approximately equal to the mole number of the amine.

In order to react the amine with at least one compound selected from the group of epihalohydrin, alkylene dihalide, diepoxide and dihalogenoalkyl ether, a compound selected from the group of epihalohydrin, alkylene dihalide, diepoxide and dihalogenoalkyl ether is used. The sum of the number of moles of one kind of compound selected from the following alone or a mixture and the number of moles of the amine are approximately equal moles,
It is preferable to carry out the reaction using a closed type reaction vessel equipped with a stirrer in an inert gas atmosphere with the internal temperature of the reaction vessel in the range of 30 to 100°C.

More specifically, 20 to 70 of the above-mentioned amine
% aqueous solution was placed in a closed reaction container equipped with a stirrer, reflux condenser, thermometer, etc., the atmosphere inside the container was replaced with nitrogen gas, and the temperature inside the reaction container was adjusted to a predetermined temperature while stirring. , epihalohydrin, alkylene dihalide, diepoxide, and dihalogenoalkyl ether, singly or as a mixture, may be added continuously or batchwise. However, 100% amine can also be used. Water is mainly used as the reaction solvent, but methyl alcohol, ethyl alcohol, dimethylformamide, etc. may also be used in consideration of the solubility of the raw materials and reaction products.

The water-soluble polymer thus obtained is further treated to replace the halogen as counterion (from epihalohydrin, alkylene dihalide and dihalogenoalkyl ether) with another anion, for example SO
4 -- may be used instead, or the counter ion may be removed to convert this polymer into a hydroxide. These water-soluble polymers can also be made into solids by removing the solvent (usually water), and those produced as aqueous solutions can be used as they are, or diluted or concentrated as necessary. You can also do it.

As the alkylene imine polymer, the general formula:

[0026]

[C6]

(R7 is an alkylene group, preferably a linear or branched alkylene group having 1 to 8 carbon atoms. Plural R7's may be the same or different. k is the degree of polymerization of this alkylene imine) ) is used, but these alkyleneimine polymers do not necessarily have a completely linear structure as shown in the general formula above, and may have branches. do not have. Furthermore, a quaternary salt of an alkylene imine polymer obtained by adding hydrochloric acid or the like to an alkylene imine polymer may also be used.

Examples of such alkyleneimine polymers include ethyleneimine polymers, propyleneimine polymers,
Examples include trimethyleneimine polymers, tetramethyleneimine polymers, hexamethyleneimine polymers, copolymers thereof, and mixtures of at least two thereof.

Among these, ethyleneimine polymers are particularly preferred for economical reasons. This product is generally obtained by ring-opening polymerization of ethyleneimine and a cationic catalyst, but it can also be obtained by reaction of ethyleneimine with ethylene dihalide or ammonia, condensation reaction of ethanolamine, etc.

The above amine reactant and alkylene imine polymer are dissolved in a 2 mol/l KBr aqueous solution and measured, and the molecular weight can be judged from the calculated intrinsic viscosity [η] at 25°C. . Since the effect on actinomycetes is stronger in a relatively low molecular weight range, the intrinsic viscosity [η] is 1 dl/g or less, preferably 0.6 dl/g.
Below, it is more preferably 0.4 dl/g or less, but it is not particularly limited to this range.

The active ingredient of the present invention can be used not only in the above-mentioned polymers but also in copolymers. That is, a copolymer obtained by reacting at least one compound selected from the group of epihalohydrin, alkylene dihalide, diepoxide, and dihalogenoalkyl ether with an amine represented by the above general formula, an alkylene imine copolymer, and the above-mentioned It may be a polymer or a copolymer of the above-mentioned copolymer and the above-mentioned alkylene imine polymer.

Next, the method of applying the composition of the present invention will be explained. When cultivating crops that are susceptible to actinomycetes, such as scab, such as potatoes, the first step in planting crops is
The active ingredients of the present invention, alone or in a mixture, are diluted 1/10 to 1/10,000 with water and other solvents into the soil at an appropriate time from ~2 months in advance to just before planting.
Alternatively, it can be mixed with other agricultural chemicals, fertilizers, etc., and mixed into the soil by spraying or dripping. The application amount of the present composition is economically appropriate to be 0.2 to 20 kg per 10 ares, but it is not limited to this range.

[0033] When spraying on soil, the aqueous solution of the present composition may be mixed relatively thickly into the soil itself, or absorbed into magnesia lime, calcium carbonate, chemical fertilizers, and other inorganic substances such as diatomaceous earth or zeolite. Another method is to mix it into the cultivation soil afterwards.

As an application method during cultivation and growth, there is also a method of spraying the above aqueous solution, but since it is easily absorbed only on the soil surface, it is necessary to make a hole during spraying or use a pipe called "tube injection" to spray it into the soil. It is better to include it in The application amount is economically appropriate within the above range. Although it may be used in the same manner as above, it may be mixed with magnesia lime or the like, but it is more effective to incorporate it into the soil.

Furthermore, the active ingredient of the present invention is diluted to 1/10 to 1/10,000 with a solvent such as water, or mixed with other agricultural chemicals, to crop seeds or seed potatoes.
Bringing seeds, etc. into sufficient contact with chemicals by soaking or applying them is also effective in preventing diseases.

[0036] The usage rate of the present composition in soil mixing and seed disinfection is not limited to a limited range, but
A small amount is enough to have an effect, so 1/50 to 1/1000
It is appropriate to use a certain amount.

When the composition of the present invention is applied in this manner, scab and other plant diseases caused by actinomycetes can be easily suppressed, and crop yields are increased. In addition, in order to avoid diseases, conditions that are not suitable for cultivation (
Since there is no need to cultivate at different pH levels, etc., the yield can be increased in this respect as well, and continuous cultivation is also possible. Such effects have never been achieved with conventional drugs, and the composition of the present invention can be said to be completely innovative against diseases caused by actinomycetes.

Furthermore, the present invention also has the following effects and has extremely high practical value. (1) Since the composition of the present invention is substantially non-volatile, there is no need to pay attention to the safety of the surroundings during use. (2) The composition of the present invention has no effect on the germination rate, leaf bud rate, etc. of crops, and has no adverse effects on cultivation. (3) The composition of the present invention is extremely safe, non-irritating to warm-blooded animals, and extremely easy to use. (4) The composition of the present invention is substantially harmless to useful soil microorganisms and does not disrupt the balance of soil microorganisms, so that crops can be grown using normal cultivation methods. Although the active ingredients of the composition of the present invention are known as sedimentation accelerators and flocculants, such effects on agricultural crops were completely unexpected.

The effects of the composition of the present invention have been described above on scab diseases that occur in crops such as potatoes, sugar beets, radish, carrots, etc. Diseases can be controlled.

[0040] Recently, there are various methods for cultivating crops, so not only in soil cultivation but also in hydroponic cultivation and gravel cultivation, for example, seed sterilization and small additions to supplementary water, circulating water, etc. By doing so, it is possible to prevent the outbreak of diseases caused by actinomycetes.

[0041]

[Examples] The present invention will be specifically explained below with reference to Examples. Example 1. The effect on Streptomyces and other microorganisms was shown in Example 2. Regarding the results of the pot test in Example 3. This section explains the evaluation results at the experimental farm.

First, the method for preparing the water-soluble polymer used in each example and the method for preparing the mixture containing the water-soluble polymer will be explained. (1) Sample A The molar ratio of dimethylamine and epichlorohydrin is 1:1
Add epichlorohydrin to a 50% dimethylamine aqueous solution while maintaining the reaction temperature at 85°C, and stir and mix until the ratio becomes 2 mol/l.
The intrinsic viscosity [η] measured with a KBr aqueous solution is 0.11 d.
1/g composition) was diluted with water to obtain a purity of 50% by weight, which was designated as Sample A.

(2) Sample B A 40% aqueous solution of a mixture of dimethylamine and ammonia prepared in advance at a molar ratio of 1:0.1, and epichlorohydrin prepared in advance at a molar ratio of 1:0.5. The molar ratio of the mixture of propylene dichloride is approximately 1:
1, a mixture of epichlorohydrin and propylene dichloride was added to a 40% aqueous solution of the amine compound while maintaining the reaction temperature at 90°C, and mixed with stirring until the resulting mixed reaction composition (2 mol/l Sample B was prepared by diluting a composition with an intrinsic viscosity [η] of 0.16 dl/g as measured with a KBr aqueous solution with water to obtain a purity of 50% by weight.

(3) Sample C A 50% aqueous solution of a mixture of dimethylamine and N,N,N',N',-tetramethylethylenediamine prepared in advance at a molar ratio of 1:0.3 and 0.5
A mixture of ethylene glycol glycidyl ether and dichloroethyl ether was added to the mixed amine aqueous solution at a reaction temperature until the molar ratio of the mixture of ethylene glycol glycidyl ether and dichloroethyl ether prepared at a ratio of 1:1 was approximately 1:1. The mixed reaction composition (2 mol/l of KB
Intrinsic viscosity [η] measured in r aqueous solution is 0.33 dl/g
A sample C was prepared by diluting the composition (composition) with water to obtain a purity of 50% by weight.

(4) Sample D A 50% aqueous solution of a mixture of dimethylamine and N,N,N',N',-tetramethylethylenediamine prepared in advance at a molar ratio of 1:0.3 and 1;1
A mixture of epichlorohydrin and propylene dichloride was added to the mixed amine aqueous solution while maintaining the reaction temperature at 80°C, and the mixture was stirred until the molar ratio of the mixture of epichlorohydrin and propylene dichloride was approximately 1:1. , the obtained mixed reaction composition (intrinsic viscosity [η] measured with a 2 mol/l KBr aqueous solution was 0.2
Sample D was obtained by diluting 3 dl/g of the composition) with water to obtain a purity of 50% by weight.

(5) Sample E Commercially available polyethyleneimine having branches containing primary, secondary, and tertiary amino nitrogen (2 mol/
The intrinsic viscosity [η] measured with a KBr aqueous solution of 0.09
dl/g composition) was diluted with water to obtain a purity of 50% by weight, which was designated as Sample E.

[0047]

[C7]

(6) Sample F This sample A was sprinkled and mixed with calcium carbonate for soil mixing, and dried at 60°C to prepare a sample with a ratio of this composition:calcium carbonate of 1:9. It was set as F.

(7) Sample G A dimethylamine-epichlorohydrin copolymer with an intrinsic viscosity [η] of 1.12 dl/g measured with a 2 mol/l KBr aqueous solution was obtained, and this was diluted with water. A sample with a purity of 50% by weight was designated as sample G.

Example 1. Effect on Streptomyces and other microorganisms From the National Institute of Agricultural and Biological Resources, Ministry of Agriculture, Forestry and Fisheries Streptomyce
s MAFF 03-01524 was obtained, and the bacteria were St.
aphylococcus aureus ATCC
6538 and Pseudomonas aerugin
osa P2, and the fungus Aureobasidiu
m H2627 and Candida albicans
MIC (minimum inhibitory concentration) and MCC (minimum bactericidal concentration) in the culture medium were measured using IFO1594.

Preparation of inoculum solution Actinobacteria: yeast extract/starch medium (yeast extract 2g,
10g soluble starch, 1,000ml distilled water, ph
= 7.0) at 115°C for 20 minutes,
The above actinomycetes were cultured at 25°C for 5 days. Bacteria: precultured in 5 ml of SCD broth at 35°C for 24 hours,
A test bacterial solution was prepared with a bacterial count of approximately 10 6 /ml. Yeast: Precultured in 5 ml of GP broth at 35°C for 48 hours, adjusted to a cell count of 1 x 106/ml with physiological saline, and used as a test bacterial solution. Mold: Inoculated on potato dextrose slant medium, 30
Preculture was carried out at ℃ for 10 days. 0.05% Tw on that slope
Add een80 physiological saline, and the number of spores is 1 x 106/m.
This was used as a test bacterial solution.

Antibacterial activity measurement method Actinomycetes, bacteria, and fungi were tested using liquid medium dilution method
C, MCC was measured. A 2-fold dilution series was prepared by adding predetermined amounts of the samples A to E above, using the medium described above. The volume of the medium was 2 ml. 0.1 ml of the above test bacterial solution was inoculated into each test tube, and actinomycetes were cultured at 25°C for 5 days, bacteria and yeast were cultured at 35°C for 48 hours, and molds were cultured at 30°C for 7 days. The MIC value was determined as the minimum sample concentration in the test tube at which no turbidity was observed. Furthermore, from each test tube that did not show turbidity, one platinum loopful was inoculated into each medium without sample addition, and the actinomycetes were incubated at 25℃ for 5 days.
After culturing for 48 hours at 35° C. for bacteria and yeast and 7 days at 30° C. for mold, the MCC value was determined based on the minimum concentration of the sample in the test tube at which no turbidity was observed.

[0053]

[Table 1]

Example 2. Pot test After mixing granular chemical fertilizer and calcium carbonate (0.3% by weight) into soil contaminated with scab, 7 kg of each
was placed in a pot with a diameter of 30 cm, and an aqueous solution of each of the above samples with a purity of 1/200, except for sample F, was sprayed onto the soil in the pot and mixed with the soil. The concentration was adjusted to 60 ppm. The pots containing each sample were numbered from pot A to pot G in the same way as the sample name. In addition, 8.4 g of sample F was added to the above 7 kg of soil, mixed well, and this pot was named pot F. In addition, a pot containing no sample was prepared, and this pot was used as a control. In addition, each pot contains 3 of the same items.
(n=3).

The pots prepared as described above were allowed to stand for two weeks, and then potatoes whose seeds had been sterilized with streptomycin and a copper hydrating agent were planted as seed potatoes (Yutaka species 2). Pots were placed outdoors to grow, and 70 days after planting, the tubers were dug up and tubers weighing approximately 15 g or more were examined. (the value obtained by dividing the harvested potatoes by the amount of potatoes harvested), and the lesion area ratio (the value obtained by dividing the lesion area by the amount of potatoes harvested).

[0056]

[Table 2]

Example 3. Evaluation at an experimental farm A granular chemical fertilizer and calcium carbonate (0.3% by weight) were mixed in an area where scabies were most prevalent, and each area was divided into 6 m2 areas to serve as experimental plots. Experimental plots 1 to 21 were taken, and 3 plots were randomly selected, with the same conditions as test plots 1 to 7.

[0058] Test plot 1 was used as a control, and the furrows were raised in the same manner as in the general cultivation method, and potatoes whose seeds had been sterilized with streptomycin and a copper hydrating agent were planted as seed potatoes (2 types of Yutaka) in the furrows.

Test area 2 was prepared by diluting sample A with water to obtain a purity of 1.
/200 aqueous solution per experimental area 7
Plow while spraying 20g to mix sample A into the soil.
After 3 weeks, the ridges were raised and planted in the same manner as in test plot 1.

Test group 3 was prepared by diluting sample A with water to obtain a purity of 1.
/200 aqueous solution, 1 per experimental area.
Sample A was mixed into the soil by plowing while dispersing 440 g of the soil in the same manner as in test plot 2.

Test plot 4 was the same as test plot 1. However, two weeks after planting, the aqueous solution used in Test Area 2 was injected into the soil (inserting a tube into the soil and pouring the aqueous solution near where the potatoes were planted). Usage amount is 14
40g.

Test area 5 was prepared by diluting sample D with water to obtain a purity of 1.
/200 aqueous solution per experimental area 7
Plow while spraying 20g to mix sample D into the soil,
After 3 weeks, the ridges were raised and planted in the same manner as in test plot 1.

For test plot 6, 900 g of sample F was added to the soil per test plot, mixed well, and after 3 weeks, the ridges were raised and planted in the same manner as test plot 1.

In test area 7, sample G was diluted with water to obtain a purity of 1.
/200 aqueous solution per experimental area 7
Plow while spraying 20g to mix sample G into the soil,
The same procedure as in test group 5 was conducted.

In each test plot, two weeks after planting, the ridges were covered with polyethylene sheets and mulched. Then, three months after planting, the tubers were dug up and the tubers weighing more than 20 g were examined to determine the total number of tubers, the diseased tuber rate (even if there is a lesion about 2 mm in size, it is considered a diseased potato), and the lesion area rate ( The value obtained by dividing the lesion area by the amount of harvested potatoes was calculated.

[0066]

[Table 3]

[0067]

Effects of the Invention As explained above, the composition of the present invention has an outstanding control effect on plant diseases caused by actinomycetes, typified by scab, and is not as toxic as conventional scab drugs. Since it is neither strong nor volatile, it is highly safe to the surrounding environment and easy to apply. Furthermore, it does not cause phytotoxicity to cultivated crops.

Claims (1)

[Claims] Claim 1: (A) at least one compound selected from the group of epihalohydrin, alkylene dihalide, diepoxide, and dihalogenoalkyl ether; A straight chain or branched alkyl group having 1 to 3 carbon atoms, A is a straight chain or branched alkyl group having 1 to 6 carbon atoms, n is 0 to
(B) an alkylene imine polymer, and (C) one or more of these copolymers. An agricultural composition for controlling diseases caused by actinomycetes, the composition containing as an active ingredient.